BC 3600

BC-3600
BC-3300
Auto Hematology Analyzer
Service Manual
Copyright
2008-2011 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights Reserved.
For this Service Manual, the issued Date is 2011-02 (Version: 1.0).
Intellectual Property Statement

SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called Mindray)
owns the intellectual property rights to this Mindray product and this manual. This manual may
refer to information protected by copyrights or patents and does not convey any license under
the patent rights of Mindray, nor the rights of others. Mindray does not assume any liability
arising out of any infringements of patents or other rights of third parties.
Mindray intends to maintain the contents of this manual as confidential information. Disclosure
of the information in this manual in any manner whatsoever without the written permission of
Mindray is strictly forbidden.
Release, amendment, reproduction, distribution, rent, adaptation and translation of this

manual in any manner whatsoever without the written permission of Mindray is strictly
forbidden.
. , are the registered trademarks or trademarks owned by

Mindray in China and other countries. All other trademarks that appear in this manual are
used only for editorial purposes without the intention of improperly using them. They are the
property of their respective owners.
Responsibility on the Manufacturer Party

Contents of this manual are subject to changes without prior notice.
All information contained in this manual is believed to be correct. Mindray shall not be liable for
errors contained herein nor for incidental or consequential damages in connection with the
furnishing, performance, or use of this manual.
Mindray is responsible for safety, reliability and performance of this product only in the
condition that:
all installation operations, expansions, changes, modifications and repairs of this

product are conducted by Mindray authorized personnel;
the electrical installation of the relevant room complies with the applicable national
I
and local requirements;
the product is used in accordance with the instructions for use.
This equipment must be operated by skilled/trained medical professionals.
It is important for the hospital or organization that employs this equipment

to carry out a reasonable service/maintenance plan. Neglect of this may
result in machine breakdown or injury of human health.
Be sure to operate the analyzer under the situation specified in this manual;
otherwise, the analyzer will not work normally and the analysis results will
be unreliable, which would damage the analyzer components and cause
personal injury.
II
Warranty
THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,

EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY OR
FITNESS FOR ANY PARTICULAR PURPOSE.
Exemptions
Mindray's obligation or liability under this warranty does not include any transportation or other
charges or liability for direct, indirect or consequential damages or delay resulting from the
improper use or application of the product or the use of parts or accessories not approved by
Mindray or repairs by people other than Mindray authorized personnel.
This warranty shall not extend to:
any Mindray product which has been subjected to misuse, negligence or accident;
any Mindray product from which Mindray's original serial number tag or product
identification markings have been altered or removed;
any product of any other manufacturer.
Safety, Reliability and Performance

Mindray is not responsible for the effects on safety, reliability and performance of BC-2800Vet,
if:
Assembly operations, extensions, re-adjusts, modifications or repairs are carried out

by persons other than those authorized by Mindray.
Personnel unauthorized by Mindray repairs or modifies the instrument.
Return Policy
Return Procedure
In the event that it becomes necessary to return this product or part of this product to Mindray,
the following procedure should be followed:
1. Obtain return authorization: Contact the Mindray Service Department and obtain a
Customer Service Authorization (Mindray) number. The Mindray number must appear
on the outside of the shipping container. Returned shipments will not be accepted if
the Mindray number is not clearly visible. Please provide the model number, serial
III
number, and a brief description of the reason for return;
2. Freight policy: The customer is responsible for freight charges when this product is
shipped to Mindray for service (this includes customs charges);
3. Return address: Please send the part(s) or equipment to the address offered by
Customer Service department.
Company Contact
Manufacturer: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
Mindray Building, Keji 12th Road South, Hi-tech Industrial Park,

Address:
Nanshan, ShenZhen 518057, P.R.China,
Phone: +86 755 26582479 26582888
Fax: +86 755 26582934 26582500
EC-Representative: Shanghai International Holding Corp. GmbH(Europe)
Address: Eiffestrae 80, 20537 Hamburg Germany
Phone: 0049-40-2513175
Fax: 0049-40-255726
IV
Table of Contents
Copyright I
Intellectual Property Statement I
Responsibility on the Manufacturer Party I
WarrantyIII
Exemptions III
Return PolicyIII
1 Using This Manual 1-1

1.1. Scope 1-2
1.2. Introduction 1-2
1.3. Conventions Used in This Manual 1-3
1.4. Special Terms Used in This Manual 1-3
1.5. Symbols 1-4
1.6. Definitions 1-12
2 Analyzer Structure 2-1

2.1 Product Name 2-1
2.2 System Introduction 2-1
2.3 System Composition 2-7
2.4 Software Structure 2-8
2.5 LIS Connection 2-19
3 Upgrade 3-1
3.1 Software upgrade 3-1
4 Mechanical System 4-1

4.1 Front of the Analyzer 4-1
4.2 Back of the Analyzer 4-3
4.3 Left of the Analyzer 4-4
4.4 Right of the Analyzer 4-6
5 Fluidic System 51
5.1 Fluidic Components and Functions 5-1
5.2 Reagent Consumption 5-2
5.3 Sample Dilution Flowchart 5-3
5.4 Introduction of Fluidic Channels 5-5
5.5 Introduction of Basic Sequence 5-6
6 Hardware System 6-1

6.1 Introduction 6-1
1
Table of Contents
6.2 Data Board 6-5

6.3 Drive Board 6-18
6.4 Power Board 6-25
6.5 USB Interface Board 6-30
6.6 Touchscreen Control Board 6-31
6.7 Volumetric Board 6-32
6.8 Sample Compartment Connection Board 6-34
6.9 Indicator Board 6-35
7 Servicing 7-1
7.1 General 7-1
7.2 Disassembling the Panels 7-2
7.3 Replacing the Hardware parts and Cable connection 7-6
7.4 Replacing the Power Supply Assembly 7-16
7.5 Replacing the Display Assembly 7-17
7.6 Replacing the Touch Screen 7-18
7.7 Replacing the Recorder Assembly 7-19
7.8 Replacing the valve, pumps, and other liquid system parts 7-20
7.9 Replacing the counting bath assembly 7-29
7.10 Replacing the syringe assembly 7-35
7.11 Replacing the sample probe assembly 7-37
7.12 Replacing the sample compartment assembly 7-47
7.13 Replacing the start key 7-56
7.14 Micro-Switch Assembly Replacement 7-57
7.15 Fluidic Support-bracket Assembly 7-58
7.16 Front Panel Detection Assembly 7-59
7.17 Ambient Temperature Sensor Replacement 7-60
7.18 Cap Assembly 7-61
7.19 Gain Adjustment 7-62
7.20 Motor Type, Position and Different Application List 7-64
7.21 The Check List After Material Replacement 7-64
8 Maintaining your analyzer 8-1

8.1 General Guidelines 8-1
8.2 Operation guidance of Quality Control and Calibration 8-2
9 Error Information 9-1

9.1 Error Information and Code 9-1
9.2 Error analysis and processing 9-2
9.3 Error diagnosis assisted by abnormal histogram 9-12
9.4 Error diagnosis assisted by abnormal pulse figure 9-21
10 Appendices A-1
2
1 Using This Manual
This chapter explains how to use this service manual. This manual states in detail the way to
service the BC-3600 and BC-3300 analyzers. Before servicing the BC-3600 and BC-3300,
read and understand the manual carefully for servicing the equipment properly and for your
safety.
This manual is to be used in conjunction with the operator's manuals of BC-3600 and
BC-3300.It does not contain information and procedures already covered in the operator's
manuals of BC-3600 and BC-3300.
Be sure to operate and service the analyzer strictly as instructed in this manual and the
operator's manuals.
1-1
Using This Manual
1.1. Scope
To use this manual effectively, you need the following capabilities:
Comprehensive knowledge of circuit and fluidics;
Comprehensive knowledge of reagents;
Comprehensive knowledge of controls;
Comprehensive knowledge of troubleshooting;
Mastering the way to operate this analyzer;
Using basic mechanical tools and understand related terminology;
Using a digital voltmeter (DVM) and an oscilloscope;
Reading pneumatic/hydraulic schematics and understand related terminology.
1.2. Introduction
This manual comprises 9 chapters and 6 appendices. Refer to the table below to find the
information you need.
If you want to See

learn about the system and software structures of BC-3600 Chapter 2 System Structure
and BC-3300
learn about the installation requirements and the way to Chapter 3 System Installation
upgrade the software of BC-3600 and BC-3300 and Software Upgrade
learn about the mechanical system structure of BC-3600 and Chapter 4 Mechanical System
BC-3300
learn about the fluidic system, reagent dosage, channels and Chapter 5 Fluidic System
basic sequence of BC-3600 and BC-3300
learn about the hardware system and board composition, Chapter 6 Hardware System
adjustment and test points and general troubleshooting
methods of BC-3600 and BC-3300
learn about how to service the BC-3600 and BC-3300 Chapter 7 Servicing
learn about how to maintain the BC-3600 and BC-3300 Chapter 8 Maintenance
learn about how to troubleshoot the common errors of Chapter 9 Troubleshooting
BC-3600 and BC-3300
learn about the main spare parts of BC-3600 and BC-3300 Appendix A List of Spare parts
learn about the main wearing parts of BC-3600 and Appendix B List of Wearing
BC-3300 Parts
learn about the circuit diagram of BC-3600 and BC-3300 Appendix C Circuit Diagram
learn about the schematic diagram of the fluidic system of Appendix D Fluidic Diagram
1-2
Using This Manual
BC-3600 and BC-3300

learn about the function of each valve and pump of BC-3600 Appendix E Pump and Valve
and BC-3300 Function Diagram
learn about the tubing connection of BC-3600 and BC-3300 Appendix F Tubing
1.3. Conventions Used in This Manual
This manual uses certain typographical conventions to clarify meaning in the text:
Format Meaning
all capital letters enclosed in [ ] indicate a key name (either on
[]
the pop-up keyboard or the external keyboard)
letters included in " " indicate text you can find on the screen

of BC-3600 and BC-3300
italic letters indicate titles of the chapters that are referred

to
"Menu"
the " " button on the screen.
All illustrations in this manual are provided as examples only. They may not necessarily reflect
your analyzer setup or data displayed.
1.4. Special Terms Used in This Manual
When you read It means

to press the desired item lightly with your finger; or to
Click
left-CLICK it with the mouse.
to CLICK the desired edit box and use the external keyboard
ENTER or the pop-up keyboard to enter the desired characters or
digits; or to scan the number by using the bar-code scanner.
to move the cursor to the character or digit that you want to
delete by clicking the left button of the mouse or using
[][][Home][End], and then delete the character after the
DELETE
cursor by pressing [Del], or delete the character before the
cursor by pressing [BackSpace] ([] on the upper right part of
the soft keyboard).
to CLICK the down arrow button of the desired box to display
SELECT from
the pull-down list, (and DRAG SCROLL BAR) to browse and
pull-down list
then CLICK the desired item; or to press the keys
(for pull-down list)
([][][PageUp][PageDown]) to browse the current list and
1-3
Using This Manual
press [ENTER] to select the desired item.
1.5. Symbols
You will find the following symbols in this manual.
When you see Then

read the statement below the symbol. The statement is
alerting you to an operating hazard that can cause
personnel injury.
alerting you to a possibility of analyzer damage or unreliable
analysis results.
alerting you to information that requires your attention.

alerting you to a potentially biohazardous condition.
You may find the following symbols on the analyzer, reagents, controls or calibrators.
When you see It means
CAUTION, CONSULT ACCOMPANYING

DOCUMENTS.
BIOLOGICAL RISK
HIGH VOLTAGE
EXERCISE CAUTION WHEN WORKING

AROUND TO AVIOD PRICKING
PROTECTIVE EARTH (GROUND)
ALTERNATING CURRENT
1-4
Using This Manual
FOR IN VITRO DIAGNOSTIC USE
BATCH CODE
USE BY (YYYY-MM-DD)
SERIAL NUMBER
MEASUREMENT AUTHORIZATION
SYMBOL
DATE OF MANUFACTURE
MANUFACTURER
THIS ELECTRO-INFORMATIONAL
PRODUCT CONTAINS CERTAIN
POISONOUS OR HARMFUL SUBSTANCE.
ITS ENVIRONMENTAL LIFT TIME IS 20
YEARS. IT CAN BE USED WHTHIN 20
YEARS, AFTER THAT IT SHALL BE
RECYCLED.
Be sure to observe the following precautions for the safety of patients and operators when you
are servicing the analyzer.
It is important for the hospital or organization that employs this equipment to

carry out a reasonable service/maintenance plan. Neglect of this may result
in machine breakdown or harm to human health.
Never use combustible gas (e.g. anesthetic) or combustible liquid (e.g.

ethanol) around the analyzer. Otherwise, the risk of explosion may exist.
When servicing the analyzer, be sure to turn off the power. Servicing the
analyzer when it is on may bring risk of electric shock or damage to
electronic components.
Connect the analyzer to a socket having sole fuse and protective switch. Do
1-5
Using This Manual
not use the same fuse and protective switch with other equipment (e.g. life
supporting equipment). Otherwise, the equipment failure, over current or
impulse current that occurs at the startup moment may lead to tripping.
To prevent personal injury during maintenance, keep your clothes, hairs and
hands from the moving parts, such as sample probe, clipper and piercer.
Possible mechanical movement of the warned position may lead to personal

injury during the normal operation, removal and maintenance.
Be sure to dispose of reagents, waste, samples, consumables, etc.

according to government regulations.
The reagents are irritating to eyes, skin and mucosa. Wear proper personal
protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory
procedures when handling them in the laboratory.
If the reagents accidentally spill on your skin, wash them off with plenty of
water and if necessary, go see a doctor; if the reagents accidentally spill into
your eyes, wash them off with plenty of water and immediately go see a
doctor.
Improper maintenance may damage the analyzer. Maintain the analyzer

strictly as instructed by the service manual and inspect the analyzer
carefully after the maintenance.
For problems not mentioned in the service manual, contact Mindray

customer service department for maintenance advice.
To prevent personal injury or damage to equipment components, remove

metal jewelry before maintaining or servicing electronic components of the
equipment.
Electrostatic discharge may damage electronic components. If there is a

possibility of ESD damage with a procedure, then do that procedure at an
ESD workstation, or wear an antistatic wrist strap.
When the front cover is open, be sure to close the sample compartment door,
or the sample compartment assembly or the front cover may be damaged.
When transporting the analyzer, the screw at its bottom may scratch your
hands, please exercise caution or wear gloves.
1-6
Using This Manual
This equipment must be operated by skilled/trained medical professionals.
Samples, controls, calibrators and waste are potentially infectious. Wear

proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow
safe laboratory procedures when handling them in the laboratory.
All the analyzer components and surfaces are potentially infectious. Take
proper protective measures for operation or maintenance.
The sample probe tip is sharp and may contain biohazardous materials.
Exercise caution to avoid contact with the probe when working around it.
Figure 1-1
1-7
Using This Manual

Warning, potential biohazardous risk.
Make sure the analyzer is properly grounded.

To avoid electric shock, disconnect power cord prior to removing or replacing fuse.
Use fuse only with the type and rating specified.
1-8
Using This Manual
Figure 1-2
Warning, the sample probe is sharp and may contain biohazardous substance. Exercise
caution to avoid contact with the probe when working around it.
1-9
Using This Manual
Figure 1-3

Warning, make sure the protective cover is properly installed before operation.

Warning
Do not put your hands close to the sampling assembly when the analyzer is running.
Warning, the sample probe is sharp and may contain biohazardous substance.
Exercise caution to avoid contact with the probe when working around it.
Warning, the sample probe is sharp and may contain biohazardous substance. Exercise
1-10
Using This Manual
caution to avoid contact with the probe when working around it.
Figure 1-4

Warning, risk of electric shock.
1-11
Using This Manual
1.6. Definitions
There are two sampling mode: open vial mode and closed tube mode.
Open vial mode
The operator presents the tube or open vial evacuated collection tube containing blood sample
to the sample probe, and press the "Aspirate" key to collect sample.
The analyzer of which the sampling mode is open vial is called open vial analyzer.
Closed tube mode
The operator puts the capped tube into the sample compartment, the analyzer collects sample
by piercing through the tube cap.

The analyzer of which the sampling mode is closed tube is called closed tube analyzer.
1-12
2 Analyzer Structure
2.1 Product Name

Name: Auto Hematology Analyzer.
Models: BC-3600, BC-3300.
2.2 System Introduction

BC-3600 and BC-3300 are in-vitro diagnosis equipments with the functions of blood cell
counting, WBC 3-differential and measurement of HGB concentration under proper working
environment. The purpose of the analyzers is to identify the normal patient, with all normal
system-generated parameters, and to flag or identify patient results that require additional
studies.
Scope: the products can be applied for clinical diagnosing reference and scientific
research.
Environment of use: the analyzers shall be used in medical laboratories with

standardized management; they can not be used as portable equipment.
Operator: the operators must be skilled/trained medical professionals.
2.2.1 System Configuration

The whole system includes the analyzer, accessories and reagents; users can choose to
configure data management software, scanner, printer or PC.
2.2.2 Electrical Parameters

Table2-1 Power supply of the analyzer
Voltage (100V-240V)10%
Input Power 180 VA
Frequency 50/601Hz
Only install fuses of specified specification on the analyzer.
Specification of the fuse: 250V T3.15AH
2-1
Analyzer Structure
2.2.3 Dimension and Weight
Height
Depth
Width
Figure 2-1Dimensional sketch map
Table2-2Dimension and weight
Width (mm) Depth (mm) Height (mm) Weight (Kg)

395 450 445 (without foot) 28
2.2.4 Throughput
The throughput of open vial analyzers under whole blood mode is no less than 60 samples per
hour; the time needed for the analysis of one sample is no more than 60s (the sample dilution
time is not included for predilute mode).
The throughput of closed tube analyzers under whole blood mode is no less than 60 samples
per hour; the time needed for the analysis of one sample is no more than 60s (the sample
dilution time is not included for predilute mode).
2.2.5 Parameters
The analyzer determines 21 parameters (WBC, RBC, PLT, HGB, etc.) and 3 histograms
(WBC, RBC and PLT) of blood samples.
Table 2-3Table of parameters
English name Abbreviation

White Blood Cell count WBC
Lymphocyte number Lymph#
Mid-sized Cell number Mid#
Granulocyte number Gran#
Lymphocyte percentage Lymph%
2-2
Analyzer Structure
Mid-sized Cell percentage Mid%

Granulocyte percentage Gran%
Red Blood Cell count RBC
Hemoglobin concentration HGB
Mean Corpuscular Volume MCV
Mean Corpuscular Hemoglobin MCH
Mean Corpuscular Hemoglobin Concentration MCHC
Red Blood Cell Distribution Width Coefficient of RDW-CV
Variation
Red Blood Cell Distribution Width Standard RDW-SD
Deviation
Hematocrit HCT
Platelet count PLT
Mean Platelet Volume MPV
Platelet Distribution Width PDW
Plateletcrit PCT
Platelet Larger Cell Ratio P-LCR
Platelet Larger Cell Count P-LCC
Table2-4Table of histograms
White Blood Cell Histogram WBC Histogram

Red Blood Cell Histogram RBC Histogram
Platelet Cell Histogram PLT Histogram
2.2.6 Performance Specifications

2.2.6.1 Background or Blank Counting
Background counting is performed by the analyzer automatically when it is started up. The
following requirements must be met.
Blanking counting: test the diluent for 3 consecutive times, and take the highest result. The
following requirements must be met.
Table 2-5 Requirements on background or blank counting
Parameter Requirement
0.3 10 / L
9
WBC
12
RBC 0.03 10 / L
HGB 1g/L
HCT 0.5 %
9
PLT 5 10 / L
2-3
Analyzer Structure
2.2.6.2 Reproducibility
Run the sample meeting the reproducibility requirements for 10 consecutive times on the
analyzer, then calculate the CV(%) and SD, the requirements in Table 2-6 and 2-7 must be
met.
Table 2-6 Whole blood reproducibility requirements
Parameter Condition CV%/SD

2.5
7.0 ~ 15.0 10 / L
9
WBC
4.0 ~ 6.9 10 / L
9
3.5
3.50 ~ 6.50 10
12
/L 2.0
RBC
100 ~ 180 g/L 1.5
HGB
70.0~ 110.0 fL 0.5
MCV
4.0
150 ~ 500 10 / L
9
PLT 9
5.0
100~149 10 / L
3050% 3.0
HCT
Lymph% 2050 1.5SD
Mid% 213 1.5SD
Gran% 3070 3.0SD
Table 2-7 Predilute reproducibility requirements

Parameter Condition CV%
4.0 ~ 15.0 10 / L
9
WBC 4.0
12
RBC 3.50 ~ 6.50 10 /L 2.0
HGB 110 ~ 180 g/L 2.0
MCV 80.0 ~ 110.0 fL 1.5
9
PLT 100~500 10 / L 8.0
2.2.6.3 Linearity Requirements
Prepare and analyze samples of different concentration, calculate the slope and intercept by
linearity regression equation, then calculate the theoretical value, and the deviation between
the theoretical value and the test value, the requirements in Table 2-8 must be met.
Table 2-8 Linearity Requirements
Parameter Linearity range Deviation range (Whole Deviation range (Predilute

blood mode) mode)
9 9 9
WBC 0.0100.010 /L 0.3010 /L 5 0.5010 /L or 5
9
100.130010 /L 9 18
2-4
Analyzer Structure
12 12 12
RBC 08.0010 /L 0.0510 /L or 5 0.0510 /L or 5
HGB 0280g/L 2g/L or 2 2g/L or 3
9 9 9
PLT 0100010 /L 1010 /L or 10 1010 /L or 10
9
1001300010 /L 12 20
4% (HCT value) or 6% /
HCT 060%
(deviation)
Test suggestion:
12
When testing RBC linearity range 7.008.0010 /L, use sample of which the HCT 60%;
9
When testing PLT linearity range 1001300010 /L, use sample of which the RBC 6.
2.2.6.4 Carryover
The carryover impact of high concentration sample to low concentration sample.
Test method: mix the high value sample (centrifuged high value control or specialized high
value linearity control) within the range of Table 2-9, test the sample for 3 consecutive times,
and then mix and test the low value sample (diluted low value control, dilution rate 1:10) within
the range of Table 2-9 for 3 consecutive times, then calculate the carryover. The requirements
in Table 2-10 must be met.
Table 2-9 Requirements on high and low value controls of carryover calculation
Parameter Unit High concentration Low concentration

9
WBC 10 /L > 15.0 < 3.0
12
RBC 10 /L > 6.0 < 2.00
HGB g/L > 200 < 40
9
PLT 10 /L > 300 < 100
Table 2-10 Carryover requirements
Parameter Carryover
WBC 0.5
RBC 0.5
HGB 0.5
PLT 1.0
2.2.7 Functions
2.2.7.1 Startup and Shutdown
When starting up the analyzer, initialization will be performed to prepare the analyzer for
counting. Different startup cleaning sequence will be performed base on the latest shutdown
status to ensure the success of background counting. The analyzer provides the function to
skip fluidics initialization to the users of service level and above.
2-5
Analyzer Structure
The shutdown procedure will be performed according to different shutdown status.

2.2.7.2 Password and User Management
In order to protect the security of product setup and data, you have to possess the password to
be able to adjust the parameters and perform the extra functions.
2.2.7.3 Screen Navigation
The analyzer provides screen navigation function so that users can easily go to their target
screen.
2.2.7.4 Status Indication
The analyzer/system status indication function is provided on the analyzer screen or by
configuring LED in the hardware system.
2.2.7.5 Setup
Users can customize the settings of the analyzer on the software screen.
1. The configuration files are saved in the analyzer.
2. The analyzer must allow users to modify certain configurations. E.g.: Users can set
up date/time, reference range, parameter unit, communication-related parameters,
print format, etc.
3. For the FDA model, users can set whether to display or print RUO parameters
through the authorization protocol.
4. For the FDA model, users can set "QC lockout", if this function is activated, sample
analysis can be started only after the daily QC passes.
2.2.7.6 Standby
The analyzer will enter standby mode when no fluidic operation has been performed for a
certain period of time. The analyzer will exit the standby mode when operation is triggered
by users.
2.2.7.7 Self Test
The analyzer system provides self test and inspection function to ensure proper running.
2.2.7.8 Sample Review
After analyzing a sample, the analyzer saves the sample information and analysis result
automatically. Operators can review all parameters and histograms of the saved samples.
2.2.8 Sample Compartment

The tube holder in sample compartment can be fitted with adapters of different specifications.
The specifications of supported tubes are:
Evacuated collection tube: diameter: 1013.5mm; height (with cap): 4085mm.
Capillary collection tube: diameter: 1013.5mm; height (with cap): 4065mm.
Centrifugal tubes.
2-6
Analyzer Structure
2.2.9 Operating, Storing and Running Environment
Table 2-11 Temperature and Humidity Requirements
Optimal operating Storage environment

Running environment
environment
Temperature 1530 -1040 1535
Humidity 30%85% 10%93% 30%85%
Atmospheric 70kPa106kPa 50kPa106kPa
70kPa106kPa
pressure
2.3 System Composition

2.3.1 Functional Composition
The analyzer system has the following functions: reagent system, sample distribution, sample
preparation, sample analysis, signal processing, parameter analysis, data management, status
monitoring, schedule control, information processing, human-machine interface, power source,
cleaning and maintenance. The relations of the functional modules are shown in Figure 2-2.
Under the control of the schedule control and information processing functional module, the
other modules work together according to the designed procedure and requirement to realize
the core task of the analyzer - sample analysis.
Fluid Air flow Reagents Signal stream
2-7
Analyzer Structure
Figure 2-2 System function block diagram
2.3.2 Reagents
In order to ensure the accuracy of analysis results, you shall use the reagents, controls
and calibrators specified by Shenzhen Mindray Bio-medical Electronics Co., Ltd. For
details, refer to Table 2-12.
Table 2-12 Applicable reagents, controls calibrators
Item Name
Diluent M-30D DILUENT

Lyse M-30CFL LYSE
Rinse M-30R RINSE
Probe cleanser PROBE CLEANSER
Control B30 or BC-3D
Calibrator S30 or SC-CAL PLUS
2.4 Software Structure

2.4.1 Menu Structure
The top level structure of the system menu includes 8 functional options: analysis, review, QC,
calibration, setup, service, logoff and shutdown. Operators can enter the screens to perform
the functions of the analyzer through the system menu. The " " indicates there is sub-menu
under the option. Refer to Figure 2-3 -- Figure 2-7 for the full menu structure.
The menu structure of service access level:
2-8
Analyzer Structure
Figure 2-3 Top level menu and QC sub-menu
Figure 2-4 Top level menu and calibration sub-menu
2-9
Analyzer Structure
Figure 2-5 Top level menu and setup sub-menu
Figure 2-6 Top level menu and setup sub-menu(2)
2-10
Analyzer Structure
Figure 2-7 Top level menu and service sub-menu
2.4.2 Password
The login service ID is "Service", password is "s 3600!", case sensitive and space required.
With service access level, you can perform all functions that are open to common users and
some other special functions:
1 Transfer factor calibration function:
1 Under whole blood mode, click "Menu">"Calibration">"Calibrator", finish the
calibration successfully, exit and save the calibration factors.
2 Click "Menu">"Calibration">"Transfer factor" to enter the screen in Figure 2-8,
run the sample used in the above step for 5 consecutive times under whole blood
mode, check if CV(%) meets the requirements in Table 2-13; if not, cancel a abnormal
result and calculate again (results of at least 5 analyses required), then check if the
requirements in Table 2-13 are met.
2-11
Analyzer Structure
Figure 2-8Analysis screen of transfer factor under whole blood mode
Table 2-13 Whole blood CV range

