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To cite this article: R. M. Saunders (1985): Rice bran: Composition and potential food uses, Food
Reviews International, 1:3, 465-495
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Food Reviews International, 1(3), 465-495 (1985-86)
R.M. SAUNDERS
Western Regional Research Center
United States Department of Agriculture
Albany, California
Abstract
465
INTRODUCTION
PERICARP
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PALEA
(HULL)
STARCHY ENDOSPERM
LEMMA
(HULL)
EMBRYO (GERM)
PERICARP SUBALEURONE
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RICE BRAN
Production and Composition
The term bran1 is generic. The final physical and chemical nature of bran de-
pends upon rice variety, treatment of the grain prior to milling, type of milling
system, degree of milling, and fractionation processes operative during milling.
Figures published on the composition of bran show a wide variation (Table 1),
which reflects the influence of these variables upon the final bran constituents.
Because of such variation it is standard market practice to specify limit
composition. For example, in the U.S. such limits for rice bran may be fat and
protein not less than 12%, ash not more than 17%, and fiber not more than
12%. This practice often leads to deliberate adulteration of clean bran with
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hulls as a profitable venture. The latter action would negate the use of such
bran for direct food use.
In developing countries and to a considerably lesser extent in developed
countries, rice is milled in one-stage (huller) mills that remove a single by-
product mixture of hulls, bran, and germ during the production of white rice.
Hand-pounding of rice as a means of milling also produces this same mixture of
hulls, bran, and germ. While it is difficult to assess how much of the world's
rice is milled by these one-stage operations, it is likely that 70-80% is milled
in this manner (5). It is unlikely that this rice-milling byproduct mixture of
bran and hulls would ever find use as a food source. Even if the mixture is
separated into coarse and fine fractions, little difference is noted in composi-
tion between the fractions (7). The apparently insurmountable problem of
removing hull fragments from bran has led investigators to regard utilization
of bran as a food source from hand-pounding or huller mills as impossible (8,
9). In this case, the revised potential for utilization of rice bran as currently
milled as a food source would realistically be in the order of 25% of the num-
bers noted earlier (Introduction), or about 8 MMT bran, containing 1.3 MMT
protein and 1.4 MMT oil.
However, the use of two-stage or multistage rice mills in which bran and hulls
are removed and recovered separately during the milling process to produce
white rice is growing. These milling operations first remove the hulls (or
husks), either by a disc husker or a rubber roll husker. The latter unit is the
preferred system in any new modernization of rice-milling operations. Prior
removal of the hulls provides a clean brown rice, which in subsequent debran-
ning (whitening) operations yields a bran with minimal hull contamination,
1
Unless specified otherwise, rice bran denotes bran from both raw and parboiled rice and contains
germ and polish. The author is indebted to his colleague De Irving for supplying Figures 1 and 2.
470 SAUNDERS
a>b
Table 1. Range in Composition of Rice Bran
Range Protein (%) Fat (%) Fiber (%) Ash (%) NFEC (%)
ing (or pearling) of brown rice produces bran. This bran is a fine powdery
material, made up of pericarp, aleurone, pulverized germ, and some endosperm
(white rice fragments). A detailed histological and histochemical study of the
caryopsis fragments in rice bran has been published (6). The amount of broken
rice in the bran depends on the rice variety and care taken during milling but is
primarily influenced by temperature and moisture conditions during the paddy
drying cycle (10,11). In most milling operations (>99%) the germ becomes
part of the bran stream, although in a few instances the germ is separated.
Whiteners, or removers of bran (and germ) from brown rice, are of two
types: the abrasion type, such as the cono-mills, and the friction type, such as
the polishers or jet pearlers. Proximate analysis of bran from the two types of
whiteners is shown in Table 2. In some instances calcium carbonate is added as
a milling aid; its presence modifies the resultant bran composition (6).
