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CELL-DYN 3000/3500/3700/3200

TROUBLESHOOTING GUIDE

Abbott Diagnostics
A Division of Abbott Laboratories
Abbott Park, IL 60064
CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide
ii 67483-101
REVISION STATUS

Revision History Pages Revised and Added


67483-101 6/2002 First Release

CELL-DYN is a registered trademark of Abbott Laboratories.


This guide was developed and produced by TLO Learning Services in Irving, TX.
Copyright 2002, Abbott Laboratories

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 iii
Notes

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 iv
CELL-DYN 3000/3500/3700/3200
TROUBLESHOOTING GUIDE

CONTENTS
RBC Diluent Syringe Overpressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
HGB Lyse Empty (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
WBC Lyse Empty (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Lyse Empty (CELL-DYN 3500/3700) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Diluent/Sheath Empty (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Reagent Empty - Diluent, Sheath, HGB Lyse (CELL-DYN 3000) . . . . . . . . . . . . . . . . . . . . . . . . 9
Reagent Empty - Diluent, Sheath, Detergent (CELL-DYN 3500/3700) . . . . . . . . . . . . . . . . . . . 11
Shear Valve Position Fault . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Vacuum Accumulator Wet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Clog Error (RBC or WIC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Flow Error (RBC or WIC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
WOC / WBC Flow Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Elevated PLT Backgrounds / LRI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Controls Out of Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Controls Out of Range - WBC / WOC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Controls Out of Range - NOC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Controls Out of Range - WIC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Controls Out of Range - RBC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Controls Out of Range - HGB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Controls Out of Range - MCV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 1
Controls Out of Range - PLT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
WIC / WOC Mismatch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
H & H Mismatch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Incomplete Aspiration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Increased RRBC Flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Background Data Unacceptable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Data Not Transmitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700 . . . . . . . . . . . . . . . . . . . . 55
WOC Flow Cell Cleaning Procedure (CELL-DYN 3000/3500/3700) . . . . . . . . . . . . . . . . . . . . 59
WOC Flow Cell Cleaning Procedure (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


2 67483-101
RBC Diluent Syringe Overpressure

RBC DILUENT SYRINGE OVERPRESSURE


RBC Diluent
RBC Diluent
Syringe
Syringe
Overpressure
Overpressure

Check center
section of shear
valve

Remove and reinstall


Is notch in Shear Valve & release
correct No pressure from RBC
position? Diluent Syringe (See
Note) Run backgrounds

Yes

Check RBC
dilution pathway Run Does error
No Problem resolved
for pinched/ backgrounds recur?
crimped tubing

Yes

Pinched/ Refer to HS or
crimped tubing dispatch service
seen?

Yes

Remove crimps/
massage tubing
Go to Diagnostics,
More, Initialization
Run backgrounds

No

Does error
No Problem resolved
recur?
NOTE:
Remove RBC diluent syringe from
bracket (do not disconnect tubing)
Yes
y Pull down slightly on plunger
y Reinitialize analyzer by going
to: Remove, clean, and
- Main reinstall shear valve &
- Diagnostics release pressure from Does error
No Problem resolved
- More RBC diluent syringe recur?
- Initialization (See Note) Run
y If cannot initialize, cycle power backgrounds
and reinitialize
Yes

Clean/replace
Refer to HS or Does error
Yes RBC Diluent
dispatch service recur?
Syringe

No

Problem resolved

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 3
HGB Lyse Empty (CELL-DYN 3200)

HGB LYSE EMPTY (CELL-DYN 3200)


HGB Lyse Empty
HGB Lyse3200)
(CELL-DYN Empty
(CELL-DYN 3200)

Press CLEAR
FAULT and
observe HGB Lyse
Syringe

Is syringe
Clean or replace Does error
moving No No Problem resolved
syringe recur?
properly?

Yes
Yes
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom? Dispatch Service
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe

Disable analyzer,
Is syringe filled exercise valve 24
properly? Are using valve puller Does error
No
reagent lines not recur?
crimped? Massage and reseat
tubing in valve 28

Yes
Yes

Refer to HS or Cycle power


dispatch service

Does error
Dispatch Service Yes No Problem resolved
recur?

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


4 67483-101
WBC Lyse Empty (CELL-DYN 3200)

WBC LYSE EMPTY (CELL-DYN 3200)


WBC Lyse Empty
WBC Lyse3200)
(CELL-DYN Empty
(CELL-DYN 3200)

Press CLEAR
FAULT and
observe WBC
Lyse Syringe

Is syringe
Clean or replace Does error
moving No No Problem resolved
syringe recur?
properly?

Yes Yes

Check reagent cube and inlet lines: Dispatch Service


- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe

Disable analyzer, and


exercise valve
Is syringe filled
25 using valve puller
properly? Are Does error
No No Problem resolved
reagent lines not recur?
Massage and reseat
crimped?
tubing in valve
(normally closed) 23

Yes
Yes

Refer to HS or
dispatch service
Cycle power

Does error
No Problem resolved
recur?

Yes

Dispatch Service

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 5
Lyse Empty (CELL-DYN 3500/3700)

LYSE EMPTY (CELL-DYN 3500/3700)


L y s e E m p ty
( C E L L - D LYyNs e3 5E0m0 p/3t y7 0 0 )
(C E L L -D Y N 3 5 0 0 /3 7 0 0 )

C h e c k r e a g e n t c u b e a n d in le t lin e s :
- c u b e e m p ty ?
- r e a g e n t c o r r e c t?
- in le t lin e r e a c h e s to b o tto m ?
- v e n t h o le in c a p is o p e n ?
- fo a m s e a l r e m o v e d fr o m u n d e r c a p ?
- lin e s n o t c r im p e d ?
- p o r ts n o t p lu g g e d ?
- c h e c k flu id le v e ls in s y r in g e
P r im e ly s e t o w a s t e c h a m b e r
2 b y r e m o v in g t u b in g f r o m
P re s s C L E A R F A U L T V 3 6 (N C V )

F lo s s tu b in g in to N C V 3 6

E x e r c is e s o le n o id s D o e s e rro r
Yes
42, 47 re c u r?

No

D o e s e rro r
No P r o b le m r e s o lv e d
re c u r?

Yes

C h e c k W IC /H G B L y s e S y r in g e :
- s e a te d a n d m o v in g c o r r e c t ly ?
- tu b in g c r im p e d ?
C le a n /r e s e a t s y r in g e a s n e e d e d
a n d r e m o v e c r im p s

D o e s e rro r
No P r o b le m r e s o lv e d
re c u r?

Yes

C h e c k ly s e in le t lin e fo r c r im p s No
o r p lu g s
R e m o v e ly s e in le t tu b in g & f lu s h
lin e w ith w a rm D I w a te r

D o e s e rro r D o e s e rro r
P r o b le m r e s o lv e d No Yes C y c le P o w e r
re c u r? re c u r?

Yes

R e fe r to H S o r
d is p a tc h s e r v ic e

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


6 67483-101
Diluent/Sheath Empty (CELL-DYN 3200)

DILUENT/SHEATH EMPTY (CELL-DYN 3200)


Diluent/Sheath Empty
Diluent/Sheath
(CELL-DYN Empty
3200)
(CELL-DYN 3200)

Press CLEAR FAULT and


open front left door

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe

Run background

Does error
Problem resolved No
recur?