Param WBC RBC HGB MCV PLT
eter
CV 2.0% 1.5% 1.5% 0.5% 4.0%
value
3 Click the "Predilute" button to switch to the screen of predilute mode, as Figure 2-9
shows;
2-12
Analyzer Structure
Figure 2-9 Analysis screen of transfer factor under predilute mode
4 Click the Diluent icon, perform diluent dispensing for 7 consecutive times;
5 Add 140l of calibrator in to the diluent, mix thoroughly and then run the mixture for
more than 5 times under the predilute mode. The CV(%) values must meet the
requirements of Table 2-14, and the calibration factors are within the range [0.75,
1.25].
Table 2-14 Range of CV value under predilute mode
Param WBC RBC HGB MCV PLT
eter
CV 4.0% 2.0% 2.0% 1.5% 8.0%
value
2 The gain of WBC and RBC can be adjusted, see Figure 2-10;
2-13
Analyzer Structure
Figure 2-10 Gain setup screen
3 In the service setup screen, you can set up languages, software upgrade, volume of the
volumetric tube, analyzer SN, sample probe positioning adjustment, background
information monitoring (auto adjust of analysis time), rinse photocoupler calibration, etc.
The following section will introduce the sample probe positioning adjustment and rinse
photocoupler calibration functions.
1) Sample probe positioning adjustment:
Tool: the positioning calibration clamp of sample probe sampling position
Procedure: open the cover as Figure 2-11 shows, open the sample compartment door,
take out the tube holder adapter from the compartment, put the positioning calibration
clamp to the sample compartment, and then close the compartment door.
2-14
Analyzer Structure
Figure 2-11 Sample compartment

Click the "Aspirate pos." under the "X direction motor" as shown in Figure 2-12, and click
"Start", then click the "Move Left" or "Move Right" button to move the sample probe to the
top of the positioning hole of the fixture, check if the tip of the probe is right against the
positioning hole, then click "Save" to save the setting.
Figure 2-12 Sample probe positioning adjustment

Note: the tip of the probe must be right against the positioning hole (judge by visual
inspection).
2) Rinse photocoupler calibration
Tool: 101 slot-headed screwdriver
Procedure: as Figure 2-13 shows, click "Menu""Setup""Service
Setup""Photocoupler Cal.", the dialog box shown in Figure 2-14 will pop up.
2-15
Analyzer Structure
Figure2-13 Rinse photocoupler calibration menu
Figure2-14 Rinse photocoupler calibration screen
2-16
Analyzer Structure
Click "Start" and then click "OK", the dialog box "Priming diluent" will pop up, then an
instruction message will be displayed, use the slot-headed screwdriver to rotate the
potentiometer VR1(RINSE) on the drive board (see Figure 2-15) counter-clockwise
until the D22 (RINSE-LED) turns on. Note: continue to rotate the VR1 for 180 degrees
after the D22 turns on.
Figure 2-15
Connect the connector of the rinse pickup tube to the fitting on the back panel of the
analyzer, click "OK" and then click "Finish", the dialog box "Priming rinse" will pop up,
check the calibration status then: if "Pass" is displayed (see Figure 2-16), that means
the calibration succeeded. The reference voltage and measured voltage are displayed
as "Reference(V)" (1.8-3.0V) and Actual(V) in Figure 2-16.
"PASS"
displayed
Figure 2-16
Note:
Rotate the VR1 for 180 degrees more after the D22 turns on, and make sure the LED
is illuminated.
Do not modify the value in the edit box Max(V), Min(V) and Factor(V);
If calibration cannot be done successfully due to the quality of the photocoupler itself
and the PCBA or photocoupler must be replaced, then recalibration is needed. The
dialog box "Remove the Rinse tube from the container and press [OK] to continue."
will pop up, press [OK] to drain the tubing; then do as instructed by the prompt - "1.
Adjust rinse photocoupler potentiometer till the RINSE-LED turns on; 2 Put the Rinse
tube into the container and press [OK]to close the dialog box; 3. Click [Finish] button"
to prime rinse after calibration is done.
2-17
Analyzer Structure
4 The reproducibility and carryover functions are available.
Figure 2-17 Menu of reproducibility and carryover

5 Check the replacement status of boards and CF card automatically. If the software
system is started and logged in by user of service level, then a prompt will guide the user
to restore the critical data.
Figure 2-18
1) If a new CF card is replaced, follow the guidance to select "Restore" and restore the
critical data to the CF card.
2) If a new data board is replaced, follow the guidance to select "Backup" and backup the
data to the data board.
3) If only the drive board is replaced, no special action shall be taken, the data will not be
2-18
Analyzer Structure
lost.
6 Users of the service level can export special information, see the figure below.
Figure 2-19
2.5 LIS Connection

2.5.1 LIS Setup and Adjustment
1. Click "Menu""Setup""System Setup""Communication", the following screen will
be displayed.
Figure 2-20 Communication setup screen
2-19
Analyzer Structure
2. Select network communication or serial port communication base on the physical

medium used.
3. If serial port communication is selected, communication can be realized when the
parameter settings of the serial port are identical with those of the LIS software. The
handshake function shall also be set up base on the LIS software used.
4. If network communication is selected, it is recommended that direct-coupled network
wire is used, and the major IP addresses such as 192.168.0. or 10.0.0.* are set up.
When connected to LAN, set up the IP address per the IP rules of the LAN. ACK
synchronous transmission shall also be set up base on the LIS software settings. If
you are not sure about that, use the default setup.
2.5.2 Connection Testing

It is recommended that you use Mindray data management software to perform actual
communication testing, or you can use the testing software developed by a compatible
software developer.
2.5.3 Analysis of Communication Failure

1. For abnormal serial port connection, check parameter settings. If the settings are
identical to those of the analyzer, replace serial port wire.
2. For abnormal network connection, check parameter settings. If the settings are correct,
check if the connection is normal by using ping. (If network firewall is installed in the
computer of the LIS end, turn off the firewall). If the ping command fails to restore the
connection, check if the network wire is well connected.
If physical connection is good, and the communication parameters and protocol settings are
correct, but communication failure persists, check if the LIS software complies with the
communication protocol. Refer to the operator's manual for the communication protocols.
2-20
3 Upgrade
3.1 Software upgrade
3.1.1 Preparation
1The whole software upgrade procedure will take about 6~11mins.
2What to do if there is an ERROR during the upgrade

1If there is a Mounting Failureplease restart the analyzer and run software upgrade again.
2
If there is a Upgrade Failure, please check the upgrade package in your USB flash disk and
run software upgrade again. Do NOT restart or power off the analyzer immediately.
3Requirements of the USB flash disk used in this upgrade.

1Please use well-known brand USB flash disk.
2Format(FAT32) the USB flash disk before use it.
3Do NOT remove the USB flash disk during the upgrade.
3.1.2 Upgrade procedure

1Copy the update file to a formatted USB flash disk.
2Insert the USB flash disk into the USB interface of analyzer (See figure 3-1)
Figure 3-1
3Log in the BC-3600 with Service Engineer username and password.
4Run Setup Service Setup Setup Start Upgrade (see figure 3-2), and then click Yes
in the dialog box (see figure 3-3).
3-1
Upgrade
Figure 3-2
Figure 3-3
NOTES:
1Make sure the UBS storage connected well to the USB interface of the Analyzer.
2After you click Yes to start the upgrade, the system will start to read and analyze the
configuration in the UBS storage and this will last for about 1min. Please wait for the pop-up
upgrade confirmation dialog box.
3Do NOT remove the USB flash disk during the upgrade.
3-2
Upgrade
5software upgrade.
1) See the following software upgrade screen:
Figure 3-4
2) Click the Upgrade button to start the upgrade. You will see the upgrade preparation dialog.
(See figure 3-5)
Figure 3-5
3) The upgrade preparation lasts for about 1 min, after that, you will see the upgrade confirm
dialog. (See figure 3-6)
Figure 3-6
4) Click Yes to start the upgrade. (See figure 3-7)
3-3
Upgrade
Figure 3-7
5) You may need to restart the instrument for several times during the upgrade. (See figure
3-8)
Figure 3-8
6) Upgrade succeeded. (See figure 3-9)
Figure 3-9
7) Upgrade failed. (See figure 3-10)
3-4
Upgrade
Figure 3-10
8) After you finish all the process, please restart the instrument. (See figure 3-11)
Figure 3-11
6After upgrade finished, log in with the service engineer username and password. Run
Service Version to check upgrade items. (See figure 3-12)
Figure 3-12
3-5
Upgrade
3.1.3 Notes and Prompts of Errors

1During the upgrade, the software will check whether the hardware and software are
matched. If not, select the proper language, click OK to start upgrade. (See figure 3-13)
Figure 3-13
2If upgrade failed, export the debug data. (See figure 3-14)
Figure 3-14
3-6
4 Mechanical System
4.1 Front of the Analyzer
Figure 4-1 Front of the analyzer(closed tube model)
1 --- Touchscreen 2 --- Indicator

3 --- Sample compartment door 4 --- USB interface
5 --- Recorder
4-1
Mechanical System
Figure 4-2 Front of the analyzer(open vial model)
1 --- Touchscreen 2 --- Indicator

3 --- Sample probe 4 --- Aspirate key
5 --- USB interface 6 --- Recorder
4-2
Mechanical System
4.2 Back of the Analyzer
Figure 4-3 Back of the analyzer
1 --- USB interface 1 2 --- USB interface 2

3 --- Network interface 4 --- Serial port
5 --- Power switch 6 --- Waste outlet
7 --- Rinse outlet 8 --- Diluent outlet
4-3
Mechanical System
4.3 Left of the Analyzer
Figure 4-4 Left of the analyzer (open vial model)
1 --- Top cover 2 --- Front cover assembly

3 --- Side door of electrical system 4 --- Back panel assembly
5 --- Bottom panel assembly
4-4
Mechanical System
Figure 4-5 Left of the analyzer (closed tube model)

3 --- Side door of electrical system 4 --- Back panel assembly
4-5
Mechanical System
4.4 Right of the Analyzer
Figure 4-6 Right of the analyzer (open vial model)

3 --- Side door of fluidic system 4 --- Door lock
5 --- Aspirate key 6 --- Back panel assembly
4-6
Mechanical System
Figure 4-7 Right of the analyzer (closed tube model)

3 --- Side door of fluidic system 4 --- Door lock
5 --- Aspirate key 6 --- Back panel assembly
4-7
5 Fluidic System
5.1 Fluidic Components and Functions
5.1.1 Sample Probe

Aspirating and dispensing sample.
5.1.2 Probe Wipe

Cleaning the exterior and interior walls of the sample probe.
5.1.3 Pump
Gas pump (GP): provide pressure to the vacuum chamber for generation of bubbles.
Waste pump (LP): draining probe wipe, WBC bath, RBC bath and vacuum chamber
and building vacuum.
5.1.4 Syringes
Sampling syringe (Asp-Syringe): full measuring range is 100l; aspirating fixed
quantity of sample, dispensing the sample and aspirating the diluted sample again.
Diluent syringe (Dil-Syringe): full measuring range is 10ml; dispensing fixed quantity
of diluent to WBC and RBC bath, providing diluent to probe wipe.
Lyse syringe (Lyse-Syringe): full measuring range is 2.5ml, motivated by the same
motor with the diluent syringe; dispensing lyse.
5.1.5 Valves
Fluid valve and magnetic valve: diaphragm form, controlled by electric magnetic
power. Controlling the directional flow of fluid or air.
5.1.6 Baths
WBC bath: composed of front bath, back bath and aperture. Providing room for the
mixing of WBC sample; measuring HGB and WBC.
RBC bath: composed of front bath, back bath and aperture. Providing room for the
mixing of RBC sample; measuring RBC/PLT.
Vacuum chamber: build and store stable vacuum for the impedance counting of WBC
and RBC, clean the back baths and drain volumetric tubes.
Pressure chamber: build and store stable pressure for generation of bubbles in all
baths.
5-1
Fluidic System
WBC isolation chamber: providing air lock to block outside disturbance.
RBC isolation chamber: providing air lock to block outside disturbance.
5.1.7 Volumetric Tubes

WBC volumetric tube: its volume is 300ul, measuring volume of the WBC sample to
be analyzed.
RBC volumetric tube: its volume is 200ul, measuring volume of the RBC sample to
be analyzed.
5.1.8 Filters
Filter of WBC volumetric tube: filtrate the air entering the WBC volumetric tube.
Filter of RBC volumetric tube: filtrate the air entering the RBC volumetric tube.
GP filter: filtrate the air entering the gas pump.
Customized filter: filtrate the impurities and scraps from the probe wipe.
Filter of the isolation chamber: filtrate the impurities and scraps from the baths.
5.2 Reagent Consumption
There are four counting modes: OV-WB, OV-PD, CT-WB and CT-PD. See Table 5- 1 for the
reagent volume consumed in one analysis cycle of a sample.
Table 5- 1Reagent consumed in one analysis cycle of a sample
Reagent consumed in
one analysis cycle of a
Item Name
sample
Whole blood Predilute
Diluent M-30D DILUENT 28mL 28mL
Lyse M-30CFL LYSE 0.35mL 0.35mL

Rinse M-30R RINSE 8mL 8mL
Probe 0 0
PROBE CLEANSER
cleanser
Control B30BC-3D /
Calibrator S30SC-CAL PLUS /
5-2
Fluidic System
5.3 Sample Dilution Flowchart
5.3.1 Whole Blood Mode

The whole blood analysis cycle completes in 60s. The analysis procedure is as follows:
1) Aspirate 17ul of sample, the sample probe ascends and the exterior wall of the probe is
cleaned;
2) Dispense 6ul of sample to the probe wipe to wash it off;
3) Add diluent into the WBC bath and dispense 9ul of sample into the WBC bath, mix them to
get the first dilution sample for WBC and HGB measurement.
4) The sample probe ascends and its exterior wall is cleaned, the residual sample is washed
off by diluent in the probe wipe.
5) The sample probe descends to the WBC bath and aspirate 34ul of sample again, diluent is
added and the second dilution sample is dispensed into the RBC bath, and mix them for
RBC/PLT counting.
6) Add lyse into the WBC bath, and mix thoroughly;
7) Analyzing the sample;
8) When the analysis completes, clean and zap the WBC/RBC bath, release the pressure in
the vacuum chamber.
See Figure 5-1 for the dilution procedure of whole blood mode:
Figure 5-1 Dilution procedure of whole blood mode
5-3
Fluidic System
5.3.2 Predilute Mode

The predilute analysis cycle includes 3 function sequences (2 for closed tube models): diluent
dispensing sequence, cleaning sequence (open vial models) and analyzing sequence. The
procedure is as follows:
1) The sample probe dispense 380ul of diluent to a centrifugal tube and 20ul of sample in the
capillary tube is added in to the centrifugal tube, mix them to form the diluted sample;
2) The sample probe aspirate 146.34ul of sample, and diluent is added into the WBC bath to
form the first dilution sample for WBC and HGB measurement;
3) The sample probe ascends and its exterior and interior walls are cleaned;
4) The sample probe descends to the WBC bath and aspirate 37.39ul of sample again, diluent
is added and the second dilution sample is dispensed into the RBC bath, and mix them for
RBC/PLT counting.
5) Add lyse into the WBC bath, and mix thoroughly;
6) Analyzing the sample;
7) When the analysis completes, clean and zap the WBC/RBC bath, release the pressure in
the vacuum chamber.
You can see from the above procedures that the analysis cycles of predilute mode and whole
blood mode are only different in the operations of sampling and dispensing into WBC bath,
therefore this section will only introduce the different parts.
See Figure 5-2 for the dilution procedure of predilute mode:
5-4
Fluidic System
Figure 5-2 Dilution procedure of predilute mode
5.4 Introduction of Fluidic Channels
5.4.1 WBC/HGB Channel

All reagents:1)M-30PCFL lyse (lysing HGB, PLT and separating basophils from
other WBCs ); 2) M-30PD diluent (cleaning, providing reaction and measurement
environment)
Analysis principles: impedance method (analyzing WBC); colorimetric method
(analyzing HGB)
Analysis parameters: WBC, HGB
Graphic information: WBC histogram
Dilution ratio:1:302 (WB); 1:369.77 (PD)
Measurement volume: 300l
Function description: Mix 9ul of blood and 2.35ml of diluent in the WBC bath,
provide 34ul of first dilution sample to the RBC bath and add 0.35ml of lyse into it,
mix them; then aspirate the mixed sample into the back bath through the aperture by
the vacuum of the vacuum chamber. The cells are analyzed when they pass the
aperture. Volume of the sample analyzed is measured by the volumetric tube.
5-5
Fluidic System
5.4.2 RBC/PLT Channel

All reagents: diluent (diluting, cleaning, providing conductive environment, isometric
processing of cells)
Analysis principles: impedance method
Analysis parameters: RBC, PLT
Graphic information: RBC histogram, PLT histogram
Dilution ratio:1:20000 (WB); 1:22333.07 (PD)
Measurement volume: 200l
Function description: the sample probe aspirates 34ul of sample (dilution ratio:
1:262.11) from the WBC bath, add 0.8ml of mixture of the sample and diluent into the
RBC bath to form the sample of dilution ratio 1:20000 with another 1.8ml of diluent;
Mix them and aspirate the sample by vacuum of the vacuum chamber into the back
bath through the aperture. The cells are analyzed when they pass the aperture.
Volume of the sample analyzed is measured by the volumetric tube.
5.5 Introduction of Basic Sequence
5.1.1 Whole Blood Analysis Sequence

This section introduces the working procedure of sample probe in the measurement sequence
first, then all the other working procedures are introduced in detail.
5.5.1.1 Sampling and dispensing procedure
The working procedure of sample probe is indicated in Figure 5- 3. See section 5.3 for the
sample dilution procedure. The working procedure of sample probe:
a) Sample aspirating
piercing(closed tube models)
aspirating 17ul of sample.
b) First dilution
When aspiration finishes, the sample probe ascends;
The sample probe moves to the top of the WBC bath;
The sample probe descends to the WBC bath and dispenses 9ul of sample.
c) Aspirating sample from the WBC bath
The sample probe ascends from the WBC bath, and removes the residual
sample in the probe wipe;
The sample probe descends to the WBC bath and aspirates 34ul of first dilution
sample.
d) Second dilution in the RBC bath
When aspiration finishes, the sample probe ascends from the WBC bath;
The sample probe moves to the top of the RBC bath;
The sample probe descends to the RBC bath, and dispenses 0.8ml of mixture of
the sample and diluent.
5-6
Fluidic System
Initialize position of the sample probe by command INIT.
? ?
RBC WBC
Blood
Diluent
sample
a) b)
?
? ? ?
?
RBC WBC RBC WBC
First Secon
dilutio d
n dilution
c) sampl sample
d)
e
Figure 5- 3 Sampling and dispensing procedure
5.5.1.2 The measurement procedure

This section highlights the whole blood measurement sequence of the closed tube models.
The measurement sequence consists of sample aspiration, dispensing, dilution, mixing,
counting, and cleaning.
Sampling (0~12.6s)
The actions are:
Pre-piercing: the pre-piercing process of sample probe (for closed tube models)
to release the pressure in the tube before sample aspiration.
Cleaning the exterior and interior walls of sample probe: when piercing finishes, the sample
probe ascends to home position, meanwhile its exterior and interior walls are washed. See the
following figure.
5-7
Fluidic System
V01 V01
Diluent Diluent
V02 V04 V02 V04
10ml 10ml
100ul 100ul
V19 V19
Waste Waste
a) Wash exterior wall of b) Wash interior wall of
sample probe sample probe
Figure 5- 4 Fluidic passages of sample probe cleaning

The sample probe descends to the sampling position, and then moves to the
piercing position, the syringe withdraws for 2ul to create separating bubbles.
Then the sample probe descends to the lowest aspirating position to prepare for
sample aspirating. Besides, the fluidic passages of draining and dispensing of
the WBC bath are indicated in Figure 5- 5 and Figure 5- 6; Under the effect of
vacuum, rinse flows through and washes the WBC and RBC back baths and
their volumetric tubes, the fluidic passage of washing the WBC back bath and
volumetric tube is indicated in Figure 5- 7; When the washing progress ends,
Valve 5 and 6 opens to drain the volumetric tubes.
5-8
Fluidic System
V01
Diluent
V04
V02 V03
10mL
2.5mL
(SR1) SR3
WBC
bath Lyse
Isolation
chamber
V16
Waste
Figure 5- 5 Fluidic passage of draining of the WBC bath
V01
Diluent
V04
V02 V03
10mL
2.5mL
(SR1) SR3
WBC
bath lyse
Figure 5- 6 Fluidic passage of dispensing of the WBC bath
5-9
Fluidic System
V05 V07
Atmosp WBC
heric bath
pressure
Volumetric
board
Pressure V14
Rinse
Vacuum
chamber
V09
Isolation
V10 chamber
V18
V16
Waste
Figure 5- 7Fluidic passages of washing WBC back bath and volumetric tube
Aspirating sample: the sampling syringe aspirates 17ul of sample at 6.5~8.5s;

and 800ul of diluent is added to the RBC bath after it is drained;
Ascending to home position: the sample probe ascends to home position at
9.3~11.6s, and its exterior wall is washed; by now, the sampling procedure is
finished.
Other actions finished during the sampling procedure:
After sample dispensing of the WBC bath finishes (7s) and the fluid level
stabilizes, the HGB voltage and WBC aperture voltage (the constant-current
source is opened at 10.5s in advance) is read at 10.5s.
Sample Dispensing, Dilution and Mixing (11.5~30.8s)
The actions are:
Dispensing sample for the first time: the sample probe removes 6ul of sample in
the probe wipe at 11.5~12.3s.
Dispensing sample for the second time: the sample probe moves to the top of
WBC bath and descends into the bath; the diluent syringe dispenses 1150 ul of
diluent into the WBC bath, then the sample probe dispensed 9 ul of sample and
swings; when sample dispensing finishes, the diluent syringe dispenses 1200ul
of diluent into the WBC bath again; Valve 10 opens, and blows one small bubble
and 3 big bubbles into the WBC bath in turn. By now, the first dilution of sample
finishes.
The sample probe ascends with its exterior and interior walls washed: when the
second sample dispensing finishes, the sample probe ascends to home position
with its exterior and interior walls washed; besides, 2700ul of diluent is
dispensed into the RBC bath.
5-10
Fluidic System
Second aspiration in the WBC bath: the sample probe descends to the WBC
bath and aspirates 34 ul of fluid at 19.9~21s.Valve 17 opens, and the RBC bath
is drained.
The sample probe ascends: when the second aspiration finishes, the sample
probe ascends to home position with its exterior wall washed; besides, 1800 ul
of diluent is added to the RBC bath at 24.5~25.5s.
Adding sample to RBC bath, and lyse to WBC bath: the sample probe moves to
the RBC bath and descends into the bath; the lyse syringe dispenses 350ul of
lyse into the WBC bath, the fluidic passage is indicated in Figure 5- 8; the diluent
syringe discharges 800 ul of fluid (mixture of first dilution sample and diluent)
through the sample probe. Valve 10 opens at 26.65~27.25s, the pressure
chamber blows bubbles into the WBC bath; then a bubble that can last for 0.05s
is blowed into the WBC bath, and 3 bubbles are blowed into the RBC bath in
turn; Finally, the sample probe restores to the original position with its exterior
wall washed.
V03
2.5mL
(SR1)
WBC
bath
Lyse
Figure 5- 8 Fluidic passage of lyse dispensing of the WBC bath
Other actions finished during the sample dispensing, dilution and mixing
procedure:
Build the vacuum needed for analysis, total time consumed is 7.9s;
Build the pressure needed for bubble generation;
RBC aperture voltage inspection: same as WBC aperture voltage inspection;
The sample compartment door opens at 12s to prepare for the next analysis
cycle.
Analysis
The actions are:
Measuring volume of volumetric tube: valve 7 opens after WBC bubbling
finishes for a while and valve 8 opens after RBC bubbling finishes for a while,
the fluidic passage is indicated in Figure 5- 9; the closing of valve 7 and valve 8
is finished by the ending cleaning cycle started when the WBC and RBC down
photocouplers are triggered.
5-11
Fluidic System
V05 V07
V06 V08 RBC WBC

bath bath
Volumetric Volumetric
board board
V14
Vacuum V15
chamber Rinse
V18
Waste
Figure 5- 9 Fluidic passages of WBC and RBC analysis
The photocoupler of volumetric board is opened at 25.7s.

Cleaning when analysis ends
The actions are:
The ending cleaning sequence is an independent sequence, it is started by the
software automatically base on the information obtained during the analysis;
Fluid discharging of RBC and WBC baths: open valve 17, the RBC bath starts to
discharge fluid; open valve 16, the WBC bath starts to discharge fluid;
Fluid dispensing of RBC and WBC baths: 1400 ul of fluid is dispensed into the
RBC and WBC bath respectively;
Washing RBC and WBC back baths: when valve 8 and valve 15 open at the
same time, the RBC back bath will be washed by rinse under the effect of
vacuum; when valve 7 and valve 14 open at the same time, the WBC back bath
will be washed by rinse under the effect of vacuum.
Draining the vacuum chamber: open valve 9 to drain the vacuum chamber.
Resetting of the diluent syringe and sample probe assembly: the diluent syringe
aspirates 2100 ul of fluid, then valve 1 opens, the syringe returns to home
position and dispenses fluid to the RBC bath at the same time; the sample probe
assembly returns to home position.
The sample probe aspirates air bar and returns to home position: the sample
probe moves to the piercing position and withdraws for 2ul and then returns to
home position.
Zapping: zapping starts when fluid dispensing of WBC bath completes for a
while.
Calculating the volume of rinse consumed: the software reads from the
command to calculate the volume of rinse consumed.
Detecting rinse status: sensor in the analyzer detects if the rinse container is
empty; when alarming for no rinse, the residual volume of rinse must be able to
support the on-going analysis.
The major difference between the whole blood analysis sequences of open vial models and
closed tube models lies in the sampling procedure, the open vial sequence involves no
piercing action, but its sampling time point and the cleaning action after sampling are the same
5-12
Fluidic System
with those of the closed tube sequence, therefore the whole blood analysis sequences of open
vial models will not introduced in detail.
5.1.2 Predilute Measurement Sequence

The predilute analysis cycle includes 3 function sequences (2 for closed tube models): diluent
dispensing sequence, cleaning sequence (open vial models) and analyzing sequence.
The predilute measurement procedure: (1)The sample probe dispense 380ul of diluent to a
centrifugal tube and 20ul of sample in the capillary tube is added in to the centrifugal tube, mix
them to form the diluted sample. (2)The sample probe aspirate 146.34ul of sample, and diluent
is added into the WBC bath to form the first dilution sample for WBC and HGB measurement.
(3)The sample probe ascends and its exterior and interior walls are cleaned. (4)The sample
probe descends to the WBC bath and aspirate 37.39ul of sample again, diluent is added and
the second dilution sample is dispensed into the RBC bath, and mix them for RBC/PLT
counting. (5)Add lyse into the WBC bath, and mix thoroughly; (6)Analysis. (7)When the
analysis completes, clean and zap the WBC/RBC bath, release the pressure in the vacuum
chamber.
You can see from the above procedures that the analysis cycles of predilute mode and whole
blood mode are only different in the operations of sampling and dispensing into WBC bath,
therefore this section will only introduce the different parts.
See section 5.3.2 for the dilution procedure of predilute mode.
5.5.2.1 Dilution dispensing sequence
The procedure is indicated in Figure 5- 10:
a) closed tube models b) open vial models
Figure 5- 10 Diluent dispensing flowchart
5.5.2.2 Cleaning sequence after diluent dispensing

Introduction:
5-13
Fluidic System
1. This sequence applies to open vial models only.

