Beruflich Dokumente
Kultur Dokumente
BOTULISM Gram +ve Decompose proteins Exotoxins Botulism exotoxin A,B,C1,C2,D-G Specimen Treatment
Rods Ferment a variety of sugars Teichoic ABE- principle cause of human illness - vomit, gastric lavage, feces, autopsy material, Trivalent (ABE) antitoxin neutralizes the
F: Clostridiaceae Spore-forming C. Botulism is Lipase +ve acid AB mainly in food, E in fish leftover food exotoxin
G: Clostridium - to detect toxin and pathogen
Motile , except Many clostridia can cause CD in animals
S: Botulinum H antigen Immunoprophylaxis
C.perfinges hemolysis zone G non disease causing Examination
Endospores Obligate anaerobes - peptidoglycan Trivalent toxoid (ABE)
-enteral clostridiosis Toxins controlled by lysogenic group of genes Bacteriological
-central culturing in anaerobic media : exotoxin , Tetravalent toxoid (ABE , tetanus toxoid)
- Found in soil, Neurotoxin - attacks centres of brain
-subterminal (tennis - Wilson Blair media depends on Pentatoxoid
contaminated vege , - Blocks ACh release Bacterioscopical not beneficial
racket appearance) - Chopped meat glucose type of - polyvalent 5 toxoids (ABE + 2 gas gangrene
food and animals - Flexoid paralysis
media C.Botulinum toxoids)
The vegetative form grows Serological Hexatoxoid
and produces toxin in - neutralization test (in vivo) the ability of - Polyvalent 6 toxoids (3 botulism, 2 gas
anaerobic condition botulism antitoxin to neutralize botulism toxin gangrene and 1 tetanus toxoid)
Epidemiology injected in mice ( a form of biological test) Proper sterilization when packing food
Pathogenesis Clinical Findings -passive hemagglutination test.
Adequately cooked food
Spores, widespread in soil, contaminate vege and - R-IHA, RIA ( in vitro)
From Gut Weakness and paralysis Erythrocyte diagnosticum with antibody A
meats
Via bloodstream Diplopia Erythrocyte diagnosticum with antibody B
Canned @ vacuum-packed food without adequate
To peripheral NS in nerve synapse blocks Dysphagia Polyvalent erythrocyte diagnosticum with
sterilization
release of ACh Resp. muscle failure antibody ABE
2 types of clinical form :
- wound botulism (locally) - PCR for diagnosis
- infant botulism ( grows in gut)