PJ BS Bosxiczs so Pakistan Journal of Biological Sciences ANSIez n Network for Scientific Information 308 Lasani Towns Sargodha Road, Faisalabad - PakistanPakistan Journal of Biological Sciences § (11): 1181-1188, 2002 © Aslan Network for Sclentitie Information 2002 Effect of Application of Biofertilizer on the Yield and NPK Uptake of Some Wheat Genotypes as Affected by the Biological Properties of Soil MLL ELHawary,'l ElHawary. Fatma, “AM, ELGhamry and °E. E-Naggar Department of Seed Technology Research, F.C.R.I., ARC Giza, Eaypt ‘Department of Microbiology, ‘Departmert of Soil Science, Faculty of Agriulture, Mansoura University, El-Mansoura, Egypt “Egyptian Fertilizer Development Center, ELMansoura, Egypt ‘Abwraen The effet of sere whee penchype qi Woes wlth Azowpalam Braafenre and Assrisebim cavinadanee fon west yield ane NPK uptake was evaluated under diferent lve of liquid Nr-feritzation (Zero, 60 snd TOO from the ‘commanded N Rac. Inoculation vith any bistertie eats te considrsle srprovernert in hast gr aed aw yn {= compares with thar eapective conte. Hoviever response aifered scouring (2 the type of batertazera. The suptenly Felted Yo Azamizodium ineclton vias clear en gran wad folowed by nocuam conanng 2 rxture of Azorizebum 2nd [Eieepei,athough toe use af Aoapariam gave the loeetsespant, bat vas vl her than the wonocaled plants (contol resin levis of ritogen caused ncfese nthe nhest pram ad stan yids, but the response was femasly high a 100% N. The aferences i ran veld became mich lower winen Azoeperilm in combination with decreasing eves of Metecizer ware used. The relsbve increase vas 48% Ivan Acorizebium + 100% N ware Ueed. atthe same tine the ‘ppication of 100% N exceeded usng bath 100% N+ Azosperllum (13.5%) and 100% N+ mitre of neuen 12 845%). epleasen of 100% N rose vn Asohzebum * Azosperlum inaculum gave 2 ponve elect snd alzattcted the 1000 fare weigh. Thi eapane vated arearang tothe wheat vatietie especay wth Saha 8 ohich gst the Nighes! response. Bitronces in NPK upto depend en whest genotype end on ferent macobal eatments, The appieaton of Aaa/hizblum TYO0% N enhanced the mineral uate falewed by miture st 180% Ns [Asaspriam © Aethabied acaba Key word: Bloeriser,Azozparilum braianse, Azorizehum couinodenee, noculaton. wheat Introduction heat is the most important cereal ctop in Egypt. Gecaure of Incressing. human demand fa tod, atenpts, ate made utvate mere mea wh igh viekéng varieties, In edation fo using the recommended culutal practices to maximize the wheat Droductsty to mat the natal needs. Alhough mitogen Ie an Important nutient in eterent oi" types, application has ‘Thue wae of toi nittogenfeton by ving Neier wl help ‘sha to improve pont grow to Gereuse the proauction cost ‘ind ensrcomantal ak (Aly oa, 1996; Shatral ane Hes, 7988, ElMiwary, 1200), Factors controling success colonzation By Azeapiilrn aed Aeorisabim apecien are woh yet My ‘ndertoed, bot t has been suggested that polar fagall and ‘Esface palysoceharkes re involved in tis process (Gao an Fendt, 1984), The mechaniam fr aucoesstul norton fe all Cninonn,athaugh some avenues Mave bean suggested in the ‘ipeiments (Oren 1086). Theefere Is procteally posable to Simulate the exact condone and factors afectng. fil ‘ipetiment results Aastonay, toot clenzaton can et yet be ‘rhances by ssteralappeatin of beefaal bate [DeDbeaere ft). 1800), becnuse the sol atts ars biciogical butter aganet ‘ott of the nenresdont bacteria Bashan, 1866). ‘The present study aime to enhance the Whest_gronth by Aenpoary sepresnng nal meroflora The eftect of noeuaton on Venous eet varies ee evaluated. The sunvabity snd Dalferaton of wolates bacteria around foot surfacas reports, ‘The bene efect of using Mateus ar compementary owes to minetal Nertieaten. on gan yield) cher ompeation “ot whest euttvars Salina 8 and. Side? we ‘nventigatoa Materials and Methods Pathalogy Dept Fac. of Agie., Mansoura Unversity ding 1200012001 to inverigate the effect of appicaton of two Daterlzars end tives trent lvls of iui