The yield and composition of bran produced in two-stage or multistage
milling varies with the degree of rice milling practiced. In general, paddy rice
Percent
Country Removed
India3'b 4-6
Indonesia8 4
Japan a ' c 7-9
Koreaa'c 7-8
Liberia 4-6
Pakistana 4
Perub 4-6
Philippines3 7
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Spain" 8
Sri Lankaa>b>c 4
Taiwana 7
Thailand a ' b ' c 7-9
U.S.A. 8-10
a
SeeRef. 13.
b
See Ref. 5.
c
See Ref. 14.
d
SeeRef. 12.
yields theoretically 25% hulls, 65% white rice, and 10% bran, which includes
germ and polish. These figures might be encountered in a sophisticated com-
mercial enterprise. However, in practice the degree of milling (or the amount
of bran removed from the brown rice kernel) varies considerably (Table 3).
As a consequence, the yield and amount of germ and/or polish in the bran
varies, as does the bran composition. The extent of variation in bran composi-
tion as a function of the degree of milling is apparent in Table 4. A low degree
of milling (undermilling) is practiced usually for economic reasons; less bran
removal, with low market value, means more rice, with high market value. A
high degree of milling tends to be practiced where rice is intended for export
or enters marketing channels in which there is a requirement for considerable
shelf storage time. Undermilled rice is less stable than well-milled rice because
of the bran residues left on the kernel. The consumer of rice does, however,
benefit from undermilling, since vitamins, protein, lipids, and trace minerals
are left in the bran fragments still adhering to the kernel.
In those limited instances where polish is separately collected during milling
of the rice kernel, the polish composition would approximate (dry basis):
protein, 14%; fat, 14%; fiber, 3%; and ash, 8% (2,16). Polish is that fraction of
472 SAUNDERS
Table 4. Variation in Rice Bran Composition as a Function of the Degree of Rice Millinga
Bran Composition
the kernel where the endosperm and pericarp are joined together. Thus, polish
would be relatively rich in aleurone and subaleurone material.
In general, bran from parboiled rice contains substantially more oil than does
bran from raw rice. Raghavendra Rao et al. (17) report oil content of par-
boiled rice bran to range from 28.2-34.2% compared to 24.2-25.9% for raw
bran at up to 5% degree of milling, although these oil contents are abnormally
high in either case. Benedito de Barber et al. (18) reported an oil content in raw
bran of 19.9% versus 23.1% for the parboiled bran at 4% degree of milling. At
4-9% degree of milling, these oil levels were 12.9% (raw bran) and 13.9% (par-
boiled bran). Migration of fat from the aleurone cells to the outer bran layers
during parboiling, while difficult to perceive, is generally accepted as the main
contributor to the phenomenon (19). Bran from parboiled rice contains less
starch and thus less nitrogen-free extract, but more fiber, ash, and protein.
Less endosperm fragments (starch) show up on the bran because parboiled rice
suffers less breakage during mUling.
It must be emphasized that in the following section on rice bran compo-
nents, the term bran means the bran stream produced in a two- or multistage
rice mill. This bran would be expected to contain the germ.
Protein
Protein distribution within the dehulled rice kernel ranges approximately: bran
(including germ and polish), 17-30% (16) and milled rice, 70-83% (16). This
distribution varies with the degree of milling, and to a lesser extent with the
variety and protein content of the grain. The distribution of protein solubility
fractions in bran has been reported (20) to be albumin, 37%, globulin, 36%,
prolamin, 5%, and glutelin, 22%. Values for albumin, 40%, globulin, 21%,
prolamin, 3%, and glutelin, 36% were found elsewhere (21). Resolution of
RICE BRAN: COMPOSITION AND USES 473
albumins and globulins and their molecular weights have been reported (22,
23).
The major soluble basic proteins of bran have been described as cytochrome
C and a blue, copper-containing glycoprotein (24,25).
Nonprotein nitrogen accounts for approximately 16% of the total nitrogen
of rice bran (26). Major free amino acids are glutamic acid, alanine, and serine
(27).
Rice bran contains numerous enzymes, some of which are likely to be at
least partly of microbial origin. Akazawa (28) has compiled a list of these
enzymes. The enzyme lipase is the most responsible factor in the nonutiliza-
tion of rice bran as a foodstuff (see Rice Bran Stabilization section).