Yes

Press CLEAR FAULT, run


background, and observe
diluent and sheath reservoirs

Does fluid reach the Check reservoirs


No
sensor electrodes? for cracks / leaks

Yes
Check sensor
contacts at top of
Refer to HS or
reservoirs are
dispatch service Cracks / leaks
No seated properly.
seen?
Reseat if
indicated. Run
background count.
Yes

Dispatch service

Does error
No Problem resolved
recur?

Yes
Page 1 of 2
Go to
A

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 7
Diluent/Sheath Empty (CELL-DYN 3200)

DILUENT/SHEATH EMPTY (CELL-DYN 3200 )


A

Check for salt


buildup at
reservoir
connectors
and clean if
present

Does error
Problem resolved No
recur?

Yes

- Verify diluent/sheath plumbing is free


of restrictions
- Exercise valves 61, 62, 63, and 64
- Are reservoirs cracked and leaking?
- Clean sensor contacts (electrodes
and connector at top of reservoir) and
contacts are touching

Run background

Does error
Problem resolved No
recur?

Yes

Cycle power

Does error
Problem resolved No
recur?

Yes

Refer to HS or
dispatch service

Page 2 of 2

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


8 67483-101
Reagent Empty - Diluent, Sheath, HGB Lyse (CELL-DYN 3000)

REAGENT EMPTY - DILUENT, SHEATH, HGB LYSE (CELL-DYN 3000)


CELL-DYN 3000
CELL-DYN
Reagent Empty3000
Reagent
Diluent Empty
Diluent
Sheath
HGBSheath
Lyse
HGB Lyse

Clear fault

Press RUN and


observe reagent
reservoirs

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
- inlet line reaches to bottom?
Does fluid
- vent hole in cap is open?
reach sensor No
- foam seal removed from under
contacts?
cap?
- lines not crimped?
- ports not plugged?
Yes - check fluid level in syringe

Clean sensor
Run background
contacts
Ensure sensor is
seated correctly

Does error
No Problem resolved
recur?

Does error
Problem resolved No
recur?
Yes
No
Yes
Check reservoir for
Does error cracks
Cycle power
recur?

Yes
Floss NCV tubing
Refer to HS or (V6-1 (Diluent), V6-2 (Sheath),
dispatch service V6-3 (HGB Lyse))
No Cracks seen?
for affected reservoir
or
replace check valve

Yes

Refer to HS or
Dispatch Service
Does error
Problem resolved No
recur?

Yes

Page 1 of 2 Go to
A

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 9
Reagent Empty - Diluent, Sheath, HGB Lyse (CELL-DYN 3000)

REAGENT EMPTY - DILUENT, SHEATH, HGB LYSE (CELL-DYN 3000)

Exercise vacuum and pressure solenoids for


affected reservoir
Diluent pressure and vacuum - V5-1, 5-2
Sheath pressure and vacuum - V5-3, 5-4
HGB Lyse pressure and vacuum - V5-5, 5-6

Does error
Problem resolved No
recur?

Yes

Cycle power

Does error
Problem resolved No
recur?

Yes

Page 2 of 2 Refer to HS or
Dispatch Service

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


10 67483-101
Reagent Empty - Diluent, Sheath, Detergent (CELL-DYN 3500/3700)

REAGENT EMPTY - DILUENT, SHEATH, DETERGENT


(CELL-DYN 3500/3700)
CELL-DYN 3500/3700
CELL-DYN 3500/3700
Reagent Empty
Reagent
Diluent Empty
Diluent
Sheath
Sheath
Detergent
Detergent

Clear fault

Press RUN and


observe reagent
reservoirs

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
- inlet line reaches to bottom?
Does fluid reach
No - vent hole in cap is open?
sensor contacts?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
Yes - check fluid level in syringe

Clean sensor
contacts
Ensure sensor is
seated correctly

Does error
No Problem resolved
recur?

Does error
Problem resolved No
recur?
Yes
No
Yes
Check reservoir for
Does error
Cycle power cracks
recur?

Yes

Floss NCV tubing for


Refer to HS or affected reservoir
dispatch service No Cracks seen?
or
replace check valve

Yes

Refer to HS or
Does error Dispatch Service
Problem resolved No
recur?

Yes

Go to
Page 1 of 2 A

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 11
Reagent Empty - Diluent, Sheath, Detergent (CELL-DYN 3500/3700)

REAGENT EMPTY - DILUENT, SHEATH, DETERGENT


(CELL-DYN 3500/3700)
A

Exercise vacuum and pressure solenoids for


affected reservoir
V5-1 Diluent pressure
V5-2 Diluent vacuum
V5-3 Sheath pressure
V5-4 Sheath vacuum
V5-5 Detergent pressure
V5-6 Detergent vacuum

Does error
Problem resolved No
recur?

Yes

Cycle power

Does error
Problem resolved No
recur?

Yes

Refer to HS or
Page 2 of 2 Dispatch Service

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


12 67483-101
Shear Valve Position Fault

SHEAR VALVE POSITION FAULT

S h e a r V a lv e
P o sSitio
h ena rF V
aua lt
lv e
P o s itio n F a u lt

D id e rro r o c c u r Is s h e a r v a lv e
C le a n a n d in s p e c t R e fe r to H S o r
a fte r c le a n in g s h e a r No d a m a g e d /p in s Yes
s h e a r v a lv e D is p a tc h s e rv ic e
v a lv e ? b ro k e n ?

Yes No

R e m o v e a n d re in s ta ll
R e in s ta ll s h e a r
s h e a r v a lv e c o rre c tly
v a lv e c o rre c tly
(C E L L -D Y N 3 0 0 0 :
u s e lu b ric a n t)

R e in itia liz e s y s te m

D o e s e rro r
No P ro b le m re s o lv e d
re c u r?

Yes

C y c le p o w e r

D o e s e rro r
No P ro b le m re s o lv e d
re c u r?

Yes

R e fe r to H S o r
d is p a tc h s e rv ic e

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 13
Vacuum Accumulator Wet

VACUUM ACCUMULATOR WET

Vacuum
Vacuum
Accumulator W et
Accumulator W et

Observe waste
line for
obstructions or
correct draining

Is waste line Remove


draining No obstruction/reseat
correctly? tubing

Yes

Drain accumulators

CELL-DYN 3000: Follow Operator's Manual for


manual procedure
CELL-DYN 3500/3700/3200: through
DIAGNOSTICS menu
(go to Diagnostics, More, Drain accumulator)

Reinitialize system

Does error
No Problem resolved
recur?

Yes

Cycle power

Does error
No Problem resolved
recur?

Yes

Refer to HS or
dispatch service

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


14 67483-101
Clog Error (RBC or WIC)

CLOG ERROR (RBC OR WIC)

C log E rror (R B C or W IC )
C log E rror (R B C or W IC )

R un background

D oes error
P roblem resolved No
recur?

