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jssj

Tiempo en
minutos
1
5
10
15
30

vel inicial
tiempo 0 1 5
concentracion 0 0.05 0.23

1. Vel inicial 0.0457 (mol/ml)/min

2. Actividad de la b-glucosidasa vel inicial * volumen tota


0.0457
R// 2.285 (mol glucosa/min
1.2

Concentracin de 1
f(x) = 0
glucos en mol/ml R = 0
0.8
0.05
0.6
0.23 Concentracion (mmol/ml)
0.38 0.4

0.52 0.2
f(x) = 0
1.03 0 R = 0.9
0 5

el inicial * volumen total


5ml celobiosa
44ml sln buffer
1ml enzima
50 total
Chart Title
1.2

1
f(x) = 0.033026835x + 0.0390726125
0.8 R = 0.9973355794 Concentracin de glucos en mol/ml
Linear (Concentracin de glucos en
0.6 mol/ml)
mmol/ml)
0.4 vel inicial
Linear (vel inicial )
0.2
f(x) = 0.0457142857x + 0.0019047619
0 R = 0.9996745851
0 5 10 15 20 25 30 35

Tiempo (Min)
jssj

lineweaber-burk
t(min) Cs(mol/L) v(mol/L.min) 1/v
0 0.025 0.86 1.1627907
3 0.0227 0.79 1.26582278
6 0.0184 0.7 1.42857143
9 0.0135 0.65 1.53846154
12 0.0125 0.58 1.72413793
15 0.0073 0.46 2.17391304
20 0.0046 0.41 2.43902439
25 0.00204 0.2 5
30 0.00189
40 0.00185

lineweaber-burk
6

5
f(x) = 0.0081708463x + 0.9302592207
4 R = 0.9888050234
3 1/s
Axis Title
Linear ( 1/s)
2

0
0 100 200 300 400 500 600

Axis Title
lineweaber-burk Eadie Hofstee Lagmuir
1/s v v/s s/v s
40 0.86 34.4 0.02906977 0.025
44.0528634 0.79 34.8017621 0.02873418 0.0227
54.3478261 0.7 38.0434783 0.02628571 0.0184
74.0740741 0.65 48.1481481 0.02076923 0.0135
80 0.58 46.4 0.02155172 0.0125
136.986301 0.46 63.0136986 0.01586957 0.0073
217.391304 0.41 89.1304348 0.01121951 0.0046
490.196078 0.2 98.0392157 0.0102 0.00204
0.00189
0.00185

Eadie Hofstee
1

0.8
f(x) = - 0.0082841924x + 1.0492827829
0.6 R = 0.8965302778
1/s v/s
Linear ( 1/s) Axis Title
0.4 Linear ( v/s)

0.2

0
30 40 50 60 70 80 90 100 110

Axis Title
0 0.025 0.86
3 0.0227 0.79
6 0.0184 0.7
9 0.0135 0.65
12 0.0125 0.58
15 0.0073 0.46
20 0.0046 0.41
25 0.00204 0.2
30 0.00189
40 0.00185

Lagmuir
0.035
0.03
f(x) = 0.8786159528x + 0.008816407
0.025 R = 0.9718398317
0.02
v/s s
Linear ( v/s) Axis Title 0.015 Linear ( s)
0.01
0.005
0
0 0.005 0.01 0.015 0.02 0.025 0.03

Axis Title
s
Linear ( s)
jssj
lineweaber-burk
S(mg/ml) r 1/v 1/s
12.56 101 0.00990099 0.07961783
11.24 98.2 0.01018330 0.08896797
9 92.4 0.01082251 0.11111111
8.12 90 0.01111111 0.12315271
6.33 82.7 0.01209190 0.15797788
5.61 79.1 0.01264223 0.17825312
4.28 70.9 0.01410437 0.23364486
3.56 65 0.01538462 0.28089888
2.34 51.7 0.01934236 0.42735043
1 28.8 0.03472222 1.00000000

lineweaber-burk
0.04000000
0.03500000
f(x) = 0.0269271817x + 0.0078114509
0.03000000
R = 0.999986653
0.02500000
0.02000000 1/s
Axis Title
0.01500000 Linear ( 1/s)
0.01000000
0.00500000
0.00000000
0.00000000 1.00000000 2.00000000

