Cell Analysis

ZE5 Cell Analyzer

Superior performance and flexibility to
align with your flow cytometry needs.

INTRODUCING THE

ZE5 CELL ANALYZER

The first of its kind, the ZE5 Cell Analyzer is built around an integrated plate loader. It was
designed with researchers in mind and provides superior performance and flexibility to
help streamline your workflow.

BIO-RAD LABORATORIES, INC.

LOADER

High Throughput

 ully automated acquisition enables

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sampling from 5 ml tube racks and any
type of microtiter plate up to 384 wells
including deep well plates and custom
configurations, giving you the flexibility
you need
 atent pending automatic probe calibration
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and built-in crash detection increase up
time and reduce service needs
Integrated, programmable wash station
reduces carryover and time between
samples, improving the quality of your
data and increasing the capacity of
your lab

 ime-saving high-throughput mode

T
can analyze a 96-well plate with <0.5%
carryover in less than 15 minutes
 ctive temperature control maintains
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the integrity of your samples from start
to finish
 rue plate shaker agitation resuspends
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your samples, reducing clogs
 olumetric sample uptake allows
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automatic concentration calculation and
absolute counting without beads
 o dead volume requirement, no wasted
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aspirated sample, and the ability to
automatically return unused sample to the
tube or well eliminates wasted sample

 ingle tube load position provides the

S
flexibility to interrupt a plate, run one
sample, and resume the microtiter plate to
streamline workflow
 dd reagents to your samples in real-time
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for kinetic experiments
 n-board calibration beads provide
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automated quality control (QC) without
user intervention to reduce setup time
 olume tracking for on-board beads
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provides an alert when replacement is
needed and helps conserve usage to
reduce consumable cost
Innovative continuous sampling allows
unlimited volume acquisition at a stable
flow rate

BIO-RAD.COM

OPTICS

Scalable and Sensitive

 E5 offers free-space excitation with

Z
up to five spatially separated lasers
ranging from 355 nm to 640 nm, allowing
optimal wavelength selection for your
multicolor experiments
Integrated beam shaping and focusing
optics for each laser increase excitation
stability
 ith up to 28 fluorescence detectors,
W
ZE5 has out-of-the-box flexibility for your
multicolor applications

 apidly verifying the optical filter

R
configuration and tracking the detection
performance over time, the patent
pending ZE5-EYE profiles your instrument
with 10 distinct wavelengths of LEDs
 E5-EYE technology enables
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system-to-system detector calibration
giving you consistent results between
multiple instruments

355/375 nm

405 nm

488 nm

561 nm

640 nm

BIO-RAD LABORATORIES, INC.

 ual forward scatter (FSC) design

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allows standard FSC detection while
simultaneously collecting small particle or
alternate wavelength scatter data
 ield upgrades are simplified
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with modular excitation and
detection components

FLUIDICS + ELECTRONICS

Innovative and Integrated

Patent pending sample delivery system allows stable, continuous

Uncompromised Accuracy

flow from 0.0025 to 2.5 l/sec and provides a direct sample path
to the flow cell

 igh velocity sheath flow delivers unparalleled analytical

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throughput to collect more data in less time

Hot-swappable bulk fluid bottles allow uninterrupted system use

 n-board cleaner and additive bottles allow dilution for sheath
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fluid with an antimicrobial and surfactant as well as hands-free
automated cleaning and rinsing
 idirectional flow to the cuvette provides built-in high pressure
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unclogging and cleaning capabilities

 easured peak, area, and width parameters available

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simultaneously for every channel to accurately characterize
samples
 roprietary digital processing with 24-bit data maximizes dynamic
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range and reduces channel noise for superior dim population
resolution
 ero dead-time processing with dynamic window extension
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ensures full data collection for every sample
 assively parallel, pipelined architecture eliminates hard aborts to
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provide more data in less time
 nassisted laser delay system configures optimal delays with no
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user interaction
 eal-time hardware system control enables accurate, repeatable
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sampler operation

BIO-RAD.COM

APPLICATION DATA

Consistent and Reliable

An optical filter layout with consistent path lengths yields optimal detection and sensitivity for all wavelengths at each detector.

134

127

0
10 0 101 102 103 104
FITC

CD8 PE

Count

Count

304
152

8-peak bead resolution in key channels. FITC, fluorescein

isothiocyanate; PE, phycoerythrin; APC, allophycocyanin.

BIO-RAD LABORATORIES, INC.