2. The sequence cleans the exterior wall of the sample probe, initializes diluent syringe
by command INIT; the sampling syringe withdraws for 2ul to create a separating air
bar, the system restores to "Ready" status.
5.5.2.3 Predilute Measurement Sequence
Introduction:
Sampling:
1. The diluent syringe withdraws for 100ul through the probe wipe tube;
2. WBC/RBC bath is zapped for 3s;
3. The diluent syringe aspirates 146.34ul of sample;
4. When aspirating finishes, valve 01 closes 0.1s after valve 04 closes.
Sample dispensing of WBC bath:
1. The diluent syringe pushes sample in the sampling tube into the WBC bath to form
the first dilution sample;
2. Sample volume of the second aspiration is 37.39ul;
3. The predilute measurement takes 1.5s more than the whole blood measurement due
to the zapping action added before analysis;
4. The other parts of sequence are the same as those of the whole blood measurement
sequence, only the fluid volumes are different in some steps.
5. The major difference between the closed tube and open vial models is the position of
the sample probe under the "Ready" status.
5.1.3 Startup/Shutdown Sequence

5.5.3.1 Startup sequence
There are three types of startup sequence: 1 startup after normal shutdown; 2 startup after
abnormal shutdown; 3 startup after performing "Prepare To Ship" procedure.
Startup after normal shutdown
This sequence includes the troubleshooting sequence 1, fluidics initializing sequence and
startup sequence after normal shutdown.
1. Troubleshooting sequence 1, refer to the corresponding section.
2. Fluidics initializing sequence, refer to the corresponding section.
3. Startup after normal shutdown
5-14
Fluidic System
Figure 5- 11Startup procedure after normal shutdown

Introduction:
1 There are zapping and flushing operations at the beginning to avoid clogging after
long term of probe cleanser soak.
2 0.5ml of lyse is discharged to make sure the tube mouth is filled with reagent.
3 Push back 200ul of diluent to the cleanser tube to wash off the cleanser at the
closed end of valve 12.
4 Empty RBC charging line to avoid crystallizing of the cleanser spills on the wall of
the bath which may influence the PLT background.
5 Empty the sample probe and dispense fluid: after emptying the sampling tube,
5-15
Fluidic System
dispense fluid at a higher speed so that the bubbles in the sampling tube can be
removed effectively.
6 The sample probe dispenses fluid in the WBC bath: removing all the bubbles in
the sampling tube.
7 Cleaning the two baths: cleanser volumes of WBC bath 4ml, 4.6ml, 5.5ml;
cleanser volumes of RBC bath 4ml, 4ml, 5ml, generating bubbles during the
cleaning process to strengthen the cleaning effect.
8 Emptying WBC, RBC baths and the back baths, switching among valve 16 ,17, 7
and 8 to strengthen the cleaning effect.
Startup after abnormal shutdown
startup sequence after normal shutdown.
3. Startup after normal shutdown
Figure 5- 12 Startup procedure after abnormal shutdown

Introduction:
1 When starting up the analyzer after abnormal shutdown, there may be some
5-16
Fluidic System
residual sample in the fluidic components, so the exterior and interior walls of
the sample probe must be cleaned repeatedly by fluid discharging of the
sample probe and probe wipe.
2 0.5ml of lyse is discharged to make sure the tube mouth is filled with reagent.
3 Push back 200ul of diluent to the cleanser tube to wash off the cleanser at the
closed end of valve 12.
4 Remove the bubbles in the sampling tube through fluid discharging for multiple
times.
5 Clean the two baths repeatedly to remove the possible residual sample.
6 Zapping for 4s to avoid clogging.
7 Wash tubes of the back baths twice to remove the residual sample in the tubes
or volumetric tubes.
Startup after performing "Prepare To Ship" procedure

overall reagent priming sequence.
3. See section 5.5.3.5 for the overall reagent priming sequence.
5.5.3.2 Shutdown sequence

The probe cleanser soak sequence (general and intensified, refer to 5.5.4.7 and 5.5.4.8 for
details), intensified shutdown unclogging sequence (refer to 5.5.4.2 for details) and shutting
down sequence will be performed in turn during the shutdown process.
As per the different probe cleanser soak sequences, the shutdown sequence can be classified
into two kinds: 1 shutdown after general probe cleanser soak, 2 shutdown after intensified
probe cleanser soak.
The operations of the shutting down sequence are: washing the exterior wall of the sample
probe and soaking the probe in the WBC bath.
5.1.4 Cleaning/Maintenance Sequence

5.5.4.1 Unclogging sequence
Introduction:
1. Drain the two baths.
2. Valve 9 opens to connect the pressure chamber and vacuum chamber and build
pressure.
3. Flush the aperture for 4s and drain the fluid.
4. Add 3.5ml of fluid in the two baths respectively.
5. Zap the two baths for 4s respectively.
5-17
Fluidic System
6. Wash the tubes of the back baths. Empty the vacuum chamber and release vacuum.
5.5.4.2 Zapping sequence
Introduction:
1. Build vacuum in the vacuum chamber, zap the two baths for 4s.
2. Drain the two baths and add 3.5ml of fluid in the two baths respectively.
3. Wash the tubes of the back baths. Empty the vacuum chamber and release vacuum.
5.5.4.3 Flushing sequence
Introduction:
1 The flushing sequence is almost the same as the unclogging sequence, only it involves no
zapping operation.
5.5.4.4 Draining sequence of the baths
Introduction:
1 Drain the two baths for 3s.
5.5.4.5 Priming sequence of the baths
Introduction:
1. Drain the two baths and add 3.5ml of fluid in the two baths respectively.
2. The function is activated after the baths are drained. The baths may need to be
serviced during the draining process, the tubes of the back baths need to be washed.
3. Drain the vacuum chamber and release vacuum.
5.5.4.6 Cleaning sequence of the baths
Introduction:
1. 4.5ml of fluid is added to the two baths respectively, and bubbles are generated to
strengthen the cleaning effect.
2. Drain the two baths and add 3.5ml of fluid into the two baths respectively, open valve
18 and the fluidic pump for a while to release pressure in the pressure chamber.
5.5.4.7 General probe cleanser soak sequence
This sequence includes soaking sequence and the cleaning sequence after soaking.
Soaking procedure:
5-18
Fluidic System
Figure 5-13 Soaking procedure
Introduction: the soaking process consists of three steps. Step 1: soak the WBC aperture
and interior wall of the sample probe with 100% probe cleanser. Step 2: soak the WBC and
RBC front/back baths with 30.77% and 38.46% probe cleanser respectively. Step 3: soak the
WBC and RBC front/back baths with 26.32% and 21.05% probe cleanser respectively. The
operations are:
1. The sampling syringe withdraws for 2ul to form a separating air bar.
2. The sample probe aspirates 1.8ml of probe cleanser, the two baths are drained,
the sample probe ascends with its exterior wall washed.
3. The sample probe dispenses 1ml of probe cleanser into the WBC bath to form
100% probe cleanser.
4. The sample probe dispenses 2.6ml of mixture of probe cleanser and diluent into
the RBC bath to form the high concentration compound (30.77%) for the first
soak.
5. The sample probe dispenses 1.6ml of diluent into the WBC bath to form the high
concentration compound (38.46%) for the first soak.
5-19
Fluidic System
6. After soaking for about 2 minutes, dispenses 1.2ml of diluent to the two baths to
dilute the probe cleanser, which will be used for the long term soaking of the front
and back baths (WBC:26.32%; RBC:21.05%).
7. Build vacuum and aspirate fluid: aspirate for 6s from the WBC bath, the volume
aspirated is about 232ul; aspirate for 8s from the RBC bath, the volume aspirated
is about 236ul; When the aspiration finishes, release the vacuum in the vacuum
chamber.
8. Meanwhile, the sample probe aspirates 50ul of fluid from the RBC bath for
soaking of the interior wall of sample probe and the tube.
9. Soak the tubes for 2 minutes and then start the cleaning procedure.
Cleaning procedure:
5-20
Fluidic System
Figure 5-14Cleaning procedure
Introduction: the front and back baths, sampling tube, waste valve shall be cleaned to remove
residual probe cleanser.
1. Add probe cleanser to the WBC bath; when draining the WBC bath, switch on/off
valve 16 for multiple times to soak the waste valve with probe cleanser (for about
5s) to remove residual probe cleanser.
2. Add probe cleanser to the RBC bath; when draining the RBC bath, switch on/off
valve 17 for multiple times to soak the waste valve with probe cleanser (for about
5s) to remove residual probe cleanser.
5-21
Fluidic System
3. The sample probe dispenses 7.8ml of probe cleanser to the RBC bath to clean
the sampling tube and remove bubbles in the tubing.
4. Discharge fluid to the probe wipe twice, meanwhile the sample probe ascends to
avoid residual probe cleanser on the exterior wall of the sample probe or in the
probe wipe.
5. Build vacuum, wash tube of the back bath, switch on/off valve 7 and 8 for
multiple times.
6. Empty the vacuum chamber, switch on/off valve 18 for multiple times.
7. Drain the baths and build pressure to 40kpa.
8. Flushing the apertures.
9. Drain the two baths and add 3.5ml of fluid into the baths respectively, zap the
baths for 4s and build vacuum to -24kp.
10. Wash the back baths, switch on/off valve 7 and 8 for multiple times to strengthen
the cleaning effect of the valves and remove residual probe cleanser.
11. Empty the vacuum, switch on/off valve 18 for multiple times to strengthen the
cleaning effect of the valves and remove residual probe cleanser.
12. Release the pressure, the analyzer restores to "Ready" status.
5.5.4.8 Intensified probe cleanser soak sequence
Soaking procedure:
5-22
Fluidic System
Figure 5-15 Soaking procedure
Introduction: the intensified probe cleanser soak procedure is almost the same as the general
probe cleanser soak procedure, the only difference lies in the dilution ratio of probe cleanser.
5-23
Fluidic System
Cleaning procedure:
The cleaning operations of the intensified and general probe cleanser soak procedure
are almost the same, the only difference lies in the occurrence time of the operation.
5.5.4.9 Draining sequence of the tubing

Procedure:
Figure 5- 16Draining procedure of the tubing
Introduction:
1. Build vacuum in the vacuum chamber to drain the back bath and rinse
tubes.
2. Drain the RBC bath, the diluent syringe withdraws for 1.3ml to drain the
charging line of RBC bath.
3. The lyse syringe aspirates and discharges lyse to the WBC bath for
multiple times to drain the lyse tube.
5-24
Fluidic System
4. The diluent syringe aspirates and discharges diluent to the WBC bath for
multiple times to drain the diluent tube.
5. The sampling syringe aspirates 80ul of fluid.
6. The sample probe goes inside the WBC bath to drain the sampling tube.
7. Drain the charging line and the waste tube of the probe wipe.
8. Push the 80ul of fluid to the syringe inlet.
9. After build vacuum in the vacuum chamber, open the rinse tubes and empty
tubes of the back baths.
10. Empty the vacuum chamber and build vacuum at the same time, open V5
and V6 of the volumetric tube and dry the volumetric tube.
11. Release vacuum and pressure, the syringes return to home position, the
sample probe returns to the sampling position.
5-25
6 Hardware System
6.1 Introduction
The hardware system consists of 8 boards, which are power board, data board, drive board,
indicator board, touch screen control board, volumetric boars, sample compartment connector
board, and USB interface board; the system also includes the drive and detect components
that require power supply (such as motors, valves, pumps, sensors, display, and filter of input
power) and the wires connecting boards and components.
6.1.1 Function Diagram

See Figure 6-1 for the function diagram of the hardware system.
Figure 6-1Function diagram of the hardware system

The hardware system consists of five modules, which are system power, data stream channel,
central system, drive components and peripherals. The function of each module is as follows:
1. System power: providing power of various specifications to the boards, components
and devices.
2. Data stream channel: extracting, adjusting, amplifying and collecting and
preprocessing of signals.
3. Central control system: data collection and processing, result display, sample
storage, etc. Besides, the central control system controls and responds to all
peripherals.
4. Drive/detect components: controlling valves, pumps and motors, monitoring
photocouplers and other major parameters, collecting detection information and
6-1
Hardware System
sending alarms.
5. Peripherals and interfaces: display/touchscreen, recorder, USB interfaces (for printer,
keyboard and barcode scanner), Ethernet interface and LIS serial port. Besides,
peripherals includes status indicator, key-presses and aspirate key input.
6.1.2 Electric Connection Diagram

See Figure 6-2 for the electric connection diagram of the hardware system.
6-2
Hardware System
Figure 6-2 Electric connection diagram of the hardware system
6-3
Hardware System
6.1.3 Troubleshooting the System

The errors of the hardware system include board errors, wire errors and component errors.
Generally ,the troubleshooting methods of the three types of errors can be found in the following
sections; however, if the power supply of the hardware system cannot be ensured (the analyzer
cannot be electrified, or self-shields right after power on), the errors shall be fixed on the system
level.
1 Troubleshooting procedure when the analyzer cannot be electrified (the indicators of the drive
board and the data board are both off)
(1) Check if the AC input wire outside the analyzer is firmly connected, if not, re-plug the wire.
(2) Power off the analyzer, check if the wire of the power input filter is firmly connected, if not,
re-plug the wire.
(3) If step (1) and (2) cannot remove the error, replace the power input filter (see Figure 6-3);
if the error persists, do step (4).
Figure 6-3 Power input filter

(4) Replace the power board or assembly.
2 Troubleshooting procedure when the analyzer self-shields right after power on
(1) First, unplug the wires of the drive board and data board (see Figure 6-4), see if the power
can be started; if not, check if the wire is damaged; if so, replace the wire; otherwise replace
the power board or assembly. If the power can be started normally, the problem of power
assembly can be excluded.
Figure 6-4Sockets of drive board and power board
6-4
Hardware System
(2) After completing step (1), plug the two wires on of the drive board in turn to see if the power
can be started; if yes, the problem of the drive board can be excluded, the data board needs to be
replace; if no, unplug the wire of the volumetric board; if the power can be started, that means the
volumetric board or its wire is short-circuited, the volumetric board or its wire shall be replaced; if
not, that means the drive board is short-circuited, the drive board shall be replaced.
If the power can be started normally, troubleshoot the error per the following sections.
6.2 Data Board
6.2.1 Introduction
The data board consists of the analog and digital parts, the analog part filters, amplifies, outputs
and processes signals to obtain the signals that are suitable for A/D data conversion. The digital
part processes, outputs, controls and transmits data. It is the core part of the data board or even
the entire hardware system.
Section 6.2 is the servicing and troubleshooting guideline of the data board.
6.2.2 Structure
Introduction of Functions
See Figure 6-5 for the structural diagram of the data board.
Figure 6-5Structural diagram of the data board

You can see from Figure 6-5, the data board consists of the analog circuit and digital circuit. The
analog circuit adjusts and amplifies the WBC, RBC, PLT and HGB signals to make sure the
signals are turn and suitable for A/D conversion; the A/D module is the interface of the analog
circuit and digital circuit, it collects the sensing signals and other monitoring signals, and converts
analog signals into digital signals; the digital circuit processes data, stores and outputs results.
Besides, the digital circuit takes on the control and communication tasks, it is the core part of the
data board or even the entire hardware system.
1. Analog module
6-5
Hardware System
Figure 6-6 Structural diagram of the analog module

The analog module of the data board consists of RBC/PLT channel, WBC channel, HGB
channel and A/D conversion part, its functions are:
Adjusting analog power
Adjusting volume signal
Data collection
2. Digital module
Figure 6-7 Structural diagram of the digital module

The structure of the digital module is "CPUFPGA", see Figure 6-7.The functions of the
digital module are:
Preprocessing of data
Supporting peripherals interfaces
6-6
Hardware System
Extending control interfaces

Description
Adjusting analog power
Converting the input power into the power that meets the requirement of the board.
Adjusting volume signal
Driving, collecting, adjusting, amplifying the RBC, PLT, WBC and HGB signals, and sending
the signal to A/D part for conversion.
Data collection
The A/D circuit converts the analog signals in to digital signals that can be recognized by the
digital module (FPGA and CPU), and sends the signals to FPGA for processing.
Preprocessing of data
The FPGA filters the digital signals collected by the A/D part, saves the particle parameters
and sends the data to CPU through Flexbus by means like interrupt, etc.
Supporting peripherals interfaces
The CPU module provides the platform for software operation to the system, and provides
interfaces for peripherals, including interfaces of the drive board, LIS system, recorder, indicator
board, touchscreen control board, LCD display, Ethernet, USB printer, USB barcode scanner,
keyboard and USB, etc. It also provides JTAG interface for the online programming of FPGA
configuration chip and BDM interface for CPU debugging.
Extending control interfaces
The FPGA provides control logic and interfaces for LCD, recorder, CF card, touchscreen and
door detector.
Definition of Interfaces
There are 19 interface sockets on the data board, the functions of the interfaces are listed in
Table 6-1. See Figure 6-8 for the position of the interfaces.
Table 6-1 Functions of data board interfaces
Interface Function No. of pins Description
P1 Interface of CF card 50 /
J1 Interface of RBC/PLT sensor 3 Connect RBC/PLT

sensor
J2 Interface of WBC sensor 4 Connect WBC sensor
J3 Interface of HGB sensor 4 Connect HGB sensor
J4 +/-12V power interface of the analog 3 Connect +/-12V analog

circuit power
6-7
Hardware System
J5 120V power interface for aperture 3 Connect zapping

zapping power
J7 Interface of recorder 6 /
J8 JTAG interface for FPGA debugging 10 /
J9 Interface of LCD display 20 /
J12 RS232 serial port of LIS system 11 /
J13 BDM interface for CPU debugging 26 /
J14 Interface for door detector 2 /
J16 Interface of touchscreen control 24 /

board, indicator board and inverter
J17 Interface of power drive board 20 /
J18 +5V, +12V power interface of the 4 /

analog circuit
J20 Double-port USB-A interface 12 /
J22 Ethernet interface 10 /
J29 Single-port USB-A interface 6 /
J30 Single-port USB-A interface 6 /
6-8
Hardware System
J17 J14 J7 J16 J9 J8 J3 J4
J30
J29
J5
J20
J22
J2
J1
J12
J13 J18 P1
Figure 6-8 Position of the interfaces on the data board
6.2.3 Adjustment and Testing

All the adjustable parameters of the board can be adjusted by command. You can set up the
parameters on the software interface of the analyzer.
Function Definition of LEDs
See Table 6-2 for the function definition of data board LEDs.
Table 6-2 Function of data board LEDs
Indicator Function
+5V_POWER 5V power indicator of the digital circuit
+12V_POWER 12V power indicator of the digital circuit
FPGA_LED Indicator of FPGA logical work, it filters when the logical work goes on
normally
D3/D7/D8/D9 Indicator for software debugging
D17 -12V power indicator of the analog circuit
6-9
Hardware System
D20 +12V power indicator of the analog circuit
D21 -5V power indicator of the analog circuit
D22 +5V power indicator of the analog circuit
D38 Indicator for completing of FPGA configuration
D44 Indicator of network collision, flickers when collision occurs
D45 100M network indicator, flickers when transmitting data
D46 10M network indicator, flickers when transmitting data
D47 Indicator of network connection
D48 Indicator of the network full-duplex mode
D70 Power indicator of J30 single-port USB
D71/D72 Power indicator of J20 double-port USB
D73 Power indicator of J29 single-port USB
D100/D101 Connection indicator of J20 double-port USB
D102 Connection indicator of J29 single-port USB
D103 Indicator of USB-HUB chip connection
Function Definition of Test Points

See Table 6-3 for the function definition of data board test points.
Table 6-3 Function of data test points
Name or location
No. Description
of test points
1 VCC Digital 5V power
2 VDD Digital 3.3V power
3 D_1V8 Digital 1.8V power
6 D_1V25VRF Digital 1.25V power
7 D_1V25VTT Digital 1.25V power
6-10
Hardware System
8 D_1V2_FPGA Digital 1.2V power

9 TP36 5V power of USB interfaces
13 TP168 Voltage of button battery, 1.8V~4V
14 IRQ1 Interrupt signal when FPGA inputs to CPU
18 IRQ5 Interrupt signal when PCI-to-USB chip inputs to CPU
14 +12V Analog 12V power
15 -12V Analog -12V power
16 +5V Analog 5V power
17 -5V Analog -5V power
18 +56V 56V voltage of constant-current source
On/off control signal of constant-current source, the RBC/WBC
19 NCONST
constant-current source is on when electrical level is low
RBC constant-current source/zapping on/off control signal; zapping
20 NSEL_R when the electrical level is low, RBC constant-current source when it
is high
WBC constant-current source/zapping on/off control signal; zapping
21 NSEL_W when the electrical level is low, WBC constant-current source when
it is high
Zapping power on/off control signal, the zapping power is on when
22 NBURN
the electrical level is low
HGB lamp on/off control signal, the lamp is on when the electrical
23 NHLED
level is low
24 RBC1 RBC signal that has been amplified (level 1)
27 RBC Final output of RBC signal
28 RBCF RBC signal at ADC entry
29 PLT1 PLT signal that has been amplified (level 1)
30 PLT2 PLT signal that has been amplified (level 2)
31 PLT Final output of PLT signal
32 PLTF PLT signal at ADC entry
33 WBC1 WBC signal that has been amplified (level 1)
6-11
Hardware System
36 WBC Final output of WBC signal

37 WBCF WBC signal at ADC entry
HGB voltage that has been amplified (primary level), normally it is
38 HGB1
negative
39 HGB Final output of HGB voltage
40 VR_H ADC reference source voltage of HGB channel
41 VR_W ADC reference source voltage of WBC channel
42 VR_R ADC reference source voltage of RBC channel
43 VR_P ADC reference source voltage of PLT channel
44 HGND Digital ground
45 -12VM Monitoring voltage of analog -12V power
46 +12VM Monitoring voltage of analog +12V power
47 +56VM Monitoring voltage of 56V constant-current source
48 RHOLE RBC aperture voltage
49 WHOLE WBC aperture voltage
Note: refer to Table 6-3 for the location of all test points.
Definition of Keys
See Table 6-4 for the definition of keys on the data board:
Table 6-4 Definition of keys on the data board
No. Function
S12 Manual resetting of the system
6.2.4 Troubleshooting
Table 6-5 lists the common errors and troubleshooting methods of the data board from the
hardware point of view, the errors caused by software are not included. But many hardware errors
must be inspected by the software.
You need to check the following items before troubleshooting the errors:
1. Check if the wires are firmly connected to the data board;
2. Check if the wires are connected to the sockets of the same No., and if the wires are
cracked or damaged.
3. Check if the input power of socket L18 is normal (test +5V and +12V with a multimeter).
4. Check if the indicator of the data board is in normal condition according to Table 6-5.
After confirming all the above items are in normal condition, do as instructed in Table 6-5 to
troubleshoot the errors.
Table 6-5 Analyzing and handling data board errors
No. Phenomenon Judging criteria Solution
1 LCD blanks 1: Press the reset button S12 on the data board, if the Press S12
6-12
Hardware System
out error is cleared, it can be concluded as a program

failure; if not, do step 2.
Replug the
connection
2: Check if the data board if firmly connected to the
wires of the
inverter and LCD display. Replug the connection wires,
data board and
if the error is cleared, it can be concluded as an
the inverter
insecure connection error; if not, do step 3.
and LCD
display.
Replace the
connection
3: Replace the connection wires of the data board and
wires of the
the inverter and LCD display, if the error is cleared, it
data board and
can be concluded as a connection wire failure; if not, do
the inverter
step 4.
and LCD
display.
4Test the resistance of F4 with multimter, if the result is

Replace the
not 0, the error is caused by the protective tube F4; if
data board
not, do step 5.
5: Test voltage of the test point VDD with the multimeter,

if the result is not 3.135V~3.465V, U62 failure can be
concluded; if not, do step 6.
6: Test voltage of the test point D_2V5 with the

multimeter, if the result is not 2.375V~2.625V, U60
failure can be concluded; if not, do step 7.

multimeter, if the result is not 1.71V~1.89V, U63 failure
can be concluded; if not, do step 8.

multimeter, if the result is not 1.425V~1.575V, U61
9: Test voltages of the test points D_1V25VRF and

D_1V25VTT with the multimeter, if the results are not
1.188V~1.313V, U65 failure can be concluded; if not, do
step 10.
6-13
Hardware System
10: Test voltage of R27 with the multimeter, if the result

is 0V, then X6 failure can be concluded; if not, do step
11.

is 1.6V, but the voltage of R31 is 0V, then U11 failure

is 0V, then X2 failure can be concluded; if not, do step
13.
13: If D38 is off, reprogram correct version of FPGA

logic; if D38 is still off, then FPGA chip (U49) failure can
be concluded; if not, do step 14.
14: Replace LCD display and the inverter (display Replace the
assembly), if the error is cleared, then the display display
assembly failure can be concluded. assembly
Replug the
connection
1: Replug the connection wires of the data board and
wires of the
the inverter and LCD display, if the error is cleared, it
data board and
can be concluded as an insecure connection failure; if
the inverter
not, do step 2.
and LCD
display.
Replace the
connection
2: Replace the connection wires of the data board and
wires of the
LCD display the inverter and LCD display, if the error is cleared, it
2 data board and
flickers can be concluded as a connection wire failure; if not, do
the inverter
step 3.
and LCD
display.
3: Replace the data board, if the error is cleared, the

Replace the
display memory (U4) failure or crystal oscillator (X6)
data board
4: Replace the inverter and LCD display (display Replace the

6-14
Hardware System
Replug the
1: Check if the data board if firmly connected to the LCD
connection
display. Replug the connection wires, if the error is
wire of the data
cleared, it can be concluded as an insecure connection
board and LCD
error; if not, do step 2.
display
Replace the
2: Replace the connection wires of the data board and connection
the LCD display, if the error is cleared, it can be wire of the data
concluded as a connection wire failure; if not, do step 3. board and LCD
display
Spotted LCD
3
display
3: Check if there is solder joint on U6 weld, if yes, cut off
Cut off the
the solder joint when power is off. Reconnect the power,
solder joint
see if the error is cleared, if yes, the solder joint failure
with a knife.
4: Replace the data board, if the error is cleared, the Replace the
data board failure can be concluded; if not, do step 5. data board
5: Replace the inverter and LCD display (display Replace the

1: Check whether the IP set on the PC is in the same

network segment (10.0.0.x) as the data board, if not,
Set IP of the
reset the IP of the PC to 10.0.0.3. If the error is cleared,
PC to 10.0.0.3
then failure of incorrect IP address can be concluded; if
not, do step 2.
Fail to
3 connect 2: D47 and D48 is off when power is connected,
Replace the
network network chip (U36) failure can be concluded, or else, do
data board
step 3.
Replug or
2: D47 and D48 is on, poor connection of the network
replace the
wire, or the wire is damaged.
network wire
1: Check if the kernel software version of MCU and the

The USB Upgrade the
data board version are matched. View the kernel
interfaces kernel to the
5 version information by click "Service-> Version Info. " on
cannot be correct
the analyzer screen. If result is not matched, upgrade
used version.
the kernel to the correct version; or else, do step 2.
6-15
Hardware System
2: When power is connected, D70, D71, D72 and D73

Replace the
are not all on, USB power chip or PCI-to-USB chip
data board
failure can be concluded, or else, do step 3.

