APNDICE B

Datos para determinacin de antocianinas monomricas totales (AT)

AGUA repeticin lmax=510 AT(mg/100mL)

pH 1 Abs(510) Abs(700)
1 0.6974 0.1462 13.52442677
2 0.6509 0.1126 15.50769765
3 0.527 0.0893 12.25809467
pH 4.5 MEDIA 13.76340636
1 0.4333 0.2292
2 0.2937 0.1534
3 0.2483 0.1252

ETANOL
(96%) repeticin lmax=510 AT(mg/100mL)
pH 1 Abs(510) Abs(700)
1 0.9395 0.0039 48.03684684
2 0.92 0.0024 47.17184461
3 0.9196 0.037 45.76329368
pH 4.5 MEDIA 46.99066171
1 0.1242 0.0105
2 0.1135 0.003
3 0.1241 0.0245

ETANOL-AGUA repeticin lmax=510 AT(mg/100mL)

(50:50) pH 1 Abs(510) Abs(700)
1 0.6598 0.0062 46.34385824
2 0.6623 0.006 47.06079703
3 0.7129 0.0065 51.08188848
pH 4.5 MEDIA 48.16218125
1 0.0695 0.0106
2 0.0607 0.0083
3 0.0624 0.0115
ETANOL-AGUA repeticin lmax=510 AT(mg/100mL)
(70:30) pH 1 Abs(510) Abs(700)
1 0.9906 0.0116 70.63405601
2 0.9902 0.0098 71.67829294
3 0.9812 0.0188 70.33792912
pH 4.5 MEDIA 70.88342602
1 0.082 0.0094
2 0.0766 0.016
3 0.0682 0.0084

ETANOL
CIDO repeticin lmax=510 AT(mg/100mL)
pH 1 Abs(510) Abs(700)
1 0.9472 0.0094 66.42593705
2 0.8069 0.0077 55.25883618
3 0.9536 0.0464 65.64665576
pH 4.5 MEDIA 62.44380967
1 0.096 0.0106
2 0.102 0.0119
3 0.0821 0.0173

ETANOL-AGUA repeticin lmax=510 AT(mg/100mL)

(70:30) a 30C pH 1 Abs(510) Abs(700)
1 0.9898 0.0075 72.83162924
2 0.9896 0.0032 73.51739678
3 0.9899 0.0041 74.24992119
pH 4.5 MEDIA 73.5329824
1 0.0568 0.0091
2 0.0514 0.0084
3 0.0401 0.0071

ETANOL-AGUA repeticin lmax=510 AT(mg/100mL)

(70:30) a 35C pH 1 Abs(510) Abs(700)
1 0.9953 0.0202 77.83461512
2 0.911 0.0132 71.11721041
3 0.9806 0.0119 76.22150285
pH 4.5 MEDIA 75.05777613
1 0.0622 0.0859
2 0.0764 0.0912
3 0.0765 0.0859
Contenido de antocianinas monomricas (AT) totales presentes extractos de zarzamora (media
DE, n=3).
Sistema Extractante AT
(mg/100ml)
1 Agua 15.421.74a

2 Etanol 53.250.84b

3 Etanol:agua 58.540.82b
50/50
4 Etanol:agua 64.660.70b
70/30
5 Etanol 60.990.39b
acidificado
La concentracin de antocianinas monomricas totales (AT) en el extracto se expresa como mg de cianidina-
3-glucsido/100mL de extracto.