128

10 0 101 102 103 104

CD3 Brilliant Violet 421

105

10 0 101 102 103 104

CD4 APC

105

104

CD8+ lymphocytes

103
102
101
10 0

0
10 0 101 102 103 104
APC

102

105

64

0
10 0 101 102 103 104
Pacific Blue

CD4+ lymphocytes

10 0
10 0 101 102 103 104
CD45 PE-Cy5

192

103

101

456

161

Lymphocytes

64

10 0 101 102 103 104

PE

483
322

128

CD4 APC

254

104

192

CD8 PE

268

488 nm SSC

381
Count

Count

402

10 0 101 102 103 104 105

CD3 Brilliant Violet 421

Four-color panel run on a 5-laser ZE5 with a 355 nm, 405 nm, 488 nm,
561 nm, and 640 nm laser configuration. The panel shown requires no
compensation and defines human T-cell subsets using lysed whole blood from
a normal donor. APC, allophycocyanin; PE, phycoerythrin; SSC, side scatter.

SOFTWARE

Intuitive and Powerful

Scheduled startup and quality control

eliminates waiting for system warm up

 legant and intuitive user interface with

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quick action buttons of suggested next
steps to simplify use
 luorochrome selector assists with
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experiment design and worklist setup
giving users a head start
 djustable region based event monitoring
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ensures the quality of your data from well
to well
 cquire and store files with up to 100
A
million events with all parameters enabled
at high speed, reducing rare event
detection time

 nalyze saved files while acquiring new

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data to improve your workflow and reduce
analysis-only time on your systems
 atent pending Threshold plot shows
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you everything the electronics see to give
you full confidence in setting the proper
threshold
 ultichannel triggering gives the option to
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use a combination of inputs for complex
experiments
 ustom heat map display gives a visual
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presentation of acquired data for plates
and tube racks to quickly and clearly
summarize experimental results

 nalysis features including overlays, colorA

gating, and ratio provide the capability to
display data in the way you choose
 atch analysis allows quick summaries
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and reports for multiple samples reducing
the tedious work of manually opening and
analyzing individual files
 ffline mode lets you create runlists and
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experimental design from your desktop
before sitting down at the system
 utomation-ready with application
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programming interfaces (API) for integration
with robotics or custom analytical systems

BIO-RAD.COM

Specifications
System
Fluorescence sensitivity

<100 MESF for FITC, PE, APC

Scatter sensitivity

<0.2 m FSC resolution with small particle detection module

Loader

Integrated sample loader with agitation and temperature control. Up to 384 well plates; 40 tube
rack for 5 ml 12 x 75 mm tube loading. Stat tube position for single tube loading

Optics
Excitation

Up to five spatially separated lasers. Standard options include:

355 nm, 50 mW
405 nm, 100 mW
561 nm, 50 mW
375 nm, 50 mW
488 nm, 60 mW
640 nm, 100 mW

Detection

Up to 30 detectors including FSC and SSC; optional second FSC detector

Cuvette

Fused silica with 145 x 265 m channel

Electronics
Speed

>100,000 events per second with all parameters enabled

Data processing

Simultaneous measured peak, area, and width for every channel. 24-bit data for peak and area.
17-bit data for width with high-resolution linear interpolation at the half height.

Fluidics
Sample flow rates

0.00252.5 l/sec

Bulk fluids

4 x 4 L bulk fluid tanks on board for sheath and waste. On board additive concentrate
and cleaner.

Installation
Power

95240 VAC, 5060 Hz, <500W

Dimensions (W x D x H)

29 x 27 x 26 in.; 74 x 69 x 66 cm

Weight

<200 lbs; <90 kg

Temperature
and humidity
Air and vacuum supply

1825C; 2060% RH

Software
Operating system

Included, on-board
Windows 7 Pro

Flow Cytometry
Standard (FCS) format

FCS 3.1

QC

Automated quality control with on-board calibration beads

Workstation

Intel Core i5-4590 Quad Core; 3.3 GHz; 8 GB RAM DDR 1600 MHz; 1 TB storage

Regulatory

CE, Class I (1) laser product

Research use only

Brilliant Violet 421 is a trademark of Sirigen Group Ltd. Cy is a trademark of GE Healthcare.

Intel Core is a trademark of Intel Corporation. Pacific Blue is a trademark of Life Technologies
Corporation. Windows is a trademark of Microsoft Corporation.

Bio-Rad
Laboratories, Inc.
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Life Science
Group

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