Beruflich Dokumente
Kultur Dokumente
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Revision #/Date: Approved By: Date
Revised:
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00-8/2005
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00-01/2008
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Revised 01/2008
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Description
Precautions:
Sample Preservation-Preparation:
Interferences:
Revised 01/2008
Apparatus
- Air incubator:
Thermostatically controlled to 20 C 1 C. Sealed to prevent
light entering.
- Stir plate
- Stir bar
- 25ml buret
- Volumetric pipettes, various
- Graduated cylinders, various
- Dissolved oxygen meter:
Orion meter using membrane electrode to take direct oxygen
measurements.
- Tubing and glass siphon (from dilution water to BOD bottles)
Reagents
- Alkali-Iodide-Azide solution:
Dissolve 500g NaOH (or 700g KOH) and 135g NaI (or 150g KI)
in
ultra pure water and dilute to l liter. Add 10g NaN 3 dissolved
in
40 ml of ultra pure water.
CAUTION: this solution is highly corrosive.
Revised 01/2008
- Sodium Sulfite solution: Dissolve 1.575g Na2SO3 in 1-L ultrapure
water. Prepare daily.
Procedure
1.1 Add two BOD nutrient buffer pillows to 19L of ultra pure water:
(Cat. # 14863-98 to 19L / Cat. #14862-98 to 6 L). Aerate for
15-30 minutes using lab grade compressed air.
2.1 Pour off three incubation bottles with the freshly prepared
dilution water.
Cap and set one aside.
Revised 01/2008
2.6 Titrate remaining 200ml with 0.0250N sodium thiosulfate to a
light straw
color. Add 1-2 ml of starch indicator and continue titration to
a clear
endpoint.
3.1 Put DO probe into bottle previously capped and set aside
(step 2.1)
3.2 Turn knob from off position to C. Let reading stabilize about
15
minutes.
3.3 Turn to MG/L CAL and enter the DO of the dilution water. Use
the
three buttons on the display set to scroll to the correct
numbers.
3.4 Turn knob to MG/L. After the meter has stabilized, it will give
two beeps
and a reading. It is then ready to measure DO results for
BOD/CBOD.
4.2 Samples should be checked for total residual chlorine using Hach
chlorine powder pillows. If chlorine is present, it should be removed
by addition of sodium sulfite.
Revised 01/2008
4.2.3 Add to neutralized sample a proportionate amount of
sodium sulfite. Mix well.
4. River Water:
Prepare bottles using 75 to 300mls of sample.
Revised 01/2008
5.2 Fill with dilution water by using the siphon apparatus under the
level of the sample. Rinse after each bottle. Measure initial DO
with oxygen probe and replace displaced water with dilution
water. This measurement should be made as quickly as
possible after dilution.
5.3 Cap tightly making sure no air bubbles are entrapped in the
incubation bottle, cover with plastic cap and place in the
incubator. A dilution water blank should be run with each sample set. If
samples have been seeded, a seeded dilution water blank
should be run. If CBODs are analyzed, a dilution water blank with
nitrification inhibitor should be run.
6.0 Calculations:
6.1.1 The difference between the final and initial D.O. must be
>2.0 and
BOD CALCULATIONS
UNSEEDED SAMPLES:
Revised 01/2008
BOD mg/L = (Initial DO-Final DO) X 300
mls sample
SEEDED SAMPLES:
If all sample depletions are <2.0 the result is BDL. If the largest
sample volume used was not 300 mls. YOU MUST ADJUST THE
DETECTION LIMIT!!!!!
Calculation:
(2.0) X 300
mls sample* * This is the largest volume of sample used.
If all Final DOs are <1.0 the result will be reported as >.
To calculate this, use the initial DO of the dilution with the *smallest
sample volume.
STANDARD CALCULATION:
Waste Disposal
Quality Control
Revised 01/2008
The unseeded blanks should not exceed a BOD of 0.2mg/L.
References
Revised 01/2008