N Methodological Genetic relationship Morphological and

Remark
o applications in different Sheep molecular genetic
population diversity of Syrian
of Bangladesh based indigenous goat
on Microsatellite populations (Hassen et
markers al.,2015)
4 DNA extraction - DNA was extracted - DNA extraction procedure -used
following a salting described for peripheral blood different
out protocol after lymphocytes (PBL) stored with protocols
IAEA (2004) Urea (Tapio et al., 2007).
-genotyping results --- DNA concentrations were
were estimated by a Nano-DNA
processed by the spectrophotometer
GeneMapper v3.7
program which
determines the allele
sizes of each animal
5 PCR - All of 140 DNA - in a thermal cycler Similar
amplification and sample were - (GeneAmp PCR System procedure
genotyping amplified in 2700 96-well cyclers)
GeneAmp PCR - 12 microsatellite
system 9700 PCR markers selected from
machine using FAO/ISAG panel list (FAO,
-18 pairs of 2004)
microsatellite primers
selected from FAO
recommendation