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MULLER KAUFFMANN BROTH BASE

w/ BRILLIANT GREEN & NOVOBIOCINE


(MKTTN) (ISO 6579:2002)
Liquid medium for the selective enrichment of
Cat: 1173 Salmonella

FORMULA IN GRAMS PER LITER


Sodium thiosulfate anhydrous30,5.=.pentahydrated... 47.8 Calcium Carbonate.38.70
Enzymatic digest of casein .. 8.60 Ox Bile ..4.78
Beef Extract.. 4.30 Sodium Chloride2.60
Novobiocine... 0.04 Brilliant Green0.0096

Final pH 8.2 0.2 at 25C


Preparation
Dissolve 89,53 grams of the medium in one liter of distilled water. Heat with frequent agitation for one minute. Do not
STERILIZE IN autoclave. Cool to 45C. Add the supplements as required.:Add 20 ml of a solution of 20 g of iodine,
and 25 g of potassium iodide / in 100 ml of distilled water.

Uses
Muller-Kauffmann Broth Base with Brilliant Green & Novobiocine is recommended by the ISO 6579:2002 norm to
be used a selective enrichment broth of Salmonella spp. Calcium carbonate is a neutralizer which absorbs toxic
metabolites. The Bile Salts and the Brilliant Green inhibit gram positive, and most gram negative, bacterial growth
Microorganisms with tetrathionate reductase enzyme will grow and multiply in this medium.

Bibliography
Kauffmann, F. 1935. Weitere erfahrungen mit dem kombininierten anreicherungsverfahren fur Salmonella bazillen.
Ztschr. F. Hyg. 117: 26-32.
A manual for recommended methods for the microbiological examination of poultry and poultry products.
1982. ISO 6579:2002.

MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a temperature
of 37 1 C, and observed after 24 3 hours.

Microorganisms Inoculum Growth


Concentration 6 hours 24 hours
Escherichia coli ATCC 25922 99% < 30% < 5%
Salmonella typhimurium ATCC 14028 1% > 70% > 95%

www.condalab.com Dehydrated Culture Media