ero (2256 NI rite ang armions on the yea, chmiel sompesten yl components and NPK uptate an wheat eutvars She 8 ane ‘Scr Asceprium brasionse EKMISB war obtained fom iattute of Mirbleogy, Academy’ f Saence, Moscow USSR. Ao/hizobumt tauinocane G4 wae cbtanes rom infestans ies Research ‘growth of A. brasilanse wes provided by Nelsen and Knovdes 970) ane eauinodane wns etre in TGYE macium isdhs ra, 1889) Inoculation of grins and soit: The wheat s86c5 were inculated ‘wn Azoopntim braienae (AZ| ang Azorsrobum cavinodane TR, arabe gum ston [| was uses axa slang apart hon hese sence were inculsed with Azospiiium Brecionse Ce ‘Azehizobium eeulnodane Approx. 10" fot) mi" was added {rae teach pot frat plating so the second wes ater one ‘woek The. total bactonal counts in the hsoaphere Dore on young root. the ston wise sey aut, pote Sn sornsalit agar of ed mppimentes with 0.05% yatta, ‘umber technique ae descrbed by Siskandargsh et a (10931 ‘Themtvagencartent lysis iKjadal vas used to deletrine the {otal N content IBaten ata. 1899) ‘The sol physieal sn charcel analyi of he tecomevanded N lvel (76 United) were eppted. The green house exparments were conducted pots containg 20 Kg ot Phosphate farther vas apped az super phosphate [16.69% P.O.) @ te ngted duing preperation, ‘Sol machancl malyie was done accering to Per (1878, pH EC dvalasieP aby Jackeon (1067). M.avalleN and Kan the avalable Fe, Mn, Zn and Cu by the methods of Unasay and Nowell 11878) ‘Oven ded plant materi (rains and straw vas used to ‘determine WP snd F conceneations in the digestive exist 3t HisO. + HCI (11) according to Peterburgsh 11068) perimental ete 1181BLHawary of al: Etfect of biofertiizer on wheat yield and soll biology "ale Sal palea and ene! othe sb ue il 2 Mumbo vite Arshad tne of cel see ce yn een OT eisane -g 9000-1 T) side 1 Foon a fon A § 10004 1000 Bes 3 fe 6 a3 ae ‘Treatments Fig. 1: Dey wiht (pol of te two whest vrei gien vill a affected by boterlzer Pot experiments (95 pets pe experiment) were conducted in & Tandomaea complete Seok design (12 pote per block) in for Tepleser for to wheat genotypes. The data ware stxirtcahy ‘alan arording Yo CoSTAT Program (1980) using LSD at 5% Reoulte and Discussion Ettect of mirobal Inooulein on the viable umber of ‘Azospiinen and Asorcobhe in zoeper sll The oganiems {na 10 wreaks of pron on yeast entroct peptone adm of ‘Assen ane Azomhaabium eutures ot yeas ert man ‘Spar (Daboolaere of 1208) TO weeks! Concerning the effect of inoculation treatments on the Bacterial oount observed betreen the number of bects uing Noni dung the course of erperment Table 2 jon, nitrogen aplication rate ad thie isl (glpot Recute of weight responce to mites Inoeulston, the ntiogan applications snd ther interactions on fan yd gps) g 1) showed tat inaction of heat needa Sih “Asombeblum, Azospnium and mitre of micrba 537.1 and 42.5% 1m Sie | vanity respective so compared wath contol, ‘The sppcalion of nitrogen alone ft 6 and 100% increased! the gn yi by 7.7 and 753%in Saka 8 and 28-2 ana Ge 7% In Sionicandy andthe adie of 10D Nt. Azcricoiun! gave ‘hetignest gran vols 92 Tin Saha and 96 1%in Sie. TM 1 signtcant nsease may be due tothe production of youth evelopment and morphology ot he heat root Arlt {1998}, Dobbelnwe tat (1999) and Hammens ta (2001) ‘omar (1896) found that grin yi vine neessed by inoculation ‘oth brosnee ard war sigan higher with 26 than 8 Kg Nomar ata (1996) aio found thatthe grain Weld and pote inaoulon ofthe varaty Sn 88. Sram yd (eth The vein of what. sads. ith increased stra ald of vient Te increnne wae 63.1, 28,8 a0 in the variety Side’ 1 respectively 86 compared with the control ecnbuted to the erence In stew Vis (46.7 snd TBS% In Seine ana 2613 and 87.4% Sie 1 rospectiveiy) his the {hehighest ston yiddin Sane 8 (82.0%) and in Si 1 (08.2 ur resulta aren seco wth cased ef a (1896) who found ‘hat sings mitre of Azetobsetorehreacoecum Asoepniurn poten applieton to wheat ev, Sata 86 doubled the fn ErDin apa Anerapsu (1005) found that gfan and saw Yas ineraced with Ineraatng ree ot N fers and sae inoculation ‘th Aaogpitan sp. gave beter results than zatebacter 9. 