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a. The component of DNA polymerase III that makes the subunit or sliding
enzyme high processive. clamp
e. The family of enzymes that establishes the genetic code, Aminoacyl t-RNA
ie. pairs the amino acid with the nucleotide codon. synthetases
2. Draw arrows on the diagrams showing how alternative splicing of pre-mRNA can
give rise to two different proteins in neuronal and thyroid cells. The introns are
shown in white. (Assume that there is an downstream 3 splice site not shown)
(6 points)
Name ______________________________
BMB7010 Biochemistry Exam 5 Page 2 of 5
3. Match the following reactions with the corresponding enzyme in the list below
J
a. The first committed step in mammalian pyrimidine biosynthesis.
5. The fidelity of DNA replication beyond that obtained by precise base pairing is
ensured by two additional mechanisms (not mechanisms that repair damaged DNA).
What are they?
proof reading 3- 5
1.
mismatch repair (exonuclease 1, endonuclease, polymerase III)
2. (4 points)
Name ______________________________
BMB7010 Biochemistry Exam 5 Page 3 of 5
6. Write down all of the codons that a tRNA with the following anticodon loop
recognizes on the mRNA. Remember to write the codon sequence from 5' to 3'.
5 C-C-I-G-C-Pur-A 3
7. The structure of DNA presents several problems that must be overcome for
replication to occur. Name, do not explain, the mechanism that solves these problems.
One phrase or sentence only. Do not exceed the space provided.
c. The edges of the bases are on the inside of the helix and inaccessible.
helicases unwind the double helix.
9. Eurkaryotic RNA synthesizes employs three different RNA polymerases (RNA pol).
Identify which polymerases have the following characteristics.
a. synthesizes rRNA X X
X
b. synthesizes mRNA
X
c. synthesizes tRNA
X
d. sometimes has a TATA box
X
e. sometimes has a downstream
promoter element (DPE)
h. enhancers X
(10 points)
11. What is the function of the following proteins in the initiation of DNA replication in E.
coli? Draw a line connecting the protein and its function.
DnaA Helicase
DnaC Primase
12. Steroid hormones trigger a sequence of events that lead to increase transcription of
selected genes. Choose a word from the list below that best fits and write the letter in
the blank.
E
1) The steroid hormone binds to a _____.
J
3) The histone tails are then ____.
.
B
4) The modified histone tails then bind to a protein domain called the ____.