Y es P roblem resolved

C heck reagent cube and inlet lines: No


- cube em pty?
- reagent correct?
- inlet line reaches to bottom ?
D oes error
- vent hole in cap is open?
recur?
- foam seal rem oved from under cap?
- lines not crim ped?
- ports not plugged?
- check fluid level in syringe
Y es

R B C C log:
- clean/replace 10 m L syringe
- clean air filter
D oes error - run autoclean
P roblem resolved No
recur?
V erify aperture plate(s) are correctly
installed and check for cracks
R eseat and clean per O P S m anual
Y es

E xercise solenoids around affected


transducers and m assage and reseat
tubing at solenoids
(S ee N ote)

NOTE:
S olenoids D oes error
C E LL-D Y N 3000: No P roblem resolved
recur?
3-1, 3-2, 3-3, 3-6, 3-7, 3-8

C E LL-D Y N 3500/3700:
R B C : 3-1, 3-2, 3-3, 3-4, 3-5, 3-7, 3-8 Y es
W IC : 8-1, 8-2, 8-3, 8-4, 8-5, 8-7, 8-8

G o to
A

P age 1 of 2

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 15
Clog Error (RBC or WIC)

CLOG ERROR (RBC OR WIC)

Check
transducer(s) and
metering tubes for
cracks or leaks

Check quality of
Cracks/leaks
meniscus in No
seen?
metering tube

Yes
Check fitting between top
of metering tube and V 3-
7 (RBC) or V 8-7 (W IC) Good
No Dispatch Service
meniscus?
Clean or flush metering
tube if needed

Yes

Does error
Problem resolved No Yes Cycle power
recur?

Does error Refer to HS or


Page 2 of 2 Problem resolved No Yes
recur? dispatch service

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


16 67483-101
Flow Error (RBC or WIC)

FLOW ERROR (RBC OR WIC)


Flow Error
(RBCFlow Error
or WIC)
(RBC or WIC)

Run background

Does error
Problem resolved No
recur?

Yes

Inspect transducer/vent solenoids and


pinch tubing

Massage and reseat tubing in


solenoids as needed
SEE NOTE

Does error
Problem resolved No
recur?

Yes

Verify aperture plate(s) are


correctly installed and not
cracked
Reseat/replace if indicated

Does error
Problem resolved No
recur?

Yes

Check
transducer(s) and
metering tubes for
cracks or leaks
NOTE:
Solenoids
CELL-DYN 3000:
3-1, 3-2, 3-3, 3-6, 3-7, 3-8
Cracks/leaks Go to
CELL-DYN 3500/3700: No
seen? A
RBC: 3-1, 3-2, 3-3, 3-4, 3-5, 3-7, 3-8
WIC: 8-1, 8-2, 8-3, 8-4, 8-5, 8-7, 8-8

Yes

Dispatch Service
Page 1 of 2

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 17
Flow Error (RBC or WIC)

FLOW ERROR (RBC OR WIC)


A

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe

Run background

Does error
Problem resolved No
recur?

Yes Note:
To clean RBC or WIC metering tube:
Check quality of CELL-DYN 3000: V38 (RBC)
meniscus in CELL-DYN 3500/3200: V38 (RBC), V88 (WIC)
metering tube -- it
should look like an 1. Disconnect tubing above V38 or V88.
upside down U. 2. Connect 20 mL syringe filled with 20% bleach
to tubing.
3. Open V38 or V88 until syringe is half empty.
Check fitting between top of 4. Release solenoid and wait 5 minutes.
metering tube and V 3-7 5. Open V38 or V88 until syringe is empty and
(RBC) or V 8-7 (WIC)and Good release solenoid.
No 6. Fill syringe with HGB Lyse reagent and repeat
exercise valves if indicated meniscus?
Clean metering tubes step 5.
(See Note) 7. Run background.

Yes

Does error
Problem resolved No Yes Run autoclean
recur?

Does error
No Problem resolved
recur?

Yes

Cycle power

Refer to HS or Does error


Yes No Problem resolved
Dispatch Service recur?

Page 2 of 2

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


18 67483-101
WOC / WBC Flow Errors

WOC / WBC FLOW ERRORS

WOC/WBC Flow
WOC/WBC
Errors Flow
Errors

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
Is error occurring - inlet line reaches to bottom?
on one sample No - vent hole in cap is open?
only? - foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Yes

Rerun sample
Does error
Yes recur? No

Does error
Problem resolved No Drain/Fill reagents Replace reagents
recur?

Yes
Does error
No Problem resolved
Verify Results by an recur?
alternate method

Yes

Verify syringes:
- seated correctly
- moving correctly
- bubble free

See NOTE

Clean or replace
Syringe OK? No
as needed

Yes
Does error
NOTE: No Problem resolved
recur?
Check the following syringes:
CELL-DYN 3000: WBC metering syringe
CELL-DYN 3200: Sample injection syringe
CELL-DYN 3500/3700: WOC metering sheath syringes Go to
Yes
A

Page 1 of 2

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 19
WOC / WBC Flow Errors

WOC / WBC FLOW ERRORS


A

Check transfer
peristaltic pump tubing
for wear or holes
Replace as needed

SEE NOTE

Does error
No Problem resolved
recur?

Yes

Verify WOC/WBC mixing chamber


fills correctly, bubble mix is good
(Swirl mix for CELL-DYN 3200)

Check for pinched


Mixing tubing
No
chamber OK? Exercise bubble
mix solenoid 2-4

Yes

Check channel
offsets
Run background
(<2.0, N/A on
CELL-DYN 3200)

Offsets in Does error


Dispatch Service No No Problem resolved
specs? recur?

No
Yes Yes
Yes
Does error
Cycle power
Clean Shear Valve recur?

Yes

Does error Does error


Cycle power Yes No Problem resolved Dispatch Service
recur? recur?

No
NOTE:
Problem resolved CELL-DYN 3000: WBC transfer peripump tubing
CELL-DYN 3200: Sample transfer peripump tubing
Page 2 of 2 CELL-DYN 3500/3700: WOC transfer peripump tubing

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


20 67483-101
Elevated PLT Backgrounds / LRI

ELEVATED PLT BACKGROUNDS / LRI


Elevated PLT
Elevated/ PLT
backgrounds LRI
backgrounds / LRI

Check that ground wire is


attached, front covers on Repeat
background

Does problem
Problem resolved No
recur?

Yes

Run electrical Is it a CELL-


No Yes Empty/fill flow cell
background DYN 3200?

Is electrical
Go to Does problem
No background = No Problem resolved
C recur?
0?

Yes Yes

Check diluent/sheath Check diluent/sheath


reagent: reagent:
- correct list #? - correct list #?
- frozen? - frozen?
Replace if needed and prime Replace if needed and prime
twice after replacing. twice after replacing

Does problem
Problem resolved No
recur?

Yes

Run autoclean

Does problem Go to
Problem resolved No Yes
recur? A

Page 1 of 4

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 21
Elevated PLT Backgrounds / LRI

ELEVATED PLT BACKGROUNDS / LRI


A

C h e c k D ilu e n t (D ilu e n t/S h e a th


s y rin g e fo r C E L L -D Y N 3 2 0 0 )
s y rin g e fo r le a k s / b u b b le s /b u ild u p
C le a n o r re p la c e if in d ic a te d

R u n b a c k g ro u n d

D o e s p ro b le m
P ro b le m re s o lv e d No
re c u r?

