Beruflich Dokumente
Kultur Dokumente
CHM 5570
Preformed 02.19.2016, 02.26.2016
Turned in 03.08.2016
The use of artificial spices that mimic cinnamon is prolific and ubiquitous in the US market, the
most common being cassia. The purpose of this experiment is to determine whether an unknown
solution of cinnamon extract contained actual cinnamon or an analogue molecule that mimics
cinnamons unique smell and taste. This lab will consist of multiple analytical techniques to
determine the presence or absence of certain molecules.
Using a variety of techniques that includes gas chromatography (GC) in conjunction with a mass
spectrometry (MS), we will create an index that will comprise all ingredients found in both pure
cinnamon, cassia, and aggregate forms. An index will be created and used to analyze locally
purchased produced labeled as containing cinnamon, using GC and MS confirmation. By
alternating between samples containing cinnamon compounds and a sample containing
hydrocarbons (octane to eicosane) the order at which various compounds in the sample are eluted
can be determined. The order of elution will remain constant for the duration of the experiment
and will help identify compounds in unknown samples. Cinnamon and cassia differ in several
areas; cinnamon is characterized by the presence of eugenol and benzyl benzoate as minor
components that are absent in cassia GC. For this experiment a local cinnamon extract will be
tested for any indications that the extract isnt pure cinnamon. Analyte indicative of cassia
contain Coumarin and -cadinene that are either absent or only trace amounts detected with
cinnamon. In addition, 2-methoxycinnamaldehyde, Cinnamyl acetate, Cinnamyl alcohol,
cinnamic acid, benzaldehyde, 3-phenylpropanal, and 2-phenylethanol are common to both and
will also be used to identify the unknown solution.
Gas chromatography is a notable analytical method that separates compounds from complex
samples with a liquid stationary phase and a gas
mobile phase. The rates and orders at which
compounds are eluted from the column depends
on the molecules various chemical and physical
properties, and how said properties interact with
the stationary phase. For part A, the GC is used
in conjunction with a flame ionization detector,
a detector only applicable for hydrocarbon or
organic containing compounds as it works by
carbons ability to form cations and electrons
upon pyrolysis. The electrons generate a current Figure 1: A GC-MS schematic [2]
between electrodes that are adjacent to the flame, the increase in current appears as a peak on the
chromatogram.
For Part B, to ensure accuracy a Gas chromatography Mass spectrometry instrument was used
which combines various features of GC and MS to determine the identities of various substances
within a test sample. The mass spectrometer used electron ionization (EI) as a form of ionization.
In EI, the molecules enter the MS and are subsequently bombarded by electrons that causes the
molecule to fragment in a characteristic and reproducible manner. [1] This form of ionization is
considered a hard ionization technique and results in a myriad of fragments with low mass to
charge ratios (m/z). By comparing the pattern of molecular fragmentation to online libraries the
identities of the molecules can be confirmed.
Part A
A retention index was established by testing 4 standard solutions over the course of 20 to 30
minutes, a time determined to be sufficient for suitable separation of compounds. In lab it was
found that 20-minute time span was sufficient for full separation of all compounds. The HP5
column contained 5% phenyl methanol. The standards were prepared in a solution of acetonitrile,
alkane solutions in hexanes, ranging from octane to icosane (C8-C20). The initial temperature
(Ti) was set at 40C, with a temperature change (T) of 20C/min, and a final temperature (Tf) of
250C with a four minute hold. The inlet temperature was set to 230C and the column was ran
with nitrogen and air. The FID (Flame Ionization Detection) gas used was
hydrogen. The standards were injected with a 10C syringe rinsed with acetonitrile with a total
volume of 1.0C solution per injection. A spectra of abundance as a function of time is
established. Calculation of the retention index required sequential injections of a standard
mixture of n-alkanes and then standards and extracts, so that approximate times of various
carbon chains can be be used to correctly identify standard elution times. Therefore, GC analysis
was performed by alternating the hexanes solution and the standards in the following order: SM-
2, SM-3, SM-2, SM-4, SM-2. The results of GC alone arent sufficient for confident analysis;
thus the experiment was performed in conjunction with FID mass spectroscopy. The results of
the retention index are found below on table 2 in appendix A and the retention index is found
below.
Part B
Discussion
The fusion of gas chromatography and Mass spectrometry is a very powerful analytical tool that
allowed us to unquestionably determine that the cinnamon extract contained pure cinnamon.
Because most molecules tested only differed by only a carbon or two certain fragmentation
patterns became quickly evident by working out the structures of the ionized molecule as it is
broken down by electrons. By crosschecking online reference patterns, MS in conjunction with
GC allows structure determination with high confidence.
Based on the retention index established, relative peak area, and MS data, the botanical source of
the cinnamon extract is from Cinnamomum Zeylanicum. This can be confirmed by the presence
of eugenol (App.B, Fig10), trace amounts of Coumarin (App.B, Fig12), and an absence of
cadinene. The elution time was consistent with the index established earlier and the MS data was
confirmed via EI indexes online, the EI information for Eugenol can be found in appendix B,
figure 13.
