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CONTROL OF MICROBIAL GROWTH

GROUP 4: 2B1

CO, Peter Sam CONCEPCION, Fatima Grace

DEL ROSARIO, Jenna DEMDAM, Michael

PART 1: Physical Control (PCA Plates)


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Results:

Specimen Temp. Set Up Control 10 MINS 20 MINS 30 MINS


Staphylococcus 60 + + + +
100 + - - -
aureus
(group 4 results)
Escherichia coli 60 + + + +
100 + + + +
(group 3 results)

Part 2: Chemical Control (NB-4.5mL)


TTuubbee0123
Results:

Specimen Disinfectant
Tube4
Control UNDILUTE 1:10 (T2) 1:100 (T3) 1:1000
(T0) D (T1) (T4)
Staphylocc 40% +, with +, with +, with +, with +, with
ocus aureus isopropyl sediment sediments sediment sediment sediment
(group 4 alcohol s (similar to s s s
results) T0) (slightly (slightly
less less
turbid turbid
than T1) than T2)
Escherichia 70% +, with +, with +, with +, with +, with
coli (group isopropyl sediment sediments sediment sediment sediment
3 results) alcohol s s s s
Part 3: Use of Antibiotic Discs (MHA Plates)

S3 2 4 h r
wM oH l d
aA b b a c t e r i
ip n l a a t l
Me c su l t u r e
( N B )
Ha n t i
Ab i o t
Pi c l s
a t
e s
Results: Group 4 Staphylococcus aureus
Antibiotics Zone of Inhibition Comments
OX (Oxacillin) 1st 0.8cm Observed penetration in ZOI
2nd 0.9cm -static
3rd 0.10cm
SXT(Trimethoprim 1st 2.9cm No penetration
sulfamethoxatole) 2nd 3.0cm -cidal
3rd3.1cm
Lysol (1:10) A 1st 2.9cm No penetration
2nd 3.8cm -cidal
3rd 2.9cm
Lysol (1:10) B 1st 3.6cm No penetration
2nd 3.0cm -cidal
3rd 3.8cm
Results: Group 3 Escherichia coli

Antibiotics Zone of Inhibition Comments


Tobramycin 1st 1.1 cm -cidal
2nd 1.1cm -cidal
3rd 1.1cm -cidal
Ceffazidime 1st 2.6 cm -static
2nd 2.9 cm -static
3rd 2.7 cm -static
Lemon A 1st 2 cm -cidal
2nd 2 cm -cidal
3rd 1.9cm -cidal
Lemon B 1st 1.7 cm -cidal
2nd 1.7 cm -cidal
3rd 1.6 cm -cidal
Based on group 4s results, it can be observed that Oxacillin was the least effective, evident with the
smallest zones of inhibition. Moreover, growth of colonies were observed inside the zone of inhibition,
indicating that it does not completely kill microorganisms and may just slightly inhibit the growth of the
bacteria within a given time. These results for OX tests on S. aureus can be attributed to the existence of
MRSA and ORSA. Despite having OX as an antibiotic in the penicillin groups known to treat
staphylococcus bacteria caused infections, some strains of S. aureus have become resistant to some of
these antibiotics. These are known as either Methicillin Resistant Staphylococcus aureus or Oxacillin
Resistant Staphylococcus aureus. On the other hand, a large ZOI can be observed under both SXT and
Lysol discs. Trimethoprim-sulfamethoxazole is a known antibiotic used to fight bacteria including the
mentioned MRSA and ORSA. SXT targets the folic acid synthesis process of bacteria, and thus is able to
inhibit the growth of S. aureus around the discs. Lysol can be considered as cidal evident with large
ZOIs, indicating that it was not only able to inhibit growth but more of kill the colonies present. This can
be attributed to the N-Alkyl dimethyl benzyl ammonium chloride present in Lysol, being the active
antimicrobial component which can cause bacterial death by targeting and affecting the cell membrane,
lipid bilayer, as well as other intramolecular processes.

References:

http://www.rbnainfo.com/productpro/ProductSearch.do?
brandId=19&productLineId=344&searchType=PL&template=1

http://hubpages.com/education/The-Science-Behind-Household-Cleaners

http://aac.asm.org/content/54/3/1226.full