Yes

C le a n R B C /P L T A p e rtu re P la te
and
(C E L L -D Y N 3 0 0 0 /3 5 0 0 /3 7 0 0 o n ly )
s h e a r v a lv e a s p re c a u tio n

R u n b a c k g ro u n d

D o e s p ro b le m
P ro b le m re s o lv e d No
re c u r?

Yes

C E L L -D Y N 3 0 0 0 /3 2 0 0 : C h e c k /
re p la c e (if in d ic a te d ) d ilu e n t/
s h e a th filte r

R u n b a c k g ro u n d

D o e s p ro b le m
P ro b le m re s o lv e d No
re c u r?

Yes

D ra in /fill b a th s a n d re s e rv o irs
(flo w c e ll o n C E L L -D Y N 3 2 0 0 )

D o e s p ro b le m G o to
P ro b le m re s o lv e d No Yes
re c u r? B

P age 2 of 4

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


22 67483-101
Elevated PLT Backgrounds / LRI

ELEVATED PLT BACKGROUNDS / LRI


B

Perform prepare
for shipping
procedure

Run 3-5
backgrounds

Does problem
Problem resolved No
recur?

Yes

Clean or drain
vacuum
accumulators

Run background

Does problem
Problem resolved No
recur?

Yes

Clean fan filters

Run background NOTE:


Transducer cleaning

1. From Main menu, press:


Does problem Special Protocols, Empty Xducers
Problem resolved No Yes Clean waste line 2. Disconnect vent tubing at top of transducer (at top
recur?
right port of first transducer)
3. Connect 20 mL syringe filled with 20% bleach
solution to top of the port
4. Inject bleach solution until the left side of
transducer is half filled and reconnect tubing.
5. Allow bleach to soak for 5 minutes.
6. Press: Fill xducers Main menu Run
Does problem
Problem resolved No Run background 7. Run 5 backgrounds
recur?

Yes

Check transducer electrodes for


corrosion Does problem
Yes Cycle power
Clean transducer if indicated recur?
(to clean transducer, see note:)

No

Does problem Refer to HS or


Problem resolved No Yes
recur? Dispatch Service

Page 3 of 4

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 23
Elevated PLT Backgrounds / LRI

ELEVATED PLT BACKGROUNDS / LRI


C

Check for power cords resting on


diluent or waste lines
Remove if found

Run background

Does problem
Problem resolved No
recur?

Yes

Check waste sensor dummy


plug (if used)
Reseat plug

Does problem
Problem resolved No
recur?

Yes

Clean fan filters, drain or clean vacuum


accumulators, and waste line

Run background

Does problem
Problem resolved No
recur?

Yes

Check environment:
- printer/other devices on or near CELL-DYN? (e.g.: centrifuge, air conditioning)
- flickering/fluorescent lights?
- humidity?
Problem resolved
- CELL-DYN connected to UPS?

Remove potential interferences


No

Does problem Does problem


Problem resolved No Yes Cycle power
recur? recur?

Yes

Refer to HS or
Dispatch Service
Page 4 of 4 Yes

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


24 67483-101
Controls Out of Range

CONTROLS OUT OF RANGE


Controls Out of
Controls
Range Out of
Range

- Verify QC means/limits
- Check storage/handling
- Condition of QC when
received
- Rerun controls

Does problem
No Problem resolved
recur?
Check reagent cube and inlet lines:
- cube em pty?
Yes - reagent correct?
- inlet line reaches to bottom ?
- vent hole in cap is open?
- foam seal removed from under cap?
Are backgrounds Troubleshoot
No - lines not crimped?
within specs? backgrounds No - ports not plugged?
- check fluid level in syringe

Yes
Does problem
occur in one mode
Run Fresh
only?
Controls
Does problem
No Problem resolved
Yes Yes recur?

Troubleshoot aspiration
Does problem system of affected system Yes
recur?

Check sample aspiration


through Shear Valve
No Does problem
recur?
Problem resolved No

Yes
Are sam ples Go to Incomplete
No
aspirated correctly? Aspiration Tree
Run precision

Yes

Troubleshooting Precision within Yes


No Run autoclean
im precision specs?

Yes

Verify mode to mode Does problem


No Problem resolved
calibration recur?
Recalibrate if needed

Yes

Does problem
Problem resolved No
recur? Go to OPEN
/ CLOSED
m ode

Page 1 of 10

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 25
Controls Out of Range

CONTROLS OUT OF RANGE


Problem occuring
in OPEN or
CLOSED mode

Issue resolved
OPEN /
CLOSED
No

Were Controls Controls still Try a different Controls still


No Yes
rerun? out of range? box of controls out of range?

No Yes
Yes
Issue resolved
Were controls mixed by
hand, warmed to room
Check affected temperature for 15
Yes
parameters minutes, and stored
No inside refrigerator shelf?

No
Are multiple
parameters out of No Redo controls
range? mixed by hand

Yes Follow flow chart


for affected
Clean shear valve, clean Y-fitting parameter Controls within
(for CELL-DYN 3000/3500/3700) range?
or Y-valve (for CELL-DYN 3200)
and rerun Controls

WOC/
NOC WIC RBC HGB MCV PLT
WBC

Yes

Go to Go to Go to Go to Go to Go to Go to
A B C D E F G
Does problem
Problem resolved No
recur?
Yes

Issue resolved
Yes
Check syringes:
- seated/moving
properly?
- filling?
Clean/replace as
needed

Does problem
Problem resolved No
recur?

Page 2 of 10

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


26 67483-101
Controls Out of Range - WBC / WOC

CONTROLS OUT OF RANGE - WBC / WOC


A

Run precision

Verify Sample Injection Syringe


and/or WOC Metering Syringe
are not leaking, have no
bubbles, and are moving up/
Precision within
Rerun precision down properly No
specs?
(visually inspect)

- change Transfer Pump


Tubing Yes

Empty/fill WOC Issue resolved


Is precision
No flow cell and rerun
within range?
controls
Yes

Yes Clean WOC flow


Does problem cell or perform Controls in
Problem resolved No Yes
recur? clean for ship and range?
Issue resolved rerun controls

Run normal
No
patient sample

Is placement of
Replace WOC
neutrophil population
No peripump tubing
correct on 0/10
and rerun controls
scattergram?

Yes

Check channel offsets


(CELL-DYN 3000/3500/3700 only)

Are channel
Dispatch service No
offsets < 2.00?

Yes

Verify correct operation of


syringes
(see NOTE)

Clean or replace as needed

NOTE:
Check the following syringes:
CELL-DYN 3000: WBC metering syringe & WOC sheath syringe
CELL-DYN 3200: Sample injection syringe
CELL-DYN 3500/3700: WOC metering syringe & WOC sheath Does problem
No Problem resolved
syringe recur?

Yes
Page 3 of 10
Go to
AA

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 27
Controls Out of Range - WBC / WOC

CONTROLS OUT OF RANGE - WBC / WOC

AA

Check WBC/WOC mixing


chamber for proper
draining, filling & bubble
mix
Exercise mix chamber
solenoids

Is mixing chamber
Dispatch service No draining/filling
correctly?