The protein (N X 5.95) content of rice bran usually ranges between 9 and
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17%, although it can be closer to 20% in defatted bran. Protein content is influ-
enced by variety, environment, and nitrogen fertilization.
The amino acid composition of rice bran protein is listed in Table 5.
Carbohydrates
Standard
Amino Acid Average Deviation
Lipids
Bran typically contains 10-23% oil, although the value may exceed 23% in
undermilled, parboiled bran. Three major fatty acids, palmitic, oleic, and
linoleic, make up about 90% of the total fatty acids (Table 6). Rice bran oil
is close in composition to sesame, corn, cottonseed, and peanut oils.
Bran lipids are classified into the groups glycerolipids, sterol lipids, and
sphingolipids. The glycerolipids comprise glycerides (89%), glycolipids (8%),
and phospholipids (2%) (43). The glycolipids include predominantly mono-
and diglycosyldiglycerides, the sugars of which are galactose and glucose.
Phosphatidyl-choline, -ethanolamine, and -inositol comprise the phospho-
lipids (44). In the sterol lipid group, free sterols, sterylglycosides, and acyl-
sterylglycosides are present (45). Ceramides and glycosylceramide make up
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most of the sphingolipid class (45). Cholesteryl esters and cholesterol have been
identified in rice lipids (46). A potent antioxidant, oryzanol, is reportedly
present at 1 - 3 % in bran lipids (47).
Rice bran oil contains wax (2-5%). The major wax acids are behenic, cerotic,
isocerotic, and lignoceric, whereas the alcohols include ceryl, isoceryl,
montanyl, and myricyl (42).
Lipids in milled bran are rapidly hydrolyzed by the action of lipases. This
subject will be discussed in detail in the section on Rice Bran Stabilization and
Recovery of Edible Oil.
Comprehensive reviews are available on rice lipids (44,48,49).
Composition (%)
Myristic(C14:0) 0.4-1.0 tr
Palmitic (Cl 6:0) 12-16 15.9
Palmitoleic(C16:l) 0.2-0.6
Stearic(C18:0) 1-3 1.7
Oleic (C18:l) 40-60 40.7
Linoleic (Cl8:2) 29-42 37.9
Linolenic(C18:3) 0.5-1.0 1.4
Arachidic (C20:0) 0 0.6
a
SeeRefs. 39,40,41,42.
476 SAUNDERS
Vitamins
Vitamins found in rice bran have been tabulated by Juliano (16). Ranges for
these values are listed in Table 7.
Variation in vitamin content undoubtedly reflects analytical methodology,
rice variety, degree of milling, and hull contamination. However, it is clear that
all vitamins are concentrated in the outer kernel layers, principally in the
aleurone layer and scutellum. By calculation, the bran-germ-polish component
contains 78% of the rice kernel thiamine, 47% of the riboflavin, and 67% of
the niacin. It has been reported (50) that rice milling results in the loss to
human consumption of 76% thiamine, 57% riboflavin, and 63% niacin. Similar
losses would occur in all other vitamins because of their concentration in the
bran and germ.
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Minerals
Juliano (16) has tabulated mineral analyses for rice bran, the ranges for which
are listed in Table 8. Minerals are concentrated in the kernel bran layers. The
bran-germ-polish fraction contains about three times the mineral content of
milled white rice. Phosphorus is the major mineral, about 90% of which is
Content
Vitamins (ppm, dry basis)
Vitamin A 4.2
Thiamine 10.1-27.9
Riboflavin 1.7-3.4
Niacin 236-590
Pyridoxine 10.3-32.1
Pantothenic acid 27.7-71.3
Biotin 0.16-0.60
Myoinositol 4,627-9,270
Choline 1,279-1,700
p-Aminobenzoic acid 0.75
Folic acid 0.5-1.46
Vitamin B i2 0.005
Vitamin E (tocopherols) 149.2
a
SeeRef. 16.
RICE BRAN: COMPOSITION AND USES 477
Aluminum 53-369
Calcium 140-1310
Chlorine 510-970
Iron 190-530
Magnesium 8,650-12,300
Manganese 110-877
Phosphorus 14,800-28,680
Potassium 13,650-23,960
Silicon 1,700-16,300
Sodium 0-290
Zinc 80
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a
Adapted from Ref. 16.
