Giemsa stain is a classic blood film stain for peripheral blood smears and bone marrow

specimens. It can be used for histopathological diagnosis of malaria and some other spirochete
and protozoan blood parasites. It is also used in Wolbachia cell stain in Drosophila
melanogaster.
Calcofluor-white is a special fluorescent stain that binds strongly to structures containing
cellulose and chitin. In plant cell biology research, it is used for the staining of cell walls of both
algae and higher plants.
Malachite green is an organic compound that is used as a dyestuff and controversially as an
antimicrobial in aquaculture. Malachite green is traditionally used as a dye for materials such as
silk, leather, and paper. Although called malachite green, this dye is not prepared from the
mineral malachite - the name just comes from the similarity of color. (The endospore stain is a
differential stain used to visualize bacterial endospores.Endospores are formed by a few
genera of bacteria, such as Bacillus . By forming spores, bacteria can survive in hostile
conditions. Spores are resistant to heat, dessication, chemicals, and radiation.)
Wright's stain is a histologic stain that facilitates the differentiation of blood cell types. It is
classically a mixture of eosin (red) and methylene blue dyes. It is used primarily
to stain peripheral blood smears, urine samples, and bone marrow aspirates which are
examined under a light microscope.
Lugols Iodine Stain is for the detection of intestinal protozoa and helminth ova and larvae.
For fresh, unpreserved fecal samples, a direct wet mount should be prepared to
detect the presence of motile protozoan trophozites. Make sure the wet mount
preparation of the sample has been thoroughly examined before adding Lugols
Iodine, since iodine tends to paralyze the motility of parasitic organisms and may
obscure some parasitic structures.
Prepare a direct smear of the specimen by mixing a small portion (2 mg) of feces
with a drop of sterile physiological (0.85%) saline on a clean glass slide. 3. Place a
coverslip over the sample and examine the wet mount preparation for the presence
of motile protozoa. The organisms are very pale and transparent and are more
easily observed under low light intensity. 4. Once the wet mount has been
thoroughly examined, a drop of Lugols Iodine (working solution) can be placed at
the edge of the coverslip, or a new mount can be prepared using iodine alone.
ZiehlNeelsen stain, also known as the acid-fast stain, It is a special bacteriological stain used
to identify acid-fast organisms, mainly Mycobacteria. species including M. tuberculosis, M.
ulcerans, and M. leprae and nontuberculous mycobacteria (NTM). Ionic bonding is the most
important type of bonding that occurs in histologic staining techniques. It involves electrostatic
attraction between ions of opposite charge, one of which is fixed in the tissue, and the second of
which is in the dye.
Haematoxylin and Eosin Staining
Hematoxylin and eosin is one of the principal stains in histology. Once a tissue specimen has
been processed by a histology lab, and transferred onto a glass slide, it needs to be
appropriately stained for microscopic evaluation. This is because unstained tissue lacks
contrast: all of the fixed materials have a similar refractive index and a similar color. If you
viewed an unstained tissue section under the microscope, everything would appear a uniform
dull grey color.
The staining process therefore makes use of various dyes that stain particular cell components
within tissues, so that you can distinguish different cell parts from each other.Haematoxylin
alone is not technically a dye, and will not directly stain tissues. It therefore needs to be used in
combination with a mordant a compound that helps it link to the tissue. The mordant used is
typically a metal cation, such as aluminium. Haematoxylin in complex with aluminium salts is
cationic and acts as a basic dye. It is positively charged and can react with negatively charged,
basophilic cell components, such as nucleic acids in the nucleus. These stain blue as a result.

Water Based Microscopy Slide Mountant (L&C) 20ml in Dropper Bottle

Water-based & non-toxic, dries rapidly, Suitable for use with immersion oil, Good alternative to
xylene based mountants, Dries clear and preserves specimens A water based microscope slide
mountant based on Lubkin & Carsten's formula. Dries to a clear hard film and requires no fixing
so is suitable for mounting specimens direct from water or for embedding. Suitable for use with
Oil Immersion lenses and our non-toxic Immersion Oil. Packaged in dropper bottle for ease of
use - no pipetting required