Yes

Verify WBC/WOC calibration by performing


a calibration verification and calibrate
if indicated

Run controls

Is the WOC
Problem resolved No
within ranger?

Yes

Dispatch service

Page 4 of 10

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


28 67483-101
Controls Out of Range - NOC (CELL-DYN 3200 Only)

CONTROLS OUT OF RANGE - NOC (CELL-DYN 3200 ONLY)


B

Check reagent cube and inlet lines:


- cube empty?
Run precision - reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
Verify sample injection syringe - foam seal removed from under cap?
or WOC metering syringe is not - lines not crimped?
leaking, has no bubbles, and is - ports not plugged?
moving up/down properly Is precision - check fluid level in syringe
Rerun precision No
(visually inspect) within specs?

- change Transfer Pump


Tubing
Yes

Are HGB Check solenoid 41


Is precision
No results No Massage and
within range?
affected? reseat tubing

Yes Yes

Clean shear valve Does problem


Issue resolved No Problem resolved
and HGB flow cell recur?

Yes
Does problem
Problem resolved No Check sample
recur?
injection syringe

Clean/replace as
Yes needed
Check HGB Lyse Syringe:
- seated/moving properly?
- filling?
Clean or replace as Does problem
needed No Problem resolved
recur?

Yes
Does problem
Problem resolved No
recur?
Verify NOC calibration by
performing a calibration
verification and
Yes calibrate if indicated
Rerun controls
Check staging
pathway from
shear valve to
HGB flow cell for
obstructions Does problem
No Problem resolved
recur?

Yes
Does problem
Problem resolved No
recur?
Refer to HS or
dispatch service

Yes

Refer to HS or
Page 5 of 10 dispatch service

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 29
Controls Out of Range - WIC

CONTROLS OUT OF RANGE - WIC


C

Refer to HS or Check reagent cube and inlet lines:


dispatch service - cube empty?
Run Precision
- reagent correct?
No - inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
Troubleshoot imprecision - lines not crimped?
Is precision by cleaning WIC aperture Is precision - ports not plugged?
No - check fluid level in syringe
within range? plate within specs?
Redo precision

Yes

Rerun
Yes
controls

Is WIC within
Problem resolved Yes
range?

No

Verify transducer operation by Solenoids


emptying, filling, draining & bubble mix 8-1, 8-2, 8-3, 8-4,
Exercise transducer solenoids, See chart of 8-5, and 8-7
massage and reseat tubing solenoids
to exercise

Is transducer
Refer to HS or
No operating
dispatch service
correctly?

Yes

Check WIC/HGB Lyse and


Diluent syringes
Clean/replace as needed

Does problem
Problem resolved No
recur?

Yes

Verify WIC calibration by


performing a calibration
verification
Calibrate if indicated
Rerun controls

Does problem Refer to HS or


Problem resolved No Yes
recur? dispatch service

Page 6 of 10

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


30 67483-101
Controls Out of Range - RBC

CONTROLS OUT OF RANGE - RBC


D

Run precision

Clean shear valve (ensure


inserted correctly)
Replace sample peripump
Troubleshoot Is precision tubing
No (except CELL-DYN 3200)
Imprecision within specs?

Yes

Does problem
Clean RBC aperture plate No Problem resolved
recur?
(except CELL-DYN 3200)

Yes

Massage tubing through solenoid


Does problem
Problem resolved No 5-4 and verify RBC chamber is
recur?
draining, filling, and swirl mixing
properly
for CELL-DYN 3200
Yes

Verify transducer operation


Solenoids
Exercise transducer solenoids,
3-1, 3-2, 3-3, 3-4, Does problem
massage and reseat tubing No Problem resolved
3-5, and 3-7 recur?
(except CELL-DYN 3200)

Yes
Is transducer
Refer to HS or
No operating
dispatch service CELL-DYN 3200:
correctly?
check vacuum #2
(3.5" open,
5.0" closed)
Yes

CELL-DYN 3200
Verify RBC mixing chamber
for proper fill/drain; swirl mix
Vac #2 within Refer to HS or
No
specs? dispatch service

Check Diluent syringe


Clean/replace as needed Yes

Verify RBC calibration by


performing calibration
verification using calibrator
Does problem Calibrate if indicated
Problem resolved No
recur?

Refer to HS or Does problem


Yes No Problem resolved
dispatch service recur?

Page 7 of 10

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 31
Controls Out of Range - HGB

CONTROLS OUT OF RANGE - HGB


E

Check reagent cube and inlet lines:


- cube empty?
Issue resolved - reagent correct?
Run precision
- inlet line reaches to bottom?
Yes - vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
Troubleshoot HGB - ports not plugged?
imprecision by - check fluid level in syringe
Is precision Is precision
cleaning HGB No
within range? within specs?
Flow Cell and
rerun precision

Yes

Check HGB reference and HGB


voltage within specs and document
No

HGB reference
within specs?

No

HGB Reference
Clean HGB flow >2200 for CELL-DYN 3000/ Refer to HS or
No Yes
cell 3500/3700 dispatch service
>2050 for CELL-DYN 3200

Refer to HS or HGB reference Check HGB syringes


No
dispatch service within specs? Check:
CELL-DYN 3200 Solenoids 2-4
-- 2-8 and massage tubing
CELL-DYN 3500/3700
Solenoid 3-6
Clean/replace as needed
Yes Yes
Rerun controls

Does problem Does problem


Problem resolved No No Problem resolved
recur? recur?

Yes

Verify HGB calibration by performing the


calibration verification
Calibrate if indicated

Refer to HS or Does problem


Yes No Problem resolved
dispatch service recur?

Page 8 of 10

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


32 67483-101
Controls Out of Range - MCV

CONTROLS OUT OF RANGE - MCV


F

Check reagent cube and inlet lines:


- cube empty?
Run precision - reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
Troubleshoot Is precision - check fluid level in syringe
No
Imprecision within specs?

Yes

Clean RBC Aperture Plate and RBC / PLT


Transducer
(CELL-DYN 3000/3500/3700 only)

CELL-DYN 3200: verify RBC mixing chamber


is draining and swirl mixing properly
Replace Diluent / Sheath if indicated

Does problem
Problem resolved No
recur?

Yes

Check RER data


(CELL-DYN 3000/3500/3700 only)
25-35% for normal patient

Refer to HS or Is RER data


No
dispatch service within specs?

Yes

Verify MCV calibration by performing


calibration verification
Calibrate if indicated
Rerun controls

Does problem Refer to HS or


Problem resolved No Yes
recur? dispatch service

Page 9 of 10

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 33
Controls Out of Range - PLT

CONTROLS OUT OF RANGE - PLT


G

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
Run precision
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Troubleshoot imprecision by
cleaning RBC aperture plate
Is precision
or replacing Diluent / Sheath No CELL-DYN 3200:
within specs?
filter (for CELL-DYN 3200) RBC / PLT
Rerun precision mix chamber; swirl

Yes

Rerun controls CELL-DYN 3200:


massage tubing
through solenoid 5-4

Does problem Does problem


Problem resolved No No Problem resolved
recur? recur?

