Beruflich Dokumente
Kultur Dokumente
Title : SALIVA
Day/Date : Monday, November 20th 20156
Name : Khairul Ashar Chairan
ID : 1413442009
Class/Group : ICP A/ II (Two)
Partnerts : 1. Andi Tenri Tayu
2. Jumriana Jufri
3. Yuli Ratnadillah
Assistant : Marwah Karim
A. OBJECTIVE OF EXPERIMENT
At the end of this experiment in the hope students can:
a) Knowing the mucin test
b) Knowing the saliva test compounds inorganic compounds
c) Knowing thiocyanate test
d) Knowing test the effect of temperature on the activity of ptyalin
e) Knowing test ptyalin
f) Knowing the pH determination test is suitable for employment saliva
g) Knowing the effects of compounds that inhibit / destroying the activity of
bacteria in salivary amylase.
B. APPARATUS AND CHEMICALS
1. Apparatus
a. Measuring cup 10 ml and 20 ml 2 pieces
b. Beaker 50 ml, 250 ml and 600 ml 1 piece
c. Test tube 10 pieces
d. Test tube rack 1 piece
e. 100oC thermometer 1 piece
f. Funnel usual 1 piece
g. Pipette 20 pieces
h. Tripod and gauze asbestos 1 piece
i. Spiritus 1 piece
j. Stopwatch 1 piece
k. Stir bar 1 piece
2. Chemicals
a. Saliva
b. Distilled water
c. BaCl solution of 25%
d. Solution of NH 4 -Oksalat 4%
e. CH3COOH solution 2 N and 0.1N
f. Starch solution 1%
g. reagent millon
h. reagent benedict
i. reagent molisch
j. Solution I2 0.01 M
k. 0.1 M NaCl Solution
l. Filter paper
m. toluene
n. Solution of concentrated HCl
o. chloroform
p. HgCl solution of 1%
q. Phenol 2%
r. AgNO3 solution
s. HNO3 aqueous solution
t. Phenol solution (pH 5, 7, 9)
u. NaF and 0.1 M FeCl
v. Tissue and lighters
C. WORK PROCEDURES
1. Mucin Test
Millon Benedict Mollish
Reagent Reagent Reagent
2. Thiocyanate test
If red still means there thiocyanate, if
the red color is missing then no
thiocyanate
1 2 3 4
15 mL saliva
Drops with 2 N acetic acid,
forming a precipitate Enter into 4 different reaction
Heated to boiling filtered The filtrate tubes
was inserted
3 mL
Checking ion Cl
1 1
Checking PO43-
2 2
3 3
In each experiment carried out observations of the results are instantaneous, and
also after one hour and then take a good look possibility precipitate formed little
that it is difficult to observe.
Every 5 minutes to take samples and checked tube 4 added salivary that has
in with 2 drops of 0.01 M I2, and record the been heated for 5 minutes
speed of the starch solution in each tube
5,2
8 7,4 6,8 6
Adding I2 and specify in which a tube is missing The addition of acetic acid
the color point is reached so that the mixture becomes
acidic atmosphere
7. Effect compounds that inhibit / destroying the activity of bacteria in salivary
amylase
8 mL
water 5 drops 5 drops
5 drops
toluene chlorofor HgCl2 1%
1 mL liquid salive into 7 reaction tube
m
1 2 3 4 5 6 7 1 2 3
2 mL saliva.
1 2 3 4 5 6 7 1 2 3 4 5 6 7 6 5 4
in water 38oC
6 7 each tube
1 2 3 4 5
halved I2 Benedict
solution Solution