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Drs. Agustinus Yuswanto, SU., PdD, Apt
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Immunomodulatory Activity of Pacar Air (Impatiens balsamina Linn.)
Dichloromethane Fraction with Haemagglutination of Titer
Antibodies and Delay Type Hypersensitivity (DTH) Methods in Mice
(Mus musculus)
ABSTRACT
Imunomodulatory activity of dichloromethane fraction was studied
by haemaglutination of titer antibodies and delay type hypersensitivity
(DTH) methods in Balb/C mice. Each method was used 5 groups of mice
which are normal control group (CMC-Na 1% p.o), positive control group
of immunostimulant (levamisole 50 mg/kg bw, p.o), positive control group
of immunosuppressant (methylprednisolone 15 mg/kg bw, i.p), and
dichloromethane fraction groups doses of 125 mg/kg bw (DF I) and 250
mg/kg bw (DF II) orally.Based on the study, both doses of
dichloromethane fraction have a potention to suppress humoral and
celullar specific immune system. It showed lower humoral antibody (HA)
titre value than normal and control group (p<0.05) at doses of 125 mg/kg
bw.Primary and secondary titer antibody value were (5,094 0,5) and
(5,937 0,5) respectively. It also showed lower percentage of right foot
thickness in DTH method at 48 hours after induction measurement
compared with normal and control groups (p <0.05) atboth doses
groups.Based on this study, dose of 125 mg /kg bwof Pacar Air (Impatens
balsamina Linn.) dichloromethane fraction was effective dose in
imunosupressant activiy on humoral and selular spesific immune system.
Keywords :Delayed Type Hypersensitivity, Haemaglutination Titer
Antibodies, Immunomodulatory, Impatiens balsamina Linn.
INTRODUCTION
Immunomodulators are substances that can modulate the immune system.
Many synthetic drugs are efficacious as immunomodulators cause side
effects, such as urticaria, agranulocytosis, toxic to the liver, and others.1
Therefore, research must be done to prove the immunomodulatory activity
of extracts and isolated plants are expected to have less side
effects.Family Balsaminaceae is one group of plants that are the main
source of naftaquinon.2 Other studies have shown that the compound is
1.4 naphtoquinon was a significant inhibitor of the lipopolysaccharide
stimulation of macrophages through immunosuppressive activity with
inhibition percentage of 97.4 to 98.9% , The same study also showed that
1.4 naphtoquinon can show promising activity in the treatment of breast
cancer and lung cancer through immunomodulatory activity and anti-
proliferative activity.3Other study showed that ethanolicextract of Impatiens
balsamina with doses of 250 and 500 mg/kg bw have immunosuppressive
activity. It also had lower activity in Adjuvant Induced Arthritis (AIA) animal
model, best percent inhibition is shown by Ethanolic Extract of Impatiens
balsamina dose 500 mg/kg bw. Ethanolic Extract of Impatiens balsamina
also has the potential to be developed as an alternative medicine for the
treatment of rheumatoid arthritis.4The purpose of this study is to observe
the imunomodulator activity of dichloromethane fraction of pacar air
(Impatiens Balsamina L.) and to determine its effectivedose.