Axis Title
Eadie Hofstee Lagmuir
v v/s s/v s
101 8.04140127 0.12435644 12.56
98.2 8.7366548 0.11446029 11.24
92.4 10.2666667 0.0974026 9
90 11.0837438 0.09022222 8.12
82.7 13.0647709 0.07654172 6.33
79.1 14.0998217 0.07092288 5.61
70.9 16.5654206 0.06036671 4.28
65 18.258427 0.05476923 3.56
51.7 22.0940171 0.04526112 2.34
28.8 28.8 0.03472222 1

Eadie Hofstee
120

100
f(x) = - 3.4632382219x + 128.2786803538
80 R = 0.9998307023
1/s 60 v/s
Linear ( 1/s)
Axis Title
Linear ( v/s)
40

20

0
5 10 15 20 25 30

Axis Title
Lagmuir
0.14
0.12
f(x) = 0.0077677898x + 0.027157617
0.1 R = 0.9999520256
0.08
v/s s
Axis Title 0.06
Linear ( v/s) Linear ( s)
0.04
0.02
0
0 2 4 6 8 10 12 14

Axis Title
s
Linear ( s)
jssj

benzoato de metilo sin inhibicion


S (mol/L) V (nmol/min)
3.7 10
13 30
39 63
79 87
230 116
400 122

Sin inhibicion

Metodo grafico
S V
3.7 10
13 30
39 63
79 87
230 116
400 122

a.
140

120

100

80
Axis Title 60

40

20

0
0 50 100 150 200 250 300 350 400 450

Axis Title
Vmax 120
Km 40

B.

benzoato de metilo sin inhibicion


S (mol/L) V (nmol/min)
3.7 10
13 30
39 63
79 87
230 116
400 122

GRAFICA. LINEWEAVER-BURK

benzoato de metilo sin inhibicion


1/S 1/V
0.2702702703 0.1
0.0769230769 0.0333333333
0.0256410256 0.0158730159
0.0126582278 0.0114942529
0.0043478261 0.0086206897
0.0025 0.0081967213

0.2
f(x)
f(x)==0.7165559777x
1.7564195672x++0.0038957836
0.0043234238
0.18 R
R==0.9963064803
0.9968335177

0.16

0.14

0.12

0.1
1/V f(x) = 0.3433682403x + 0.0071334618
R = 0.9999849754
0.08

0.06

0.04

0.02

0
-0.02 0.03 0.08 0.13 0.18 0.23 0.28

1/S
0.06

0.04

0.02

0
-0.02 0.03 0.08 0.13 0.18 0.23 0.28

1/S
inhibicion 1 inhibicion 2
acetofenona 3,7 x10-4M acetofenona 1,58X10-3M
V (nmol/min) V (nmol/min)
5
20
45 20
70 40
102 80
122 110

lineweaber-burk Eadie Hofstee


1/s 1/v v/s v
0.2702702703 0.1 2.7027027 10
0.0769230769 0.0333333333 2.30769231 30
0.0256410256 0.0158730159 1.61538462 63
0.0126582278 0.0114942529 1.10126582 87
0.0043478261 0.0086206897 0.50434783 116
0.0025 0.0081967213 0.305 122

lineweaber-burk
0.12

0.1
f(x) = 0.3433682403x + 0.0071334618
0.08 R = 0.9999849754

0.06
Axis Title
0.04

0.02

0
300 350 400 450 0 0.05 0.1 0.15 0.2 0.25 0.3

Axis Title
Vmax 140.84507
Km 48.366197

inhibicion 1 inhibicion 2
acetofenona 3,7 x10-4M acetofenona 1,58X10-3M
V (nmol/min) V (nmol/min)
5
20
45 20
70 40
102 80
122 110

inhibicion 1 inhibicion 2
acetofenona 3,7 x10-4M acetofenona 1,58X10-3M
1/V 1/V
0.2
0.05
0.0222222222 0.05
0.0142857143 0.025
0.0098039216 0.0125
0.0081967213 0.0090909091

sin inhibicion 1/V


Linear (sin inhibicion 1/V)
acetofenona 3,7 x10-4M
Linear (acetofenona 3,7 x10-4M)
acetofenona 1,58X10-3M
Linear (acetofenona 1,58X10-3M)

0.18 0.23 0.28


acetofenona 1,58X10-3M
Linear (acetofenona 1,58X10-3M)

0.18 0.23 0.28


Lagmuir
s s/v
3.7 0.37
13 0.43333333
39 0.61904762
79 0.90804598
230 1.98275862
400 3.27868852

