Exercises yi 19: Peper chromatography was uséd te identity the amino acids present n'a sare, X. ‘The unknown sample afid Samples of four known amino acids were run under identical concttions, withthe spots of sample being placed near the bottom ofa sist ot fee Peper which was ther dipied into a suitable'solvent: the solvent crept up the paper by apilary attraction. Afior dévelopment to make the spots visible) the chroneiterern was as shown below. Whic8 amino acids are present in.the unknown sample? 1-—solvent front J (level cropt toby 2 " ‘capiliry attraction) |—solvent tiquid level _ (below sample spots) + Bo'ln Figure-44.5(b) the slandatd saripie contained leucine; alaninerglycine, serine, . lysine and aspartic acid."The ‘érder of their rates of travel up a paper under the conditions used is the orde®‘of listing: leucine is the fastest, aspartic acid the slowest. By comparing the location of Spots on the chromatogram, identify the amino acids present in Samples X and ¥. =." 11 Ina paper Hhrometography experiment using a stationary phalse that is more poler than the mobilé phase, which ofthe three'amino acids, alanine, eerie, aspartic ack, -oves the greatest distance and which the leat (na given time)? Explain why B The isoelectric points for some amino acids are given in Table 14.1. For each of the amino acs: : a glycine *b alanine cysteine *d_ysing craw the structure of the predominant species present ina solution of pH: c 120% 60 it 110 23 You may use Table 14.1 for this question. a Inan electrophoresis experimient in @ solution butfered at pH 4.0: “1 Tayards which electrode will each of the amino acids, glycine, valine and Phenylalanine, migrate? Why? ce Ht Of these three amino aeids which will migrate the greatest distance in a given time and which theleast? Explain. else bo | yates Mon Tala b+ Using Figure 14.7 if necessary, towards which electrode in an electrophoresis ‘experiment will aspartic acid migrate in a solution buffered at pH of: 3.0 in 60 Ww 110 © How would the distance moved (in a given time) by aspartic acid in @ butfer at pH 11.0 compare with the distance travelled in a buffer at pH 8.07 Explain.start ne sheet of porous paper 9n 2 plastic support ‘troughs contain butfered electrolyte solution (pH 7.0 here) — voltage —. 3 You may use Table 14.1 for question, i 4 Inan electrophoresis experiment in a solution buted a pH 4.0% | | ToWards which electrode wil each of the amin acids, pein, valine and | phenylalanine, migrate? Why? Of these thee amino acics which wil migrsto the greatest stance ina given time and which the least? Explain » sing Figure 11.7 if necessary, towards which electrode in an electrophoresis Sxperiment will aspartic acid migrate in a solution butfered at pH of." i 10 it 30 80 iv 11.0 © How would the distance moved (in a given time) by aspartic acd ina butfer at PH1-0 compare withthe distance travelled in a buffer at pH 8.07 Expain Baa inorder to identity an amino acid, a chemist ran electrophoresis experiments on {ising butlers at pHs 7-0, 6.0 and 3.0. Te developed electrophoresis sine wa” | 2 (@) below. Which ofthe amino acids in Table 14.1 is @ most likey to be? s2Sa Sxperiment using a butfer of pH 6.0, The result was asin) below. If the ony pssbilties are the amino acids in Table 14.1, ideniy as many spots (abel . FQ, R, S) as you can. (You will net be abla to Identify all of them from this . one experiment.) @ © CFE KEP ao . pH=6.0 > pHs & The chemist repeated the experiment at pH 6.0 and obtained the result in (6) above. If possible identify adctonal spots and suggest possbltes for spss) you are unable to identity. Explain the basis of your identifications,