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  • Microbiology Lab Report: Culturing of Microflora Bacteria Through Aseptic Technique

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    Muhammed ElRakabawi
    229 Ansichten4 Seiten
    This lab report summarizes an experiment culturing E. coli and microflora bacteria from a hair sample. The student followed aseptic techniques to prepare nutrient agar medium in petri dishes. E. coli was extracted from a conical flask and inserted into one dish, while a hair sample was placed in another. After two days of incubation, both dishes showed bacterial growth, with more abundant growth of E. coli than microflora from the hair. The aim was to culture microorganisms from different sources using sterile laboratory techniques.

    Originalbeschreibung:

    This is a lap report for culturing simple microflora bacteria from a personal hair sample through aseptic technique

    Originaltitel

    Microbiology lab report: culturing of microflora bacteria through aseptic technique

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    This lab report summarizes an experiment culturing E. coli and microflora bacteria from a hair sample. The student followed aseptic techniques to prepare nutrient agar medium in petri dishes. E. coli was extracted from a conical flask and inserted into one dish, while a hair sample was placed in another. After two days of incubation, both dishes showed bacterial growth, with more abundant growth of E. coli than microflora from the hair. The aim was to culture microorganisms from different sources using sterile laboratory techniques.

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    © All Rights Reserved
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    Markieren Sie unangemessene Inhalte
    229 Ansichten4 Seiten

    Microbiology Lab Report: Culturing of Microflora Bacteria Through Aseptic Technique

    Hochgeladen von

    Muhammed ElRakabawi
    This lab report summarizes an experiment culturing E. coli and microflora bacteria from a hair sample. The student followed aseptic techniques to prepare nutrient agar medium in petri dishes. E. coli was extracted from a conical flask and inserted into one dish, while a hair sample was placed in another. After two days of incubation, both dishes showed bacterial growth, with more abundant growth of E. coli than microflora from the hair. The aim was to culture microorganisms from different sources using sterile laboratory techniques.

    Copyright:

    © All Rights Reserved
    Als PDF, TXT herunterladen oder online auf Scribd lesen
    Markieren Sie unangemessene Inhalte
    von 4

    FacultyofBiotechnology

    MICROBIOLOGY
    MB102b
    [Lab]

    LABREPORT#1:

    Culturingofsimplemicroflorabacteria(through
    aseptictechnique)

    MuhammedSayedHassan
    ID:171741
    GROUP(F)

    Submittedto:L.A.Suzanneali

    INTRODUCTION

    Thislabcoveredusageofpre-preparedmedium(Agarnutrientmediumwhich
    isageneralpurposemediumsupportinggrowthofawiderangeof
    non-fastidiousorganisms.ThenculturingofmicrofloraandE.Colibacteria.All
    proceduresthroughaseptictechniques.

    AIM:Culturingofmicrofloraandescherichiacolibacteriainpetridishes.

    MATERIALS

    Chemicalagents:

    1. NutrientAgarMedium

    Biologicalagents:

    1. E.Coli
    2. Personalhairsample(probablycontainingsimplemicroflora)

    Equipments:

    1. Glasshood
    2. Petridish
    3. Hairsample(probablycontainingsimplemicroflora)
    4. Inoculationloop
    5. Bunsenburner
    6. Conicalflask
    7. Ethanol
    8. Markerpen

    PROCEDURE-Methodology:

    a) Petridishpreparation
    1. N.Agarmediumwasalreadypre-preparedinaconicalflask.
    2. N.Agarwaspouredinapetridishcarefullybesideabunsenburner.
    3. Allworkwasdoneinsideaglasshoodtopreventcontamination.

    b) Sterilizationofworkspace
    4. Thebenchwascleanedwithcottonandethanol.
    5. Bunsenburnerwaslitfor10-15minsinthediameterofworkingspace.
    6. Inoculationloopwithsterilizedwithbunsenburner.

    c) CulturingofE.Coli
    7. Escherichiacolibacteriawasextractedfromconicalflaskwiththesterilized
    inoculationloop.
    8. Petridishcontainingmediumwasopenedwiththethumbslightlyangledto
    preventmediumcontamination,thenE.coliwasinsertedwiththeinoculation
    loop.
    9. Allworkwasdoneinthesterilizedworkspaceinadiameterofthebunsenburner.

    d) Culturingofsimplemicroflora(Hairsample)
    10. HairsamplewasputinsidetheotherpetridishcontainingsameN.Agarmedium.
    11. Allworkwasdoneinthesterilizedworkspaceinadiameterofthebunsenburner.

    RESULTS

    Fig(1):PetridishcontainingculturedE.ColiFig(2):Petridishcontainingcultured

    bacteria,inthetophalfsection.bacteriaformahairsample.

    NOTE:[thepictureswastakenafter2daysofculturing.]

    DISCUSSION:

    Abovefigures[fig(1)andfig(2)]showsthatculturingwaspositiveacrossthetwodishes,
    theresultswasconductedafter48ofculturing,infig(1)E.Coliquantityobservedwas
    remarkablyhigherthanHairsamplemicroflorainfig(2)probablyduetomorequantity
    insertedthroughtheinoculationloop.

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