Name: vanessa

Exercise 2: Skeletal Muscle Physiology: Activity 4: Tetanus in Isolated Skeletal Muscle Lab Report

Pre-lab Quiz Results

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Experiment Results
Predict Question:
Predict Question: As the stimulus frequency increases further, what will happen to the muscle tension and twitch
appearance with each successive stimulus? Will there be a limit to this response?
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Stop & Think Questions:

What begins to happen at around 80 msec?
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Correct answer: c. unfused tetanus develops

How does the trace at 130 stimuli/sec compare with the trace at 50 stimuli/sec?
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Correct answer: b. Fused tetanus develops at this greater stimulus frequency.

How do the traces with 146150 stimuli per second compare with the trace at 130 stimuli per second?
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Correct answer: d. Maximal tetanic tension develops with these very high stimulation frequencies.

Experiment Data:

Voltage Length Stimuli/sec Active Force Passive Force Total Force

8.5 75 50 5.12 0.0 5.12
8.5 75 130 5.88 0.0 5.88
8.5 75 140 5.91 0.0 5.91
8.5 75 142 5.92 0.0 5.92
8.5 75 144 5.94 0.0 5.94
8.5 75 146 5.95 0.0 5.95
8.5 75 148 5.95 0.0 5.95
8.5 75 150 5.95 0.0 5.95

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Post-lab Quiz Results
You scored 0% by answering 0 out of 3 questions correctly.

1. The term tetanus refers to

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Correct answer: b. sustained muscle tension due to very frequent stimuli.

2. Which of the following distinguishes a state of unfused tetanus from a state of complete (fused) tetanus?
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Correct answer: d. Muscle tension increases and decreases between different values for an unfused tetanus.

3. When the stimulus frequency reaches a value beyond which no further increases in force are generated by the muscle,
the muscle has reached its
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Correct answer: a. maximal tetanic tension.

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Review Sheet Results
1. Describe how increasing the stimulus frequency affected the force developed by the isolated whole skeletal muscle in
this activity. How well did the results compare with your prediction?
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2. Indicate what type of force was developed by the isolated skeletal muscle in this activity at the following stimulus
frequencies: at 50 stimuli/sec, at 140 stimuli/sec, and above 146 stimuli/sec.
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3. Beyond what stimulus frequency is there no further increase in the peak force? What is the muscle tension called at this
frequency?
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