Considerable research has been reported on conversion of rice brans into sub-
fractions which may contain more of the desirable nutrients and less of the
undesirable components. Protein has been generally used as an index of positive
enrichment, which in some cases has led to the term "protein concentrates"
being applied to some bran subfractions. Fiber has generally been considered
an index of undesirable components, although this has been modified to some
extent in recent years because of the purported desirability of dietary fiber.
Fractionation processes include specialized dry milling of raw or parboiled
brown rice, air-classification of rice brans, and aqueous extraction of brans at
ambient or alkaline pH followed by recovery of fractions differing in their
solubility or emulsification properties. None of these processes has been
adopted by commerce to any great extent.
478 SAUNDERS
Dry Fractionation
ground defatted rice bran (10.6% protein; fiber 11.4%) provided a high protein
(12.3%) fraction in a 48% yield, containing 6.9% fiber (53).
Through pin-milling and air-classification, defatted rice bran of original pro-
tein and fiber contents of 19.4% and 8.2%, respectively, was converted into
subfractions containing protein as high as 23.5% and fiber as low as 1.9% in
50% yield (Table 9). Unfortunately, the ash content of the protein-enriched
subfractions is probably too high for direct food application.
Fine grinding and air-classification of full-fat rice bran did not provide frac-
tions of composition different from that of the starting bran (53,54).
Wet Fractionation
Wet grinding of full-fat rice bran using disk, rotating, or colloidal mills fol-
lowed by wet-particle size classification using meshes or hydrocyclones yielded
three fractions: (1) a high-fiber fraction, (2) a low-fiber fraction, and (3) an
aqueous concentrate of vitamins and minerals (Table 10) (6). Unfortunately
this process suffers from the associated high drying costs.
Protein concentrates of various compositions have been prepared from full-
fat and defatted rice bran by an alkaline extraction technique similar to the
process of preparing protein isolates from soybeans. Solubilization of nitrogen
in rice bran as a function of pH has been investigated (55,56). Earlier, condi-
tions had been recommended for alkaline extraction of rice brans, namely pH 9
at room temperature for 15 min (57). This process has been patented (58).
These conditions were deemed appropriate for economic purposes and for the
avoidance of negative amino acid-alkali reaction products. Screening of the
aqueous alkaline mixture to remove particulate (fiber) material provides an
aqueous phase containing a major portion of the original bran protein, lipid,
and starch. A protein-lipid-starch concentrate could be obtained from the
aqueous phase by heat or acid precipitation (Table 11). Removal of starch prior
to protein precipitation altered the protein composition as shown in Table 11.
Lipid extraction from these protein concentrates would increase the final pro-
tein level.
The composition of the fatty acids in these subfractions averaged oleic,
48.9%, linoleic, 37.0%, palmitic, 14.1%, and traces of steric and linolenic,
similar to the rice bran pattern (Table 6) (57). The amino acid patterns of
these subfractions and those of the bran from which they were prepared are
listed in Table 12. Lysine is no longer the first limiting amino acid in the sub-
fractions. The subfractions are enriched in iron (57).
To decrease water required for processing (and hence drying costs), this
process was refined to provide a liquids recycle operation (57,58). A formula,
y = x (1.97n + 4.35) was determined, where y equals the weight of aqueous
alkali, x equals the weight of rice bran, and n equals the recycle batch number.