Replace the
are all on but hot, the U85 and U86 failure can be
data board
concluded, or else, do step 4.
4: When power is connected, D70, D71, D72 and D73 Replug the
are all on, replug the wire connected to the USB port. If wire connected
the error is cleared, it can be concluded as an insecure to the USB
connection error; if not, do step 5. port

Replace
are all on, replace devices connected to the USB port
devices
(mouse, keyboard or USB). If the replaced ones can be
connected to
used, then the device failure can be concluded; if not,
the USB port
do step 6.

are all on, test the USB port with a USB mouse or
Replace the
keyboard, if they can be used, but the USB printer
connection
cannot be used, check if the connection wire is marked
wire of printer
with 1P/28AWG+2C/24AWG, USB SHIELDED HIGH
with a qualified
SPEED, USB 2.0 VERSION. If not, replace the
one
connection wire of the printer. If the error is cleared, the
connection wire failure can be concluded.
1: Test voltage of pin 1 or pin 4 of X5 with the

Replace the
multimeter, if the result is 0V, then X5 failure can be
data board
The clock concluded; if not, do step 2.
resets each
6
time after 2: Cut off the power, test the voltage of capacitor C24
startup with the buttery on by a multimater, if the result is <1.8V, Replace the
then insufficient quantity of the battery can be button battery
concluded.
12V analog 1 Condition: the power wire is firmly connected. Replace the
voltage data board
2 If D20 is off, that indicates the data board failure; if
7 abnormal
not, do step 3.
3 Test voltage of the test point +12V or +12VM with the

multimeter, if the result is outside [11.412.6]V, then
6-16
Hardware System
data board failure can be concluded.
1 Condition: the power wire is firmly connected.
2 If D17 is off, that indicates the data board failure; if

not, do step 3.
-12V analog
Replace the
8 voltage 3 Test voltage of the test point -12V with the multimeter,
data board
abnormal if the result is outside [-11.4-12.6]V, then data board
failure can be concluded. or test voltage of the test point
-12VM, if the result is outside [2.282.52]V, it also data
board failure.
1 Condition: the power wire is firmly connected, and the

input power is normal.
56V voltage Replace the
9 2 Test voltage of the test point +56V or +56VM with the
abnormal data board
multimeter, if the result is outside [47.664.4]V, then
data board failure can be concluded.

input +/-12V power is normal.
Analog 5V 2 If D22 is off, that indicates the data board failure; if

Replace the
10 voltage not, do step 3.
data board
abnormal
3 Test voltage of the test point +5V with the multimeter,
if the result is outside [4.755.25]V, then data board
failure can be concluded.

input +/-12V power is normal.
Analog -5V 2 If D21 is off, that indicates the data board failure; if
Replace the
11 voltage not, do step 3.
data board
abnormal
3 Test voltage of the test point -5V with the multimeter, if
the result is outside [-4.75-5.25]V, then data board
failure can be concluded.
1. Condition: check if the power wire and the wire of Replace the
HGB sensor are firmly connected, and if +/-12V and HGB lamp
HGB blank +/-5V are under normal status. The error cannot be
10 voltage cleared by software operation and cleaning,
abnormal
2. See if the HGB lamp is off, if so, then the HGB lamp
failure can be concluded; if not, proceed with the
6-17
Hardware System
next step.
1 Test voltage of the HGB test point with the

multimeter, if the result is outside [3.2V4.9V], that
Replace the
indicates the ADC failure, the data board shall be
data board
replaced; if the result is outside [3.2V 4.9V],
proceed with the next step.
2 Enter the HGB gain calibration screen, modify HGB

gain manually, see if the HGB background voltage
changes accordingly. If the HGB background
Replace the
voltage changes, but is still lower than 3.2V when
HGB lamp
the gain has been modified to 255, that indicates
aging of the HGB lamp; the lamp shall be replaced.
Or else, proceed with the next step.
3 If HGB gain is modified, but HGB background

Replace the
voltage does not change, that indicates the data
data board
board failure.
6.3 Drive Board
6.3.1 Introduction
The drive board responds to the commands sent by the data board, controls the mechanical (e.g.
motor transmission assembly) and fluid components (e.g. valves and pumps) of the analyzer and
reports information like temperature and voltage to the data board. When controlling the
mechanical components, the drive board detects the position of the components (by
photocouplers, etc.), and when controlling the fluid components, it also monitors their pressure.
6.3.2 Structure
The drive board consists of the power supply, central control module, detection module and power
drive module.
6-18
Hardware System
Figure 6-9 Structure of the drive board

1 The power supply provides power to all the electrical components on the drive board, including
the input power of the drive board and the power that has been passed through the power chip on
the drive board.
2 The central control module communicates with the data board and schedules tasks.
3 The detection module collects information of temperature, pressure, voltage and fluid in tubing.
4 The power drive board drives the power components like motors, valves and pumps.
6.3.3 Sockets and Indicators
6-19
Hardware System
Figure 6-10 View of the drive board sockets and indicators
6.3.4 Test Points and Calibration Resistance
Figure 6-11 Test points and calibration potentiometer

After replacing the drive board or the rinse photocoupler assembly, the rinse photocoupler
calibration potentiometer must be readjusted per the following procedure:
1 Log in with service access level or above, select "Setup""Service Setup""Photocoupler

Calibration" to enter the screen; confirm whether the threshold needs to be modified.
2 Click "Start", the dialog box "Remove the Rinse tube from the container and press [OK] to
continue." will pop up, do accordingly and then click "OK".
3 A progress bar will be displayed; after the progress ends, a dialog box will pop up.
4 Do as instructed by the dialog box.
1) Adjust rinse photocoupler calibration potentiometer VR1 (see Figure 6-11) manually until
the rinse sensor calibration indicator D22 (see Figure 6-10) turns on.
2) Put the rinse tube back to the container (make sure there is rinse in the rinse container),
click "OK" to close the dialog box.
3) Then click "Finish".

5 The rinse priming progress bar will be displayed; after the progress ends, the new background
voltage, measured voltage and calibration status will be displayed; if the result is Fail, you need to
re-perform calibration from step 1.If the result is Pass, that means the calibration succeeded.
6-20
Hardware System
1) Checks before troubleshooting
Before troubleshooting the drive board, you should first check if the wires are firmly connected, if
they are connected to the sockets of the same No., and if the wires are damaged.
When you suspect that drive board failure occurs, check if the indicators are under normal status
per the following table.
Table 6-6 Status of drive board indicators
Indicator No. Normal Abnormal Description
If the indicator is off, that means the 5V

power supply is abnormal. Cut off the power
of the analyzer, test the resistance of the 5V
fuse (see Figure 6-11) with the multimeter, if
5V
D8 On Off the result is over 1 ohm, that indicates the 5V
indicator
fuse is damaged and the drive board needs
to be replaced; if not, check if the 5V output
voltage of the power board is within the range
[4.75V5.25V].
If the indicator is off ,that means the 3.3V

power supply is abnormal, which can be
caused by the abnormal 5V power supply or
3.3V
D11 On Off damaged drive board (you can determine
indicator
which the case is by observing the 5V
indicator or testing the voltage between VCC
and GND).
If the indicator is off, that means the 12V

power supply is abnormal, which will result in
the breakdown of all valves and pumps. Cut
off the power of the analyzer, test the
resistance of the 12V fuse (see Figure 6-1)
12V
D10 On Off with the multimeter, if the result is over 1
indicator
ohm, that indicates the 12V fuse is damaged
and the drive board needs to be replaced; if
not, check if the 12V output voltage of the
power board is within the range [11.4V
12.6V].
24V If the indicator is off, that means the 24V

D9 On Off
indicator power supply is abnormal, which will result in
6-21
Hardware System
the breakdown of all motors. Cut off the

power of the analyzer, test the resistance of
the 24V fuse (see Figure 6-11) with the
multimeter, if the result is over 1 ohm, that
indicates the 24V fuse is damaged and the
drive board needs to be replaced; if not,
check if the 24V output voltage of the power
board is within the range [22V30V].
If the indicator does not flicker, that means

the MCU is not working normally. First check
if the 3.3V power is under normal status
(voltage range [3.15V,3.45V]) by observing
the 3.3V power indicator or testing the
MCU Flickers Does not
D49 voltage between VDD and GND, if error of
indicator every 1s flicker
the 3.3V power supply is found, replace the
drive board or upgrade the MCU program of
the drive board per the upgrade instruction. If
the indicator still does not flicker after the
upgrade, the drive board must be replaced.
If the indicator does not flicker, that means

the FPGA is not working normally. First check
if the 3.3V power supply is normal (voltage
range [3.15V,3.45V]) by observing the 3.3V
indicator or testing the voltage between VDD
and GND. And check if the 1.5V power
FPGA Flickers Does not supply is normal (voltage range [1.45V
D51
indicator every 1s flicker 1.55V]) by testing the voltage between 1V5
and GND. If error of the power supplies is
found, replace the drive board or upgrade the
FPGA program of the drive board per the
upgrade instruction. If the indicator still does
not flicker after the upgrade, the drive board
must be replaced.
If all the indicators are confirmed to be ok, check if the MCU and FPGA versions are correct
on the software screen (Click the menu option"Service" "Version Info."); if yes, troubleshoot
the error as instructed in the following section.
2) Troubleshooting
You can roughly locate the error by the alarm, then accurately locate the error by its type and
dispose it, see Table 6-7.
Table 6-7 Troubleshooting methods
No. Type Description Troubleshooting Methods
6-22
Hardware System
1 Check if the power supply of the board is normal;
2 Check if the wires of motors and photocouplers

of all channels are firmly connected, if they are
connected to the sockets of the same No., and if
the wires are damaged.
This type of 3 After completing step 1 and 2, click "Clear Error"

errors include or perform self-test on the software screen to see if
but are not the error can be cleared.
limited to:
4 Check if the luminous surface of the
a Motor photocoupler is covered by dust or fluid spills, if so
breakdown; clean and reinstall the photocoupler to see if the
error can be cleared. If the error persists, replace
b Motor is
the photocoupler.
working, but
motor or 5 If the error cannot be cleared by replacing the
photocoupler photocoupler, replace the drive board.
Motors and error is reported;
1 6 If the error persists, replace the motor of the
photocouplers corresponding channel.
7 If the above step fails to clear the error, it may be

caused by the failure of mechanical components
(e.g. the friction force is too great), you should
handle it as a mechanical error.
1 Check if wires of the motors are firmly connected

and if they are damaged, if so, replug or replace
the wires when power is off.
2 Check if the fixing screws of the mechanical

Abnormal assembly are loose, if so, tighten the screws.
sounding of
motors 3 If the above problems are excluded, the error
may be caused by drive board failure, replace the
drive board.
4 If step 3 fails to clear the error, replace the motor

assembly.
1 First check if the valve can be open and closed

normally (you can hear a clatter) at the self-test
Breakdown of
screen; if yes, that means the valve drive is ok, you
2 Valves valves is
should consider the error as a fluidic error.
suspected
2 If the valves cannot be open and closed normally,
check if its wire is firmly connected or damaged. If
6-23
Hardware System
yes, replug or replace the wire.
3 If step 2 cannot clear the error, connect the wire

of another valve to the valve, then check if the error
lies in the valve drive circuit or the valve itself at the
self-test screen (if failure of valve 2 is suspected,
connect the wire of valve 3 to valve 2, then open
and close valve 3 at the self-test screen, if it can be
open and closed normally, that means the drive
board has been damaged and shall be replaced;
otherwise valve 2 is damaged and shall be
replaced).
1 Check if the pumps can work normally at the

self-test screen, if yes, the error shall be
considered as a gas path error.
a Abnormal
pressure is 2 If the valves cannot work normally, check if its
3 Pumps reported wire is firmly connected or damaged. If yes, replug
or replace the wire.
b Breakdown of
pumps 3 If the error cannot be cleared by step 2, replace
the drive board.
4 If the error persists, replace the pump.
1 Check if the indicators of the data board and

drive board are under normal status, if not, replace
the boards.
2 Check if the wire between the data board and

Communication drive board is firmly connected, if not, replug the
4 Communication
error is reported wire.
3 If the error cannot be cleared, replace the wire.
4 If the error persists, replace the drive board and

data board in turn.
1 Perform rinse photocoupler calibration to see if

the error can be cleared. If the calibration cannot
be perform successfully, replace the drive board
Rinse and then recalibrate.
Rinse
5 photocoupler
photocoupler 2 Disassemble the rinse photocoupler assembly,
error is reported
check if the photocoupler is cover by fluid spills or
dust, if yes, clean the photocoupler.
3 If the error cannot be cleared, replace the rinse
6-24
Hardware System
photocoupler assembly.
4 If the error persists, it may be caused by aging of

the rinse tube.
1 Check if the waste wire is loose or damaged, if

Incorrect alarm yes, pull off and clean the wire and then replug it,
6 Waste sensor or replace the waste wire.
of waste status
2 If no, replace the waste cap assembly.
a The aspirate 1 Check if the wires of the aspirate key and the
key does not sample compartment connection board are loose
respond when or damaged, if yes, replug or replace the wires.
being pressed.
7 Micro-switch 2 if the error cannot be cleared, disassemble the
b The sample switch of the aspirate key and the sample
compartment compartment connection board to see if they are
door cannot be covered by fluid spills. If yes, clean and then
closed. reinstall them.
6.4 Power Board
6.4.1 Introduction
In the BC-3600 Auto Hematology Analyzer, the power board provides 6 groups of stable power
output, including D5V, A+12V, A-12V, AC120V, P12V, P24V.
Section 6.4 is the servicing and troubleshooting guideline of the power board.
6.4.2 Structure
Function
The functional diagram of the power system is as follows:
6-25
Hardware System
Figure 6-12Functional diagram of the power board

Description:
The power board works under the 100-240V(10%) AC input voltage 50/60Hz(2Hz).Once the AC
power switch is on, all the circuits start to work, the D5V, A+12V, A-12V, AC130V, P12V, and P24V
all output.
The load of each circuit is listed in the following table:
Table 6-8 Properties of output voltage
Voltage Load
Minimum Normal Peak Range of Ripple&
Voltage regulatio regulatio
Load Load Load output voltage Noise
n factor n factor
D5V 1.5A 3A 5A 1% 3% 4.85~5.25V 100mV
A+12V 0.05A 0.3A 0.3A 1% 3% 11. 5~12. 5V 100mV
A-12V 0.01A 0.2A 0.2A 1% 3% -11.5~-12.5V 100mV
P12V 0.2A 2A 4A 1% 3% 11.5~12.5V 150mV
6-26
Hardware System
P24V 0A 0.3A 1.5A 1% 10% 22~30V 150mV

AC120V 0mA 0mA 60mA / / 110~140V(RMS) /
Output
11W 53W 123W
Power
Peak load means the Peak Load current of P12V and P24V in the cycle of 60S is no more
than 1S.
D5V has a single ground, A12V share one ground, P12V and P24V share one ground;
AC120V shall be alternate current(square wave), frequency is 50Hz+/-10Hz.
Definition of Interfaces
The power board has 6 outgoing interfaces, 4 of them (J1, J2, J3 and J4) are connected by
sockets; The AC input wires L and N are connected to external sockets, PCB No.s are L and N;
the inverse board is connected to the power board by a socket, PCB No. is PCBA1.See the
following figure for the position of each interface on the board.
Figure 6-13Mounting of the power board
See the following table for the function of each interface:
Figure 6-9 Input AC wires
Definition
L, live wire end of the power
N, neutral wire end of the power
Table 6-10Interface socket PCBA of power board and small board
PIN Definition
6-27
Hardware System
Connected to the positive end of the P24V

1/2
output voltage
Connected to the positive end of the P12V
3/4
output voltage
5/6/7/8 Connected to PGND end
9/10 Connected to AC1 end
11/12 NC
13/14 Connected to AC2 end
Table 6-11 Definition of output interface sockets
Socket
Name Socket Model
No.
D5V M32-032005-00, HEADER WTB 3.96mm DIP1*3SIDE VHseries J1
P12V, P24V M32-069002-00, HEADER WTB 4.2mm DIP2*3SIDE POWER J2

A+12V, A-12V M39-000139---, HEADER WTB 4.14MM DIP3SIDE J3
AC120V M33-022002-01, HEADER WTB 3.96mm DIP1*2SIDE J4

The following test points are important in the servicing process:
Table 6-12Definition of debug and test points
No. Location Function Reference Value
1 C123.+ PFC output voltage 39020V
2 U12.9 PFC chip reference voltage output 7.5V
3 C107.+ VCC voltage 17V~22.5V
4 C19 VDD voltage 121V
5 U1.8 Reference voltage of U1 5V
6 C111.+ Output voltage and ripple voltage of 50.5V

D5V
7 C117.+ A+12V output 120.5V
8 C115.- A-12V output -120.5V
6-28
Hardware System
9 C121. U3 power supply voltage 121V
10 U3.14 U3 reference voltage 5V
11 C119.+ P24V output voltage and ripple voltage 22~27V
12 C120.+ P12V output voltage and ripple voltage 120.5V
6.4.4 Replacing and Wiring Methods

Purpose: the power board is a critical component of the analyzer, it must be replaced and serviced
in time when related errors are found to ensure the proper running of the analyzer. Replace the
power board per the following procedure.
Tools: 107 cross-headed screwdriver, multimeter
Disassembling: 1 power off the analyzer and pull out the AC wire.
2 Take out the power supply assembly from the chassis of the analyzer.
3 Disassemble the power supply casing per the instructions in the operator's manual,
remove the 4 fixing screws of the power board and take out the board from the power supply
casing.
When servicing the boards, wear antistatic gloves.
When disassembling and installing the boards, cut off the power and pull out
the power wire.
Installing:
Do the above steps in reverse order
Verification: 1 Check if the ground wire of AC input socket on the casing is securely connected to
the conductive part of the analyzer chassis.
7 Connect the power wire and switch on the AC switch, the analyzer enters initializing status
and the indicators on the board turn on.
Make sure the power supply assembly and the analyzer chassis are fastened
by screws.
The disassembling operation must be done after the power board and the
surrounded parts cool down.
The errors of the power board can be troubleshooted per the following procedure.
6-29
Hardware System
Figure 6-14 Troubleshooting errors of the power board
6.5 USB Interface Board
6.5.1 Introduction
The USB interface board is extended to the front cover of the analyzer by extension wire to
provide users with a USB interface. The USB interface board does not need to be adjusted.
6-30
Hardware System
Figure 6-15 USB interface board
When the USB interface on the front cover cannot be used, do as follows:
1Check if the data board is working properly, if not, handle the problem of the data board first.
2Check if the wire connecting the USB interface board and the data board is loose or damaged,
if yes, replug or replace the wire; if not, check if the USB interface board is cover by fluid spills,
replace the board if so.
3If the problems mentioned in the above step are excluded, replace the data board.
6.6 Touchscreen Control Board
6.6.1 Introduction
Touchscreen control board is the interface of the touchscreen to the data board, it converts the
touch operations of users to signals recognizable to the data board. The touchscreen control
board does not need to be adjusted.
6-31
Hardware System
Figure 6-16 Touchscreen Control Board
No. Phenomenon Possible Cause Solution
Check if the data board is working

1
properly, if not, handle the problem of the
data board first.
Failure of 2 Check if the wire connecting the

touchscreen control touchscreen control board and the data
The touchscreen board is loose or damaged; if it is loose,
board/Failure of
1 does not respond replug the wire, if it is damaged, replace
touchscreen/the
to touch operation the whole front cover signal wire.
connection wire is
loose If the above problems are excluded,
3
replace the touchscreen control board.
If the error cannot be cleared, replace

4
the touchscreen.
When pressing The wire connecting Check if there is any crack on the
1
the touchscreen, the touchscreen touchscreen, if yes, replace the
the cursor can control board and touchscreen.
2 only move the touchscreen is
Replug the wire
2
horizontally or loose/the
touchscreen is
vertically cracked
The touchscreen is Perform touchscreen calibration.

1
The cursor cannot
not calibrated/the 2 If the error cannot be cleaned, check if
3 move to a certain
touchscreen is the touchscreen is cracked; if so, replace
area
cracked the touchscreen.
6.7 Volumetric Board
6.7.1 Introduction
The volumetric board detects the pressure of the pressure and vacuum chambers and measures
fluid volume. The board has been calibrated in factory, so you do not need to calibrate it before
using it.
6-32
Hardware System
Figure 6-17 Volumetric Board
Click "Service"
1 "System Test", and then click
the "WBC Test Time" and "RBC Test Time" buttons
on the screen to see if the error can be cleared.
Including but
not limited to the If the error cannot be cleared, check if the data
2
following errors: board is working properly; if not, resolve the problem
Abnormal of data board first.
WBC clog
1
1 detection
If data board error is excluded, check if the wires
3
of fluid WBC bubbles
2
connecting the volumetric board, the drive board and
RBC clog
3 the data board are loose or damaged; if the wires are
loose, replug them; if they are damaged, replace
RBC bubbles
4
them.
Check if there are bubbles in the WBC or RBC

3
tubes during analyzing process; if yes, clear the error
6-33
Hardware System
first.
Check if the fluid in the WBC or RBC tube is not

4
flowing or is flowing slowly; if yes, clear the error
first.
The analysis starting indicator shall turn on when

5
fluid reaches the analysis starting photocoupler, and
when fluid reaches the analysis ending photocoupler,
the analysis ending indicator shall turn on, then both
indicators turn off at the same time. If you did see the
above situation, replace the volumetric board.
Click
1 "Service"
"System Test", and then click
the Vacuum Of VC, Pressure Of VC and Pressure Of
PC buttons on the screen to see if the error can be
cleared.
If the error cannot be cleared, check if the data

2
board is working properly; if not, resolve the problem
of data board first.
1) Pressure
buildup fails Check if the detection channels of the vacuum and
3
Abnormal
2 pressure chambers are connected reversely; if yes,
pressure 2) Vacuum switch the two channels.
buildup fails
4 If the data board and detection channels
connection errors are excluded, check if the wires
connecting the volumetric board, the drive board and
the data board are loose or damaged; if the wires are
loose, replug them; if they are damaged, replace
them.
If the wires are ok, replace the volumetric board.

5
6.8 Sample Compartment Connection Board
6.8.1 Introduction
The sample compartment connection board is in the closed tube sampling assembly of closed
tube analyzers, it detects the open/close status of the compartment door and the type of tube
holder adapters. The sample compartment connection board does not need to be adjusted.
6-34
Hardware System
Figure 6-18 -Sample compartment connection board
The phenomenon of sample compartment connection board error is the analyzer does not
respond to closing operation. Clear the error per the following procedure:
1 Check if the drive board is working properly, if not, handle the problem of the drive board first.
2 Check if the wire of the sample compartment connection board (including J8 of drive board, J1
and the transfer socket of sample compartment connection board) is loose or damaged, if so,
replug or replace the wire.
3 If the problem mentioned in step 1 can be excluded, disassemble the board, check if there are
fluid spills in the switch; if yes, replace the board.
4 If not, replace the drive board.
6.9 Indicator Board
6.9.1 Introduction
The indicator board tells users the status of analyzer through sound and light.
Figure 6-19 Indicator board
6-35
Hardware System
6.9.2 Structure
The indicator board consists of indicators of three colors- red, yellow and green, their control
circuits and the drive circuit of buzzer. The indicator board does not need to be adjusted.
There are 7 LEDs for an indicator of one color. If the

indicator is dim, that means some of the 7 LEDs are
damaged. Disassemble the indicator board, see if the
The indicator is 7 LEDs are all on; if not, the board is damaged and
dim shall be replaced; if yes, test the voltage of pin 6 and
1 Indicator 7 of J1 on the board with the multimter, if the voltage
is lower than 4.5V, check if the 5V power supply of
the data board is normal.
The indicator 1 Check if the data board is working properly, if not,

cannot turn on handle the problem of the data board first.
2 Check if the wire of the board is loose or damaged;

The buzzer
if it is loose, replug it; if it is damaged, replace the
does not beep
whole front cover signal wire.
2 Buzzer when the
analyzer sends 3 If the problem of the wire is excluded, replace the
alarm indicator board.
6-36
7 Servicing
7.1 General
You can service the BC-3600 and BC-3300 as per Section 7.2 7.21. For the repairing with
verification, you must follow the procedures strictly to verify the maintenance.
7-1
Servicing
7.2 Disassembling the Panels
7.2.1 Opening the Front Panel Assembly

PurposeBath Assembly and Sampling Assembly are located behind the Front Panel, Screen
Assembly and Recorder are located on the Front Panel, to maintenance or disassemble the
parts mentioned above, need to follow the procedures to open the Front Panel Assembly.
ToolsN/A
Removal
1 Power off the analyzer and unplug the power cord from the wall outlet;
2 Resume the Sample compartment to work place (If the Sample compartment is already on
workplace, skip this step; for open vial configuration, also skip this step);
3 Pull the slot on right side(see Figure7-1) and open the Front Panel.
Figure 7-1 Opening the Front Panel Assembly
Make sure the door of sample compartment is closed when opening the
Front Panel, or it may cause damage on the sample compartment and the
Front Panel.
Installation: Install the Front Panel according to the steps stated above in reverse order.
Verification:
Pull the Front Panel to check whether its locked tightly;
Power on the analyzer and verify your replacement.
7-2
Servicing
7.2.2 Removing the Left Door

Purpose: Follow the procedure to remove the left door to disassemble the hardware parts
inside the analyzer
Tools: 107 cross-headed screwdriver
Removal:
1. Power off the analyzer and unplug the power cord from the wall outlet;
2. Open the Front Panel as per 7.2.1. For ease of operation, the opening angle should be no
less than 60;
3. Use the 107 screwdriver to unscrew the 2 cross-headed screws as per Figure 7-2;
4. Use the 107 screwdriver to unscrew the 3 cross-headed screws as per Figure 7-3, and then
remove the Left door.;
Figure 7-2 Inside of the left door Figure 7-3 Outside of the left door
Installation: Install the Left Door according to the steps stated above in reverse order.
Verification: Pull the Left Door to check whether its locked tightly.
7.2.3 Opening the Right Door

