EXTRACTION OF LYCOPENE FROM

TOMATO

A SEMINAR REPORT

SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENT FOR

THE AWARD OF DEGREE OF
BACHELOR OF ENGINEERING
(CHEMICAL ENGINEERING)

SUBMITTED BY
KRISHNAPRIYA NAIR
UNDER THE GUIDANCE OF

Prof.A.G.THOKAL

DEPARTMENT OF CHEMICAL ENGINEERING

BHARATI VIDYAPEETH COLLEGE OF ENGINEERING
(Affiliated to University of Mumbai)
SECTOR- 7, C.B.D BELPADA, NAVI MUMBAI 400614.
(2016-2017)
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 BHARaTI VIDYAPEETH COLLEGE OF ENGINEERING,

NAVI MUMBAI

Certificate
This is to certify that KRISHNAPRIYA NAIR has successfully completed
the SEMINAR work entitled EXTRACTION OF LYCOPENE FROM
TOMATO during the prescribed period in the academic year 2016-17. This
Seminar Report is submitted in the partial fulfillment of the requirement for
the Award of Degree of
BACHELOR OF ENGINEERING
IN
CHEMICAL ENGINEERING

Prof.A. G. Thokal Dr. M .V. Bagal

Seminar Guide Seminar Coordinator

Prof.(Dr.) S. P. Shingare Principal

Head B.V.C.O.E., Navi Mumbai
Department of Chemical Engineering

Examiner 1_________________________
Examiner 2_________________________

Date:

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 Acknowledgement
I would first of all like to thank and express my sincere gratitude to
Prof.A.G.Thokal, Seminar Guide, Department of Chemical
Engineering, B.V.C.O.E., Navi Mumbai for his constant support and
encouragement during my seminar work. I have high regards for his
guidance, motivation and their dynamic presence as my guide.
I take this opportunity to express my sincere gratitude to the Dr. M.V.
Bagal, Seminar Coordinator and Dr. S. P. Shingare, Head,
Department of Chemical Engineering for their valuable help and
suggestions during this work. I sincerely acknowledge all help provided
by the entire staff and my colleagues of Department of Chemical
Engineering, B.V.C.O.E. Navi Mumbai, for providing me with valuable
and practical information relevant for the successful completion of this
seminar work.

KRISHNAPRIYA NAIR

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 BHARATI VIDYAPEETH COLLEGE OF ENGINEERING, NAVI
MUMBAI

INSTITUTE MISSION
Social Transformation through Dynamic Education

DEPARTMENT OF CHEMICAL ENGINEERING

VISION

The chemical engineering department seeks to prepare chemical engineers to pursue their goals
as successful careers of Entrepreneurs and Eminent Researchers which benefits the public
welfare through its service that is rigorous and challenging.

MISSION

1. Excel in engineering practice, research, and management in industries.

2. Expands the knowledge through its scholarly pursuits.
3. Think critically and creatively, especially about the use of technology to address local and
global problems.
4. To develop technology to serve social needs.

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CONTENTS
Page No
LIST OF FIGURES i
LIST OF TABLES ii
CHAPTER 1: INTRODUCTION 1
CHAPTER 2: LITERATURE SURVEY 3

CHAPTER3: LYCOPENE DESCRIPTION 7

CHAPTER4: EXTRACTION PROCESS 13

CHAPTER5: CONSTRUCTION AND WORKING 16
5.1 OPERATION 18
5.2 ANAYLSIS OF LYCOPENE 20
5.3 RESULTS AND DISCUSSION 23
CHAPTER6: CONCLUSION 24
CHAPTER 7: APPLICATIONS 26

REFERENCES 29

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LIST OF FIGURES

TITLE Page No.

Figure 3.1 Content of carotene and lycopene in the blanched and non-blanched 8
peels and flesh
Figure 3.2 Structure of Lycopene 12
Figure 4.1 Soxhlets original illustration of his automated batch extractor 15
Figure 5.1 Soxhlet Extractor 17
Figure 53 TLC Silica plate with drop of lycopene 20
Figure 5.4 Chromatogram of extract with ethyl acetate 21
Figure 5.5 Chromatogram of extract with dichloromethane 22
Figure 5.6 Chromatogram of extract with petroleum ether 22

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LIST OF TABLES

TITLE Page No.

Table 3.1 Tomato Composition given in USDA FOOD Composition 9

Data,value per 100 g
Table 3.2 Lycopene content of common tomato-based food 10
Table 5.1 Boiling point of solvents used 18
Table 5.2 Retention time and peak area of lycopene 22
Table 5.3 Yield calculated on lycopene concentration 23