In practice, the solids content of the recycling liquid phase leveled off at cycle
Table 11. Yield and Composition of Fractions Obtained from Rice Bran by
Alkaline Extraction8
Rice bran
Heat 20.5 22.8 32.7 0.7 11.7 22.9
Heat, starch removed 12.2 33.3 49.2 0.5 4.5 1.3
Acid 16.8 28.2 41.7 1.0 5.2 20.4
Acid, starch removed 12.3 35.8 50.8 0.6 3.3 1.5
Defatted rice bran
Heat 20.5 33.7 8.2 1.6 17.0 25.5
Heat, starch removed 9.3 58.2 16.2 1.0 6.1 3.2
Acid 16.8 43.0 12.2 1.6 5.7 29.7
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Table 12. Amino Acid Content of Rice Bran and Subfractions Prepared
by Extraction of Rice Bran at pH 9 a ' b
Bran Subtraction
Table 13. Yield and Compositions of Fractions Prepared from Rice Bran by
Extraction with Sweet or Acid Wheya
Sweet whey
starch present 23.1 23.3 31.0 2.0 8.6
starch absent 8.5 47.8 46.4 0.1 1.5
Acid whey
starch present 21.8 34.0 24.0 3.2 11.5
starch absent 6.7 54.1 32.1 0.4 3.2
a
Adapted from Ref. 61.
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5, at 6.5% solids, half of which was sucrose, glucose, fructose, and raffinose
(57).
Subfractions from bran and defatted bran also have been prepared via
alkaline extraction by Chen and Houston (59) and Lynn (60), respectively.
Yield and composition were similar to those products described in detail above.
Whey adjusted to alkaline pH has been used to extract rice bran as a means
of deriving subfractions enriched in protein (61-63). Typical yields and com-
positions are reported in Table 13 for these materials with and without starch
removal during the extraction procedure.
Several enzymes have been tested in an effort to improve extractability of
protein from rice bran. Amylases showed some improvement, whereas cellu-
lases, proteases, and pentosanases did not (60,64). Use of hydrogen peroxide
(65) or fine grinding (55) did not improve protein extractability from rice
bran, although fine grinding did improve extractability from purified rice germ
(55).
Mitsuda et al. (66) report high-protein subfractions recoverable from rice
bran by extraction with sodium dodecyl sulfate, ammonium hydroxide, sodium
hydroxide, sodium chloride, or urea. Yields ranged from 14 to 30%. Alcohol
extraction of the material extracted with sodium hydroxide yielded a protein
isolate, 94-99% protein. Incorporation of an anion-exchange resin in the
extraction medium improved protein recovery.
Another process to wet-fractionate rice bran has been described by Mihara
(67). Bran is first ground in water, then separated into aqueous and solid phases
by centrifugation. From the aqueous phase, a protein-oil complex is removed
by chemical means, then deoiled to produce a protein concentrate and oil.
Phytin is recovered by precipitation, leaving a vitamin-enriched aqueous phase.
Starch is further fractionated from the solid phase by resuspension and particle-
size classification. The yield and composition of subfractions are listed in
482 SAUNDERS
Table 14. This process is complicated, requiring substantial drying, and is thus
not likely to be economic.
Rice bran disintegrated in hexane with a high-speed blender was sieved through
60-mesh screens. The fraction passing through the 250-mesh screen, after
hexane removal, yielded (35-40%) a product containing 22% protein, 50%
carbohydrate (80% starch), 4% fiber, and 20% ash, dry basis (68).
The utilization of rice bran as a food is limited to some extent by its fiber
content, but the major obstacle is its instability. Enzymes, both natural to the
bran and of microbial origin, are the major cause of bran deterioration. Lipases,
and to a much lesser extent oxidases, are responsible for these deteriorative
changes by promoting hydrolysis of the bran oil into glycerol and free fatty
acids (FFA). Hydrolysis and oxidative rancidity are associated with this de-
terioration. The consumer experiences bitterness and a soapy taste (6). Within
the intact rice kernel, lipases are localized in the testa-cross layer region, while
the oil is localized in the aleurone and germ (69). Thus, the substrate and
enzyme are compartmentalized. However, once these regions are disrupted
during the milling process, enzymatic hydrolysis is possible and proceeds
rapidly. Microorganisms present on the surface of the kernel would also then
have access to the bran oil (70).
One process to avoid this problem has been commercialized, namely the
X-M Process, in which rice is milled under hexane (71). In this manner, the
RICE BRAN: COMPOSITION AND USES 483
oil is separated from the bran during the milling operation to produce an oil
which can be directly refined for edible purposes. The bran from this operation
can then be desolventized (via heat) to provide defatted rice bran, which in
fact has some food applications. Because of poor economics, the X-M Process
is no longer in operation.