Purpose: Follow the procedures to open the right door to disassemble the fluidic parts inside
the right side of the analyzer as needed.
Tools: Flat-headed screwdriver
Removal: Insert the flat-headed screwdriver into the slot of the right door lock, and turn 90
counterclockwise and open the door(see Figure 7-4).
7-3
Servicing
Figure 7-4 Opening the Right Door
Installation: Install the Right Door according to the steps stated above in reverse order.
Verification: Pull the Right Door to check whether its locked tightly.
7.2.4 Removing the Top Cover

Purpose: Follow the procedures to remove the top cover to disassemble the Pressure
Chamber, Power supply assembly and cables.
Removal:
Power off the analyzer and unplug the power cord from the wall outlet;
Use the 107 screwdriver to unscrew the 3cross-headed screws on the back of analyzer as per
Figure 7-5;
Remove the Top Cover.
Figure 7-5 Removing the Top Cover
7-4
Servicing
Installation: Install the Top Cover according to the steps stated above in reverse order.
Verification: Check Whether the Top Cover is installed properly.
7.2.5 Removing the Front Panel Assembly

Purpose: Follow the procedures to remove the Front Panel Assembly, for the ease and space
of repairing or replacing the USB port inside.
Tools: Nipper pliers, 107 cross-headed screwdriver
Removal:
1. Turn off the analyzer and unplug the power cord from the wall outlet;
2. Open the Front Panel as per 7.2.1, unplug all cables connected between the Front Panel
and the Main unit;
3. Open the Left Door as per 7.2.2;
4. Use a sharp nose pliers to straighten the forelock on the hinge and then pull it out;
5. Lift the Front Panel Assembly for about 5mm, and then take out the assembly.
7-5
Servicing
Figure 7-6 Removing the Front Panel

Installation: Install the Front Panel Assembly according to the steps stated above in reverse
order.
Verification:
1. Check whether all components are installed and fixed in place;
2. Check whether the left and right side doors are locked tightly;
3. Check whether the front panel is locked tightly;
4. Power on the analyzer and verify your replacement.
7.3 Replacing the Hardware parts and Cable

connection
7.3.1 Replacing the Data board

Purpose: Follow the procedures to replace the data board as needed.
Removal:
2. Open the left door as per 7.2.2;
3. Unplug all cables on the power driver board;
4. Use the 107 cross-headed screwdriver to unscrew the 7 cross-headed screw
driver on power driver board(see Figure 7.7);
5. Take out the data board.
7-6
Servicing
Figure 7-7 Removing the Data Board
Installation: Install the Data board according to the steps stated above in reverse order
Verification:
1. Check whether the cables are connected correctly;
2. Power on the analyzer and verify the power and working indicators on the data board;
3. Execute Background test. If there is any error message, please do the
troubleshooting accordingly.
7.3.2 Replacing the Power Driver board

Purpose: Follow the procedures to replace the Power Driver board as needed.
Removal:
2. Open the Left Door as per 7.2.2;
3. Unplug the cables on the Power Driver board;
4. Use the 107 cross-headed screwdriver to unscrew the 6 cross-headed screws on
the Power driver board(see Figure 7-8);
5. Take out the Power Driver board.
7-7
Servicing
Figure 7-8 Removing the Power Driver Board
Installation: Install the Power Driver board according to the steps stated above in reverse order
Verification:
2. Power on the analyzer and verify the power and working indicators on the Power
Driver board;
3. Execute Background test. If there is any error message, please do the
troubleshooting accordingly.
7.3.3 Replacing the Touch screen control board

Purpose: Follow the procedures to replace the Touch screen control board as needed.
Removal:
2. Open the Front panel as per 7.2.1;
3. Use the 107 cross-headed screwdriver to unscrew the 2 screws fixing the
shielding cover;
4. Use the 107 cross-headed screwdriver to unscrew the 2 screws fixing the touch
screen control board;
5. Take out the Touch screen control board.
7-8
Servicing
Figure 7-9 Removing the Touch Screen Control Board
Installation: Install the touch screen control board according to the steps stated above in
reverse order
Verification:
2. Power on the analyzer and verify the replacement.
7.3.4 Replacing the Inverter

Purpose: When the Inverter is broken, display will have black screen trouble. In this case,
please follow the procedures to replace the Inverter.
Removal:
2. Open the Front Panel as per 7.2.1;
shielding cover, and then take out shielding cover;
Inverter, remove and keep the screws with gaskets;
5. Take out the Inverter.
7-9
Servicing
Figure 7-10 Removing the Inverter
Installation: Install the Inverter according to the steps stated above in reverse order
Verification:
2. Check whether the screws are fastened tightly;
3. Power on the analyzer and verify your replacement.
7.3.5 Replacing the USB Interface Board

Purpose: Follow the procedures to replace the USB Interface board as needed.
Tools: 107 cross-headed screwdriver, nipper pliers
Removal:
2. Remove the Front Panel as per 7.2.5;
shielding cover. Remove the Shielding cover;
Inverter and the grounding screw;
5. Take out the USB Interface board together with USB cable.
7-10
Servicing
Figure 7-11 Removing the USB Interface Board
Installation: Install the USB Interface board according to the steps stated above in reverse
order
Verification:
1. Check whether the cable is connected correctly;
2. Connect the mouse with the USB interface to check whether the cursor on the
display is moving normally. If not, please do the troubleshooting needed.
7.3.6 Replacing the Volumetric Board

Purpose: Follow the procedures to replace the Volumetric Board.
Removal:
1. Empty the fluidic system in the software. Power off the analyzer and unplug the
power cord from the wall outlet;
2. Open the Front Panel as per 7,2,1;
shielding cover, and take out the shielding cover;
Volumetric board;
5. Take out the Volumetric board.
7-11
Servicing
Figure 7-12 Removing the Volumetric Board
Installation: Install the Volumetric board according to the steps stated above in reverse order
Verification:
1. Check whether the cable connection between the Volumetric board and power
7-12
Servicing
driver board is correct. Check Whether the Pressure and Vacuum sensor are
connected correctly;
2. Turn on the analyzer. Check whether the background is within range, if not,
please do the troubleshooting needed.
7.3.7 Replacing the Sample compartment connection board

Purpose: Follow the procedures to replace the Sample compartment connection board.
Removal:
3. Take out the Tube holder adapter, and turn the Sample Compartment upside
down(see Figure 7-13);
4. Use the 107 cross headed screwdriver to unscrew the 2 M3x8 Cross-recessed
panhead screws fixing the cover(see Figure 7-14);
5. Use the 107 cross headed screwdriver to unscrew the 4 M3x8 Cross-recessed
panhead screws fixing the Sample compartment connection board, and unplug
the cables connected on the board. Take out the Sample compartment
connection board(see Figure 7-15).
Figure 7-13 Preparation before disassembling
7-13
Servicing
Figure 7-14 Disassembling cover of the micro-switch
1 -- Cover of micro-switch 2-- Cross-recessed panhead screw M3X8
Figure 7-15 Disassembling PCBA of sample compartment connection board
1 -- PCBA of Sample compartment 2 -- Cross-recessed panhead screw

connection board M3X8
Make sure the sample probe is always located on the upper position during
7-14
Servicing
the disassembly.
Installation: Install the Sample compartment connection board according to the steps stated
above in reverse order
Verification:
1. Check whether the cables are connected correctly between the Sample
compartment connection board and the Power driver board;
2. Turn on the analyzer and check whether the sample compartment is working
normally.
7.3.8 Replacing the Indicator board

Purpose: Follow the procedures to replace the Indicator board
Removal:
Shielding cover and remove it;
Indicator board;
5. Take out the Indicator board with cable.
7-15
Servicing
Figure 7-16 Removing the Indicator Board
Installation: Install the indicator board according to the steps stated above in reverse order.
Verification:
1. Check whether the cable connection between the Indicator board and the Data
board is correct;
2. Turn on the analyzer. Check whether the indicator can show red, yellow and green
colors correctly when error message displayed, and if the buzzer is working
normally.
7.4 Replacing the Power Supply Assembly
Purpose: Follow the procedures to replace the Power Supply Assembly as needed.
Removal:
2. Unplug USB cable, network cable and serial cable connectors on the power
fixing board;
3. Open the left side door as per 7.2.2; Remove the Top Cover as per 7.2.4;
4. Use 107 cross-headed screwdriver to unscrew the grounding screw of Power
supply assembly, unplug all the cable connection. Insert all the cables into the
analyzer;
5. Use 107 cross-headed screwdriver to unscrew the 4 cross-headed sunk screws
on the power fixing board;
6. Take out the Power Supply Assembly.
7-16
Servicing
Figure 7-17 Removing the Power Supply Assembly
Installation:
Verification:
1. Check whether the power connections meet the requirement;
2. Check whether the indicators on the data board and power driver board are on
good condition;
3. Check whether the monitoring voltages are within range in Self-test screen.
7.5 Replacing the Display Assembly
Purpose: Follow the procedures to replace the Display assembly as needed.

Removal:
3. Remove the touch screen control board and the shielding cover as per 7.3.3;
4. Use 107 cross-headed screwdriver to loosen the grounding screw(yellow and
green color) on the assembly;
5. Open the Left side door as per 7.2.2;
6. Remove the Top cover as per 7.2.4;
7. Disconnect the cables connected with the Display assembly from the Data
Board;
8. Use 107 cross-headed screwdriver to unscrew the fixing screws;
9. Take out the Display Assembly.
7-17
Servicing
Figure 7-18 Removing the Displaying Assembly
Installation: Install the Display assembly according the steps stated above in reverse order.
Verification:
1. Check whether all components are installed and fixed in place; Check whether
the cables are connected correctly;
2. Start up the analyzer and check the displaying of the screen.
7.6 Replacing the Touch Screen
Purpose: Follow the procedures to replace the Touch Screen as needed

Removal:
2. Remove the Display assembly as per the step2,3,8,9 of chapter 7.5. Note you do
not have to disconnect the cable;
3. Use 107 cross-headed screwdriver to loosen the plates fixing the Touch Screen;
4. Take out the Touch Screen.
Figure 7-19 Removing the Touch Screen
Installation: Install the Touch Screen according the step1-3 stated above in reverse order.
Verification:
1. Check whether all components are installed and fixed in place; Check whether
7-18
Servicing
the cables are connected correctly;

2. Start up the analyzer and check the touching and displaying of the screen.
7.7 Replacing the Recorder Assembly
Purpose: Follow the procedures to replace the Recorder Assembly as needed

Removal:
2. Open the Front Panel as per chapter 7.2.1;
3. Unplug all cable connection of the recorder include the grounding wire;
4. Open the door of the recorder;
5. Use the 107 cross-headed screwdriver to unscrew the 2 screws inside;
6. Pull the pins on both sides manually until the recorder is loose, and then take out
the recorder. Or open the cover, press the buckle (the grayed area) on the left of
the recorder at full tilt and then pull it out with one hand, when the buckle leaves
its original location, take off the recorder with another hand from outside of the
front cover.
7-19
Servicing
Figure 7-20 Removing the Recorder
Installation: Install the Recorder according the steps stated above in reverse order.
Verification: Start up the analyzer and check the printing of the recorder.
7.8 Replacing the valve, pumps, and other liquid

system parts
7.8.1 Replacing the valve

Purpose: Electromagnetic valve is used to control the liquid flow inside the tube, or
control the access way between different tubes, the failure modes usually are
clogged or rusty. In case of malfunction, please replace it in time. Following is
the detail steps.
Tool: 107 cross-headed screwdriver (107X75)
Removal:
1. Empty the liquid system;
2. Power off the instrument and unplug the power cable.
3. Open the right side panel (as per section 7.2.3), Disassemble the lyse
bottle and cap assembly.
4. Open the top panel(as per section 7.2.4);
5. Unplug the all the tubes on the valve;
6. Disconnect the cable connector between the fluidic diaphragm and
electric diaphragm;
7. As following Figure shows, Use the 107 cross-headed screwdriver to
remove the two M3 panhead screws
8. Take out the valve assembly.
7-20
Servicing
Please empty the liquid system before replacing the valve, otherwise the
reagents might leak out of instrument and cause the biological
contamination.
Figure 7-21 Electromagnetic valve disassembly

Installation: The installation procedures are the reverse order of step 1~8, during the
installation, the direction of the valve month should be in accordance with previous one.
Verification:
1. Check if the parts are assembled properly;
2. Make sure the tube connection and power cable connection are correct;
3. Turn on the instrument, and press Menu in counting screen to pop-up the
system menu, choose service-> Valve test to test the related valve.
7.8.2 Replacing the waste pump assembly

Purpose: Waste pump provides the power to the draining waste and establishing the
vacuum, it runs longer and more frequently, is a rapid wear part. In case of
malfunction, please replace it in time, following is the detail steps.
Removal:
3. Open the right side panel (as per section 7.2.3), Disassemble the lyse bottle and cap
7-21
Servicing
assembly.
5. Unplug the all the tubes connected with the waste pump, please dont disassemble
the fluid pump protecting plate;
6. Disconnect the cable connector between the fluidic diaphragm and electric
diaphragm
7. As following Figure shows, Use the 107 cross-headed screwdriver to remove the
four M3 sunk screws and four spacers.
8. Take out the waste pump assembly.
Please empty the liquid system before replacing the pump assembly,
otherwise the reagents might leak out of instrument and cause the biological
contamination.
Figure 7-22 Waste pump disassembly

installation, the direction of the pump month should be in accordance with previous one.
Verification:
1. Check if the parts are assembled properly or not;
2. Make sure the tube connection and power cable connection are
correct;
3. Turn on the instrument, and press Menu in counting screen to
pop-up the system menu, choose service-> system test to
access the Vacuum Test to test the vacuum.
7.8.3 Replacing the pressure pump and chamber assembly

Purpose: Pressure pump and chamber is used to establish and store the pressure, when
theyre broken, the pressure will be abnormal, thereby to influence the function
of bubble mixing and flushing. In case of malfunction, please replace the
7-22
Servicing
assembly in time, following is the detail steps.

Removal:
3. Open the right side panel(as per section 7.2.3), Disassemble the lyse bottle and
cap assembly.
5. Open the front panel assembly(as per section 7.2.1);
6. Unplug the all the liquid tubes and electric cable connected with the pressure
pump and chamber assembly;
7. As following Figure shows. Use the 107 cross-headed screwdriver to remove
the two M3 sunk screws fixing the assembly, take out the assembly from the
back of front panel;
8. As Figure 7-23 shows. Use the 107 cross-headed screwdriver to remove the
four M3 pan head screws fixing the pump, cut the tie fixed the pump, take out
the pump assembly;
9. As Figure 7-23 shows. Use the 107 cross-headed screwdriver to remove the
two M3 pan head screws fixing the chamber, remove the hoop and pad, then
take out the chamber;
7-23
Servicing
Figure e 7-23 Pressure pump and chamber disassembly

installation, the direction of the pump and chamber assembly and connection should be in
accordance with previous one.
Verification:
correct;
access the Pressure Test to test the pressure.
7-24
Servicing
7.8.4 Replacing the vacuum chamber assembly

Purpose: Vacuum chamber assembly is used to store the negative pressure for counting
and cleaning. In case of malfunction, please replace the assembly in time,
following is the detail steps.
Removal:
cap assembly.
4. Unplug the all the liquid tubes connected with the vacuum chamber assembly;
the two M3 pan head screws fixing the assembly, remove the hoop and pad
one by one, then take out the chamber;
Figure 7-24 Vacuum chamber disassembly
1 Vacuum Chamber Assembly 2 Pad

3 Hoop 4M3x8 pan head screw
installation, the direction of the pump and chamber assembly and connection should be
in accordance with previous one.
Verification:
7-25
Servicing

correct;
access the Vacuum Test to test the vacuum.
7.8.5 Replacing the volumetric tube assembly

Purpose: Volumetric tube is a key part, which is used to measure the liquid volume
during testing. If there are impurities or other particles, theyll give bad influence
to test result. Furthermore, if the optical sensor in the assembly is down, itll also
influence the regular service. In case of malfunction, please replace the assembly
in time, following is the detail steps.
Removal: The detail procedures of disassembly, please refer to Section 7.3.5
Installation: The installation procedures are the reverse order of disassembly
procedures, during the installation, please keep the top of the tube is the same with
previous one, and arrange the position of tubing properly.
Verification:
3. Turn on the instrument, disassemble the shielding cover of the volumetric
board, and then do background test, observe the liquid flow inside of the
volumetric tube, meanwhile care for the counting time. If the volumetric
sensor is normal, the instrument will return back to counting screen after
counting, otherwise itll give alarm.
7.8.6 Replacing the rinse detecting assembly

Purpose: This part is used to detect the volume of rinse. In case of malfunction, please
replace the assembly in time, following is the detail steps.
Removal:
3. Open the right side panel (as per section 7.2.3), disassemble the lyse bottle and
cap assembly.
4. Unplug the tube on the filter assembly;
the two M3 pan head screws fixing the filter, remove the rinse detecting
assembly;
7-26
Servicing
Figure 7-25 Rinse detecting unit disassembly
1 M3x6 pan head screws 2 Rinse detecting rack

3 Rinse detecting plate 4Sensor with connecting cable
5 Rinse detecting cover 6Filter assembly
Installation: The installation procedures are the reverse order of step 1~5.
Verification:
3. Turn on the instrument, go to counting screen, unplug the rinse cap assembly,
dont let the rinse go into the instrument. Count, observe if instrument could
7-27
Servicing
give the alarm. If it could reflect the real situation, that means the sensor
works well.
7.8.7 Replacing the isolation chamber

Purpose: Isolation chamber is used to filter the impurities and chippings from counting
bath. In case of malfunction, please replace the assembly in time, following is the
detail steps.
Removal:
1. Empty the counting bath;
2. Disassemble the shielding box of counting bath, do as Section 7.9.1;
3. Unplug the tube on the isolation chamber.
Installation: The installation procedures are the reverse order of above steps.
Verification:
3. Turn on the instrument to verify.
All the samples, controls, calibrators, reagents, wastes and areas contacted
them are potentially biohazardous. Wear proper personal protective
equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures
when handling them and the contacted areas in the laboratory.
7.8.8 Replacing the air filter

Purpose: Air filter is used to filter the air in the instrument, to prevent the impurities to
go into the tube and air pump. In case of malfunction, please replace the
Tools: flat-headed screwdriver
Removal:
1. Use the flat-headed screwdriver to rotate the lock on right door, to open the
right side door.(see Figure 7-26)
7-28
Servicing
Figure 7-26 Open the side door
2. The position of air filter is showed as following Figure;
Figure 7-27 Air filter
3. Remove air filter;

Installation: Insert the new air filter to the tube, and close the right side door.
Verification:
7.9 Replacing the counting bath assembly
7.9.1 Disassemble the shielding box assembly

Purpose: Shielding box is used to prevent interference during the counting process, it
should be disassembled, while replacing the counting bath assembly, following is
7-29
Servicing
the detail steps.

Removal:
4. Adjust the position of sample probe, keep the probe is out of the via hole of
the shielding box;
the four M38 pan head screws fixing the shielding box, take out the
shielding box;
Please adjust the sample probe position, before disassemble the shielding
box, make sure the sample probe is out of the shielding box to prevent the
probe is bended or hurt the operator.
Figure 7-28 Shielding box disassembly

1 Shielding box 2 M3x8 cross-headed pan head screws
Installation: The installation procedures are the reverse order of step 1~5, please make
sure not to press the liquid tube while cover the shielding box.
Verification:
7.9.2 Replacing the whole counting bath assembly

Purpose: This part is used to count sample. In case of malfunction, please replace the
7-30
Servicing

Removal:
4. Open the right side panel(as per section 7.2.3)
6. Disassemble the shielding box(as per section 7.9.1);
7. Unplug the tubes and cable connectors on the counting bath;
8. As following Figure shows. Use the 107 cross-headed screwdriver to loose the
four M36 pan head screws fixing the shielding box, but dont remove them;
9. Lift the counting bath about 5mm manually, make the four screws located in
the big hole, then take out the assembly.
Figure 7-29 Counting bath unit disassembly

Installation: The installation procedures are the reverse order of step 1~9, during
installation, hang the assembly on the four screws via the gourd hole, tighten the screws.
Then connect and arrange tubes. Make sure it s the same with previous, and never make
any tubes bended and pressed.
Verification:
7.9.3 Replacing the WBC counting assembly WBC

Purpose: This part is used to mix the sample and let sample react. In case of malfunction,
please replace the assembly in time, following is the detail steps.
7-31
Servicing
Removal:
the two M36 pan head screws fixing the counting bath, and remove the
counting bath cover board;
9. Take out the WBC counting bath.
Figure 7-30 WBC counting bath disassembly

1 --- Counting bath assembly 2 --- WBC assembly
3 --- Counting bath cover board 4 --- M36 cross-headed screw
installation, pay attention to the direction of the counting bath, Make sure it s the same
with previous, and never make any tubes bended and pressed.
Verification:
7.9.4 Replacing the HGB assembly

Purpose: HGB assembly is used to measure HGB. In case of malfunction, please
7-32
Servicing
Removal:
1. Disassemble the WBC assembly(as per section 7.9.3);
2. Use the cross-headed screwdriver to remove the two M2X10 screws,
disassemble the HGB assembly from counting bath.
Figure 7-31 HGB assembly disassemble

1 --- M222 cross-headed screw 2 --- HGB right part
3 --- O gasket 61 4 --- counting bath
5 --- HGB lift part
procedures.
Verification:
7.9.5 Replacing RBC counting bath assembly

Purpose: This part is used to mix the sample and let sample react. In case of malfunction,
Removal:
7-33
Servicing
the two M36 pan head screws fixing the counting bath, and remove the
counting bath cover board;
9. Use cross-headed screwdriver to remove the ground cable, take out the WBC
counting bath.
Figure 7-32 Disassemble the RBC counting bath

installation, pay attention to the direction of the counting bath, Make sure it s the same
with previous, and never make any tubes bended and pressed.
Verification:
7.9.6 Replacing the ruby aperture and rear bath

Purpose: This part is the flow path of the blood cell. In case of malfunction, please
Removal:
1. Disassemble the WBC assembly, and disassemble the HGB assembly, refer to
Section 7.8.4(If disassemble RBC aperture, please disassemble the RBC
assembly, refer to Section 7.8.5);
2. Use the cross-headed screwdriver to remove the two M3X10 screws,
disassemble the rear bath, ruby aperture and front bath.
7-34
Servicing
Figure 7-33 Disassemble the ruby aperture and rear bath

1 --- Rear bath electrode 2 --- O gasket 61
3 --- Rear bath 4 --- Fluorubber gasket
5 --- ruby aperture 6 --- front bath gasket
7 --- Front bath 8 ---Cross-headed screw M310
procedures. During installation, please pay attention to the direction of ruby aperture, the
big hole should be face to front bath, it can not reverse.
Verification:
7.10 Replacing the syringe assembly
Purpose: This part is used to dispense the fixed volume reagent or sample. In case of
malfunction, please replace the assembly in time, following is the detail steps.
Removal:
4. Open the right side panel (as per section 7.2.3), disassemble the lyse bottle and
cap assembly.
5. Unplug all the tubes on the syringe;
6. Unplug the ground cable of filter, and unplug the syringe motor cable
connector inside of instrument;
7-35
Servicing
the M3 pan head screws, remove the gasket, then take out the 10ml syringe
assembly or dual-syringe assembly.
Please empty the liquid system, before replacing the syringe assembly.
Otherwise, itll cause reagent leak or biological contamination. The syringe
assembly is a little heavy, during replacement, please support the syringe
assembly by hand, to prevent it drops and hurt people or instrument.
Figure 7-34 Disassemble the syringe assembly
1 Dual-syringe assembly 2 10ml syringe assembly

3 gasket 4M312 cross-headed screw
5 syringe grounding cable 6M312 cross-headed screw assembly
Installation: The installation procedures are the reverse order of disassembly steps
1~7. During installation, please make sure the connection and arrangement of the
syringe assembly tubes are same with previous.
Verification:
7-36
Servicing
7.11 Replacing the sample probe assembly
7.11.1 Replacing the whole sample assembly

Purpose: This assembly is used to aspirate and dispense the sample. In case of
Tools: 107 cross-headed screwdriver (107X75), positioning calibration fixture of sample
probe
Removal:
5. Open the right side panel (as per section 7.2.3), Disassemble the lyse bottle
and cap assembly.
6. Unplug the all the tubes connected with the sample probe assembly;
7. Disconnect the cable connector for the motor and sensors
8. As following Figure shows, Use the 107 cross-headed screwdriver to remove
all the M38 pan head screws fixing the drive belt and sample probe
assembly, then take out the whole assembly.
Figure 7-35 Disassemble the sample probe assembly

1 Sample probe assembly 2 M38 pan head screw
During installation, please care about the probe, to prevent the pricks. After
installation, please recalibrate the horizontal and vertical position.
7-37
Servicing
Installation: The installation procedures are the reverse order of installation

procedures, After installation, the horizontal and vertical position of sample probe needs
to be recalibrated.
Verification:
1. Go to Setup-> service setting-> adjustment, pop-up the screen as Figure
7-36 shows;
Constant level adjustment Constant level adjustment
of WBC bath sample of RBC bath sample
dispensing position dispensing position
Probe wipe constant

level adjustment
Figure 7-36 The screen to adjust the sample probe and wipe block
2. Adjust the wipe block:
For closed-reagent system: First, open the front panel(At this time, itll show
front panel is open error message in screen, to take care of it after
adjustment), see Figure-6B, Disassemble the tube baffle, remove the wipe
block, and assemble the positioning calibration fixture of sample probe on the
wipe block supporter by probe wipe fixing pin; Press wipe block position in
Y-direction motor as Figure 7-36 shows, then press start button, after
initialization, press up or down button to adjust the positioning calibration
fixture of sample probe, make its bottom is in the same level with sample
needle-point, see Figure-6C ; Then press save to save the setting; Remove
the fixture, and assemble the wipe block and probe wipe fixing pin, tighten
tube baffle to finish adjustment.
For the open-reagent system: open the front panel, remove the wipe block, and
assemble the positioning calibration fixture of sample probe on the wipe block
supporter by probe wipe fixing pin, the adjustment steps are same with above,
then remove the fixture, and assemble the wipe block to finish the adjustment.
7-38
Servicing
Tube baffle
Figure 7-37 Tube baffle
The bottom of the

fixture is on the
same level with the
sample probe tip
Figure 7-38 Sample probe is in the same level with the wipe block
3. Adjust WBC bath injection position: First, disassemble the shielding box, then
remove counting bath diaphragm as Figure 7-39 shows, Press WBC bath in
X-direction motor as Figure-6A shows. Then press start, press right to
move the sample probe to the right side, then place the WBC/RBC bath
calibration fixture properly and stably on the WBC bath as Figure 7-40 shows,
Press lift button in the software screen to move the probe just into the
aperture of fixture, then press save.
7-39
Servicing
Counting bath
diaphragm
Figure 7-39 Counting bath diaphragm
Figure 7-40 Calibrate WBC bath injection position
4. Adjust the RBC bath injection position: The same with above procedures,
Press RBC bath in X-direction motor to adjust the RBC bath injection
position and save the setting as Figure-6F shows.
7-40
Servicing
Figure 7-41 Calibrate the RBC bath injection position

5. Fix the counting bath diaphragm on the bath assembly, during the installation,
please make sure there is no any tube crimped.
6. Confirm the connection cable, tube, screws is assembled properly and tightly,
and keep the position of tube is the same with previous.
7.11.2 Replacing the vertical movement assembly

