INFIRMARY HEMATOLOGY

DOCUMENT CODE: TP-SCMH-1G-SR

REVISION INTIAL DOCUMENT

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EFFECTIVITY DATE:
TITL TECHNICAL PROCEDURE: HORIBA ABX MICROS ES60
E
OPERATIONAL CONDITIONS

*The operation of the ABX Micros ES 60 should be restricted to indoor

location use only.

LOCATION

Place the instrument on a clean and leveled table or workbench. Keep

in mind that the instrument weighs approximately 17 Kgs.

Avoid exposure to sunlight.

Place the instrument where it is not exposed to water or vapor.

Place the instrument where it is free from vibration or shock.

Provide a space of at least 20 cm (8 in.) at the back of the instrument

for a proper ventilation and an easy access to connections.

The Power switch and Power supply connection should always be

accessible. When positioning the system for operational use, leave the
required amount of space for easy accessibility to these items.

Grounding

Proper grounding is required when installing the system. Check the

wall outlet ground (earth) for proper grounding to the facilities electrical
ground. If you are unsure of the outlet grounding, contact the facilities
engineer to verify the proper outlet ground.

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 INFIRMARY HEMATOLOGY
DOCUMENT CODE: TP-SCMH-1G-SR

REVISION INTIAL DOCUMENT

NUMBER:
EFFECTIVITY DATE:
TITL TECHNICAL PROCEDURE: HORIBA ABX MICROS ES60
E
Humidity and Temperature Conditions Instrument operating
temperature

From +16C (+61F) to +30C (+86F), with a relative humidity of 85%

maximum, without condensation.

Electromagnetic Environment Check

The instrument has been designed to produce less than the accepted
level of electromagnetic interference in order to operate in conformity with
its destination, allowing the correct operation of other instruments also in
conformity with their destination. In case of suspected electromagnetic
noise, check that the instrument has not been placed in the proximity of
electromagnetic fields or short wave emissions, e.g. Radar, X-rays, Scanners,
Cell phones, etc.

Main Power Supply

Grounding is required. Check that earth wall-plug is correctly

connected to the laboratory groundingSystem. If there is no such system, a
ground stake should be used. Use only main supply cable delivered with the
instrument. Main power supply voltage fluctuations must not exceed +/- 10%
of the nominal voltage.

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 INFIRMARY HEMATOLOGY
DOCUMENT CODE: TP-SCMH-1G-SR

REVISION INTIAL DOCUMENT

NUMBER:
EFFECTIVITY DATE:
TITL TECHNICAL PROCEDURE: HORIBA ABX MICROS ES60
E

SAMPLE ANALYSIS

1. Before switching on the instrument, please ensure:

a. Check the level of all reagents

b. Empty the waste container

c. Contains sufficient paper in printer

2. Switch on the ABX Micros ES 60 by pressing the button at the back of

the instrument

a. When the instrument is switched on, it will go for temperature

adjustment cycle, initiate the Startup Cycle by pressing the
startup cycle button.

i. After the Startup Cycle, a blank reading will be shown.

Check the admissible limits of the machine .

ADMISSIBLE LIMITS
WBC<0.3 RBC<0.02 HBG <0.3 PLT <10

b. Once the blank count is within acceptable range, press the check
button. This will bring you to the Login page.

c. Press L on screen.

d. Press the check sign on the screen.

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DOCUMENT CODE: TP-SCMH-1G-SR

REVISION INTIAL DOCUMENT

NUMBER:
EFFECTIVITY DATE:
TITL TECHNICAL PROCEDURE: HORIBA ABX MICROS ES60
E
3. Perform internal quality control by using the Blood Control ABX Minotrol
16 (Low, Normal and High) daily and verify the values by pressing the
QC icon.

4. To enter patients demography, press the sample ID icon on the right

side of main screen. This includes Sample ID, Patient ID, Name, First
Name, Physician, Type (Man/ Woman/ Child) and date of birth.

5. Press the check sign to validate.

6. Using the patients EDTA whole blood. Mix the sample properly by
gently rotating it upside and down at least 10 times.

7. Remove the Cap of the Sample Tube and place it to the sample probe.

8. Press the manual sampling bar for the machine to aspirate the blood.

9. Wait for the machine to release the result. This takes 1 minute.

MAINTENANCE & TROUBLESHOOTING

REAGENTS REPLACEMENT

Access: Main screen > Maintenance menu > Reagent (tab)

An reagent alarm is triggered and display in the Status screen if:

the reagent is expired

the remaining number of tests is lower than 10

the diluent container is empty.

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DOCUMENT CODE: TP-SCMH-1G-SR

REVISION INTIAL DOCUMENT

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TITL TECHNICAL PROCEDURE: HORIBA ABX MICROS ES60
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Then, the ABX Minidil LMG must be changed :

1. Unscrew the stopper assembly of the new container using the

opening tool provided with your Instrument

2. Remove the stopper assembly straw from the empty container

and insert it into the new one.

