Beruflich Dokumente
Kultur Dokumente
Taxonomy and and tinctorial (models) structure Modes of virus Specimens and Method of Prophylaxis
Classification properties transmission Examination
Infectious ~ enveloped ~ linear double ~ cell ~ viral capsid ~ transmitted (a) Specimen ~ immunity
mononucleosis stranded DNA culture An by exchange of ~ saliva response to EBV
(Herpes viruses) ~ icosahedral (used in saliva of IgM antibody
diagnosis test) (during kissing) (b) Hematogenic approach to VCA, IgG for
F: Herpetoviridae ~ 120 200 ~ 30% abnormal lymphocytes VCA is for life
G: nm ~ early An ~ blood seen on smears (IgG reveal
gammaherpesviri (produce prior transmission prior infection)
dae to viral DNA (rare) (c) Immunology approach
synthesis) ~ life time
~ Epstein Barr **Heterophil antibody test immunity
virus (EBV) ~ nuclear An - Early diagnosis usually against 2nd
(EBNA) +ve by 2nd week of episode is
(HV-4) infection (not useful for based on
~ lymphocyte detection of prior Antibody to viral
determined mb infection) mb An
An - Monospot test
Detect heterophil ~ heterophil
~ viral mb An antibody Antibody
(neutralization More sensitive, (detect by test
activity against specific and cheaper with An diff from
it) than tube the an that
agglutination test induce them)
** EBV Antibody specific test
- IgM Viral Capsid An(VCA) Treatment
antibody respond can be ~ no antiviral
used to detect early therapy
illness
- IgG VCA antibody detect ~ inc dose
by prior infection acylovir in life
threatening EBV
(d) Fluorescent Antibody
staining of nuclear smear Prevention
~ non specific
~ no vaccine
Pathogenesis: - herpesviridae
Viruss name and Morphology and Genome Culturing Antigenic Routes and Modes of Microbial Diagnosis, Treatment and
Taxonomy and tinctorial (models) structure virus transmission Specimens and Method Prophylaxis
Classification properties of Examination
Chicken Pox ~ enveloped ~ linear ~ in cell ~ single ~ by respiratory (a) Specimen Immunity
(Varicella) double culture serotype droplets ~ lesion ~ lifelong for
~ Isosahedral stranded DNA Varicella but
Zoster (Shingles) capsid ~ by direct contact (b) Tzanck smear zoster can occur
of lesion (highly ~ presumptive despite of
F: Herpesviridae ~ 120-200nm contagious in diagnosis immunity to
Subfamily: children) varicella
alphaherpesvirida (c) Virological
e ~ isolation of virus in Treatment
cell culture and ~ no antiviral
~ Varicella-zoster identification with treatment
virus (VZV) specific antiserum
(serological diagnostic) ~ in adult if
(HV-3) severe, treat
~ antibody titer inc with acyclovir
used for diagnose of (dec the
varicella but less duration and
efficient in zoster severity
symptoms)
~ disease
caused by
acyclovir
Prevention: resistance strain
(1) Acyclovir of VZV are
(2) Varicella-zoster Ig treated with
(which contain high foscarnet
level of antibody to
the virus) ~ no drugs cure
(3) Vaccine (live latent state
attenuated VZV)
~ one dose (1-
12yrs)
~ two doses
( teenagers who has
no disease yet)
** do not give to
immunocompromised
and pregnant women
*** dried crusts of skin lesions du not have active virus particles
Viruss name and Morphology and Genome Culturing Antigenic Routes and Modes of Microbial Diagnosis, Treatment and
Taxonomy and tinctorial (models) structure virus transmission Specimens and Method Prophylaxis
Classification properties of Examination
Herpes simplex ~ enveloped ~ linear ~ in cell ~ HSV-1 by saliva (a) Specimen Treatment
virus type 1 and double culture ~ lesion ~ Acyclovir
type 2 ~ Isosahedral stranded DNA ~ HSV-2 by sexual