Purpose: Vertical movement assembly is used to drive and locate the sample probe in
vertical direction. In case of malfunction, please replace the assembly in time,
Tools: 2.5 Allen Key, Positioning calibration fixture of sample probe
Removal:
cap assembly.
5. Unplug the tubes and cable connectors on the sample probe assembly;
6. As following Figure shows. Use the 2.5 Allen key to remove the two M3 Allen
type socket screws, pull out the parallel guidance bar from lift side;
7. In the back of the panel, use the 2.5 Allen key to loose the M3 Allen type
7-41
Servicing
socket screws, then take out the vertical movement assembly in front.
Figure 7-42 Disassemble the vertical movement assembly

1 Parallel guidance bar 2 slide block
3 M36 Allen type stock screw
7-42
Servicing
Installation: The installation procedures are the reverse order of disassembly, before
tightening the two Allen type stock screws on slide block, move the horizontal part to
right side, and keep it is closed with the end face of slide block, then tighten the crews.
After installation, please recalibrate the position of sample probe.
Verification:
Please pay attention to the sample probe during installation, to prevent to be

pricked; after installing the vertical movement assembly, please recalibrate
the probe vertical movement.
During adjustment, please wait for the Menu to become the normal ground
color before operation, otherwise, the system is busy, please try again
later warning message will pop-up;
Please make sure the tube baffle is installed properly, otherwise there is a
risk to happen interference;
After counting bath diaphragm installation, please make sure the counting
bath diaphragm is not crimped.
7.11.3 Replacing the horizontal movement assembly

Purpose: Vertical movement assembly is used to drive and locate the sample probe in
vertical direction. In case of malfunction, please replace the assembly in time,
Removal:
3. Disassemble the whole sample assembly, steps and attention as Section 7.11.1
mentioned;
4. As following Figure shows. Use the 107 crossing screw driver to remove the
three M3 screws fixing the fluidic tube support assembly, take out the liquid
tube assembly;
5. Disassemble the vertical movement assembly, steps and attention as Section
7.11.2 mentioned;
6. Disassemble the sample probe assembly, steps and attention as Section 7.11.4
mentioned, to remove horizontal movement assembly.
7-43
Servicing
Figure 7-43 Disassemble the horizontal movement assembly

1 Sample probe assembly 2 Fluidic tube support
assembly
3 M38 crossing screw
Verification:
1. After installation, move the assembly repeatedly, make sure it moves smoothly,
there are not stagnation and noise;
7.11.4 Replacing the sample probe assembly

Purpose: Sample probe is an important part for sample collection, in case of attrition or
deformation, please replace the assembly in time, following is the detail steps.
Tool: 107 cross-headed screwdriver (107X75)Positioning calibration fixture of sample
probe
Removal:
2. Power off the instrument and unplug the power cable;
4. Unplug the all the tubes connected with the sample probe assembly;
5. As following Figure shows. Use the 107 crossing screw driver to remove the
two M3 screws fixing the pressure plate, take out the pressure plate;
7-44
Servicing
6. Disassemble the sample probe.
Figure 7-44 Disassemble the sample probe

procedures. During the installation, please pay attention to the direction of probe; After
installation, please re-calibrate the probe.
Verification:
7.11.5 Replacing the probe wipe fixing pin

Purpose: Probe wipe fixing pin is used to fix the probe wipe. In case of malfunction,
Tool: Nothing.
Removal:
7-45
Servicing
3. Pull the probe wipe fixing pin downward by hand as following Figure shows;
Figure 7-45 Disassemble the probe wipe fixing pin

Installation: Before installation, please install the sample probe and probe wipe in
advance, and lift up the probe wipe, let its top shore up the bottom of wipe supporter,
then push the probe wipe fixing pin to probe wipe along the underside of supporter,
make the circle of the pin is faced to the fillister in the probe wipe.
Verification:
7.11.6 Replacing the probe wipe

Purpose: Probe wipe assembly is used to clean the outside of the probe. In case of
Tool: Nothing.
Removal:
4. Unplug the all the tubes connected with the probe wipe;
5. Disassemble the sample probe assembly, steps and attention as Section 7.11.4
mentioned;
6. Disassemble the probe wipe fixing pin, steps and attention as Section 7.11.5
mentioned;
7. Pull out the probe wipe assembly.
7-46
Servicing
Figure 7-46 Disassemble the probe wipe assembly
1 Probe wipe fixing pin 3 Probe wipe assembly

2 Probe wipe supporter

procedures. During the installation, please pay attention to the direction of probe; After
installation, please re-calibrate the probe.
Verification:
7.12 Replacing the sample compartment assembly
7.12.1 Sample Compartment Assembly

Purpose: The sample compartment assembly can be removed or replaced as instructed
by this section.
Tool: 107 cross-headed screwdriver(107X75)
Removal:
7-47
Servicing

cap assembly. Unplug the all the cables connected with the sample probe
assembly;
5. Disconnect the cable connector for the motor and sensors;
6. Use the 107 cross-headed screwdriver to remove all the four M38 pan head
screws;
7. Take out the sample compartment assembly, take care of the cables.
Figure 7-47 Disassemble the sample compartment assembly

1 Sample compartment assembly 2 front panel
3 M3X8 screw
During disassembly and installation, please keep the sample probe always in
upper position.
Installation:
7-48
Servicing
1. When replacing the sample compartment assembly, the installation procedures

are the reverse order of above steps 1 to step 3;
2. Adjust the goodness of fit between the sample compartment door and front
panel.(Refer to Section 7.12.2)
Verification:
1. Check if all the cables(include motor) are connected well or not;
2. Close front panel, check the goodness of fit between the compartment door and
front panel;
3. Plug the power cable, turn on the instrument;
4. Perform initialization, observe the door to open and close;
5. Further check, go to Menu-> service, perform sample compartment many
times, to observe whether the door can open and close normally.
7.12.2 Sample compartment door

Purpose: The sample compartment door can be replaced or adjusted as instructed in this
section.
Tools: 107 cross-headed screwdriver(107X75), 2.5mm hex wrench.
Removal:
4. Use the 2.5mm hex wrench to remove four M38 pan head screws, take out
the sample compartment door.
Figure 7-48 Disassemble the sample compartment door

1 Sample compartment door 2 Door supporter
3 M3x8 screw 4M3x16 socket head screw
Installation:
7-49
Servicing
1. When disassembling the sample compartment door separately, install the door on the
assembly, then slightly screw the four M3x8 screws to fix the door, not too tight;
2. Close the front panel, move the door, make the upper gap and side gap almost same;
3. Open the front panel, make sure the compartment door not to be implicated;(please
hold the door by your hand)
4. Tighten the four M3x8 screws to finish the installation and adjustment;
5. When replacing the whole sample compartment assembly, loose the two M3x16
stock head screws fixing the door supporter;
6. Repeat the step 1 to step3;
7. Tighten the two M3x16 stock head screws and four M3x8 screws to finish the
installation and adjustment.
Figure 7-49 Adjust the sample compartment door

Verification:
1. Close front panel, check the goodness of fit between the compartment door and
front panel;
2. Plug the power cable, turn on the instrument;
3. Perform initialization, observe the door to open and close;
4. Further check, go to Menu-> service, perform sample compartment many
times, to observe whether the door can open and close normally.
7.12.3 Replacing the fixture of tube holder adapter

Purpose: The fixture of tube holder adapter can be replaced or adjusted as instructed in
this section.
Tools: 3mm hex wrench.
Removal:
4. Use the 3mm hex wrench to remove M4x10 socket head screws fixing the
card, take out the card.
7-50
Servicing
Figure 7-50 Fixture of tube holder adapter
1 Fixture of tube holder adapter 2 M4x8 socket head screw
Installation: Insert the fixture of tube holder adapter to corresponding card slot, and
tighten the M4x12 socket head screws.
Verification: Make sure all the parts are assembled properly.
7.12.4 Replacing the tube baffle

Purpose: The tube baffle can be replaced or adjusted as instructed in this section.
Tools: 107 cross-headed screwdriver(107X75)
Removal:
4. Use the 107 cross-headed screwdriver to remove two screws, take out the tube
baffle.
7-51
Servicing
Figure7-51 Disassemble the tube baffle

1 Tube baffle 2 screw
Installation: Locate the tube baffle as the position of screws, tighten the two screws.
Verification: Make sure all the parts are assembled properly.
7.12.5 Further disassembly for sample compartment assembly

Purpose: The closing door assembly, open-close detecting switch, driver motor and
flanged deep-slot ball bearing can be replaced as instructed in this section.
Tools: 107 cross-headed screwdriver(107X75), 2.5mm hex wrench, 1.5mm hex wrench.
Removal:
cap assembly.
5. Disassemble the sample compartment assembly;(as per section 7.12.1)
6. Use the cross-headed screwdriver to remove two M38 pan head screws,
disassemble the closing door assembly;
7-52
Servicing
Figure 7-52 Closing door assembly

1 Closing door assembly 2 pan head screw M3X8
Disassemble the driver motor:
1. Use the cross-headed screwdriver to remove two M38 pan head screws,
disassemble the closing door assembly;
2. Use the 1.5mm hex wrench to loose the two M3x5 screws to remove the
small synchromesh gear.
Figure 7-53 Disassemble driver motor
7-53
Servicing
1 Driver motor 2 Small band wheel MXL16

3 M3x5screw 4 pan head screw M3X8
Disassemble the open-close detecting switch:
1. Use the cross-headed screwdriver to remove four M38 pan head
screws, disassemble the driver step motor;
2. Use the cross-headed screwdriver to loose the two M38 pan head
screws to remove the open-close detecting switch with the supporter
together;
3. Use the cross-headed screwdriver to loose the two M38 pan head
screws to remove the open-close detecting switch from the supporter.
Figure 7-54 Disassemble the open-close detecting switch

1 Pan head screw M3X8 2 open-close detecting switch
3 supporter 4 Pan head screw M3X8
Disassemble the flanged deep-slot ball bearing:

1. Disassemble the flanged deep-slot ball bearing in left side(without motor).
Use the cross-headed screwdriver to loose the two M38 pan head screws
to remove the flanged deep-slot ball bearing;
7-54
Servicing
Figure 7-55 Disassemble the flanged deep-slot ball bearing in left side
1 flanged deep-slot ball bearing 2 pan head screw M3X8
5x14x3.5
2. Disassemble the flanged deep-slot ball bearing in left side(with motor). Use
the cross-headed screwdriver to loose the four M38 pan head screws to
remove the synchronous belt;
3. Use the 2.5mm hex wrench to loose the two M3x12 screws to remove the
big band wheel;
4. Use cross-head driver to loose the two M3x8 small pan head screws,
remove the right side flanged deep-slot ball bearing.
7-55
Servicing
Figure 7-56 Disassemble the right side flanged deep-slot ball bearing
1synchronous belt 2big band wheel
3 M3X12 Allen type stock screw 4 Pan head screw M3X8
5 flanged deep-slot ball bearing
5x14x3.5
Installation:
1. The installation procedures are the reverse order of disassembly;
2. The outside of the big band wheel should be parallel with the shaft surface;
3. Tighten the screws;
4. The tension of synchronous belt. Dont tighten the four screws fixing the motor at the
first, push the motor to force the synchronous belt tight(about 1kg force), then use the
cross-head driver to tighten the four screws.
Verification: The same with Section 7.11.1.
7.13 Replacing the start key
Purpose: The start key can be replaced or adjusted as instructed in this section.
Tools: 2.5mm hex wrench;
Removal:
cap assembly.
7-56
Servicing
4. As following Figure shows, Use the 2.5mm hex wrench to remove two M36
Allen type socket screws, take out the start key;
Figure 7-57 Disassemble the start key

Installation: Please put the start key in front and the lower end faces to the limit-lead.
Verification: Check whether the start key can move normally without any barriers. And
check the gap between start key and front panel, it should be uniform.
7.14 Micro-Switch Assembly Replacement
Purpose: Need replacement when micro-switch is faulty.

Tools: 107x75 Cross screw driver
Removal: 1. Turn-off main unit, and plug out power cable
2. Open the front panel, the related steps and notice as 7.2.1 indicates.
3. Open Right panel, the related steps and notice as 7.2.3 indicates.
4. As Figure 7-58, loose two M2x10 cross-head screws with 107x75 cross
screw driver and take down the screws.
7-57
Servicing
Figure 7-58 Disassemble Micro-switch
1 M210 cross pan-head screw 2 micro switch

3 Press Button
Installation: Installation procedure is reverse steps to disassemble.
Verification: No stuck with press button, meantime, check the gap between button and
panel, make sure its consistent.
7.15 Fluidic Support-bracket Assembly
Purpose: Need replacement when the tubing support-bracket faulty.

Tools: 107x75 cross screwdriver
Removal: 1. Run drain program firstly.
2. Power-off the main unit and plug out power cord.
3. Open the front panel, the steps and notice as 7.2.1 indicates.
4. Pull out the tube connected to sample probe.
5. As Figure 7-59, take out three M3x8 stainless cross pan-head screws
assembly with 107x75 cross screw driver, and take out tubing assembly.
7-58
Servicing
Figure 7-59 Disassemble Fluidic Support-bracket
1 Sampling assembly 2Tubing Support-bracket

3 M38 stainless cross pan-head screw
assembly
Installation: Installation procedure is reverse steps to disassemble
Verification: Make sure all parts well installed and fixed, and tubing is smooth.
7.16 Front Panel Detection Assembly
Purpose: Detection assembly is used to detect if the front panel is open or not, when its
defective, need to replace.
Tools: 107x75 cross screw driver
Removal1. Power-off the main unit and plug out power cord.
3. Open the top panel, the steps and notice as 7.2.4 indicates.
4.As figure 7-60, loose the three M3x6 cross countersunk head screws,and
take down the open-close detection assembly.
7-59
Servicing
Figure 7-60 Disassemble front panel detection assembly
1 Front panel detection assembly 2 Front board

3 M3 6 cross countersunk head
screws
Installation: Installation procedure is reverse steps to disassemble
Verification: Repeatedly press detection assembly of front panel and make sure move
smooth and no stuck.
7.17 Ambient Temperature Sensor Replacement
Purpose: When the Temp. Sensor is defective, it need replacement.

Tools: Plier or wrench
Disassembly: 1. Power-off the main unit and plug out power cord.
3. Open the top panel, the steps and notice as 7.2.4 indicates.
4. Take out M5 screws with pliers or wrench, which used to fix temp.
sensor.
5. Take out ambient temperature sensor and connection cable.
Installation: Installations reverse steps to disassemble, please notice not to ignore rubber
o-ring in installation procedure.
Verification: Please verify the temperature sensor is reliable and the indicated
temperature is correct.
7-60
Servicing
7.18 Cap Assembly
Purpose: When reagent cap assembly is broken,

Tools: None
Removal (Lyse Cap Assembly):
1Please run drain program firstly
2. Please plug-out power cord after power-off
3. Open the right panel door, the steps and notices as 7.2.3 indicates.
4. Please take out orange connecter in counter-clockwise, and move up the
connecter and tubes in case of some left fluidic drops.
5. Then loosing cap assembly in counter-clockwise, then pull up the whole lyse
cap assembly.
Figure 7-61 Lyse Cap Assembly Disassembly
Diluent Cap Assembly, Rinse Cap Assembly, Waste Cap Assembly:

Removal:
1. Run the drain program firstly;
2. Please plug out power cord after turn-off;
3. Please loose the cap assembly related colored connectors in counter-clockwise
(waste cap assembly need to loose BNC connecter); Diluent cap connecter is
green, Rinse cap connecter is blue , the waste cap connecter is red;
4. Loose the cap and pull up the whole assembly.
Installation: Please insert the tube into reagent bottle firstly, and tighten the connecter
and cap in clockwise (waste cap need to tighten BNC connecter as well) .
Verification: Make sure the assembly is well tightening, no kinkled tube, and run some
tests to verify.
7-61
Servicing
The reagents are irritating to eyes, skin and diaphragm. Wear proper
personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe
laboratory procedures when handling them in the laboratory.
If the reagents accidentally spill on your skin, wash them off with plenty of
water and if necessary, go see a doctor; if the reagents accidentally spill into
your eyes, wash them off with plenty of water and immediately go see a
doctor.
When loose the Cap connecter, and be careful with left liquid drops on the
instrument or table.
Please tighten the reagent cap in case of the reagent contaminated when the
reagent and analyzer was connected.
7.19 Gain Adjustment
Purpose: Gain Setup, to ensure instrument work in defined status.

Adjustment Method: 1. With service login, press Menu Setup Gain Setup to enter
following Gain Setup screen as shown in Figure 7-62.
7-62
Servicing
Figure 7-62 Gain Adjustment Screen
2Only the edit is activated, you can adjust the gain value. If the gain setup
exceeding the effective range or the value is empty, the system will
give Input invalid, and recover the values back to original.
3After input the valves, you can press the menu or other figure button, it
will pop up following dialogue. Press Yes, Save the setting and switch
the screen; click No to exit the dialogue without save.
Figure 7-63 Save Screen

Caution:
1Before Setup, please press Menu Service Maintenance Prime
All ReagentsAfter finish prime, press Menu Setup Service
Background data monitoring, then go to background data monitoring
screen, press background screen, and press background button, execute
daily check, and observe if there are bubbles in RBC bath during courting
process?
2As shown in figure 7-64, please check if WBC bath full with reagents and
HGB light is turned on, meanwhile observe if any bubble in inner bath wall.
if it is, please make trouble-shooting, then input the new gain value and make
the HGB blank voltage around 4.50.2V , then save to exit.
7-63
Servicing
Figure 7-64 WBC counting bath and HGB assembly (Right panel)
7.20 Motor Type, Position and Different Application List
No Motor Type Position Application

Horizontal Movement Drive the sampling assembly to move in
1 Step motor
Assembly horizontal direction
Drive the sampling assembly to move in
Vertical Movement
2 Step motor vertical direction and accomplish puncture,
Assembly
sampling and sample dispense.
Sampling Door Accomplish the sampling door open-close
3 Step motor
Assembly action.
7.21 The Check List After Material Replacement
No Material Description Steps to Replace and Verify
1 Empty fluidics and shut down the analyzer

2 Replace the valve and start up the analyzer
Home-made three-way
according to standard procedures
1 electromagnetic valve
3 Make sure there is no leakage in tube connections
service kit
4 Go to the valve test screen to verify if the valve works
properly
7-64
Servicing
1. Empty fluidics and shut down the analyzer

2. Replace the valve and start up the analyzer
Home-made two-way
2 electromagnetic valve
3. Make sure there is no leakage in tube connections
service kit
4. Go to the valve test screen to verify if the valve works
properly
2 Replace the tube and start up the analyzer according
to standard procedures.
3 Make sure there is no leakage in tubing connections
and no liquid suspending on the wall of the
Volumetric Tubing
3 volumetric tube
Assembly
4 Set up the volume of the volumetric tubing at the
corresponding screen
5 Monitor the background information.
6 Calibrate in the whole-blood mode
7 Calibrate in the predilute mode
4 Filter N/A
6. Empty fluidics and shut down the analyzer. Replace
the bath, and then start up the analyzer according to
standard procedures, make sure the tubes are
connected properly and there is no leakage
7. Perform gain calibration
8. Verify the HGB and MCV values, as well as the
lymphocyte peak value in the WBC histogram after
gain calibration
9. Monitor the background information
5 WBC bath assembly 10. Calibrate in the whole-blood mode
11. Run the calibrator after finishing calibration, and
verify the result according to the specifications in the
PRS
12. Calibrate in the predilute mode
PRS
14. Test repeatability using normal-level control in the
whole-blood mode and predilute mode
standard procedures, make sure the tubes are
6 RBC bath Assembly connected properly and there is no leakage
3. Verify t MCV values, as well as RBC histogram after
gain calibration
7-65
Servicing

5. Calibrate in the whole-blood mode
PRS
PRS
Test repeatability using normal-level control in the
whole-blood mode and predilute mode.
the aperture, and then start up the analyzer
according to standard procedures. Make sure the
tubes are properly connected and there is no
leakage
3. Verify the HGB and MCV values, as well as the peak
values in the WBC histogram after gain calibration
Aperture sensor
7 5. Calibrate in the whole-blood mode
(D80um)
PRS
PRS
standard procedures. Make sure the tubes are
properly connected and there is no leakage
3. Verify the HGB and MCV values, as well as the peak
WBC bath service parts values in the WBC histogram after gain calibration
8
(without aperture) 4. Monitor the background information
5. Calibrate in the whole-blood mode
PRS
7-66
Servicing

PRS
1 Empty fluidics and shut down the analyzer. Replace
2 Perform gain calibration
3 Verify the MCV values, as well as RBC histogram
after gain calibration
4 Monitor the background information
RBC bath service parts 5 Calibrate in the whole-blood mode
9
(without aperture) 6 Run the calibrator after finishing calibration, and
PRS
Calibrate in the predilute mode
7
8 Run the calibrator after finishing calibration, and
PRS
9 Test repeatability using normal-level control in the
3 Verify the HGB and MCV values, as well as the peak
WBC bath service parts 5 Calibrate in the whole-blood mode
10
(without HGB) 6 Run the calibrator after finishing calibration, and
PRS
7
PRS
9 Test repeatability using normal-level control in the
11 Bath Washer Service Parts
standard procedures
2 Make sure the tubes are connected properly and
7-67
Servicing
there is no leakage
PRS
8
Run the calibrator after finishing calibration, and
9
PRS
2 Replace the syringe and start up the analyzer
there is no leakage
2.5ml home-made syringe
12 PRS
(fluorine rubber)
6
PRS
8 Test repeatability using the normal-level control in the
whole-blood mode
predilute mode
the syringe, and then start up the analyzer according
to standard procedures
there is no leakage
3 Remove bubbles in the 100ul syringe
100L syringe service 4 Calibrate in the whole-blood mode
13
assembly 5 Run the calibrator after finishing calibration, and
PRS
6
PRS
7-68
Servicing

whole-blood mode
predilute mode
the syringe, and then start up the analyzer according
there is no leakage
14 10ml home-made syringe PRS
5
PRS
whole-blood mode
predilute mode
2 Replace the isolation chamber, and then start up the
analyzer according to standard procedures. Make
15 Isolation chamber sure that the waste can be drained through the
isolation chamber
there is no leakage
2 Replace the probe and start up the analyzer
3 Refix the position of the probe wipe
PRS
16 Open-vial sample probe
6
PRS
whole-blood mode
predilute mode
7-69
Servicing

2 Replace the probe wipe and start up the analyzer
3 Make sure there is no leakage in the new probe wipe
PRS
17 Probe Wipe
6
PRS
whole-blood mode
predilute mode
2 Replace the probe and start up the analyzer
3 Refix the position of the probe wipe
PRS
18 Piercing probe
6
PRS
whole-blood mode
predilute mode
1 Perform rinse priming, and make sure that the rinse
can be aspirated into the analyzer
19 Rinse cap assembly
2 No leakage around the rinse connector in the rear
panel
1 Perform lyse priming, and make sure that the lyse
can be aspirated into the analyzer
20 Lyse cap assembly
2 No leakage around the lyse connector in the back
panel
1 Perform diluent priming, and make sure that the
diluent can be aspirated into the analyzer
21 Diluent cap assembly
2 No leakage around the diluent connector in the back
panel
7-70
Servicing
1 Remove the BNC connector of the waste cap

Waste cap assembly (drain assembly in the back panel, and check if the waste
22
waste to waste container) container full error is reported. Put the connector
back, and the error is removed
1 Make sure that the fluidic pump is working properly
and there is no leakage
23 Fluidic pump assembly
2 Go to the system test screen to test the vacuum
pressure, and check if the result is OK
1 Go to the system test screen to test the pressure of
24 Air pump assembly
the pressure chamber, and check if the result is OK
1 Empty fluidics first
2 Replace the rinse detection assembly, and then
25 Rinse detection assembly
adjust the potentiometer on the driver board
3 Go to the adjustment screen to make adjustment
26 Vacuum chamber assembly N/A
Rubber pipe. 3/32"X5/32",
S-50-HL(replace with the
27 2 Replace the pipe and start up the analyzer according
wider rubber pipe of the
towline)
Rubber pipe.1/16"X1/8",
S-50-HL(replace with the
28 2 Replace the pipe and start up the analyzer according
narrower rubber pipe of the
towline)
Rubber pipe. PTFE,
1/32"X1/16",30 (0.78 1 Empty fluidics and shut down the analyzer
29 TEFLON; replace with the 2 Replace the pipe and start up the analyzer according
0.78 TEFLON pipe of the to standard procedures
RBC isolation chamber)
Rubber pipe. PTFE,
0.066"X0.098"X (1.5 1 Empty fluidics and shut down the analyzer
30 TEFLON; replace with the 2 Replace the pipe and start up the analyzer according
T20 storage pipe between to standard procedures
V4 and V12)
Rubber pipe OD3mm
ID1mm EVA (narrow EVA, 1 Empty fluidics and shut down the analyzer
31 sampling pipe, use the pipe 2 Replace the pipe and start up the analyzer according
connecting the towline and to standard procedures
the sample probe)
CF card (1G) service kit 1 Export parameters
32
(with software) 2 Restore parameters
1 Export parameters
2 Replace the data board and start up the analyzer
33 Data board
3 Restore parameters
7-71
Servicing
1 Export parameters
2 Replace the data board and start up the analyzer
34 Driver board
3 Restore parameters
1 Connect to the power supply, and make sure that you
can start up and shut down the analyzer by pressing
35 Power board the power button
2 In the system status screen, make sure all the
voltage values are in specified ranges
1 Make sure that the interval between the shielding
cover of the interface board and the side door of the
circuit is even
36 Interface board 2 Test all the USB interface with the USB interface of
the keyboard to make sure all the interfaces function
properly
3 Test the serial interfaces
the volumetric board, and then start up the analyzer
2 Check and make sure the pressures of the vacuum
chamber and pressure chamber are OK
37 Volumetric metering board
PRS
6
PRS
whole-blood mode
predilute mode
1 Open the front cover, check if the indicator is red and
38 Indicator Board
the buzzer buzzes
Sample compartment 1 Go to the system test screen and perform the sample
39
connection board compartment test. Make sure the test result is OK
TR60-D recorder driver 1 Print at the count screen and make sure the printout
40
board is OK
41 Touch Screen 1 Calibration on Touch Screen after replacement
1 Make sure the analyzer can be started up and the
42 Inverter
screen displays properly
43 Photocoupler and its 1 Run a background cycle and make sure the sampling
7-72
Servicing
connecting cables (the assembly functions properly

vertical photocoupler of the
sampling assembly)
1 Empty fluidics and shut down the analyzer. Start up
the analyzer according to standard procedures after
replacing the HGB. Set up the HGB blank voltage
gain
44 HGB assembly verify the result according to the specifications in the
PRS
6
PRS
whole-blood mode
predilute mode
1 Print at the count screen and make sure the printout
45 Recorder
is OK
1 Adjust the interval between the compartment and the
cover
Sample compartment 2 Test the sample compartment in the system test
46
assembly screen, and make sure the result is OK
3 Adjust the position of the sample probe relative to the
tube in the sample compartment
Sample compartment door
47 1 Adjust the interval between the door and the cover
1 Test the sample compartment in the system test