Yes Yes

Verify transducer operation Verify PLT calibration


Exercise transducer solenoids, by performing
Is precision
No massage and reseat tubing calibration verification
within range?
(CELL-DYN 3000/3500/3700
only) Calibrate if indicated
Rerun controls

Yes
Yes Is transducer
Refer to HS or Does problem
No operating No Problem resolved
dispatch service recur?
correctly?

Yes Yes
Issue resolved

Check Diluent Refer to HS or


syringe dispatch service
Clean/replace as
needed

Does problem
Problem resolved No
recur?

Page 10 of 10

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


34 67483-101
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)

WIC / WOC MISMATCH (CELL-DYN 3500/3700 ONLY)


W IC / W OC
Mism atch
(m ultiple sam ples)

Run controls in
open m ode

Are W IC and
No Is W IC correct? No Troubleshoot W IC
W OC correct?

Yes Yes
Go to
D
Troubleshoot Troubleshoot
closed m ode W OC

Go to
A
Run precision

Troubleshoot Precision within


No
im precision specs?

Yes

Check closed
m ode aspiration

See Incom plete


Aspiration tree

Yes

Does problem
Problem resolved No
recur?

Yes

Verify m ode to
m ode calibration
Calibrate if
indicated

Does problem
Problem resolved No
recur?

Page 1 of 6

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 35
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)

WIC / WOC MISMATCH

Run precision

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
- inlet line reaches to bottom?
Troubleshoot Precision within
No Yes - vent hole in cap is open?
imprecision specs?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe

Does problem
Problem resolved No
recur?

Yes

Check W OC Sheath and metering syringes:


- filled/seated/ moving properly?

Clean/replace if needed

Does problem
Problem resolved No
recur?

Yes

Check sample
aspirations/shear
valve

Is sample
Go to Incomplete
No aspirating
Aspiration tree
correctly?

Yes

Go to
Page 2 of 6 B

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


36 67483-101
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)

WIC / WOC MISMATCH


B

Check WOC
mixing chamber

Check solenoids
Does chamber
1-7, 2-3, 2-4
No drain/fill and bubble
Massage and
mix properly?
reseat tubing

Yes

Replace WOC
Does problem
Problem resolved No Yes peristaltic pump
recur?
tubing

Does problem
Problem resolved No
recur?

Yes

Check staging pathway from WOC


mixing chamber to flow cell:
Check solenoids 2-1, 2-2, 2-3

Massage and reseat tubing

Does problem
Problem resolved No
recur?

Yes

Massage and reseat tubing


in solenoids 2-5, 2-7

Does problem Go to
Problem resolved No
recur? C

Page 3 of 6

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 37
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)

WIC / WOC MISMATCH

Check channel
offsets

Are channel
Dispatch service
offsets less No
or refer to HS
than 2.00?

Yes

Verify WOC
calibration
Recalibrate if
indicated

Does problem
Problem resolved No
recur?

Yes

Dispatch Service
Page 4 of 6

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


38 67483-101
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)

WIC / WOC MISMATCH


D

Run Precision

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
- inlet line reaches to bottom?
Troubleshoot Precision within
No Yes - vent hole in cap is open?
imprecision specs?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe

Does problem
Problem resolved No
recur?

Yes

Check WIC/HGB diluent and


lyse syringes:
- filled/seated/moving properly?

Clean/replace if needed

Does problem
Problem resolved No
recur?

Yes

Clean WIC
Aperture Plate

Does problem
Problem resolved No
recur?

Yes

Go to
E

Page 5 of 6

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 39
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)

WIC / WOC MISMATCH


E

Check W IC
transducer

Is left cham ber of


Check solenoids 8-3, 8-4 W IC transducer
No
Massage and reseat tubing draining/filling
properly?

Check tubing between top


of transducer and vent
Does problem
Problem resolved No Yes accum ulator for crim ps
recur?
Massage and reseat
tubing

Does problem
Problem resolved No
recur?

Yes

Check sam ple


aspiration/shear
valve

Is sam ple
Go to Incom plete
No aspirating
Aspiration tree
correctly?

Yes

Verify W IC
calibration
Recalibrate if
indicated

Does problem
Problem resolved No
recur?

Yes

Dispatch Service
Page 6 of 6

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


40 67483-101
H & H Mismatch

H & H MISMATCH

H & H Mismatch
H & H Mismatch

Run controls to
determine affected
parameters

Run precision

Is precision Troubleshoot
No
within specs? imprecision

Yes

Affected
parameter

MCV RBC HGB

Go to Go to Go to
A B C

Page 1 of 5

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 41
H & H Mismatch

H & H MISMATCH
C heck reagent cube and inlet lines:
- cube em pty?
- reagent correct?
- inlet line reaches to bottom ?
A - vent hole in cap is open?
- foam seal rem oved from under cap?
- lines not crim ped?
- ports not plugged?
- check fluid level in syringe

D oes problem
P roblem resolved No
recur?

Y es

R un autoclean

D oes problem
P roblem resolved No
recur?

Y es

C lean R B C /P LT aperture plate


(C E LL-D Y N 3000/3500/3700 only)

D oes problem
P roblem resolved No
recur?

Y es

C lean fan filters

D oes problem
P roblem resolved No
recur?

Y es

P erform C alibration verification


C alibrate if indicated

D oes problem
P roblem resolved No Y es D ispatch S ervice
recur?

P age 2 of 5

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


42 67483-101
H & H Mismatch

H & H MISMATCH
B

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe

Does problem
Problem resolved No
recur?

Yes

Run autoclean

Does problem
Problem resolved No
recur?

Yes

Check Diluent syringe:


- Moving/filling OK?
- Bubble free?

Clean/replace syringe

Perform
Calibration
Does problem
Problem resolved No verification
recur?
Calibrate if
indicated

Yes

Clean RBC/PLT aperture plate


(CELL-DYN 3000/3500/3700 only) Does problem
Yes No Problem resolved
recur?

Yes
Does problem
Problem resolved No
recur?
Dispatch Service

Page 3 of 5

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 43
H & H Mismatch

H & H MISMATCH
C

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe

Does problem
Problem resolved No
recur?

Yes

Run autoclean

Does problem
Problem resolved No
recur?

Yes
NOTE:
HGB References:
CELL-DYN 3000 1850-2250 Check HGB
CELL-DYN 3500 1850-2250 Reference
CELL-DYN 3700 1800-2300 See Note
CELL-DYN 3200 1800-2300

Clean HGB flow HGB Reference


No
cell within specs?

Yes

Refer to HS or HGB Reference


No Check HGB lyse
Dispatch Service within specs?
syringe:
- Moving/filling OK?
- Bubble free?
Yes
Yes Clean/replace if needed

Does problem
Problem resolved No
recur?
Does problem
No Problem resolved
recur?

Yes

Go to
Page 4 of 5 D

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


44 67483-101
H & H Mismatch

H & H MISMATCH

Exercise solenoids:
CELL-DYN 3000/3500/3700: V 1-3
CELL-DYN 3200: V2-4
Massage/reseat tubing

Does problem
Problem resolved No
recur?

