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A few drops of 0.01 M I2 was added into 1 mL of the sample solution. The
mixture was warmed in a water bath and was observed if there is any change in
color. The mixture was cooled and the result was noted.
Hydrolysis of Polysaccharides
A. Acid Hydrolysis
5 drops of conc. HCl was added to 5 mL of the isolate. It was covered with
marble and boiled in a water bath for 30 minutes.
B. Enzymatic Hydrolysis
C. Phenylhydrazone Test
Thin-layer Chromatography