Beruflich Dokumente
Kultur Dokumente
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HK Regulation
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AOAC Official Methods
AOAC 985.29 Total Dietary Fibre in Foods Enzymatic-Gravimetric Method
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AOAC 985.29
Enzymatic
Enzymaticdigestion
digestion
Duplicate
Duplicatetest
test i)i) -amylase
-amylase Precipitation
Precipitationand
and
portions
portionsof
of filtration
ii)ii) protease
protease filtration(residue)
(residue)
sample
sample iii)
iii)amyloglucosidase
amyloglucosidase
One
Oneresidue
residuefor
for One
Oneresidue
residuefor
for
protein
proteinanalysis
analysis ash
ashanalysis
analysis
Total
Totaldietary
dietaryfibre
fibre==weight
weight(residue)
(residue)weight
weight(ash
(ash++protein)
protein)
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AOAC 985.29
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AOAC 985.29
Preparing for digestion
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AOAC 985.29
Preparing for digestion
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AOAC 985.29
-Amylase: gelatinize
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AOAC 985.29
Protease: remove protein
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AOAC 985.29
Amyloglucosidase: remove starch
Cool.
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AOAC 985.29
Summary
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AOAC 985.29
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AOAC 985.29
Precipitation
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AOAC 985.29
Filtration
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AOAC 985.29
One test portion for protein, using N x 6.25
as conversion factor.
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AOAC 985.29
Test for enzyme purity every half yearly
Test portion Expected
Test sample Activity tested (g) recovery (%)
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AOAC Method for functional fibre
Functional fibre Commercial name Test Method
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AOAC 2001.03
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AOAC 2001.03
Filtrate
Filtrate(by
(by i)i)Concentrate
Concentrate Analysed
Analysedby
byLC
LC
985.29)
985.29) ii)
ii)Desalt
Desalt
Total
Totaldietary
dietaryfibre
fibre==
[weight
[weightof
ofresidue
residue--
protein
proteinash
ash--blank]
blank]++
[weight
[weightof
oflow
lowMWMWsoluble
soluble
fibre
fibredetermined
determinedby byHPLC]
HPLC]
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AOAC 2001.03
Only a portion of
the resistant
maltodextrin is
precipitated in the
aqueous ethanol when
Method 985.29 is
applied to foods
containing resistant
maltodextrin.
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AOAC 2001.03
In Method 2001.03,
resistant
maltodextrin that
are soluble in
aqueous ethanol
are desalted,
concentrated and
measured by liquid
chromatography.
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Desalting
Ion exchange
column
(OH-type and
H-type)
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AOAC 2001.03
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Calculation of available carbohydrate
when method 2001.03 is used
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Recoveries for other functional fibres
when tested by AOAC 2001.03
Functional fibre Recovery (%)
-Glucan 101*
Fructooligosaccharides 98
Polydextrose 117
Galactooligosaccharides 111
Glucooligosaccharides 46
Resistant maltodextrin 88
* Recovery of insoluble dietary fibre and soluble dietary fibre is particular high.
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Points to note
Definition of 0: 1 g/100 g.
Results are method dependent.
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Available proficiency test
FAPAS
AOAC
LGC
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Thank You
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