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Larvaecidal Property of Eucalyptus globulus (Blue Gum Tree)

Leaf Extract on Culex spp. (Mosquito)

A Research Proposal presented to the


faculty of the College of Medical Technology
Southwestern University PHINMA
Cebu City, Philippines

By:

Alayon, Christian Marc C.


Gacott, Evan Jane D.
Hao, Norlane Jane Y.
Lapuhapu, Jezaniah M.
Padogdog, Krisha Ace T.

Eleanor D. Apita, RMT


Research Adviser

October 2016
25

REFERENCES

Anderson and Harrington (2006). Mosquito Biology for the


Homeowner.http://www.entomology.cornell.edu/MedEnt/Mosqui
toFS.html (Retrieved on October 2016)

Bahr and Johnson (1995). Mosquito. In Colliers Encyclopedia (Vol.16).


New York: P.F. Collier, L. P.

Britannica Online Encyclopedia (2006). Insecticide.


http://www.britannica.com/EBchecked/topic/289077/insecticide
(Retrieved on September 2016)

Doctor Ayur (2008). Eucalyptus globulus a traditional aboriginal


remedy.http://www.doctorayur.com/index.php?
option=content/eucalyptus-globulus (Retrieved on September
2016)

Science Direct (2008). Eucalyptus essential oil as a natural pesticide.


http://www.sciencedirect.com/science/article/pii/S03781270800
(Retrieved on September 2016)

World News (1998). Blue Gum Eucalyptus. From website:


http://www.wn.com/Blue_Gum_Eucalyptus(Retrieved on
September 2016)

Padam Parkash Bhojvaid and Shailendra Kaushik (2014). Eucalyptus


in
India.http://s3.amazonaws.com/academia.edu.documents/3977
2019/Eucalypts_-envis-book.pdf (October 2016)
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27

Appendix A

Pictures

Figure 1. Mosquito Larvae

Figure 2. Eucalyptus globulus tree

Research
4
Procedures
3 2
Flow Chart

Writing of transmittal letters to


the dean of the College of Authentication of the
Medical Technology and the Eucalyptus globulus leaves at
dean of College of Pharmacy, the Department of Agriculture.
for the usage of the laboratory
and the Soxhlet Extration
machine.

Setting of breeding spot for Collection and drying of


the collection of Culex Eucalyptus globulus leaves.
larvae at a grassy dark mini The dried leaves are then used
forest at Bulacao, Tabunok, for extraction using the
Talisay City. Soxhlet Extration machine.

Three beakers are filled with 50


larvae each:
-Beaker 1- poured with solution 1
The Culex larvae and the
(25% conc.)
Eucalyptus globulus leaf
-Beaker 2- poured with solution 2
extract are gathered for
(50% conc.)
the mortality test.
-Beaker 3- poured with solution 3
(100% conc.)

Observe and record mortality


within 10 minutes, 20 minutes, and
30 minutes, and 60 minutes.
Repeat for trials two and three.

Reporting of results

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