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Molecules(of(Life(
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FEBS3+!Meeting!
organized!by!the!Slovenian!Biochemical!Society,!
Croatian!Society!of!Biochemistry!and!Molecular!Biology,!
Hungarian!Biochemical!Society!&!
Serbian!Biochemical!Society!
!
and!
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11th!Meeting!of!the!
Slovenian(Biochemical(Society!
!
September!16B19,!2015!
Portoro,!Slovenia!
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Book$of$Abstracts$
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Book$of$Abstracts$of$the$FEBS3+$Meeting$Molecules(of(Life$organized$by$the$Slovenian$
Biochemical$Society,$Croatian$Society$of$Biochemistry$and$Molecular$Biology,$Hungarian$
Biochemical$Society$&$Serbian$Biochemical$Society$
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Editors:$Janko$Kos,$Nataa$Poklar$Ulrih$
Technical(Editors:$Miha$Pavi,$Mihaela$Skrt$
Edition:$324$pages,$380$copies$
Publisher:$Slovenian$Biochemical$Society,$Ljubljana,$september$2015$
Cover(page(design:$Miha$Pavi$
Logo(design:$Ana$Flaker$
Printed(by:$Reflect$d.o.o.,$Brezovica$pri$Ljubljani$
Photos:$Slovenian$coast$(www.slovenia.info;$Archy$Torkar);$Regatta,$Portoro$
(www.slovenia.info;$Ale$Fever);$Piran$Cape$(www.slovenia.info;$Ubald$Trnkoczy);$
Limestone$formation,$kocjan$cave$(www.slovenia.info;$D.$Mladenovi)$
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CIP$a$Kataloni$zapis$o$publikaciji$$
Narodna$in$univerzitetna$knjinica,$Ljubljana$$
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577.1(082)(0.034.2)$$
$
FEBS3+$Meeting$(2015$;$Portoro)$$
$$$$$$Molecules$of$life$[Elektronski$vir]$:$book$of$abstracts$/$FEBS3+$Meeting$and$11th$
Meeting$of$the$Slovenian$Biochemical$Society,$September$16a19,$2015,$Portoro,$
Slovenia$;$organised$by$Slovenian$Biochemical$Society$...$[et$al.]$;$[editors$Janko$Kos,$
Nataa$Poklar$Ulrih].$a$El.$knjiga.$a$Ljubljana$:$Slovenian$Biochemical$Society,$2015$$
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ISBN$978a961a93879a1a7$(pdf)$$
1.$Gl.$stv.$nasl.$2.$Kos,$Janko,$1959a$3.$Slovensko$biokemijsko$drutvo.$Meeting$(11$;$
Portoro)$4.$Slovensko$biokemijsko$drutvo$$
281121280$$

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 FEBS3+!Meeting!Molecules(of(Life!//!September!16B19,!2015!//!Portoro,!Slovenia!
under!the!auspices!of!FEBS!//!organized!by!the!Slovenian!Biochemical!Society,!Croatian!Society!of!Biochemistry!and!
Molecular!Biology,!Hungarian!Biochemical!Society!&!Serbian!Biochemical!Society!
!
S37!
Carbonylation(of(HSA(with(methylglyoxal(affects(its(copper(II)(binding(
affinity(
!
1,2 1 1 1 1
Ana!Penezic ,!Ivan!Pavicevic ,!Vesna!Jovanovic ,!Jelena!Acimovic ,!Ljuba!Mandic !
1
University&of&Belgrade,&Faculty&of&Chemistry,&Chair&of&Biochemistry,&Belgrade,&Serbia!
2
Institute&for&the&Application&of&Nuclear&Energy&INEP,&Belgrade,&Serbia!
anap@inep.co.rs!!
Carbonylation!of!human!serum!albumin!(HSA)!with!methylglyoxal!affects!its!copper(II)!
binding!affinity!
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The! effects! of! carbonylation! with! methylglyoxal! on! HSA\'s! copper(II)! binding! affinity! and!
copper(II)! release! from! copperBHSA! complexes! were! studied.! Carbonylation! causes!
conformational! changes! in! HSA! molecule,! Cys34BSH! content! decrease,! copper! binding!
affinity!decrease!and!release!of!copper!from!copperBHSA!complexes.!The!percent!reduction!
ratio!(Cys34BSH!group!content/HSA!bound!copper)!upon!HSA!carbonylation!and!oxidation!
was!the!same,!indicating!that!binding/release!of!copper(II)!ions!depends!mainly!on!the!redox!
state! of! Cys34BSH.! Observed! changes! were! tested! in! diabetic! group.! Cys34BSH! and! HSAB
bound! copper(II)! ions! contents! are! significantly! lower! (! 0.4570.081! mol! SH/mol! HSA,!
1.070.01!mol/mol!HSA,!resp.)!compared!to!the!control!group!(0.6090.027!mol!SH/mol!
HSA,! 1.340.01! mol/mol! HSA,! resp.).! Strong! correlations! between! HSABSH! content! and!
HbA1c! (R=B0.803),! and! between! HSABbound! copper(II)! and! the! HSABSH! content! (R=0.841)!
were!found.!Thus,!HSA!carbonylation!lead!to!decrease!of!HSABSH!content!and!to!increase!of!
free! copper(II)! ion! level! in! serum,! contributing! to! further! enhancement! of! oxidative! and!
carbonyl!stress!in!diabetes.!
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