Prepare 2 solution for

each gum solutions Prepare 50 mL 1.5%

gum solutions of pH 3 & Weigh 25 grams of
7 clean and dry seaweed

Stir for 10 mins or until

completely dispersed Treat seaweeds with 1
liter 5% NaOH solution
in a 90C water bath for
30 minutes
Compare their properties
and behavior at room
temperature, heated,
Wash with running water
boiled, cooled, frozen and
thawed.

Soak sample in 750 mL

0.5% HOAc for 30 minutes

Wash with running water

Extract sample in 1 liter

boiling water for 1 hour

Blend and filter

through layers of

Freeze the filtrate overnight

Thaw the gel the following

Filter the thawed gel

using a cheesecloth

Weigh the dried

sample and calculate
Add 100 mL CO2-free
Weigh the
0.5percentage
g yield
Weigh 200 mL pectin water, 1 g NaCl, 6 drops
beaker of phenol red indicator
Prepare 1% agar solution solution
by adding 1 g agar
 Titrate the solution with std 0.1 N
Dilute to 100 g with distilled
NaOH until the fuchsia endpoint

Heat the mixture with

agitation until completely Transfer titrated solution to a flask
dissolved
Mix 25 mL 0.25 N NaOH and
Replenish any moisture lost stopper

Stand for 30 minutes at room temp

Divide the sample equally

into two 50-mL beakers Add 25 mL 0.25 N HCl, swirl

Allow to stand for 12 hrs Titrate with std. 0.1N NaOH to

the fuchsia endpoint

Measure gel strength

using a gel tester Calculate the methoxyl
content

Prepare buffer solutions Prepare 6 sets of 50 mL 2

adjusted to pH 3 and 7. % pectin solution: three for
pH 3 (Set A) and three for
pH 7 (Set B)

Bring rapidly to a boil

Continue slow boiling for 5

1 part sugar: 1 part solution for 1
Add sample in each set; part sugar: 1 part
solution for 1 sample in each set; and 1
part sugar: part solution for 1 sample
Boil briskly for 5 in each set
minutes

Cool to room temperature

Vigorously mixcompare
1 g Get samples of -
Observe and
sodium alginate in 100 cellulose and Na-
properties
mL distilled water carboxymethyl cellulose
 Add a drop of red food Observe differences in
color terms of various properties

Stand to eliminate air

Prepare a 0.5% calcium chloride

bath

Stand for 30 minutes at room temp

Prepare 200 mL of 0.1M HCl

Fill a syringe with sodium

alginate solution

Drip to the
calcium chloride

Add a few drops of the sodium

alginate solution into the HCl
solution.
Record what
happens
Collect the clumps and rinse
with water after 1-3 mins.

Pick out a clump and feel

the texture

Compare the 2 sets

prepared