Thus, throughout the world, lipase and bran oil do come into immediate
contact once the rice is milled. The rate of FFA release is very high and is
affected strongly by environmental temperature and moisture. FFA release of
5-7% in a single day or up to 70% in a month has been widely recorded. As
FFAs increase, the refining loss for potential edible oil production increases
more rapidly since losses during refining are two to three times the FFA
percentage (9). Rice bran oil normally contains 1.5-2% FFA at the time of
milling; less than 5% FFA is desirable in the crude oil for economic recovery
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Stabilization Processes
Dry-Heat Methods
Open-pan roasting (batch) 84
Stationary bed (batch) 85
Steam-jacketed conveyor (continuous) 86
Flu-gas-jacketed screw conveyor (continuous) 87
Oil-jacketed screw conveyor (continuous) 88
Fluidized bed (batch) 89,90
added heat to drive off some of the moisture as pressure is released appear
effective and efficient.
Extrusion cooking with low-cost extruders, which run on electricity and in
which heat is generated through friction with no steam or drying requirement,
appear most appropriate (72). Preliminary economic analysis indicated that the
use of such an extrusion cooker in rice-milling operations to stabilize bran for
edible oil recovery would be financially feasible (9). This prediction appears
to have been borne out in practice (73).
Only in one case has a thorough economic analysis been made of the sta-
bilization system, namely the Brady2 extruder system (9). The simplicity of
this unit, its low cost,3 and effective stabilization at high-quality continuous
throughput seem to predicate its eventual use in developed and third world
countries, albeit only at multistage rice mills.
2
Reference to a company and/or product named is only for purposes of information and does not
imply approval or recommendation of the product to the exclusion of others which may also be suitable.
3
U.S. $15,000 in 1985.
RICE BRAN: COMPOSITION AND USES 485
FFA (%) 4 51 3 4
H2O (%) 11 12 7 10-11
Bacteria (X 102/g) 82,000 7,400 9 4
Barber and Benedito de Barber (12) point out that research and development
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work on rice bran stabilization had never gone beyond the heat-stabilization
stage. Recent work on stabilization using the Brady extruder has addressed
poststabilization consequences, including handling, storage stability, oil extrac-
tion and refining, chick and pig feeding, and incorporation of stabilized brans
into foods. These are described in some detail below.
In practice, it has been recommended that extrusion at 130C, followed by
holding the bran for 3 min at 97-99C prior to cooling, effectively stabilizes
the bran (73,91). In this process, a temperature of 130C is attained entirely
through friction, and the bran is at this temperature only a few seconds. Sim-
ilar conditions were noted in another extruder system (74). In addition to
destruction of lipase activity, peroxidase activity was also destroyed under
these extrusion conditions. Long-term storage studies (Table 16) indicated
that stability against FFA development persisted for at least four months,
contrary to most processes using dry heat to stabilize (12). Extrusion-
stabilized bran was essentially sterile (Table 16). At these stabilization con-
ditions, electrical energy requirement was 0.076 kwh/kg (73). Extrusion-
stabilized rice bran contained 6-7% moisture and was in the form of small
flakes, with 88% larger than 0.7 mm in diameter. This material could be ex-
tracted with hexane to recover oil without further pelleting (92), in contrast
to most other stabilized brans, which require agglomeration or pelleting prior
to oil extraction (12). The crude oil was refinable using conventional (40)
winterizing, dewaxing, neutralization, bleaching, and deodorization (92).
The refined oil compared favorably in color and flavor with other vegetable
oils. It contained 170 mg/kg of tocopherols (Vitamin E) and would thus be
expected to be stable. Fatty acid composition of refined oil is shown in
Table 6 (under Typical).
Stabilized rice bran and extracted stabilized bran in which oil was added
back in the test showed a 20% improvement in feed efficiency compared to
unstabilized bran when fed to chicks at 60% of the ration (93). Stabilized bran
486 SAUNDERS
was marginally inferior to unstabilized bran when fed at high levels (20-40%)
to pigs (94). Elsewhere, Tortosa et al. (95) had shown a slight improvement in
pig performance when fed rice bran stabilized by another procedure (79) when
compared to the unstabilized bran.