Based on the relationship between the index values and the retention time, a linear relationship
was established with the form [y=122.93x+299.3] where x is the retention time and y is the
retention index value, from this a suitable retention index window of 20.44 minutes to 35.04
minutes would suffice to identify the various individual compounds using the gas
chromatography method outlined in Method A.
From the 2 prominent peaks in Figure 2, the first is what is suspected to be eugenol, could also
possibly be acetonitrile, the substance used to dilute the standard solutions. The source of
contamination may have been from carelessness. The EI spectral data is attached in appendix B,
figure 13. The second prominent peak could also fit the criteria of dimethyl phthalate, and the
data in table 1, appendix B suggests the patterns are very similar. Dimethyl Phthalate is used in a
variety of applications and can be found in many plastic products. It is possible some of the
cinnamon extract became contaminated.
A strange anomaly is present in the data in table 2, App. A, where the I value calculated for
Coumarin and Cinnamyl acetate are out of numerical order, and its possible the compounds
were misidentified in lab when the retention index was made. This obviously had an impact on
the plot of peak vs I value (App. A, Graph 1.), and by reversing the order of Cinnamyl acetate
and Coumarin the r squared value for the linear trend was increased from 0.963 to 0.966.
There are many advantages of using a GC-MS system, but there are a few disadvantages. First,
the length of time required to accrue sufficient data for the retention index is time consuming. In
addition, there are limits on which types of molecules GC-MS is able to analyze, as nonvolatile,
polar and thermally labile compounds are undetectable. Despite this. GC-MS is a powerful tool
capable of separating and identifying all ingredients in a unknown sample.
Appendix A
Table 1.
STANDARD 1
PEAK Time Area MW
1 5.713 5213.4 Benzaldehyde 106.121
2 7.006 5957.8 Linalool 154.13
3 8.609 6132 trans-cinnamaldehyde 132.16
4 8.887 5184.4 Cinnamyl Alcohol 134.17
5 9.258 4841.3 Eugenol 164.2
6 9.893 5214 cinnamyl acetate 176.21
7 10.053 3904.1 coumarin 146.1427
8 10.6 6178 2-Methoxycinnamaldehyde 162.1852
9 12.194 6310.6 benzyl benzoate 212.25
STANDARD 2
8 2.948 164.6 108
9 3.916 206.3 122
10 4.941 235.7 136
11 5.957 255.5 150
12 6.912 272.5 164
13 7.799 281.3 178
14 8.626 301.8 192
15 9.401 324.2 206
16 10.131 344.7 220
17 10.52 352.3 234
18 11.472 385.4 254
19 12.128 434.7 270
20 12.801 415.2 288
STANDARD 3
1 6.963 575.2 linalool 154.13
2 8.842 3196.4 Cinnamyl Alcohol 134.17
3 9.988 2281.4 2-Methoxycinnamaldehyde 162.1852
4 10.614 8749.4 Coumarin 146.1427
STANDARD 4
1 5.693 1845.5 benzaldehyde 106.121
2 8.576 1942.77 cinnamaldehyde 132.16
3 9.251 4412.9 eugenol 164.2
4 9.995 8799.26 cinnamyl acetate 176.21
5 12.238 12710.8 benzyl benzoate 212.25
UNKNOWN
1 5.678 10 benzaldehyde 106.121
2 6.693 147.7 linalool 154.13
3 8.554 115.2 cinnamaldehyde 132.16
4 8.685 105.4 cinn-oh 134.17
5 9.174 7445.5 Eugenol 164.2
6 9.795 164.3 Cinnamyl Acetate 176.21
7 9.838 93 coumarin 146.1427
8 10.046 36.2 2-Methoxycinnamaldehyde 162.1852
9 10.561 43.37 benzyl benzoate 212.25
10 12.137 344.5
Table 2.
Peak Compound Area Time MW Relative areas #carbons I
1 Benzaldehyde 5213.4 5.713 106.121 0.826133807 7 NA
2 Linalool 5957.8 7.006 154.13 0.944094064 10 1203
3 trans-cinnamaldehyde 6132 8.609 132.16 0.971698412 9 1357
4 Cinnamyl Alcohol 5184.4 8.887 134.17 0.821538364 9 1385
5 Eugenol 4841.3 9.258 164.2 0.767169524 10 1425
6 cinnamyl acetate 5214 9.893 176.21 0.826228885 11 1512.14
7 coumarin 3904.1 10.053 146.1427 0.618657497 9 1498.73
8 2-Methoxycinnamaldehyde 6178 10.6 162.1852 0.978987735 10 1557
9 benzyl benzoate 6310.6 12.194 212.25 1 14 1860.4
References
[1] - http://www.massbank.jp/jsp/Dispatcher.jsp?type=disp&id=JP003484&site=10
[2] "Critical Mass: A History of Mass Spectrometry". Chemical Heritage
Foundation. Retrieved 23 January 2015.
[3] - Wiley's Scientific, Technical, and Medical Databases: Home. wiley.com