48 Door secure assembly
screen, and make sure the result is OK
1 Fix the position of the sample probe wipe
2 Adjust the position of the sample probe in the
WBC/RBC bath and the sample compartment
49 Sampling assembly
PRS
5
7-73
Servicing
PRS
whole-blood mode
predilute mode
1 Fix the position of the sample probe wipe
2 Adjust the position of the sample probe in the
WBC/RBC bath and the sample compartment
PRS
50 Vertical motion assembly Calibrate in the predilute mode
5
PRS
whole-blood mode
predilute mode
Fluidic Pipe Bracket 2 Replace the assembly and start up the analyzer
51
Assembly according to standard procedures. Make sure there is
no leakage
1 Empty fluidics first. Replace the syringe, and then
start up the analyzer according to standard
procedures
there is no leakage
52 Double Syringe Assembly
PRS
6
PRS
whole-blood mode
predilute mode
Double Syringe Drive 1 Empty fluidics first. Replace the syringe, and then
53
Assembly start up the analyzer according to standard
7-74
Servicing
procedures
there is no leakage
PRS
6
PRS
whole-blood mode
predilute mode
procedures
there is no leakage
54 10ml Syringe Assembly PRS
5
PRS
whole-blood mode
predilute mode
procedures
there is no leakage
Single Syringe Drive
55 3 Calibrate in the whole-blood mode
Assembly
PRS
5
7-75
Servicing

PRS
whole-blood mode
predilute mode
1 Start up the analyzer and make sure the screen
56 Screen assembly
display is OK
57 Adapter Assembly 1 N/A
58 Adapter Assembly 2 N/A
1 Connect to the power supply, and make sure that you
can start up and shut down the analyzer by pressing
Power Supply Assembly
59 the power button
2 In the system status screen, make sure all the
voltage values are in specified ranges
1 Make sure the key can sink and resile smoothly and
60 Aspirate Key the probe functions properly while the aspirate key is
pressed
1 Make sure there is alarm sound while the front cover
Front Cover Status
61 is open and the error can be removed manually after
Detection Assembly
the cover is closed
1 Start up the analyzer according to standard
procedures. Click the "Zap" button at the
62 Power Cord for Zapping
maintenance screen, and make sure you can hear
the hiss while zapping
Sample Compartment 1 Start up the analyzer according to standard
63 Micro-switch Switching procedures. Test the sample compartment at the
Cable system test screen, and make sure the result is OK
Valve Control Connecting
64 procedures. Perform valve test and make sure all the
Wiring
valves work properly
procedures. At the system test screen: click "ASP/LS
Motor Control Connecting
motor", "DIL motor", "X motor" and "Y motor" in turn,
65 Wiring (closed model)
and make sure the test results are OK
2 Test the sample compartment in the system test
screen, and make sure the result is OK
7-76
8 Maintaining your analyzer
8.1 General Guidelines
Maintenance Period Content of Maintenance
Every day If you are to use the analyzer 24 hours a

day, be sure to perform the probe cleanser
cleaning procedure everyday.
To get reliable result, run QC program
everyday before sample test. See chapter 8
QC in operation manual for details.
Every week If you shut down your analyzer every day
and follow the specified shutdown procedure to
do that, you need to perform the Probe
cleanser cleaning procedure every week.
As needed When you think the baths might be
contaminated, perform the Clean the baths
procedure.
For closed tube model, the tube baffle
should be cleaned regularly to avoid possible
biohazards. See Cleaning the Tube Baffle in
operation manual.
When the analyzer reports bubbles in
volumetric tubes frequently, you need to check
if the air filters are covered by dust; if so,
please replace the air filters.
For closed tube model, it is recommended
that you change the pierce needle whenever
30,000 samples have been analyzed.
When this analyzer is not to be used for
two weeks, be sure to perform the Prepare to
ship procedure to empty and wash the fluidic
lines and then wipe the analyzer dry and wrap
it up for storage.
If you see other error messages, see
chapter troubleshooting in operation manual,
for solutions.
8-1
Maintaining your analyzer
8.2 Operation guidance of Quality Control and Calibration
8.2.1 Quality control

Mindray recommends you run the QC program daily with low, normal and high level controls. A
new lot of controls should be analyzed in parallel with the current lot prior to their expiration
dates. This may be accomplished by running the new lot of controls twice a day for five days
using any empty QC files. The QC files calculate the mean, standard deviation and coefficient
of variation for each selected parameter. The instrument-calculated means of these ten runs
should be within the expected ranges published by the manufacturer. See Chapter Using the
QC programs in operation manual.
8.2.2 Calibration
Calibration is a procedure to standardize the analyzer by determining its deviation under
certain specified conditions. In order to get accurate sample analysis results, you should
calibrate the analyzer per the procedure below when necessary. See Chapter Using the
calibration programs in operation manual.
8.2.3 Guidance for mixing skills of Mindray controls and calibrations

Daily storing
It is recommended that controls and calibrations are stored in refrigerator with temperature
2~8.
Notes
1. Only use the controls and calibrations within expiry date. After being taken out of the
refrigerator, the controls and calibrations should be placed in environment with room
temperature for 15mins before mixed or used.
2. Mark the open-box date on the package bottle of those new lots of controls and calibrations.
Mixing skills
After placing the controls and calibrations for 15mins, mix them as followings. Mixing time for
each tube should be NOT less than 5mins with enough time for each step.
1. Tube heads upward. Rub the tube with two hands in low speed, for 1.5~2mins.
Figure 8-1 Tube top upward
8-2
2. Tube heads downward. Rub the tube with two hands in low speed, for 1.5~2mins.
Figure 8-2 Tube top downward

3. Make tube parallel to the ground, and rub the tube with two hands in low speed, for
1.5~2mins.
Figure 8-3 Tube placed horizontally

4. Place the tube in headstand after mixing to check the result. Mix the controls and
calibrations until reaching the following standard.
Good result. There is

Bad result. The
no clot or black
bottom is covered
residue.
by clot and black
residue.
Figure 8-4 Comparison of well mixed sample and poorly mixed sample
5. Cap the tube well after each test of controls or calibrations to ensure their stability.
6. After mixing well, you need only to shake the controls or calibrations slightly before each
test.
7. After a group tests or being placed for more than 5mins, controls or calibrations should be
mixed as well as STEP 4 before they are use again.
8-3
NOTES
1. Put the controls and calibrations back to the refrigerator for more than 1 hour after using.
2. NOT place the controls and calibrations uncapped during the whole process, in case that
the evaporation impacts the result.
3. During the breaks of a group of tests, you need only to shake the controls or calibrations
slightly, in case that severe shaking impacts the result.
4. After opened, the period of validity is 14 days for controls is and 7 days for calibrations in
refrigeration environment. Controls and calibrations out of period of validity are prohibited to
use again.
8-4
9 Error Information
9.1 Error Information and Code
Error Name Error code Error Name Error code

X direction movement error of
0x30000200 Vacuum is out of the range 0x30000603
the sampling assembly
Building pressure is not
Y direction movement error of
0x30000204 allowed in the vacuum 0x30000621
chamber.
Steps error occurs when
sampling assembly adjusts in 0x30000221 WBC clog 0x30000700
the X direction
Steps error occurs when WBC bubbles (liquid
sampling assembly adjusts in 0x30000224 remains in the volumetric 0x30000701
the Y direction tube)
Sampling assembly is working. 0x30000227 RBC clog 0x30000702
RBC bubbles (liquid
Sampling assembly X motor
0x30000228 remains in the volumetric 0x30000703
movement overtime
tube)
Sampling assembly action HGB blank voltage
0x30000229 0x30000720
forbidden abnormal
Sampling assembly Y motor
0x3000022B HGB value abnormal 0x30000721
movement overtime
Sampling assembly action Temperature out of the
0x3000022C 0x30000500
forbidden operating range
Sampling assembly Y motor No rinse (hardware
0x3000022E 0x30000521
piercing error reported)
ASP/LS syringe fails to find the No rinse (reported by
0x30000300 0x3000052A
home position software)
No diluent (reported by
ASP/LS motor is working 0x30000320 0x30000523
software)
No lyse (reported by
ASP/LS motor action failure 1 0x30000321 0x30000525
software)
ASP/LS motor action failure 2 0x30000322 Waste container is full. 0x30000528
Action 1 is not allowed by
0x30000323 Diluent expired 0x30000A01
ASP/LS motor.
Action 2 is not allowed by
0x30000324 Rinse expired 0x30000A02
ASP/LS motor.
ASP/LS motor action overtime 0x30000325 Lyse expired 0x30000A03
9-1
Error Information
DIL syringe fails to find the

0x30000301 Reagent kit expired 0x30000A05
home position.
DIL motor is working. 0x30000326 56V voltage abnormal 0x30000540
12V analogue voltage
DIL motor action failure 1 0x30000327 0x30000541
abnormal
-12V analogue voltage
DIL motor action failure 2 0x30000328 0x30000542
abnormal
Aspiration and draining action 24V power voltage
0x30000329 0x30000543
1 is not allowed by DIL motor. abnormal
Aspiration and draining action 12V power voltage
0x3000032A 0x30000544
2 is not allowed by DIL motor. abnormal
Driver board 5V voltage
DIL motor action overtime 0x3000032B 0x30000545
abnormal
Sample compartment
0x30000400 Printer out of paper 0x30000B00
movement error
Sample compartment
0x30000403 Other errors of the printer 0x30000B02
micro-switch error
Sample compartment is not in
0x30000420 Recorder out of paper 0x30000C00
the close position
Sample compartment motor is
0x30000421 Recorder overheat 0x30000C02
working.
Sample compartment action
0x30000423 Recorder fails to respond. 0x30000C03
overtime
Building pressure error 0x30000600 Data communication error 0x30000D00
Pressure out of range 0x30000602 Front cover is opened. 0x30000580
Building pressure is not
allowed in the pressure 0x30000620 Background abnormal 0x30000A00
chamber.
Building vacuum error 0x30000601
9.2 Error analysis and processing
If Remove Error does not work in the following error mode, check and deal with the errors as
instructed in the following table.
9.2.1 Vacuum and Pressure errors

Error
Causes analysis Troubleshooting
Name
9-2
Error Information
1. The vacuum pump is abnormal, check if the pump is

working when building the pressure and whether there
is air leakage in the pump.
When the time for 2. Check if there is air leakage in the vacuum chamber.
Building building pressure in the 3. Check if there is air leakage in the tubes, valve and
vacuum vacuum chamber is up, volumetric tube connected to the vacuum chamber or
error the final pressure value vacuum pump.
is lower than expected. 4. Check whether the pressure sensor and its tubing
on the volumetric board are normal.
5. If the problem persists, consider to change the
volumetric board or driver board.
1. The vacuum pump is abnormal, check if the pump is
working when building the pressure and whether there
is air leakage in the pump.
2. Check if there is air leakage in the vacuum chamber.
The pressure value in
Vacuum is 3. Check if there is air leakage in the tubing, valve and
the vacuum chamber
out of the volumetric tube connected to the vacuum chamber or
checked is out of the
range vacuum pump.
assigned range.
4. Check whether the pressure sensor and its tubing
Building
pressure is
Instruction collision
not allowed
when building pressure Change driver board or data board.
in the
in the vacuum chamber
vacuum
chamber.
1. The pressure pump is abnormal, check if the pump
is working when building the pressure and whether air
leakage exists in the pump and pressure filter.
When the time for 2. Check if there is air leakage in the pressure
Building building pressure in the chamber.
pressure pressure chamber is up, 3. Check if there is air leakage in the tubing and valve
error the final pressure value connected to the pressure chamber or pressure pump.
is lower than expected. 4. Check whether the pressure sensor and its tubing
1. The pressure pump is abnormal, check if the pump
The pressure value in
is working when building the pressure and whether air
Pressure the pressure chamber
leakage exists in the pump and pressure filter.
out of range checked is out of the
2. Check if there is air leakage in the pressure
assigned range.
chamber.
9-3
Error Information
3. Check if there is air leakage in the tubing and valve

connected to the pressure chamber or pressure pump.
4. Check whether the pressure sensor and its tubing
Building
pressure is
Instruction collision
not allowed
when building pressure Change driver board or data board.
in the
in the pressure chamber
pressure
chamber.
9.2.2 Reagent errors

Causes
Error Name Troubleshooting
analysis
1. Check if there are reagents in the rinse
reagent bottle.
2. If there is enough amount in the reagent
bottle, check the bubble number in the rinse
The tubing tubing. If there are many bubbles, check if there
No rinse (hardware status checked is air leakage in the rinse tubing.
reported) in assigned time 3. If there is no bubble in the tubing, then
is empty. calibrate the rinse photocoupler again; see
section 2.4.2 for the methods.
4. If the problem persists after the calibration of
the rinse photocoupler, check the photocoupler
or driver board and change the ineffective parts.
The residual
No rinse (reported by Change for new reagents or change the residual
amount is lower
software) amount of the reagent.
than 5%.
The residual
No diluent (reported by Change for new reagents or change the residual
amount is lower
than 5%.
The residual
No lyse (reported by Change for new reagents or change the residual
amount is lower
than 5%.
If the error is still reported when the waste
container is not full:
The dobber has
Waste container is full. 1. Check if the waste sensor inlet is connected
already floated.
reliably to the BNC connector at the back of the
analyzer.
9-4
Error Information
2. Take the dobber out of the waste container to

see if the dobber drops reliably.
Expiration date
Change for new reagent or change the
Diluent expired is out of the set
expiration date.
range.
Expiration date
Rinse expired is out of the set
expiration date.
range.
Expiration date
Lyse expired is out of the set
expiration date.
range.
Expiration date
Reagent kit expired is out of the set
expiration date.
range.
9.2.3 Hardware errors

Error Name Causes analysis Troubleshooting
X direction motor
X direction
photocoupler error, or the 1. Check whether the photocoupler is normal.
movement error
motor fails to run to the 2. Check if the motor and drive assembly miss
of the sampling
photocoupler due to missing the steps or are resisted.
assembly
steps or being resisted.
Y direction motor
Y direction
photocoupler error, or the 1. Check whether the photocoupler is normal.
movement error
motor fails to run to the 2. Check if the motor and drive assembly miss
of the sampling
photocoupler due to missing the steps or are resisted.
assembly
steps or being resisted.
Steps error
occurs when
sampling The adjusted steps in X
Adjust again
assembly direction exceed the limit.
adjusts in the X
direction
Steps error
occurs when
sampling The adjusted steps in Y
Adjust again
assembly direction exceed the limit.
adjusts in the Y
direction
Sampling
Control instruction collision of
assembly is Change driver board or data board.
working.
9-5
Error Information
Sampling
assembly X
X motor actual action
motor Change driver board or data board.
overtime
movement
overtime
Sampling
When the sampling assembly 1. Check whether the photocoupler is normal.
assembly
is working, the X motor is not 2. Check if the motor and drive assembly miss
action
in the allowed position. the steps or are resisted.
forbidden
Sampling
assembly Y
Y motor actual movement
motor Change driver board or data board.
overtime
movement
overtime
Sampling
When the sampling assembly 1. Check whether the photocoupler is normal.
assembly
is working, the Y motor is not 2. Check if the motor and drive assembly miss
action
in the allowed position. steps or are resisted.
forbidden
Sampling When Y motor is piercing, the
1. Check whether the photocoupler is normal.
assembly Y baffle fails to pass the piercing
2. Check the piercing action of the sample
motor piercing center of the photocoupler
probe.
error area.
ASP motor photocoupler
error, or the motor fails to run
ASP/LS syringe 1. Check whether the photocoupler is normal.
to the photocoupler position
fails to find the 2. Check if the motor and drive assembly miss
for missing steps or the motor
home position. steps or are resisted.
cannot drive the sample
probe to pierce the cap.
ASP/LS motor Control instruction collision of
Change driver board or data board.
is working. the ASP motor
After the movement: The
moving steps of the ASP
ASP/LS motor motor displayed that it shall
2. Check if the motor and drive assembly miss
action failure 1 be in the photocoupler area,
steps or are resisted.
and the photocoupler is not
blocked.
ASP/LS motor motor displayed that it shall
2. Check if the motor and drive assembly miss
action failure 2 not be in the photocoupler
steps or are resisted.
area, but the photocoupler is
blocked.
Action 1 is not Before the movement: The 1. Check whether the photocoupler is normal.
allowed by moving steps of the ASP 2. Check if the motor and drive assembly miss
9-6
Error Information
ASP/LS motor. motor displayed that it shall steps or are resisted.

be in the photocoupler area,
and the photocoupler is not
blocked.
Before the movement: The
Action 2 is not 1. Check whether the photocoupler is normal.
motor displayed that it shall
allowed by 2. Check if the motor and drive assembly miss
not be in the photocoupler
ASP/LS motor. steps or are resisted.
area, but the photocoupler is
blocked.
ASP/LS motor
ASP motor actual movement
movement Change driver board or data board.
overtime
overtime
DIL motor photocoupler error,
or the motor fails to run to the
DIL syringe fails 1. Check whether the photocoupler is normal.
photocoupler position for
to find the 2. Check if the motor and drive assembly miss
missing steps or the motor
home position. steps or are resisted.
cannot drive the sample
probe to pierce the cap.
DIL motor is Control instruction collision of
working. the DIL motor
moving steps of the DIL motor 1. Check whether the photocoupler is normal.
DIL motor
displayed that it shall be in the 2. Check if the motor and drive assembly miss
action failure 1
photocoupler area, and the steps or are resisted.
photocoupler is not blocked.
DIL motor
displayed that it shall not be in 2. Check if the motor and drive assembly miss
action failure 2
the photocoupler area, but the steps or are resisted.
photocoupler is blocked.
Aspiration and
draining action
displayed that it shall be in the 2. Check if the motor and drive assembly miss
1 is not allowed
photocoupler area, and the steps or are resisted.
by DIL motor.
photocoupler is not blocked.
Aspiration and
draining action
displayed that it shall not be in 2. Check if the motor and drive assembly miss
2 is not allowed
the photocoupler area, but the steps or are resisted.
by DIL motor.
photocoupler is blocked.
DIL motor
X motor actual movement
movement Change driver board or data board.
overtime
overtime
9-7
Error Information
Photocoupler error of the

close position of the sample
Sample 1. Check whether the photocoupler is normal.
compartment, or the sample
compartment 2. Check if the motor and drive assembly miss
compartment misses steps or
movement error steps or are resisted.
fails drive the sample probe to
pierce the cap.
Sample Micro-switch error or the
1. Check if the adapter is placed in the correct
compartment sample compartment closes
position.
micro-switch when the adapter is not
2. Check whether the micro-switch is normal.
error placed.
When the sampling assembly
Sample 1. Check whether the photocoupler is normal.
is moving downward at the
compartment is 2. Check whether the micro-switch is normal.
aspirating position, the
not in the close 3. Check whether the sample compartment is
sample compartment does
position pressed and closed reliably when closes.
not close.
Sample
Control instruction collision of
compartment
the sample compartment Change driver board or data board.
motor is
motor
working.
Sample
compartment Sample compartment motor
movement actual movement overtime
overtime
Recorder out of paper or the
Recorder out of
recorder paper is not Install new recorder paper.
paper
positioned in the right place
Continuous printing time is too long; the
Recorder Recorder thermal head
problem is removed when it is cooled down
overheat overheat
and prints again.
Abnormal recorder driver
Recorder fails Check whether the recorder and cables are
board, thermal head or cables
to respond. normal.
and so on
The front cover is opened or 1. Check if the front cover is closed.
Front cover is the connecting line of the front 2. If the problem persists when the front cover
opened. cover detecting sensor is is closed, then check whether the connecting
damaged. line of the sensor is reliable.
9.2.4 Voltage errors

Error
Causes analysis Troubleshooting
Name
1. Check whether the 56V test point on the data board
56V voltage The voltage detected is
is normal.
abnormal out of range
2. Check whether the analogue +12V test point on the
9-8
Error Information
data board is normal.

3. Remove J4 from the data board; check whether the
analogue 12V voltage from the power supply is
normal.
4. Change the data board or power supply board
based on the checking results.
1. Check whether the analogue +12V test point on the
12V
analogue The voltage detected is
analogue +12V voltage from the power supply is
voltage out of range
normal.
abnormal
1. Check whether the analogue -12V test point on the
-12V
analogue The voltage detected is
analogue -12V voltage from the power supply is
voltage out of range
normal.
abnormal
1. Check the whether 24V test point on the driver
board is normal.
24V power
The voltage detected is 2. Remove J5 from the data board; check whether the
voltage
out of range 24V voltage from the power supply is normal.
abnormal
3. Change the driver board or power supply board
1. Check whether the 12V test point on the driver
board is normal.
12V power
voltage
abnormal
1. Check whether the 5V test point on the driver board
is normal.
Driver board
5V voltage
abnormal
9.2.5 Measurement errors

9-9
Error Information
1. Check whether the reagents are connected

correctly. Especially note that whether the waste is
connected to the reagent inlet.
2. Perform background counting after all the reagents
are changed.
3. If the problem persists, open the front cover and
Background Background out of shielding box, and then perform background counting.
abnormal range Observe whether the fluid dispensing of WBC and
RBC bath are normal, if the charging tube or sample
probe build many small bubbles into the bath, then
check the error of the tubing and the sample
probe.(Note: Before the photocoupler of the volumetric
tube is triggered, close the cover of the shielding box).
4. Check the shielding structure of the bath.
1. Ensure the HGB assembly lighting source lightens,
the fluidic level covers the HGB light, and then adjust
the gain in the gain adjusting screen, the HGB blank
HGB blank HGB blank voltage voltage is 4.50.1V.
voltage not within the range 2. Check whether the fluid dispensing of WBC bath is
abnormal of [3.2V,4.9V] normal; ensure that there are no abnormal conditions
such as insufficient fluids and building bubbles.
3. If the problem persists after the above checking,
then change the HGB assembly.
1. Ensure the HGB assembly lighting source lightens,
the fluidic level covers the HGB light, and then adjust
the gain in the gain adjusting screen, the HGB blank
voltage is 4.50.1V.
HGB value The output HGB
2. Check whether the fluid dispensing of WBC bath is
abnormal value is lower than 0.
normal; ensure that there are no abnormal conditions
such as insufficient fluids and building bubbles.
3. If the problem persists after the above checking,
then change the HGB assembly.
1. Enter the maintenance screen, zap and flush for
more than 3 times.
The triggering time
2. If the problem persists, then soak with the probe
of the first WBC
cleanser.
WBC clog photocoupler or the
3. If the problem persists, check if there is air leakage
count time is too
in the tubing from the back bath to the volumetric tube.
long
4. If the problem persists, then remove the aperture;
check whether the aperture is clogged.
WBC bubbles The triggering time 1. Check whether liquid remains in the volumetric tube.
(liquid remains of the first WBC 2. Check whether there is air leakage in the volumetric
in the photocoupler or the tube and the tee connector which enable the bubbles
volumetric count time is too to get into the volumetric tube.
9-10
Error Information
tube) short.
The triggering time

of the first RBC
RBC clog photocoupler or the Use the same troubleshooting method as WBC clog
count time is too
long.
RBC bubbles The triggering time
(liquid remains of the first RBC
Use the same troubleshooting method as WBC
in the photocoupler or the
bubbles
volumetric count time is too
tube) short.
9.2.6 External connection errors

Place in the printing paper again, trigger the printer to

Printer out of paper or
Printer out of go on printing (Different operations are used by
improper printing paper
paper different models of the printers, see the printer
placing
operator's manual for details).
Check whether the status of the printer is normal and
Other errors Status of the printer is
remove relevant errors. (see the printer operator's
of the printer abnormal
manual for details)
9.2.7 Temperature errors

1. Check whether the temperature shown by the
The temperature
Temperature device matches the real temperature.
detected by the
out of 2. Check whether the cables and connectors of the
temperature sensor is
operating temperature sensor are normal.
out of the operating
range 3. If the problem persists, then change the driver
range.
board.
9.2.8 Software errors
1. Check whether correct cable connections

Data Abnormal connection (network cable/serial port wire) are used between
communication or incorrect the LIS computer and the main unit.
error communication setting 2. Check whether the LIS software is corresponding
to the communication setting of the main unit.
9-11
Error Information
9.3 Error diagnosis assisted by abnormal histogram
Note: The following figures are got from the R & D debug platform and are only used to assist
the error diagnosis with the help of the histograms.
9.3.1 WBC histogram diagnosis

Comparison of normal and abnormal WBC histogram:
WBC histogram of normal sample is as shown in Figure 9- 1, the three-part differentiate is
clear, and the WBC value is 7.1.
Figure 9-1 Normal WBC histogram
If the lyse reaction is abnormal in the WBC channel, the shape of the WBC histogram varies as
shown in the following Figure 9-2.
Figure 9-2 WBC histogram of abnormal blood
Possible causes analysis:

The error shown in Figure 9-2 occurs because WBC fails to generate bubbles in the lyse
reaction phase. The WBC fails to react fully because of no generation of bubbles, and creates
main peak in the 50~100fL channel, the WBC particles which are not fully reacted and part of
the RBCs which are not sufficiently lysed. The statistics shows that the WBC value is 34.6.
Key point of maintenance and repair:
9-12
Error Information
Observe the variation of the WBC histogram. If the peak of the histogram moves slightly to the
right, generally it is because of insufficient reaction, check whether the adding of reagents in
WBC bath is normal, or change for another batch of reagents. If the WBC histogram varies
seriouslyit is possibly caused by insufficient lyse, check the adding of reagents, and also
relevant factors such as the mixing up the WBC bath, liquid level of the front bath, bubbles in
the back bath and so on.
9.3.2 RBC/PLT histogram diagnosis

Comparison of normal and abnormal RBC/PLT histogram:
Figure 9-3 Normal RBC/PLT histogram
Figure 9-4 Abnormal RBC/PLT histogram
Possible causes analysis:

If there are many bubbles in the RBC back bath, the histogram of abnormal data may be got as
shown. The bubbles of back bath may be caused by the following reasons:
1. There is insufficient fluid in the RBC bath, when the samples flow by the aperture, the
fluid level is lower than the aperture, so that the RBC back bath aspirates the bubbles
from the front bath.
2. There is air leakage in the back bath; bubbles are aspirated through the gap of the
assembly when counting.
3. The back bath is not cleaned thoroughly because of the valve error; the bubbles
generated by zapping gather in the back bath and fail to be flushed away.
Meanwhile, the pulse signals are totally irregular and the baseline disappears, the RBC
histogram is totally abnormal, the main PLT area of PLT histogram uplifts.
Key point of maintenance and repair:
1. Observe the fluid level of the front bath carefully.
2. Observe whether the bubbles are flushed away when cleaning the back bath.
9-13
Error Information
9.3.3 Histogram examples of typical samples

The numbers in the following histogram indicate relevant position of the discriminator. The
numbers do not carry the unit fL; the histograms are only used for reference.
1. Normal sample
Parameter result Histogram
Figure 9-5 Histogram of normal sample
2. Iron deficiency anemia

Figure 9-6 Histogram of iron deficiency anemia sample
9-14
Error Information
3. Hemolytic anemia
Figure 9-7 Histogram of hemolytic anemia sample
4. Acute lymphoblastic leukemia

