Sampel

HIDROLISIS PATI pH 6.0, Termamyl,

95-100 oC, 30 menit

HIDROLISIS PROTEIN pH 7.5, protease,

60 C, 30 menit
o

HIDROLISIS PATI pH 4.0-4.6,

amyloglukosidase,
60 oC, 30 menit

PRESIPITASI DF EtOH 95%,

Suhu kamar, 60 menit

FILTRASI Porositas 2, celite 0.5 g

Residu

PENCUCIAN RESIDU 3 x 20 ml ETOH 78%

2 x 10 ml ETOH 95%
1 x 10 ml Acetone

PENGERINGAN 70 oC vacum, semalam

atau
BERAT KONSTAN 105 oC , 5 jam.

Total DF Koreksi kadar protein & abu

DIAGRAM ALIR PENENTUAN TOTAL DF (AOAC,
1995)
Sampel + guargum

HIDROLISIS PROTEIN pepsin HCl, 37 oC,

30 menit

HIDROLISIS PATI buffer asetat, 37 oC

Invertase, amylase,
Amyloglukosidase

INKUBASI 20 MENIT PEMISAHAN EtOH

66%
GLUKOSA
Centrifugasi

PENENTUAN GLUKOSA
RAG

INKUBASI 120 MENIT PEMISAHAN EtOH

66%
GLUKOSA
Centrifugasi

PENENTUAN GLUKOSA
G120

HIDROLISIS PATI 100 oC , 30 menit

GELATINISASI RS 2M KOH, 0 oC, 30 menit

HIDROLISIS RS as. asetat,

amyloglukosidase
70 oC, 30 menit

INAKTIVASI ENSIM 100 oC , 10 menit

PEMISAHAN GLUKOSA dingin, pengenceran

 sentrifugasi

PENENTUAN GLUKOSA Total Glukosa (TG)

RS = 0.9 (TG-
G120)

DIAGRAM ALIR ANALISIS RS (Englyst et. al.,

1992)
Sampel

EKSTRAKSI GULA EtOH 80%

EKSTRAKSI LEMAK Petroleom ether

HIDROLISIS PATI (I) 95-100 oC , 30 menit

Amylase

HIDROLISIS PATI (II) 37-40 oC , semalam,

amylogluko
-sidase, pullulanase

INAKTIVASI ENSIM mendidih, 15 menit

PRESIPITASI TDF & RS sentrifugasi dingin

Endapan (TDF & RS) Supernatan (glukosa/pati)

HIDROLISIS RS (I) 2 M KOH, 30 menit

Amylase

HIDROLISIS RS (II) 37-40 oC , semalam,

Amylo
glukosidase, pullulanase

INAKTIVASI ENSIM mendidih, 15

menit

PRESIPITASI TDF sentrifugasi dingin

Supernatan (glukosa) Endapan (TDF)

PENENTUAN GLUKOSA Spektrofotometri (Nelson)

Glukosa (RS = 0,9 x glukosa)

DIAGRAM ALIR PENENTUAN RS

(Englyst & Cummings, 1987)

Sampel, basis: 1 g total pati

PENGUNYAHAN 15 kali, 15 detik

INKUBASI I pepsin, pH 1.5 (+HCl)

37 OC, 30 menit

INKUBASI II pankreatin,
amyloglukosidase
pH 5.0; 40 OC , 16 jam

PRESIPITASI EtOH 95%, 4 x volume

60 menit

FILTRASI porositas 2(40-90 um)

0.5 g celite

Filtrat

Analisis GLUKOSA Pati

Endapan Analisis N + abu % Protein & abu

GELATINISASI RS 2M KOH, 30 menit

HIDROLISIS RS Amyloglukosidase

PENENTUAN GLUKOSA RS = 0,90 x

glukosa

DIAGRAM ALIR ANALISIS RS (Akerberg et al.,

1998)

Sampel

HIDROLISIS PATI pH 6.0, Termamyl,

95-100 oC, 15 (30) mnt

HIDROLISIS PROTEIN pH 7.5, protease,

60 C, 30 menit
o

HIDROLISIS PATI pH 4.0-4.6, amylogluko-

sidase, 60 oC, 30 mnt

PENCUCIAN SDF 2 x 10 ml air

2 x 10 ml EtOH 95%
1 x 10 ml Acetone

Residu (IDF)

PENGERINGAN 70 oC vacum, semalam

BERAT KONSTAN atau 105 oC , 5 jam.
 IDF Koreksi kadar protein &
abu

DIAGRAM ALIR PENENTUAN INSOLUBLE

DIETARY FIBRE (IDF) , AOAC, 1995

Sampel

HIDROLISIS PATI MES-TRIS Buffer (pH 8.2,

- amilase, 95-100 oC,
15 (35) menit

HIDROLISIS PROTEIN pH 7.5, protease,

60 C, 30 menit
o

HIDROLISIS PATI pH 4.0-4.7, amylogluko-

sidase, 60 oC, 30 menit

FILTRASI Filtrat SDF

Residu (IDF)
Air cucian

PENCUCIAN I 2 x 10 ml air 70 oC

PENCUCIAN II 2 x 15 ml ETOH 78%

2 x 15 ml ETOH 95%
 2 x 15 ml aseton

PENGERINGAN 70 oC vacum, semalam

(bobot konstan) atau 105 oC , 5 jam.

IDF Koreksi kadar protein &

abu

DIAGRAM ALIR PENENTUAN SDF DAN IDF,

(AOAC, 1995) .