Biology 10

Osmosis Lab

Phatthanakittichai Pemika Pair

Sienglue Napat Jaguar

Ounpiyodom Sasi Minnie

Kaveevorasart Tiprada Wawa

Pinyotamakorn Chanyanut Ning

1002

March 8, 2017
Introduction

Purpose
The main purpose of this jelly/potato activity is to understand the concept of osmosis,
how the water moves to the area that has more solute and to understand how the
appearance of the cell changes due to osmosis.

Hypothesis
How will soaking the Jelly in distilled water affect the size of the candy?
If we soak the jelly in distilled water, then the jelly will swell up.
How will soaking the Jelly in concentrated salt water affect the size of the candy?
If we soak the jelly in concentrated salt water, then the jelly will shrink.
How will soaking the Potato in distilled water affect the size?
If we soak the potato in distilled water, then the potato will swell up.
How will soaking the Potato in concentrated salt water affect the size?
If we soak the potato in concentrated salt water, then the potato will shrink.

Material list

6 Beakers (3 for jelly, 3 for potato)

Electronic Balance
Distilled water
Concentrated salt water
2 Jellies
2 Potato Pieces
Calculator groups bring their own calculator
Knife
Weighing paper
Markers
Gloves
Lab Coat
Goggles

Procedure/Methods
Collect the materials that your group needs (3 Jelly and Half a Potato)
Use the knife to cut the potato into 3 equal pieces
Use the electronic balance to find the mass of your jelly. Remember to lay down
a piece of weighing paper and set to zero.
Record your data
Record descriptive observations about the jelly prior to the experiment.
Measure the initial volume of the jelly and the potato and record your data.
Fill your 1st beaker halfway with distilled water. Put one of your jelly into the
beaker. Set the beaker aside for one hour. Label the beaker using marker.
Fill your 2st beaker with concentrated salt solution halfway. Put your 2nd jelly into
that beaker. Set beaker aside for one hour. Label the beaker using marker.
 Put your 3rd jelly into an empty beaker without putting anything in it. That should
be your control.
Put the 4th beaker halfway with distilled water. Put one of your potato pieces into
the beaker. Set the beaker aside for one hour. Label the beaker.
Put the 5th beaker halfway with concentrated salt solution halfway. Put your
second potato pieces into that beaker and set it aside for an hour. Label the
beaker.
Place your last piece of potato pieces into the last beaker, empty. That is your
control for the potato. Label the beaker.
Make sure you label all the beakers distilled water or concentrated salt
solution with some markers.
After one hour, come back and look at each of the beakers and record the data
volume and mass of both the jellies in two beaker and both the potato in two
other beakers.
Also record the descriptive data for what the jelly and the potato look like now
after being soaker for an hour.
Calculate the percent change in the size of each jelly and potato
** Percent change in volume
** Percent change in mass

Data Tables
Data Table A: The volume and mass of the 2 jelly before and after soaking in distilled
water and before and after soaking in salt water
Jelly 1 Jelly 2

Dimensions Initial: Before Final: After Initial: Before Final: After

Soaking Soaking in Soaking soaking in
Distilled Water Salt Water

Volume 2 ml 5.5 ml 3.5 ml 3 ml

Mass 5.18 g 6.36 g 5.10 g 3.92 g

Data Table B: The volume and mass of the 2 potatoes before and after soaking in
distilled water and before and after soaking in salt water

Potato 1 Potato 2
 Dimensions Initial: Before Final: After Initial: Before Final: After
Soaking Soaking in Soaking soaking in
Distilled Salt Water
Water

Volume 6 ml 7 ml 6 ml 5 ml

Mass 6.17 ml 6.96 ml 6.14 g 5.31 g

Data Table C: Descriptive observations of the jelly before and after soaking in distilled
water and before and after soaking in salt water

Jelly 1 Jelly 2

Descriptive Observations Descriptive Observations

Before Soaking in Distilled Water: Before Soaking in Salt Water:
Rounded Rounded
Sticky Sticky
Firm Firm
Red/purple Red/purple

Descriptive Observations After Descriptive Observations After

Soaking in Distilled Water: Soaking in Salt Water
Bigger in size Smaller in size
Looks more transparent Looks more transparent

Data Table D: Descriptive observations of the potato before and after soaking in distilled
water and before and after soaking in salt water.

Potato 1 Potato 2
 Descriptive Observations Descriptive Observations
Before Soaking in Distilled Water: Before Soaking in Salt Water:
Yellow in color Yellow color
Sinks in water Floats in water
Wet Wet
Rectangular in shape Rectangular in shape

Descriptive Observations After Descriptive Observations After

Soaking in Distilled Water: Soaking in Salt Water
Bigger in size Smaller in size
Hard texture Soft texture
Dense Brittle

Data Table E: Create a data table to record the percent changes for the jelly similar to the
one below.

Jelly 1: Soaked in Distilled Water Jelly 2: Soaked in

Salt Water

Percent Change in Volume 175% -14.29%

Percent Change in Mass 22.78% -30.10%

Data Table F: Create a data table to record the percent changes for the potato similar to
Table E above.

Potato 1: Soaked in Distilled Potato 2: Soaked in Salt

Water Water

Percent Change in 16.7% -17%

Volume

Percent Change in 12.8% -13.5%

Mass
Bar Graph of Results
Analyzing Results
1. What happened to the jellies after being soaked in distilled water and salt water
for an hour? Why? Be descriptive and detailed in your answer. Use your knowledge
of membrane transport.
Ans: After an hour, the jelly soaked in distilled water become less firm, was bigger in
size, and became more transparent. The jelly soaked in salt water shrank slightly in
size, became lighter and more brittle.The reason why their appearance changed is
because water always moves from low to high solute percentage.The distilled water has
less solute percentage otherwise called hypotonic, so the water moved from outside to
inside of the jelly. The salt water has more solute percentage otherwise called
hypertonic, so the water moved from inside the jelly to outside. This process is called
osmosis.

2. What happened to the potato after being soaked in distilled water and salt water for
an hour? Be descriptive and detailed in your answer. Use your knowledge of membrane
transport.
Ans: After an hour, the potato soaked in distilled water grew slightly larger, heavier and
harder from before. The potato soaked in salt water shrank slightly in size, became
lighter and more brittle. The reason why their appearance changed is because water
always moves from low to high solute percentage. The distilled water has less solute
percentage otherwise called hypotonic, so the water moved from outside to inside of the
potato. The salt water has more solute percentage otherwise called hypertonic, so the
water moved from inside the potato to outside. This process is called osmosis.
Conclusion
From observing and collecting datas of this lab, the results matched our
hypotheses. Both the potatoes and jellies swell when soaked in distilled water and
shrink when soaked in concentrated salt solution. By observing these changes, we have
concluded that water will always move from the side with low solute percentage to the
side with high solute percentage.

Worklog
Name Job Role

Pair Wrote the purpose, the hypotheses, the analyzing results and the conclusion.

Ning Created the data tables and the bar graphs.

Minnie Wrote the purpose, the analyzing results, the bar graphs and the conclusion.

Wawa Created the data tables, and the bar graphs

Jaguar Material & methods