Stabilized and hexane-extracted stabilized rice bran from the Brady ex-
trusion system have been tested in food systems (96,97) (see section on Food
Uses).
The prime reason for stabilizing rice bran is to convert this resource into one
providing edible oil. Edible rice bran oil at present is obtained in Japan and
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India only in those cases where the bran can be extracted within a short time
after milling prior to FFA buildup. In these cases the bran is steam-agglom-
erated (Japan) or pelleted (India) prior to hexane extraction. Oil-refining to a
high degree is practiced in Japan using either alkali-refining or physical-refining.
Cost-effective stabilization technology using extrusion is now available on a
scale suitable for small and large two-stage or multistage rice-milling opera-
tions. Stabilization at single-stage rice mills is considered impossible due to the
poor economics in extracting rice bran containing hulls and thus having low oil
content. The extrusion process has the advantage of not requiring additional
pelleting prior to extraction, and in fact the cost of stabilizing is probably
similar to the cost of pelleting. Extrusion conditions have a positive nutritional
effect upon the bran as far as poultry feeding is concerned, and no negative
effects upon swine feeding. Most importantly, the stabilized bran can be stored
for long periods prior to extraction without loss of oil quality (92).
Since the economics appear favorable (9), one can expect that production
of edible rice bran oil will increase in the near future through implementation
of low-cost extrusion technology.
The general nutritive value of rice bran has been well established in composi-
tional data and pertinent references listed elsewhere in this chapter. Actual
studies on biological value using test animals is severely limited. Some idea
of energy value and nutrient digestibility as a feed is evident in data listed in
Table 17, although bran compositions are not defined and these values are
likely to be low compared with those encountered in a good quality bran. If
one assumes typical starch and sugar contents to be 10% and 5%, respectively,
RICE BRAN: COMPOSITION AND USES 487
the caloric content of the representative friction-milled rice bran (Table 2) cal-
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a
Six subfractions differing in method of preparation and/or drying,
prepared from the same rice bran.
"Three subfractions differing in method of preparation and/or drying
prepared from the same defatted bran.
subfractions prepared from bran. These PER values are extraordinarily high for
a material derived solely from a cereal source.
Bran stabilized by the Spanish procedure (Table 15) (79) showed a PER
value in rats of 1.59 compared to 1.66 for the unstabilized bran (21). These
values were not statistically different, and nitrogen digestibility was the same
(73%) for both brans. In another study on these same materials, stabilization
resulted in a small though significant improvement in nitrogen balance but no
difference in PER values in rats (106).
Bran stabilized by extrusion (Table 15) (73) showed a 20% improvement in
feed efficiency compared to unstabilized bran when fed to chicks at 60% of
the diet (93), although it was slightly inferior to unstabilized bran when fed
to pigs (94).
In those feeding tests using stabilized bran, no adverse effects upon nutritive
value through the heating process were observed. On the contrary, antinutritive
factors were destroyed (see next section).
Bioavailability of nutrients other than protein from rice bran has not been
studied to any extent. Maymone et al. (100) report digestibility by sheep of
lipids, crude fiber, and organic matter to be 79.5%, 31.2%, and 59.5%, respec-
tively. Crampton and Harris (98) list total digestible nutrients to be 60%
(cattle), 73% (sheep), and 74% (swine).
RICE BRAN: COMPOSITION AND USES 489
Antinutritive Factors
Enzyme inhibitors noted in rice bran include trypsin inhibitor (107) and pepsin
inhibitor (66,104). The pepsin inhibition is believed to be caused by phytate
in the bran (104). The trypsin inhibitor is proteinaceous (107). Alpha-amylase
inhibitors like those found in wheat are absent (108).
Other potentially deleterious components in rice bran include hemagglutinin
(109), an antithiamin factor (110), and estrogenic activity (111). Trypsin
inhibitor and hemagglutinin activities were destroyed by heat during the
stabilization process (112,113). Chicks fed unstabilized raw bran had a sig-
nificantly heavier pancreas than chicks fed stabilized bran (93), though no
difference was noted in pancreatic weight between rats fed stabilized or un-
stabilized bran (21). Growth depression in chicks fed raw (unstabilized) bran
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has been noted frequently (93,114) and occasionally in pigs (6,94). Heat
treatment invariably improves feed performance in chicks, even though residual
trypsin inhibitor activity may be present (114).
