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The Use of Molecular Biology Techniques in a Search for Varieties Resistant to ICCO Secretariat
Witches Broom Disease of Cocoa
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LastUpdatedon02May2012
InternationalCocoaOrganization
06BP1166Abidjan06
Fiveandahalfyearsaftersix Cted'Ivoire
DURATION:
monthextension.
BRAZIL, Tel:+22522514950/51
LOCATION: ECUADOR, Fax:+22522514979
PERU Email:info@icco.org
NATUREOFPROJECT: Sustainablecocoaproduction

ESTIMATEDTOTALCOST: US$3,191,824 Projects Documents

FINANCINGCOMMITEDBYCFC: US$816,197 Browsethedocumentsfromall

ICCOProjects

COFINANCING: US$800,000

COUNTERPARTFINANCING: US$1,575,527
ICCO Agreements

ComissaoExecutivadoPlano TheICCOInternationalCocoa
PROJECTEXECUTINGAGENCY(PEA): LavouraCacaueira(CEPLAC), Agreementisavailabletodownload
Brazil
inAcrobatPDFformat.
InternationalCocoa
PROJECTSUPERVISORYBODY:
Organization(ICCO) GototheICCOAgreementspage

PROJECTSTARTINGDATE: April2000

ICCO Membership Procedure

COMPLETIONDATE: September2005
Theproceduresforbecominga
memberoftheInternationalCocoa
BriefDescription
OrganizationareprovidedinArticles
Themainactivitiesoftheprojectwereconstructionofageneticlinkagemapidentificationandcharacterizationofmolecular
markersassociatedwithresistancetowitchesbroomdiseaseidentificationofqualitytraitloci(QTL)relatedtoagronomic 52to57oftheInternationalCocoa
traitsuseofrecurrentselectiontoobtainimprovedpopulationsbackcrossingincocoaandgermplasmevaluation. Agreement,2010.

ProjectObjectives HowtoBecomeanICCOMember
The main aim of the project was to apply molecular biology techniques in cocoa Germplasm evaluation, in InternationalCocoaAgreement
particularusingmolecularmarkersatDNAlevel,toimproveknowledgeontherelationshipsamonggenotypes 2010
includingstudiesonheterozygosity,pedigree,characterizationofgenescontrollingtheinheritanceofeconomic
traits and identification of genes controlling disease resistance. This process would lead to accelerated
developmentandreleaseofnewcocoaplantingmaterialswhichwouldbemoreuniform,moreproductiveand
moretoleranttodiseases,particularlytowitchesbroom

ProjectResults
AhighlysaturatedmapwasdevelopedforvarietySca6xICSandsaturatedmapswereconstructedforvarietiesCABxICS
39andCAB214xICS39.Molecularmarkersrelatedtogeneresistanttowitchesbroomwereidentified.QTLswithimportant
agronomictraitswereidentified.About1300cocoatreesfromrecurrentselectionwereproducedandtransferredtothefield.
Backcrossings in cocoa were conducted to advance backcross generations based on molecular and phenotypic data.
Through germplasm evaluation, molecular data from 800 clones were collected using different types of markers, and the
geneticdiversityofthecloneswasassessed.

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