3. In the Reagent tab, select the DILUENT radio button.

4. Press the Replace button.

5. Press Validate in the dialog box.

6. In the Edit reagent screen, press Edit in the contextual toolbar.

7. Press the Lot field to activate it.

8. Read the diluent barcode label with the barcode reader or type
the lot number.

9. Using the virtual keyboard or an external keyboard (if one is

connected). The instrument searches diluent information in the
reagent database.

10. When diluent information are displayed, press Validate in

the contextual toolbar.

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DOCUMENT CODE: TP-SCMH-1G-SR

REVISION INTIAL DOCUMENT

NUMBER:
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TITL TECHNICAL PROCEDURE: HORIBA ABX MICROS ES60
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11. In the Reagent tab, press the Prime button to launch a
prime cycle.

TO REPLACE A REAGENT BOTTLE

The Lyse reagent is provided in a bottle (ABX Minilyse LMG or ABX Alphalyse)
or in a small pack (ABX Alphalyse 360):

To replace the Lyse bottle: follow the procedure below.

To replace the Lyse small pack: refer to the To Replace the ABX
Alphalyse 360 pack (Optional) chapter.

1. Open the reagent compartment flap cover.

2. Remove the empty bottle from the reagent compartment.

3. Install the reagent straw and the bottle stopper on the bottle.

4. Connect the tube to the reagent bottle.

For the Cleaning bottle: connect the blue tube.

For the Lyse bottle: connect the white tube.

5. Place the reagent bottle in the reagent compartment

6. Close the reagent compartment flap cover.

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REVISION INTIAL DOCUMENT

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7. Close the reagent compartment flap cover.

TO REPLACE THE INTEGRATED TICKET PRINTER PAPER

1. Lift the lever.

2. Lift the printer cover.

3. Remove the old paper roll.

4. Load new paper roll.

5. Close the printer cover by pressing simultaneously on both ends of the

cover. A click should be heard.

CONCENTRATED CLEANING

IT IS RECOMMENDED THAT CONCENTRATED CLEANING SHOULD BE

PERFORM TWICE A WEEK (MONDAY AND THURSDAY).

Access: Main screen > Maintenance menu > Serv. (tab) >
Customer services (button) > Hydrau. (tab)

Prepare a 5 mL syringe, a bottle brush and ABX Minoclair or bleach

solution containing 12% active chlorine.

1. Press the Concentrated cleaning radio button.

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DOCUMENT CODE: TP-SCMH-1G-SR

REVISION INTIAL DOCUMENT

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EFFECTIVITY DATE:
TITL TECHNICAL PROCEDURE: HORIBA ABX MICROS ES60
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2. Press the Start cycle button and follow the steps that appears on the
screen.
This message is displayed: "This cycle takes 10 minutes; it cannot be
cancelled until it has finished; are you sure that you want to run this
cycle? If so, open the front door and press confirm to continue.".

3. Open the front cover.

4. Press Validate in the dialog box.

This message is displayed: "Put 3 mL of MINOCLAIR in WBC chamber

and validate to continue.".

5. Dispense the bleach solution into the WBC chamber (or into the hole
on the top of the cover that it goes into the chamber).

6. Press Validate in the dialog box.

This message is displayed: "Put 3 mL of MINOCLAIR in RBC chamber

and validate to Continue.".

7. Dispense the bleach solution into the RBC chamber (or into the hole
on the top of the cover that it goes into the chamber).

8. Press Validate in the dialog box.

This message is displayed: "Close the front cover and validate to

continue.".

9. Close the front cover.

10. Press Validate in the dialog box.

The cleaning cycle begins and a cleaning time is displayed. This

concentrated cleaning cycle involves different cycles, backflush,

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DOCUMENT CODE: TP-SCMH-1G-SR

REVISION INTIAL DOCUMENT

NUMBER:
EFFECTIVITY DATE:
TITL TECHNICAL PROCEDURE: HORIBA ABX MICROS ES60
E
aspiration, rinsing, which allow a good cleaning of the chambers and
apertures.

11. When the procedure is complete, perform a startup cycle to

check that the reference blank parameters are within their limits.

a. Press the Status button to open the Status screen.

b. Press the Startup button.

12. Re-run quality control and check that the results are within the
assay limits listed on the controls. Once the startup cycle has passed,
sample analysis may begin.

TURNING OF THE MACHINE

1. Press the exit button on the screen, to show the exit screen.

2. As the 3 icons appeared, press the shutdown button and validate.

3. Wait the machine to display You can turn off the machine before
flipping the switch button at the back.

4. Turn off the AVR and unplug.

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