Eadie Hofstee
140
120
f(x) = - 47.1305080736x + 138.3874253584
100 R = 0.9992324467
80
1/v
Linear ( 1/v)
Axis Title 60
40
20
0
25 0.3 0 0.5 1 1.5 2 2.5

Axis Title
Vmax 138.39
Km 46.131

R// Es inhibicion competitiva


Hofstee Lagmuir
3.5

138.3874253584 3 f(x) = 0.007317065x + 0.3327524172


R = 0.999759271
2.5

v 2

Linear ( v) Axis Title 1.5


1
0.5
0
2 2.5 3 0 50 100 150 200 250 300 350 400 450
e Axis Title
Vmax 136.986301
Km 45.5890411
Lagmuir

7317065x + 0.3327524172
759271

s/v
Linear ( s/v)

150 200 250 300 350 400 450

Axis Title
jssj

sin inibicion Inhibicion 1


S(mg/ml) r S r
12.56 101 10 77
11.24 98.2 7.7 71.4
9 92.4 5.26 62.5
8.12 90 4.55 58.9
6.33 82.7 3.33 51.4
5.61 79.1 2.04 38.9
4.28 70.9 1.89 37
3.56 65 1.67 34.2
2.34 51.7
1 28.8

Graficas Linewaver-burk

sin inibicion Inhibicion 1


1/s 1/v 1/s 1/v
0.07961783 0.0099009901 0.1 0.01298701
0.08896797 0.0101832994 0.12987013 0.0140056
0.11111111 0.0108225108 0.19011407 0.016
0.12315271 0.0111111111 0.21978022 0.01697793
0.15797788 0.0120918984 0.3003003 0.01945525
0.17825312 0.012642225 0.49019608 0.02570694
0.23364486 0.0141043724 0.52910053 0.02702703
0.28089888 0.0153846154 0.5988024 0.02923977
0.42735043 0.0193423598
1 0.0347222222

sin inibicion
0.04
0.035
f(x) = 0.0269271817x + 0.0078114509
0.03
R = 0.999986653
0.025
0.02
Axis Title
0.015
0.01
0.005
0
0 0.2 0.4 0.6 0.8 1 1.2

Axis Title
Axis Title
0.015
0.01
0.005
0
0 0.2 0.4 0.6 0.8 1 1.2

Axis Title

Vmax 128.21
Km 3.45

Tipo de inhibicion

con alfa dextrina


0.05

0.04

0.03

1/V 0.02

0.01

1.21430643318377E-017
0 0.2 0.4 0.6 0.8 1 1.2
-0.01

1/S

R// Inhibicion competitiva


Inhibicion 2
S r
33.3 116
20 109
12.9 102
10 85.5
6.06 71.5
3.64 55.6
2.82 47.6
1.84 35.6
1.6 32.2
1.43 29.5

Inhibicion 2
1/s 1/v
0.03003003 0.00862069
0.05 0.00917431
0.07751938 0.00980392
0.1 0.01169591
0.1650165 0.01398601
0.27472527 0.01798561
0.35460993 0.0210084
0.54347826 0.02808989

Inhibicion
0.035
0.03
f(x) = 0.0325340303x + 0.0097715194
0.025
R = 0.9999421889
0.02
Axis Title 0.015
0.01
0.005
0
1.2 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7

Axis Title
Axis Title 0.015
0.01
0.005
0
1.2 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7

Axis Title

Vmax 102.04
Km 3.32

con maltosa
0.05

0.04

0.03

1/V 0.02

0.01

1.21430643318377E-017
.8 1 1.2 -0.1 0.1 0.3 0.5 0.7 0.9 1.1
-0.01

1/S

R// Inhibicion no competitiva


Inhibicion 2
0.03

0.025 f(x) = 0.038248127x + 0.0074180591


R = 0.9980261048
0.02

0.015
Axis Title
0.01

0.005

0
0.6 0.7 0 0.1 0.2 0.3 0.4 0.5 0.6

Axis Title
Axis Title
0.01

0.005

0
0.6 0.7 0 0.1 0.2 0.3 0.4 0.5 0.6

Axis Title

Vmax 135.14
Km 5.16

maltosa

0.3 0.5 0.7 0.9 1.1 1.3 1.5

1/S

R// Inhibicion no competitiva


0.5 0.6
0.5 0.6