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Biology

Osmosis Lab Report

Chatthanayongphakdi, Natthapat, Pond

Wachapatthana, Ingkarat, Ing

Singlamlert, Chomphunik, Fay

Petchpadoong , Thitaporn, Genie

Tavichai, Chinorod, Chino

1004

Date : March, 10th 2017


Introduction: attached in the back

Purpose: To observe the effects and changes of objects due to the process of osmosis by its hypotonic
and hypertonic features.

Question: How will soaking the Jelly in distilled water affect the size of the candy?
Hypothesis: If the jelly is in distilled water, then the size of jelly will be bigger after being
soaked.
Question: How will soaking the Jelly in concentrated salt water affect the size of the candy?
Hypothesis: If the jelly is in salt water, then the size of jelly will be smaller after being soaked.

Question: How will soaking the Potato in distilled water affect the size?
Hypothesis: If the potato is in distilled water, then the size of potato will be bigger after being
soaked.

Question: How will soaking the Potato in concentrated salt water affect the size?
Hypothesis: If the potato is in salt water, then the size of potato will be smaller after being
soaked.

Materials:

6 Beakers (3 for jelly, 3 for potato)


Electronic Balance
Knife
Distilled water
Weighing paper
Concentrated salt water
Markers
3 Jellies
Graduated cylinder
3 Potato Pieces
Lab Coat
groups bring their own
Calculator
Goggles
calculator
Methods:
Collect the materials that your group needs (3 Jelly and Half a Potato)
Use the knife to cut the potato into 3 equal pieces
Use the electronic balance to find the mass of your jelly. Remember to lay down a piece of weighing
paper and set to zero.
Record your data
Record descriptive observations about the jelly prior to the experiment.
Measure the initial volume of the jelly and the potato and record your data.
Fill your 1st beaker halfway with distilled water. Put one of your jelly into the beaker. Set the beaker
aside for one hour. Label the beaker using marker.
Fill your 2st beaker with concentrated salt solution halfway. Put your 2nd jelly into that beaker. Set
beaker aside for one hour. Label the beaker using marker.
Put your 3rd jelly into an empty beaker without putting anything in it. That should be your control.
Put the 4th beaker halfway with distilled water. Put one of your potato pieces into the beaker. Set
the beaker aside for one hour. Label the beaker.
Put the 5th beaker halfway with concentrated salt solution halfway. Put your second potato pieces
into that beaker and set it aside for an hour. Label the beaker.
Place your last piece of potato pieces into the last beaker, empty. That is your control for the
potato. Label the beaker.
Make sure you label all the beakers distilled water or concentrated salt solution with some
markers.
After one hour, come back and look at each of the beakers and record the data volume and mass of
both the jellies in two beaker and both the potato in two other beakers.
Also record the descriptive data for what the jelly and the potato look like now after being soaker
for an hour.
Calculate the percent change in the size of each jelly and potato
a. Percent change in volume
b. Percent change in mass

DATA TABLE A:

Jelly 1 (Color): Jelly 2 (Color):

Dimensions Before After Soaking Before After soaking in


Soaking in Distilled Soaking Salt Water
Water

Volume 2.0ml 3.0 ml 1.8ml 1.0 ml

Mass 2.66g 4.17g 2.90ml 1.56 g


DATA TABLE B:

Potato 1 (Yellow): Potato 2 (Yellow):

Dimensions Before After Soaking Before After soaking in


Soaking in Distilled Soaking Salt Water
Water

Volume 4.0 ml 5.0 ml 4.0 ml 3.0 ml

Mass 4.10 g 4.78 g 4.31 g 3.54 g

DATA TABLE C:

Jelly 1: (Red) Jelly 2: (Red)

Descriptive Observations Before Soaking in Descriptive Observations Before Soaking in


Distilled Water: It is in semicircle shape. It is Salt Water: It is in semicircle shape. It is soft
soft and sticky in the same time and it smell and sticky in the same time and it smell good
good

Descriptive Observations After Soaking in Descriptive Observations After Soaking in


Distilled Water: The jelly will swell because Salt Water: The jelly will shrink because
more water go into the jelly to make the more water go out of the jelly to make the
balance of concentration balance of concentration in the environment.
DATA TABLE D:

Potato 1: (Yellow) Potato 2: (Yellow)

Descriptive Observations Before Soaking in Descriptive Observations Before Soaking in salt


distilled water : The potato is slice into dice water : The potato is slice into dice shape, hard
shape, hard feeling and slippery. feeling and slippery.

Descriptive Observations After Soaking in Descriptive Observations After Soaking in salt


Di distilled water : The potato become bigger and water : The potato is yellow color, dice shape,
th it have a lighter colour. Because the potato is hy sm smooth surface texture. The potato is
hypotonic to the distilled water so the water go hypotonic comparing to the salt water which is
transport into the potato. hypertonic It also getting smaller.

DATA TABLE E:

Jelly 1 (Red): Soaked in Jelly 2 (Red): Soaked in Salt


Distilled Water Water

Percent Change in Volume +50 % -44.4%

Percent Change in Mass + 56.8% -46.2%


DATA TABLE F:

Potato 1 (Yellow): Soaked Potato 2 (Yellow): Soaked


in Distilled Water in Salt Water

Percent Change in Volume + 25% -25%

Percent Change in Mass +16.6% -17.9%

Bar Graph: Make bar graphs to show the percent change in mass and volume for both the jelly and the
potato.
Analyzing Results Questions:

1. What happened to the jellies after being soaked in distilled water and salt water for an hour? Why?
Be descriptive and detailed in your answer. Use your knowledge of membrane transport.
- After two jellies are being soaked in the solution for an hour, it changes the size of its shape.
For jelly in the distilled water, the size is become bigger by increasing 50% in volume and
56.8% in mass because jelly has more concentration of solute than distilled water; jelly is
hypertonic solution to pure water. Therefore, water from outside jelly will come inside jelly
as water move from high to low concentration. For jelly in the salt water, the size is become
shrink by decreasing -44.4% in volume and -46.2% in mass because jelly has less
concentration of solute meaning that there is more water concentration inside jelly than in
the salt water. So water will move out of the cell and jelly size become smaller in order to
make balance concentration of solute and solvent between jelly and water outside.

2. What happened to the potato after being soaked in distilled water and salt water for an hour? Be
descriptive and detailed in your answer. Use your knowledge of membrane transport.
- When potato soaked in distilled water for an hour, it increases in volume by 25% and mass
by 16.6%. This is because of osmosis as potato and pure water have different concentration
of solute and solvent. Potato is hypertonic solution to distilled water, so water goes inside
potato and make potato increase in size.
- Potato 2 in the salt water become smaller after soaked in the salt water. Due to the
hypertonic solution of salt water (have more solute than solvent), the liquid inside potato
goes out to salt water for making equilibrium of between potato and water outside.

Conclusion:

From this experiment, we have observed how an objects size, shape, mass, and volume can be
affected by the process of osmosis. The potatoes and the jellies inside the hypotonic solutions
(distilled water) have an increase in both volume and mass. Having a +50% and +56.8% change in
volume and mass for the jelly, and a +25% and +16.6 % change for the potato respectively. Whereas,
the potatoes and the jellies inside the hypertonic solutions (saltwater) have a decrease in both its
mass and volume. Having a -44.4% and -46.2% change in the second jellys volume and mass, and a
-25% and -17.9% change for the second potato respectively.

Work log:

Name Responsibility

Genie data table

Fay Making data table, helping and observing


during the process in the lab, organizing the lab
report

Ing Purpose, Conclusion

Pond I help fill in data table

Chino Hypothesis, data table, analysis question