Phosphorus is the most abundant mineral in rice (Table 8). According to
McCall et al. (115), phosphorus occurs as phytic acid (89.9%), nucleic acid
(4.4%), inorganic phosphate (2.5%), carbohydrate (2.3%), and phospholipid
(1%). Phytic acid, myoinositol hexaphosphate, occurs entirely in the bran
(116) and is associated with the aleurone layer. Bran obtained from 4% degree
of milling had phytic acid content of about 3%, 23 times the level found in
whole brown rice (116). The particles isolated from aleurone by Tanaka et al.
(51) contained 9.4% myoinositol and 11.6% phosphorus. Aleurone particles
described by Ogawa et al. (117) contained 67.2% phytic acid, 18.9% potassium,
and 10.8% magnesium.
There is controversy concerning phytic acid and nutrient availability. In vitro
observations suggest it is a protease inhibitor, but whether this plays a role
in vivo needs to be substantiated. Far more prevalent in the literature is the
question of the influence of phytic acid upon mineral availability (118).
Phytates have been considered to prevent absorption of mineral elements,
particularly divalent elements, and there are numerous studies to support this
claim. However, Morris and Ellis (119) reported iron in monoferric phytate to
be totally available to rats, and Graf and Eaton (120) report that phytic acid
had no substantial effect upon absorption of calcium or iron in mice.
Dietary Fiber
Only one study has been carried out on the dietary fiber content of rice bran
(121). The results are listed in Table 19. From these limited data, a regression
490 SAUNDERS
Dietary Fiber3
equation was derived in which in vivo dietary fiber (y) is related to crude fiber
(x): y = 3.34x + 5.69 (r2 = 0.931).
FOOD USES
Rice bran finds extremely limited use in foods, primarily because of the asso-
ciated oil deterioration problem (see section on Rice Bran Stabilization) and
hull contamination. Rice polish, though only manufactured in a few locations,
finds use in baby foods. Defatted bran use also is extremely limited, but a wide
variety of products exist,4 including specialty breads, a carrier for artificial
spices, a protein supplement and binder ingredient for meat and sausage
products, a raw material for production of hydrolyzed vegetable proteins, a
breakfast cereal and snack food ingredient, a tableting excipient, a source of
inositol, and an ingredient in pickles (6).
Stabilized rice bran finds limited use in rice flours and breakfast cereals in
the United States.
Numerous potential food uses for full-fat and defatted rice brans have been
well proven in laboratory tests but not yet in commercial practice. These
4
The author is indebted to Dr. J. Hunnell of Riviana Foods, Houston, for providing some of this
information.
RICE BRAN: COMPOSITION AND USES 491
include bread, muffins, pancakes, cookies, pies, and cakes using raw or
stabilized full-fat bran (60,96,97). Protein concentrates from bran have been
extensively studied as ingredients in breads, beverages, pasta, and confections
(21,60). Levels of up to 10% tended to give satisfactory products from an
organoleptic viewpoint. Nutritionally, protein content was invariably increased.
Oil extracted from bran in Japan is refined and used as an edible oil.
Estimates on actual tonnage vary widely, though 10,000 MT per year seems
reasonable. In India, edible oil production from rice bran seems to vary widely
from year to year, with not less than 5000 MT per year being produced.
RICE GERM
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Only in rare instances is rice germ separated from bran during the rice-milling
operation. This is the case in Spain and Egypt, where the germ commands a
premium price as a feed. It is believed that a very small quantity of purified
germ finds specialty food use in Japan. The merits of using rice germ as a food-
stuff have been thoroughly documented (6,123). Germ is rich in protein
(~25%), oil (~30%), and vitamins and trace minerals. It would seem wise to
devise and install germ separators for modern rice-milling operations; the
nutritional and economic returns appear to warrant such a move.
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