6.

0 Apparatus and Equipment

Materials

20 ppm of methyl red solution, activated carbon and distilled water

Apparatus

500 ml of volumetric flask, conical flask, electronic weighing balance, 0.45 m membrane filter,
and UV-Vis spectroscopy.

UV-Vis spectroscopy Computer

Methodology

UV-Vis Spectroscopy Standard Procedure for measuring absorption spectrum and

determining of maximum wavelength, max

1. The UV-Vis Spectroscopy was switched on and the instrument was allowed to
initialize for a few minutes.
2. The software of Lambda 25 was opened and the wavelength was set to 420, m for
analysis.
3. Rinse one of the cuvettes with distilled water and fill it with the blank sample. The
cuvette that was filled with of the blank sample was put into the sample
compartment and set as the reference solution.
4. Then, the other cuvette was filled with of 8ppm methyl red sample solution and
placed the cell in the sample compartment
5. The sample was analyzed and the maximum wavelength, max was recorded.
6. The machine was set up and ready for analysis.

The calibration curve

1. The same steps were repeated as in the determination of maximum wavelength,

max by using 500 ml of methyl red solution with 20 ppm concentration.
2. The methyl red solution was diluted into for different concentrations which are
8ppm, 6ppm, 4pmm and 2pmm respectively.
3. The maximum wavelength, max and the designated concentration was set under the
concentration section. Then, the cuvette that was filled with of the sample was
placed in the cell compartment.
4. Then, the calibration curve of absorption vs concentration was plotted.

For catalyst dosage study

1. 50ml of methyl red solution with 10 ppm concentration was prepared into three
different conical flask
2. The activated carbon was weight 2 g, 4 g and 6 g respectively and poured into the
conical flask that contain 50ml of methyl red solution
3. Then, the mixture was stirred by orbital shaker at 150 rpm for 30 minutes.
4. The mixture was filtered with 0.45 m membrane filter in order to separate the
activated carbon from methyl red solution sample.
5. The prepared sample was analyzed by UV-Vis spectroscopy and the final
concentration was recorded in CD-ROM.

Shut-down procedure
 1. The application program was closed and the cuvette was removed from the sample
compartment
2. The cuvette was cleaned and placed into it case.
3. The UV-Vis spectroscopy was switch off and the computer was shut down.

Appendices
Figure1.1Methyl Red Dye Sample Figure1.2 Dye sample after adsorption by
activated carbon

Figure1.3 activated carbon