Figure 9-8 Histogram of acute lymphoblastic leukemia sample
9-15
Error Information
5. Acute promyelocytic leukemia (M3, granulocyte polymorpho nuclear which

causes the histogram of the granulocytes shifts to the left)
R3
Figure 9-9 Histogram of acute promyelocytic leukemia sample
6. Acute monocytic leukemia (M5b)

R2R3Rm
Figure 9-10 Histogram of acute monocytic leukemia sample
9-16
Error Information
7. Chronic myelocytic leukemia, (CML)

R2R3R4Rm
Figure 9-11 Histogram of chronic myelocytic leukemia sample
8. Acute megakaryocytic leukemia (M7)

R2R3Rm
Figure 9-12 Histogram of acute megakaryocytic leukemia sample
9-17
Error Information
9. During the treatment of chronic myelocytic leukemia--interference of nucleated

red blood cells (NRBCs)
R1R3Rm
Pm
Figure 9-13 Histogram of the chronic myelocytic leukemia sample during the treatment with
interference of NRBC
10. Reason for leukopenia-inteference of RBC debris

Pm
Pl
Figure 9-14 Reason for leucopenia - Histogram with interference of RBC debris
9-18
Error Information
11. Thrombocytosis
Figure 9-15 Histogram of thrombocytosis
12. Acute myeloblastic leukemia -partial maturity (transferred from MDS)

R1R2R3Rm
Figure 9-16 Histogram of sample of acute myeloblastic leukemia-partial maturity
9-19
Error Information
13. Neutrophil granulocyte increases

Figure 9-17 Histogram of neutrophil granulocyte increase sample
14. Lymphocytosis
R3
Pm
Figure 9-18Histogram of lymphocytosis sample
9-20
Error Information
15. Monocytosis
R2R3Rm
Figure 9-19 Histogram of monocytosis sample
9.4 Error diagnosis assisted by abnormal pulse figure
The browse function of the present sample pulse of the system helps the analyzer a lot to
diagnose the complicated errors.
9-21
Error Information
9.4.1 Pulse of background count
Figure 9-20 WBC pulse of background count
Figure 9-21 RBC pulse of background count
9-22
Error Information
Figure 9-22 PLT pulse of background count
When the background is clean, most part of the channels of background count is saturated
pulse formed by the small bubbles as shown in the above figure. Only a small quantity of
particles scatter everywhere in the pulse figure.
9.4.2 Pulse of normal sample
Figure 9-23 WBC pulse of normal sample
9-23
Error Information
Figure 9-24 RBC pulse of normal sample
Figure 9-25 RBC pulse of normal sample
In normal condition, the pulse width of WBC, RBC and PLT are generally consistent, but the
pulse peak of WBC is distributed unevenly, while the pulse heights of RBC are distributed
consistently, and the pulse peak of PLT is the lowest. When refer to the pulse spacing the
pulse distribution of WBC are sparse, while the RBC distribution is dense, the PLT is generally
scattered between other pulses.
9-24
Error Information
9.4.3 Interference pulse
Figure 9-26 WBC interference of background count
Figure 9-27 RBC interference of background count
Figure 9-28 WBC interference of background count
The above figure shows the interference pulses of each channel when the shielding of the bath
is ineffective. Feature: The shapes of the pulses are irregular, the pulses overlap a lot, and the
pulse width is narrow, while the heights are distributed unevenly.
9-25
Error Information
9.4.4 Pulse of back bath of no fluid
Figure 9-29 RBC pulse when there is no fluid in the back bath
Figure 9-30 PLT pulse when there is no fluid in the back bath
When there is no fluid at the back bath or when a lot of bubbles are aspirated, the RBC/PLT
signal baseline will disappear; in the meantime, the pulse will be fully saturated.
9-26
10 Appendices
A List of Spare Parts
Please provide the code and name of the MINDRAY spare parts listed in this manual when you
order and purchase spare parts from MINDRAY. This will help us offer you the spare parts you
need as soon as possible. Choosing spare parts by MINDRAY will ensure the perfect working
state of you analyzer.
A.1 List of Spare PCBA
No. Description MINDRAY Remark
code/model
1 Power supply board 801-3007-00001-00
2 Data board 801-3007-00002-00
3 Driver board 801-3007-00003-00
4 Touchscreen control board 801-3007-00004-00
5 Indicator board 801-3007-00005-00
6 Recorder driver board 801-3007-00006-00
7 USB interface board 801-3007-00007-00
8 Volumetric board 801-3007-00008-00
9 Sample compartment connecting only applicable to
801-3007-00009-00
board closed tube model
A.2 List of Spare parts

Description MINDRAY Remark
No.
code/model
1 Touchscreen 801-3900-00037-00
2 CF card with software 801-3007-00010-00
3 Screen assembly 801-3007-00011-00
4 Recorder assembly 801-3007-00012-00
5 Power supply assembly 801-3007-00013-00
6 HGB assembly 801-3900-00041-00
801-3900-00061-00
7
Front cover door detect assembly
8 Photocoupler and connecting cable 801-3900-00039-00
Liquid-level photocoupler and
9
connecting cable 801-3900-00040-00
10 Sensor assembly of the syringe 801-3900-00059-00
A-1
Appendices
motor
11 Micro-switch 801-3007-00014-00
12 WBC bath assembly 801-3900-00001-00
13 RBC bath assembly 801-3007-00015-00
WBC bath (without aperture)
14
maintenance and repair part 801-3900-00003-00
RBC bath (without aperture)
15
WBC bath (without HGB unit)
16
Maintenance and repair part of bath
17
washer 801-3900-00006-00
18 Tube kit under the bath 801-3007-00016-00
19 Aperture sensor (D80um) 801-2800-00013-00
20 RBC aperture (D70um) 801-3110-00078-00
21 HGB bath O-ring 801-3900-00095-00
22 Sampling assembly 801-3007-00017-00
23 Vertical action assembly 801-3007-00018-00
24 Horizontal action assembly 801-3007-00019-00
only applicable to
25
Probe wipe clamp (Open vial) 801-3900-00013-00 open vial model
only applicable to
26
Probe wipe clamp (Closed tube) 801-3900-00014-00 closed tube model
only applicable to
27
Closed-tube sample probe 801-3900-00015-00 open vial model
only applicable to
28
Open-vial sample probe 801-3101-00002-00 closed tube model
29 10ml syringe assembly 801-3900-00054-00
30 Single syringe drive assembly 801-3900-00053-00
31 10ml syringe 801-3900-00009-00
32 Double syringe assembly 801-3900-00051-00
33 Double syringe drive assembly 801-3900-00052-00
34 2.5ml syringe 801-3900-00007-00
100ul syringe maintenance and
35
repair part 801-3900-00008-00
Sample compartment assembly only applicable to
36 801-3007-00020-00
closed tube model
Sample compartment door for sales only applicable to
37 801-3007-00021-00
closed tube model
Sample compartment door for only applicable to
38 801-3007-00022-00
bidding closed tube model
only applicable to
39 801-3007-00023-00
Predilute adapter closed tube model
A-2
Appendices
only applicable to
40 Adapter assembly 1 801-3007-00024-00
closed tube model
only applicable to
41 Adapter assembly2 801-3007-00025-00
closed tube model
42 Probe wipe fixing pin 801-3900-00096-00
only applicable to
43 801-3007-00026-00
Aspirate key for sales open vial model
only applicable to
44 801-3007-00027-00
Aspirate key for bidding open vial model
only applicable to
45
Tube barrier 801-3900-00094-00 closed tube model
46 Sample holder tighten assembly 801-3900-00046-00
47 Rinse cap assembly 801-3007-00028-00
48 Lyse cap assembly 801-3007-00029-00
49 Diluent cap assembly 801-2800-00018-00
Waste cap assembly (drain waste to
50
waste container) 801-3900-00020-00
51 Tube bracket assembly 801-3007-00030-00
52 Fluidic pump assembly 801-3900-00022-00
53 Gas pump assembly 801-3007-00031-00
54 Shielding box assembly 801-3007-00032-00
Maintenance and repair par of
55
volumetric tube 801-3101-00047-00
56 Waste pump filter assembly 801-3900-00024-00
57 Filter of the isolation chamber 801-3900-00010-00
58 Air filter 801-1805-00026-00
59 Pressure chamber assembly 801-1805-00006-00
60 Vacuum chamber assembly 801-1805-00005-00
Customized mini tee electromagnetic
61
valve maintenance package 801-0030-00001-00
Customized Two-way mini
62 electromagnetic valve maintenance
package 801-0030-00002-00
63 Back board connector package 801-3007-00033-00
64 Tubing package 801-3900-00083-00
65 Thick 50 tubing package 801-3900-00097-00
66 Thin 50 tubing package 801-3900-00098-00
67 1.5 TEFLON tube package 801-3900-00099-00
68 3603 thick tube package 801-3900-00100-00
69 MPF tube package 801-3900-00101-00
70 Middle silica gel tube package 801-3900-00102-00
Middle silica gel transferring tube
71 801-3900-00103-00
package
A-3
Appendices

Small silica gel transferring tube
73 801-3900-00105-00
package
75 Sampling tube package 801-3900-00107-00
76 Thick EVA tube package 801-3900-00108-00
77 Black transferring tube package 801-3900-00109-00
78 Thick TPU tube package 801-3900-00110-00
79 Cable package 801-3007-00034-00
80 Screw package 801-3007-00035-00
81 Connector package 801-3900-00092-00
82 Tools kit 801-3007-00036-00
83 On-site Maintenance Package 801-3007-00037-00
only applicable to
84 801-3007-00038-00
BC-3600 open vial cover open vial model
only applicable to
85 801-3007-00039-00
BC-3600 closed-tube cover closed tube model
only applicable to
86 801-3007-00040-00
BC-3300 open vial cover open vial model
only applicable to
87 801-3007-00043-00
BC-3600 closed-tube cover closed tube model
A.3 Cable list

No. Description MINDRAY Bidding no. Remark
code/model corresponding to
appendix C
1 D5V/P12V/P24V power cord 009-001367-00 (1)
2 Zap power cord 009-001366-00 (2)
3 Data board A12V power cord 009-001368-00 (3)
Communication cable of the
(4)
4 volumetric board 3900-20-85357
Connecting wire harness of
5 the motor control (open vial) 009-001363-00 (5)
the motor control (closed (5)
6 tube) 009-001417-00
7 Safe ground wire of syringe 009-001195-00 (6)
(7)
8 valve control 009-001376-00
Pump control Connecting
(8)
9 wire 009-001373-00
Connecting wire of
(9)
10 temperature sensor 3900-20-85347
A-4
Appendices

photocoupler signal (closed
11 tube) 009-001365-00 (10)
(11)
12 photocoupler 009-001412-00
Sample compartment
(12)
13 micro-switch transferring wire 009-001375-00
Connecting wire of data
(13)
14 board and power driver board 3900-20-85342
15 USB Connecting wire 009-001419-00 (14)
16 RBC signal wire 3900-20-85345 (15)
17 WBC signal wire 3900-20-85344 (16)
18 HGB signal wire 3900-20-85343 (17)
19 Connecting wire of recorder 3900-20-85341 (18)
20 Ground wire of recorder 009-001364-00 (19)
Signal detecting wire of door
(20)
21 opening 3900-20-85379
Connecting wire of LCD
(21)
22 screen signal 009-001418-00
Signal wire of front cover
(22)
23 assembly 009-001369-00
Ground wire of the screen
(23)
24 backboard 009-001374-00
Transferring wire of LCD
(24)
25 screen backlight board 2800-20-28768
Photocoupler and Connecting
(25)
26 wire (rinse detecting) 3900-21-85368
WIRE three-core power cord
/
27 of national standards 509B-10-05996
A.4 Consumable list

No. Description MINDRAY
code/model Remark
1. Thermal print paper of the recorder A30-000001---
2. Tubing package 801-3900-00083-00
3. only applicable to
Centrifugal tube (1.5ml) A21-000013--- closed tube model
4. Cellular sampling cup A22-000005---
5. Plastic test tube 12*100 (5ml) A21-000012---
6. Mindray B30 controls P (high 0031-30-84473
level/3ml)
7. Mindray B30 controls P (normal 0031-30-84476
level/3ml)
A-5
Appendices
8. Mindray B30 controls P (low 0031-30-84479

level/3ml)
9. Mindray S30 calibrators (3ml) 0031-30-84549
10. Mindray M-30PD diluent (20L) A12-000099---
11. Mindray M-30PCFL cyanide-free
lyse (150ml) A11-000079---
12. Mindray M-30PCFL cyanide-free
lyse (250ml) A12-000103---
13. Mindray M-30PCFLcyanide-free
lyse (500ml) A12-000104---
14. Mindray rinse (5.5L*2) A12-000107---
15. Mindray probe cleanser (50ml) 105-000385-00
A-6
Appendices
B List of Damageable and Consumable Parts
No. Material name MINDRAY code/model Remark

1 only applicable to
Probe wipe clamp (open vial) 801-3900-00013-00 open vial model
2 Probe wipe clamp (closed only applicable to
tube) 801-3900-00014-00 closed tube model
3 only applicable to
Closed-tube sample probe 801-3900-00015-00 closed tube model
4 Air filter 801-1805-00026-00
B-1
Appendices
C Electricity Connection Diagram of the Analyzer
Description of the electricity connection diagram of the analyzer

1) The dashes marked with in the following diagram only indicates the connection of closed
tube model, the real line marked with indicates that the connections of the open vial model
and closed tube model are the same;
2) The dashes marked with in the following diagram only indicates the connection of close
tube model, there is no such connection for the open vial model;
12
3) The dashes marked with in the following diagram indicates the connection of closed
tube model;
4) The rest of the connections are the same for closed tube model and open vial model;
5) See Appendix A.3 for the details of the list of cable codes.
6) The electricity connection diagram of the analyzer is shown as follows.
C-1
Appendices
C-2
Appendices
D Fluidic Diagram
D-1
Appendices
E Pump and Valve Function Table
E.1 Fluidic Valve Function Table

Description Function 2-way/3-way
Switching the aspirating and draining operations of the diluent
V01 3
syringe
Providing diluent to probe wipe, connecting the diluent syringe with
V02 3
the probe wipe inlet when V02 is open
V03 Switching between aspiration and draining of the lyse 3
Providing diluent to sample probe, connecting the diluent syringe
V04 3
with the sample probe when the V04 is open
V05 Draining the WBC volumetric tubes 2
V06 Draining the RBC volumetric tubes 2
V07 Use for the control of WBC count 2
V08 Use for the control of RBC count 2
Control the connection between the vacuum chamber and pressure
V09 2
chamber
Control the connection between the pressure chamber and the
V10 2
isolation chamber of WBC bath, generate bubbles in the WBC bath
Control the connection between the pressure chamber and the
V11 2
isolation chamber of RBC bath, generate bubbles in the RBC bath
Adding diluent into the RBC bath, connecting the diluent syringe
V13 3
passage to the fluidic adding tube of RBC bath
V14 Control the washing of the WBC back bath together with V07 2
V15 Control the washing of the RBC back bath together with V08 2
Control the connection between isolation chamber and the waste
V16 2
pump, and drain the WBC bath
Control the connection between isolation chamber and the waste
V17 2
pump, and drain the RBC bath
Control the connection between vacuum chamber and the waste
V18 2
pump, use for building vacuum and draining the waste
V19 Control the draining of probe wipe waste 2
E.2 Pump Function Table

Description Function
Gas pump Building pressure in the pressure chamber, generating bubbles and flushing
(GP) the aperture with the pressure
Waste pump Draining the vacuum chamber and building vacuum in the vacuum chamber;
(LP) Draining the waste in the WBC bath, RBC bath and the probe wipe.
E-1
Appendices
E.3 Syringe Function Table

Description Function
Sampling syringe (100ul) Aspirating and dispensing sample
Lyse syringe (2.5ml) Adding lyse to WBC bath
Adding diluents to the WBC bath and the RBC bath, cleaning the
Diluent syringe (10ml)
sample probe and adding diluents to the probe wipe
E-2
Appendices
F Tubing
F.1 Fluidic Disposition Chart
C21 DILUNET
T47 T48
T2
RINSE
J5-T20-
J6
SV2 T49
SV1 SV13 J3-T56-
T3
J4
SV4 SV7
SV5
J9-T64-
SV3
T34 J10
C26 GF3
C4
C16 T31 T15 T14
T35 T53 Rinse
GF4
container
SV6
T32
GF1
SV8
C3
C13 C15 T13 T12
T30
T33 T39
GF2
Volumetric
T54 Lyse
tube 1
Rinse bottl
Pressure detectin e
Pressure sensor C22 C24 g
sensor T85 T81 sensor
T50
T40 T87 T83
SPB T68 T28 RBC WBC
T51 bath bath
T79 C27 T88 T82T84
T52
PC Probe wipe T97 C1 C2
T80 VC C23 T67 C25 T66
C14 SV14
LF
SV15 T37
T36 C20
SV9
T98 T86
T38
T6 T11 J18-T62-
GP T18 J20 SV10
SV11
T10 J1
C9 C8 J19 J21J22-T63-J24J23
7
Isolation Isolation
T41 C18 T7 C2 C11
chamber 1
C10 chamber 2
8 T77 T78
C17 T8
T42 T43
C19 T99
GF5 GF6 T9
T69 T70
Waste
interface LP SV18 SV19 SV17 SV16
T29
T29 T27 T26 T22
T24 T21
C7 C6 C5
T25 T23
Waste tank
F-1
Appendices
F.2 Tubing Model

MINDRAY Position in the
No. Name Length (mm)
code/model fluidic chart
1 T1 M6G-020005--- A1 55
2 T2 M6G-020005--- A1 60
3 T3 M6G-020005--- A2 30
4 T4 M6G-020005--- B2 15
5 T5 M6G-020005--- B5 35
6 T6 M90-100071--- C1 160
7 T7 M90-100071--- C2 15
8 T8 M90-100071--- C1 50
9 T9 M90-100071--- C3 75
10 T10 M90-100071--- C1 15
11 T11 M90-100071--- C2 80
12 T12 3001-10-07069 B3 51
13 T13 3001-10-07069 B3 43
14 T14 3001-10-07069 B3 41
15 T15 3001-10-07069 B3 51
16 T16 M90-000025--- B3 38
17 T17 M90-000025--- C3 35
18 T18 M90-100071--- C3 45
19 T20 M90-100031--- A2 430
20 T21 M90-100071--- D4 120
21 T22 M90-100071--- D3 30
22 T23 M90-000025--- D3 40
23 T24 M90-100071--- D2 30
24 T25 M90-000025--- D2 40
25 T26 M90-100071--- D2 30
26 T27 M90-000025--- D2 160
27 T28 A21-000007--- C3 50
28 T29 M90-000025--- D1 90
29 T30 M90-100071--- B2 20
30 T31 M90-100071--- B2 20
31 T32 M90-100071--- B2 15
32 T33 M90-100071--- B2 15
33 T34 M90-100071--- B2 15
34 T35 M90-100071--- B2 15
35 T36 A21-000007--- C1 35
36 T37 M90-100071--- C5 20
37 T38 M90-100071--- C4 90
38 T39 M90-100071--- B5 300
39 T40 A21-000007--- C1 50
40 T41 A21-000007--- C1 30
F-2
Appendices
41 T42 M90-100071--- C1 15
42 T43 M90-100071--- C1 15
43 T47 M90-100071--- A1 70
44 T48 M90-000025--- A4 280
45 T49 3001-10-07069 A5 400
46 T50 A21-000007--- C1 60
47 T51 A21-000007--- C1 30
48 T52 M90-100071--- C1 80
49 T53 3001-10-07069 B4 460
50 T54 3001-10-07069 B3 480
51 T55 M90-100031--- B2 950
52 T56 M90-100031--- A5 610
53 T57 M90-000025--- C6 300
54 T58 A21-000002--- B5 300
55 T60 A21-000002--- B5 180
56 T61 3001-10-07069 B1 830
57 T62 M90-000026 C3 500
58 T63 M90-000026 C4 500
59 T64 M90-100031--- A4 75
60 T65 M90-100031--- B4 80
61 T66 M90-100071--- C4 210
62 T67 M90-100071--- C3 240
63 T68 M90-100071--- C2 660
64 T69 M90-100071--- D3 160
65 T70 M90-100071--- D4 160
66 T72 M6G-020007--- B2 500
67 T73 0040-10-32301 B4 60
68 T74 M90-100031--- B4 60
69 T75 M90-100031--- B4 105
70 T76 M6G-020005--- A2 25
71 T77 M90-100071--- C4 110
72 T78 M90-100071--- C4 50
73 T79 M90-100071--- C1 200
74 T80 M90-100071--- C3 210
75 T81 3001-10-07069 C4 25
76 T82 3001-10-07069 C4 25
77 T83 M90-100071--- C4 15
78 T84 M90-100071--- C4 15
79 T85 3001-10-07069 C3 25
80 T86 3001-10-07069 C3 25
81 T87 M90-100071--- C3 15
82 T88 M90-100071--- C3 15
83 T89 M90-100031--- C4 120
F-3
Appendices
84 T90 M90-100031--- C4 120

85 T92 M6G-020054--- B6 1500
86 T93 M6G-020054--- A5 1000
87 T95 M6G-020055--- D1 1500
88 T97 M90-000025--- C2 70
89 T98 M90-000025--- C2 85
90 T99 M90-100071--- C2 120
F.3 Connector Models

MINDRAY Position in the
No. Name Amount
code/model fluidic chart
1 C1 M90-100028--- C3 1
2 C2 M90-100028--- C4 1
3 C3 M90-100028--- B3 1
4 C4 M90-100028--- B3 1
5 C5 M90-100065--- D3 1
6 C6 M90-100065--- D2 1
7 C7 M90-100065--- D2 1
8 C8 M90-100028-01 C3 1
9 C9 M90-100028-01 C3 1
10 C10 M90-100028-01 C4 1
11 C11 M90-100028-01 C4 1
12 C13 M90-100027-00 B2 1
13 C14 3001-10-07066 C1 1
14 C15 M90-100028-03 B2 1
15 C16 M90-100028-03 B2 1
16 C17 M90-100028-03 C1 1
17 C18 M90-100028-03 C1 1
18 C19 M90-100028-03 C1 1
19 C20 M90-100028-03 C5 1
20 C21 M90-100027--- A2 1
21 C22 M90-100100--- C3 1
22 C23 M90-100100--- C3 1
23 C24 M90-100100--- C4 1
24 C25 M90-100100--- C4 1
25 C26 M90-100100--- B2 1
26 C27 M90-100027--- C2 1
27 C28 M90-100027--- C2 1
F-4
Appendices
F.4 Transferring Tube Models

No. Name MINDRAY code/model Position in the fluidic chart Length (mm)
1 J1 082-000055-00 B2 20
2 J2 082-000055-00 B2 20
3 J3 082-000055-00 A5 20
4 J4 082-000055-00 A5 20
5 J5 082-000055-00 A2 20
6 J6 082-000055-00 A2 20
7 J7 082-000055-00 B4 20
8 J8 082-000055-00 B4 20
9 J9 082-000055-00 B4 20
10 J10 082-000055-00 B4 20
11 J11 082-000055-00 B4 20
12 J12 082-000055-00 B4 20
13 J13 M90-100071--- C4 50
14 J14 M90-100071--- C4 25
15 J15 M90-100071--- C4 50
16 J16 M90-100071--- C4 25
17 J17 M6G-020006--- C3 15
18 J18 M6G-020009--- C3 15
19 J19 M6G-020006--- C3 15
20 J20 M6G-020009--- C3 15
21 J21 M6G-020006--- C4 15
22 J22 M6G-020009--- C4 15
23 J23 M6G-020006--- C4 15
24 J24 M6G-020009--- C4 15
25 J25 2000-20-03314 B4 1
26 J26 2000-20-03314 B4 1
27 J27 2000-20-03314 B4 1
28 J28 2000-20-03314 C4 1
29 J29 2000-20-03314 C4 1
30 J30 2000-20-03314 C4 1
31 J31 2000-20-03314 C4 1
32 J32 0030-20-13339 B4 1
F-5
046-001623-00
P/N 1.0

BC 3600

Hochgeladen von

Dokumentinformationen

Originaltitel

Copyright

Verfügbare Formate

Dieses Dokument teilen

Dokument teilen oder einbetten

Freigabeoptionen

Stufen Sie dieses Dokument als nützlich ein?

Sind diese Inhalte unangemessen?

Copyright:

Verfügbare Formate

BC 3600

Hochgeladen von

Copyright:

Verfügbare Formate

BC-3600

Auto Hematology Analyzer

Intellectual Property Statement

Release, amendment, reproduction, distribution, rent, adaptation and translation of this

. , are the registered trademarks or trademarks owned by

Responsibility on the Manufacturer Party

all installation operations, expansions, changes, modifications and repairs of this

the product is used in accordance with the instructions for use.

This equipment must be operated by skilled/trained medical professionals.

It is important for the hospital or organization that employs this equipment

THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,

This warranty shall not extend to:

any product of any other manufacturer.

Safety, Reliability and Performance

Assembly operations, extensions, re-adjusts, modifications or repairs are carried out

Personnel unauthorized by Mindray repairs or modifies the instrument.

Manufacturer: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.

Mindray Building, Keji 12th Road South, Hi-tech Industrial Park,

Fax: +86 755 26582934 26582500

EC-Representative: Shanghai International Holding Corp. GmbH(Europe)

Address: Eiffestrae 80, 20537 Hamburg Germany

1 Using This Manual 1-1

2 Analyzer Structure 2-1

4 Mechanical System 4-1

6 Hardware System 6-1

6.2 Data Board 6-5

8 Maintaining your analyzer 8-1

9 Error Information 9-1

To use this manual effectively, you need the following capabilities:

Comprehensive knowledge of circuit and fluidics;

Comprehensive knowledge of reagents;

Comprehensive knowledge of controls;

Comprehensive knowledge of troubleshooting;

Mastering the way to operate this analyzer;

Using basic mechanical tools and understand related terminology;

Using a digital voltmeter (DVM) and an oscilloscope;

Reading pneumatic/hydraulic schematics and understand related terminology.

If you want to See

BC-3600 and BC-3300

1.3. Conventions Used in This Manual

1.4. Special Terms Used in This Manual

When you read It means

press [ENTER] to select the desired item.

You will find the following symbols in this manual.

When you see Then

read the statement below the symbol. The statement is

When you see It means

CAUTION, CONSULT ACCOMPANYING

EXERCISE CAUTION WHEN WORKING

PROTECTIVE EARTH (GROUND)

FOR IN VITRO DIAGNOSTIC USE

It is important for the hospital or organization that employs this equipment to

Never use combustible gas (e.g. anesthetic) or combustible liquid (e.g.

Possible mechanical movement of the warned position may lead to personal

Be sure to dispose of reagents, waste, samples, consumables, etc.

Improper maintenance may damage the analyzer. Maintain the analyzer

For problems not mentioned in the service manual, contact Mindray

To prevent personal injury or damage to equipment components, remove

Electrostatic discharge may damage electronic components. If there is a

This equipment must be operated by skilled/trained medical professionals.

Samples, controls, calibrators and waste are potentially infectious. Wear

Make sure the analyzer is properly grounded.

Open vial mode