Yes

CELL-DYN 3500/3700:
Exercise WIC transducer drain
solenoids V8-2 and V8-3
Massage and reseat tubing

Does problem
Problem resolved No
recur?

Yes

Perform calibration
verification
Calibrate if indicated

Does problem
Problem resolved No
recur?

Yes

Dispatch Service
Page 5 of 5

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 45
Incomplete Aspiration

INCOMPLETE ASPIRATION
Incomplete
Incomplete
Aspiration
Aspiration
(multiple samples)
(multiple samples)

Does error
Go to
occur in both No
A
modes?

Yes

CELL-DYN 3000/3500/3700 CELL-DYN 3200

Inspect/change
sample peristaltic Check vacuum #2
pump tubing

Does error Vac #2 within Refer to HS or


Issue resolved No No
recur? specs? dispatch service

Yes Yes

Check sample
Flush Y-valve
sensor

Refer to HS or LED on/off Does error


No No Problem resolved
Dispatch Service appropriately? recur?

Yes

Inspect/clean
shear valve and
Yes
flush Y-fitting with
10% bleach

Does error Refer to HS or


Problem resolved No Yes
recur? Dispatch Service

Page 1 of 2

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


46 67483-101
Incomplete Aspiration

INCOMPLETE ASPIRATION
A

Closed Mode Open Mode


only only

Perform Sam ple path cleaning and include: Perform Sam ple path cleaning and include:
CELL-DYN 3000/3500/3700 : clean Y-fitting CELL-DYN 3000/3500/3700 : clean Y-fitting
CELL-DYN 3200: clean Y-valve CELL-DYN 3200: clean Y-valve

Does error Does error


Problem resolved No No Problem resolved
recur? recur?

Yes
Yes
Check and exercise solenoids:
CELL-DYN 3000/3500/3700: 9-5 (open
Check/clean m ode)
sam ple/vent CELL-DYN 3200: Exercise V1-7 and verify
needles no restrictions between Y-valve and V1-7
Massage/reseat tubing

Does error
No Problem resolved
Does error recur?
Problem resolved No
recur?

Yes
Yes
Replace aspiration
peripum p tubing
Check SL LED (for CELL-DYN
3000/3500/3700
only)

Refer to HS or Sensor LED on/off


Run a Sam ple
No appropriately?
Dispatch Service
(except CELL-DYN 3200)

Yes Does error


No Issue resolved
occur?
Exercise
CELL-DYN 3000/3500/3700: solenoid 9-6
CELL-DYN 3200: exercise V1-2 and verify no
restrictions between Y-valve and V1-2 m assage/ Yes
reseat tubing Refer to HS or
Run another
dispatch service
Sam ple
Yes
Does error Refer to HS or
Problem resolved No Yes
recur? Dispatch Service Does LED sensor to
Does error
right of shear valve Yes
recur?
turns on/off?

No
No
Refer to HS or
Issue resolved
dispatch service
Page 2 of 2

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 47
Increased RRBC Flags

INCREASED RRBC FLAGS


Increased RRBC
Increased
Flags RRBC
Flags

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe

Does problem
Problem resolved No
recur?

Yes

Clean Shear Valve

Does problem
Problem resolved No
recur?

Yes

Replace W OC
peristaltic pump
tubing

Does problem
Problem resolved No
recur?

Yes

Check W OC sheath and metering syringes


(on CELL-DYN 3200, it is the sample injection
syringe):
- seated and moving properly?
- bubble free?

Clean/replace if needed

Does problem
Problem resolved No
recur?

Yes

Go to
Page 1 of 3 A

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


48 67483-101
Increased RRBC Flags

INCREASED RRBC FLAGS


A

Check WOC mixing chamber


(on CELL-DYN 3500 and 3700 only) or clean it
if indicated - see WOC Chamber Cleaning Procedure page
60
(on CELL-DYN 3200: check WBC mixing chamber)

For CELL-DYN 3000/3500/3700: Check solenoids Does chamber drain/fill and


1-7, 2-3, 2-4 and massage and reseat tubing bubble mix properly?
No
For CELL-DYN 3200: Exercise solenoids 53, 55, 92 (on CELL-DYN 3200: does it
and massage and reseat tubing swirl mix and drain properly?)

Yes
Check staging pathway from
WOC mixing chamber to flow
Does problem cell:
Problem resolved No Yes
recur?
Massage and reseat tubing in
staging solenoids

Does problem
Problem resolved No
recur?

Yes

Check channel
offsets
(CELL-DYN 3000/
3500/3700 only)

Are channel
offsets less
than 2.00?

Yes

Check 0/10
scattergram

Are lymph/neut
populations
No Dispatch Service
- correctly positioned?
- well defined?

Yes

Go to
B
Page 2 of 3

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 49
Increased RRBC Flags

INCREASED RRBC FLAGS

Verify WOC
calibration
Recalibrate if
indicated

Does problem
Problem resolved No
recur?

Yes

Dispatch Service
Page 3 of 3

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


50 67483-101
Background Data Unacceptable

BACKGROUND DATA UNACCEPTABLE


Background Data
Background Data
Unacceptable
Unacceptable

Check that ground wire is


attached, front covers on.
Repeat background

Repeat
Problem resolved Yes background
OK?

No

Run electrical Is it a CELL-


No Yes Empty/fill flow cell
background DYN 3200?

Check for Is electrical


Does problem
electrical No background No Problem resolved
recur?
interference* OK?

Yes
Yes
Replace milipore
Does problem filter
Clean Aperture
recur?
Plates

No
Does problem
No Problem resolved
recur/
Yes Does problem
Problem resolved No
recur?

Yes

Dispatch Service Clean Transducers *Examples of electrical interference:


NOTE: See Elevated PLT - reagent lines near electrical wires
Backgrounds/LRI flow chart for Yes - printer on top of CELL-DYN
Transducer cleaning instructions - electrical appliances near CELL-
DYN
- flickering fluorescent lights

Does problem Go to
Problem resolved No Yes
recur? A

Page 1 of 2

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 51
Background Data Unacceptable

BACKGROUND DATA UNACCEPTABLE

Check reagent cube and inlet lines:


- cube empty?
- reagent correct?
- inlet line reaches to bottom? Repeat
Replace reagent
- vent hole in cap is open? background
and prime twice
- foam seal removed from under cap? OK?
- lines not crimped?
- ports not plugged?
- check fluid level inNo
syringe
Yes

Repeat
Clean vacuum accumulator
background Yes Problem resolved
Clean fan filters
OK?

No

Perform prepare
for ship

Repeat
Problem resolved Yes background No Cycle power
OK?

Yes Repeat
background
OK?

No

Dispatch Service
Page 2 of 2

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


52 67483-101
Data Not Transmitting

DATA NOT TRANSMITTING


Data not
Data not
transmitting
transmitting

Start

First time to
Has it worked
No be hooked-up
before?
to an LIS?

Yes

No Using current rev Send current


No
of interface spec? interface spec

Yes

Yes Does problem


No Problem resolved
recur?

Yes

Check computer set-up


(see NOTE)
Cable connected to proper port

Is set-up
No Correct set-up
correct?