1000.grin weight: The 1000-gaine wight f the two wheat Vlaies wre affected by mitoblal Ineulon /Azoriobli Sea 100%) sna tha inersctons (Pe. 3h Marobl! nowltion of eda th [Azorzebum and Asopiilum Sod mix} of rir Incclatin aignieasy increased 1000 rane welHt. Ths increase wis 106,86 and 1116 nGatha, 182BLHawary of al: Etfect of biofertiizer on wheat yield and soll biology Fig. 2 Dry weight (etl o 1 =O> Seine es-| ~O- Si 2 i] ee ue # so. 3 ° ee ‘Treatments Fig, Want of 1000-gran as attcte by blcetla spp n 0 Erect of microbial iocuaton,spplistion rate ot nitrogen and ‘hal era vrELHawary of al. Effect of biofertiizer on wheat yield and eoll biology ‘Mee iy blair root ‘ion untae of Sone 8 Gran otte of Sl Weow raz ito O12 067 00 Ott O88 “TWbie HO Uptake Ippon) of whet vay wm fected By Notas nat Swine 8 saw opto 2 ost oto a2 80817 “Fi Vienssbum | AZ cons MK eaheobom + Assis Uptake in tra: NPA uptake wens significantly ieraned ina Weatmante for Sethe 8 and Ss 1 varieties apna the contrat {Tavle ‘hes inetease in NPICuptae in beth west Votes twee followed by 100% + Azosnmnium wahout signee aiferencs, Fyne and Vises (19521 ar Zaha fat 11800, fous st Dine NPC cancanratins were higher with A braslense 8 kg Elser ota 1807) conducted ea experiment at Wadi, ‘South Sina, ung ofganie manures sone sng in omeination vith thee levels of N chunsca Terisar "The waraty Saha ® soem conesponding improvements in mictbla count, Azotobacter, ‘Azospityand phosphate dsoling bacon, a wll 30 NPI ptake ich ete rtecta on figh gain sn Sta i itis concludes that inaction wth Asohizoim reseed the Yield of wheat growin pots with high levels of Bald organs ‘itogen ‘erlzer "These rebut showed the. possiisty ot Inoculiien of Azriobiam in comblnation with nivonen Teer A improve the yd of wihentexpeily the varity Sabha 8 ‘ay be due to enhancing the development of fot In the ey [towth stage, whien may be one ofthe factors respenable for {he weld teveasen, those reste ein conferity wah oe ‘bianed by Bhatt and Hess (7898), Webster a! (18E8) and Selman a 11990) Finaly we an recommend that half of the soled N frizer ‘aul be saved west seeds we nacusted vith nan-symblatc fing bacterin erpecly earhzabum nathout affecting te ys Furthermore, the id N fericre crignsted orm organi {ourest wore much sttective and te varety Saha ® was ors References Aly, 555. SM. Seiman, EA Gael and ME Aint, 1998 ‘Sigiteans of tee NZthing bacteria and itiieation ahibors en coving the appued agen to whet plans Gul Fe. Ai Uni car pp 347-368, ‘of wheat roots by an exopolysaccharide, Appl Env. Microbiol. aman, ¥. 1588, Sigiteance of ting sd level of inoculation "oth iouphete bacteria cn wheat plant. Se Bll Biochem ie: 267-901, Batten, OD. AB Blakeney, SM. Geneon, RJ. Henry, AC. ‘MeCattery. PE acon and OP Heenan. 1891. Rapid Geterminaton ot shoot niogin status in ee using nese Iniared retectance spectioccopy. J Sc Fa. Agi. I 17 ‘attra T. and ©. Hees, 1980. 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Evaluston of ntogen fied by Azoepniirn Tatlance NOSO (Cerin in too nce culvate using NA {lution tachrigue. nn Agi. Se (Cate, BSE. ‘omar MIA, MH Hagazy,R AA Ebasi, MSA, Soiman anc TMM. Sobh, 1991, Etect of mocdaton with ézcbactra on dof west undr greed lava of nitrogen fertilaion rn barat Se caro, 99-108, 1188ELHawary of al. Effect of biofertiizer on wheat yield and eoll biology Evatustng the efficiency of Incelatn some elaztophs on void ana protein coment of 3 wheat covers under graded lave ofritrogentertsaton. Ann Agel Sei (Cael, 679.50, fougth, AL, 1968. HandBock of Agronemie Chemisty Kelep Punishing House, Moscow, ln Russin | pp: 208, Pinar, €5,, 1974 Sol and Plant Analyse, Un Adela Enter ‘Slane Publier nc, Neve York eyndes, Land Vacsah 1802, Use of Azopitm brarlonce {ae boferizer in intensive wheat cropping. Pant and Sa. 68 ‘abr SRS, S.A Saleh and LAA, Melinemar, 1898. 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