capsid contact (b) Virological Prevention
(HSV-1, HSV-2) ~ isolation of virus ~ non specific
~ 120-200nm ~ typical cytopathic
F: herpesviridae effect occurs in 1-3 ~ long term
Subfamily: days administration
Alphaherpesvirus ~ identified by IF of drugs
antibody staining ( acyclovir,
~ELISA valacyclovir,
HSV-1 famciclovir) can
** (c) Rapid diagnosis suppress
gingivastomatitis ~ Tzanck smar, cell recurrences
** Herpes labialis stain with Giemsa stain,
** presence of
keratoconjunctiviti multinucleated giant
s cells
** encephalitis ~ PCR
** Herpetic
whitlow of skin (d) Serological
(finger or hand) ~ neutralization test
** disseminated for primary infections
infections (e.g. (no use for recurrent
pneumonia, infections)
esophagitis)
HSV-2
**genital herpes
** neonatal
herpes
**aseptic
meningitis
Viruss name and Morphology and Genome Culturing Antigenic Routes and Modes of Microbial Diagnosis, Treatment and
Taxonomy and tinctorial (models) structure virus transmission Specimens and Prophylaxis
Classification properties Method of
Examination
Measles ~ enveloped ~ single ~ in tissue ~ single ~ by respiratory (a) Specimens ~ lifelong
stranded culture serotype droplets (coughing, ~ cough plate, saliva immunity although
F: ~ helical sneezing after the IgG antibody play a
Paramyxoviridae nucleocapsid ~ -ve sense ~ rash appear) (b) Virological role in neutralizing
Subfamily: Hemagglutini ~ isolated in cell virus in uremia
Paramyxovirinae ~ 150nm ~ RNA virus n ~ from maternal to culture stage
(not fetus
G: Morbilivirus ~ envelop segmented) ~ fusion (c) Serological ~ cell mediated
contains two protein ~ have 4 folds of
** slow viral types of spike ~ contain rising in antibody
infection ** with RNA titer (used to Treatment
hemagglutinatin dependent diagnose difficult ~ no antiviral
g activity RNA case) therapy
** with cell polymerase
fusing and Prevention
hemolytic ~ live attenuated
activities vaccine (with
mumps and rubella
vaccine)
**MMR vaccine
~ to age of 15
months
maternal antibody
in child neutralize
the virus and
reduce the
immune response
~ booster dose is
recommended
Clinical findings:
~ fever
Viruss name and Morphology and Genome Culturing Antigenic Routes and Modes of Microbial Diagnosis, Treatment and
~ malaise and
Taxonomy tinctorial (models) structure virus transmission Specimens and Method Prophylaxis
Classification properties of Examination
~ cough
~ conjunctivitis
~ photophobia
~ anorexia
Mumps ~ enveloped ~ single ~ in cell ~ ~ by respiratory (a) Specimen ~ life long
stranded culture Hemagglutini droplets ~ droplets of immunity
F: ~ helical n respiratory (maternal
Paramyxoviridae ~ -ve sense ~ direct contact ~ saliva, spinal fluid Antibody for 1st
Subfamily: ~ capsid RNA ~Neurominida 6 months)
Paramyxovirinae se (b) Virological
G: Rubella virus ~ 150nm ~ non ~ isolation in cell Treatment
segmented ~ fusion culture from saliva, ~ no antiviral
~ have two protein spinal fluid or urine
types of envelop Prevention
spikes ~ internal (c) Serological ~ live
** with capsid protein ~ have 4 folds rising in attenuated
Hemagglutinin is S(soluble) antibody titer in either vaccine
and antigen HAI or CF test is
neuraminidase **detected by diagnostic ~ no Ig
** with cell CF
fusing and ~ CF test that asay both
hemolytic S and V (viral) An is
activity used
Clinical signs
~ 5 15 years children
~ anorexia
~ headache
~ malaise
~ if pregnant women
expose to virus, presence
of IgM recent infection 1:8
titer IgG indicator
immunity and
Clinical findings consequence protect of
fetus
~ fever