Does problem
Yes No Problem resolved
recur?

Yes

NOTE:
Perform serial loop Go to
Refer to Operator's Manual for
back test A
computer setup

Page 1 of 2

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 53
Data Not Transmitting

DATA NOT TRANSMITTING

Pass? Failed?

Yes Yes

Dispatch Service
Cycle power and
reseat LIS cable

Does problem
No Problem resolved
recur?

Yes

Refer to LIS
vendor

Page 2 of 2

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


54 67483-101
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700

RETICULOCYTE TROUBLESHOOTING FOR CELL-DYN 3200, 3500


AND 3700
Reticulocyte
Reticulocyte
Troubleshooting for
Troubleshooting
CELL-DYN 3200, for
CELL-DYN
3500 and 37003200,
3500 and 3700

Verify that stain has been stored in the dark at room


temperature, that Reticulocyte Backgrounds <100 and
exact dilution and incubation instructions have been
followed.
Are calibrated pipettes used?

Problems with
Go to
Both patients or QC out Patients Only
A
of range or both?

Is it sample Go to
Rerun QC QC Only No
specific? A

Yes
Still out of
Issue resolved No Rerun
range?

Yes

Is issue
No Issue resolved
Prepare a new dilution recurring?
of QC and rerun

Yes

Prepare a new dilution


of same sample and
Still out of
Issue resolved No rerun
range?

Yes
Is issue
No Issue resolved
Try new vials of recurring?
QC, prepare a new
dilution and rerun
Yes

Redraw sample or verify by


alternate method
Still out of
Issue resolved No
range?

Yes

Verify pipette is calibrated Yes Is issue


and proper technique followed recurring?

No

Is issue Go to
Issue resolved No Yes
recurring? A Issue resolved

Page 1 of 4

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 55
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700

RETICULOCYTE TROUBLESHOOTING FOR CELL-DYN 3200, 3500


AND 3700
A

Is background on
the stain <100? No Rerun background

Yes

Check maintenance
- extended autoclean - weekly
Is background
- clean metering syringe Yes
<100?
- clean sheath syringe
- drain and fill flow cell

No

Try new stain and


Maintenance Redo maintenance rerun
No
up to date? and rerun controls

No
Yes Controls within
range? Yes
Controls within
No
range? Yes

Do WOC gains and


Issue resolved
reticulocyte gains
match? Issue resolved
No

Yes
Follow procedure below:
1. For WOC gains: go to Main menu, Diagnostics, F12,
F1, Set point entry (0, 10, 90), and print screen
2. For reticulocyte gains: go to Reticulocyte mode,
Diagnostics, F12, F1, WOC (0, 10, 90)
3. Compare WOC gains and reticulocyte gains (0, 10,
90)

Do WOC and
Rerun QC Yes reticulocyte gains
match?

No

Go to Go to
B C

Page 2 of 4

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


56 67483-101
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700

RETICULOCYTE TROUBLESHOOTING FOR CELL-DYN 3200, 3500


AND 3700
B C

Copy WOC gains as follows:


Enter WOC values (0, 10, 90) into
reticulocyte gains screen and
press Set analyzer to save entry

Rerun patient QC within


Yes Rerun QC
samples range?

No

Patient
Issue resolved Yes Run 3 -- 5 normal patient
samples OK? Controls within
samples in regular patient No
range? Yes
mode

No
Issue resolved

Verify by alternate Is neutrophil population


method at 0/10 scattergram at
Yes Rerun QC
the 2.5 by 2.5 hash
marks?

No

Check channel offsets are


Dispatch Service <2.0 Controls within
No
(only for CELL-DYN 3500 range? Yes
and 3700)

Issue resolved

Go to
D

Page 3 of 4

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 57
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700

RETICULOCYTE TROUBLESHOOTING FOR CELL-DYN 3200, 3500


AND 3700
D

Channel offsets within


Controls within range?
Issue resolved Yes Rerun controls Yes
range? (only for CELL-DYN 3500
and 3700)

No

Drain/fill flow cell, run autoclean, clean


WOC mixing chamber
(see WOC mixing chamber cleaning
No
procedure) and recheck channel
offsets
(only for CELL-DYN 3500 and 3700)

Controls within
Issue resolved Yes Rerun QC
range?

No Dispatch Service

References:
1. CELL-DYN 3500 Operator's Manual, June 1999: Chapter 14, 14-1 to 14-8
2. CELL-DYN 3700 Operator's Manual, November 2000: Chapter 14, 14-1 to 14-87
3. CELL-DYN 3200 Operator's Manual, October 2001: Chapter 14

Page 4 of 4

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


58 67483-101
WOC Flow Cell Cleaning Procedure (CELL-DYN 3000/3500/3700)

WOC FLOW CELL CLEANING PROCEDURE


(CELL-DYN 3000/3500/3700)

Materials/Tools Needed: 25% Bleach solution


10 mL syringe

Step Action
1 Fill syringe with 25% bleach solution.
2 From the Main Menu, press
SPECIAL PROTOCOLS
DISABLE/ENABLE ANALYZER

3 Insert bleach into WBC/WOC mixing chamber.


NOTE: Follow the steps below to avoid damaging the plastic port of
mixing chamber on CELL-DYN 3500/3700.
a. Follow tubing from mixing chamber to Overflow Chamber.
CELL-DYN 3000: top port of WBC mixing chamber
CELL-DYN 3500/3700: left top port of WOC mixing chamber
b. Disconnect tubing from overflow chamber.
c. Connect syringe to tubing.
d. Inject bleach solution and fill WBC/WOC mixing chamber 2/3 full.
e. Pull back on syringe slightly to remove any excess pressure and liquid
trapped in tubing.
f. Remove syringe and reconnect tubing to overflow chamber.
4 For Special Protocols Menu, press
ENABLE ANALYZER

5 Perform 2 autocleans with water to allow bleach to enter and clean flow cell.
6 Run 3 background counts.
7 Verify background counts are within specifications.

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


67483-101 59
WOC Flow Cell Cleaning Procedure (CELL-DYN 3200)

WOC FLOW CELL CLEANING PROCEDURE


(CELL-DYN 3200)

Materials/Tools Needed: 25% Bleach solution


10 mL syringe with tubing attached

Step Action
1 Fill syringe with 25% bleach solution.
2 From the Main Menu, press
SPECIAL PROTOCOLS
DISABLE/ENABLE ANALYZER

3 Insert bleach into WBC/WOC mixing chamber.


a. Remove tubing from mixing chamber (WBC or RBC/PLT).
b. Connect syringe and attach tubing to mixing chamber.
c. Inject bleach solution and fill WBC mixing chamber 2/3 full.
d. Pull back on syringe slightly to remove any excess pressure and liquid
trapped in tubing.
e. Remove syringe and reconnect tubing to overflow chamber.
4 For Special Protocols Menu, press
ENABLE ANALYZER

5 Perform 2 autocleans with water to allow bleach to enter and clean flow cell.
6 Run 3 background counts.
7 Verify background counts are within specifications.

CELL-DYN 3000/3500/3700/3200 Troubleshooting Guide


60 67483-101

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