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J. Glob. Innov. Agric. Soc. Sci., 2015, 3(4): 136-141.

ISSN (Online): 2311-3839; ISSN (Print): 2312-5225


DOI:10.17957/JGIASS/3.4.722
http://www.jgiass.com

SINGLE KERNEL CHARACTERIZATION OF WHEAT VARIETIES IN


RELATION TO MILLING QUALITY
Zafar Iqbal1,*, Imran Pasha2, Muhammad Abrar3, Atta Muhammad Arif4 and Sharoon Masih5
1
Mango Research Institute, Multan, Pakistan, 2 National Institute of Food Science and Technology, University of
Agriculture, Faisalabad, Pakistan, 3 Wheat Research Institute, Ayub Agricultural Research Institute, Faisalabad,
Pakistan, 4Citrus Research Institute, Sargodha, Pakistan, 5 Food Technology Section, Post-Harvest Research Institute,
Ayub Agricultural Research Institute, Faisalabad, Pakistan
*
Corresponding authors e-mail: zafarft@yahoo.co.in

Single kernel characterization system swiftly measures important physical characteristics of wheat grain and provides
forecast for milling quality. Two wheat varieties were tested from SKCS for physical grain parameters. Wheat variety AS
2002 showed higher mean score for kernel weight (32.79mg/kernel), kernel diameter (2.77mm) and seed length (6.47mm)
than Inqulab 91 (30.04mg/kernel, 2.65mm and 5.93mm repectively for kernel weight, diameter and length). Kernel density
(1.61 mg/mm3) and SKCS hardness index (85.65) was comparatively greater in Inqulab 91 than AS 2002 (1.54mg/mm3,
75.51, respectively). AS-2002 exhibited higher break (139.50g/kg), straight grade flour (681g/kg) yield and milling score
(83.28) as compared to Inqulab 91 (107.50g/kg) break, (658.50g/Kg) SGF and (75.19) whereas reduction flour higher in
Inqulab 91 (551g/kg) than AS 02 (541.50g/kg). Mean scores for moisture content, ash content, fat content, protein content,
crude fiber and NFE ranged from 9.52 to 12.10, 0.84 to 1.14, 1.95 to 3.75%, 9.54 to 13.25%, 0.12 to 7.53% and 63.29 to
76.78% respectively in different milled fractions of both wheat varieties. Break flour yield showed significant correlation
with physical grain characteristics like TKW (r=0.97), kernel diameter (r=0.99), milling score (r=0.99), seed length
(r=1.00) and SKCS kernel weight (r=0.92) whereas SKCS hardness (r=-0.60) and kernel density (r=-0.54) were negatively
associated with break flour. Wheat variety with greater TKW, SKCS KW, kernel diameter and seed length yield more
break flour and higher milling score. However, such wheat variety had less SKCS hardness and kernel density.
Keywords: Chemical composition, Mill streams, quality, Single kernel characterization

INTRODUCTION (Pina-D1a/Pinb-D1d) showed the least value for grain


hardness and higher break flour and lesser reduction flour
Traditional flour milling process produces refined flour. yield (Ma et al., 2009). Wheat lines with Pinb-D1b allele
Prime objective of milling is to separate endosperm without showed significant reduction in grain hardness, higher break
contamination of outer parts of grain especially bran and flour yield and milling score and lower flour ash content
germ (Belderok, 2000; Edwards, 2010). During this process, than the Pina-D1b group (Martin et al. 2001, 2008). Pina-
the bran and germ layers of grain are removed in order to D1a/Pinb-D1b genotypes displayed better milling yield than
stabilize the raw material and increase the keeping quality Pina-D1b/Pinb-D1a (Eagles et al., 2006). Endosperm
(Fiste et al., 2014). Dry milling alters the nature and texture affects milling characteristics (Ramseyer et al.,
upsurges the edibility of products obtained from cereals 2011).
(Delcour et al., 2012). Dry milling of wheat results in Milling performance is a complex quality parameter with
production of flour, bran and wheat germ (OECD, 2003). kernel hardness being a highly significant component
Refined flour thus obtained is more acceptable and relishing (Edwards, 2010). Milling efficiency is measured in terms of
food raw material (Delcour et al., 2012). However, with the purity of kernel fractions obtained at each step during
increase in palatability, the nutritive value of refined flour milling (OECD, 2003). Commercial flour mills obtain
decreases (Delcour and Hoseney, 2010). Knowledge of refined flour as 80% of wheat kernel while remaining 20%
kernel parameters can help in improving milling yields is utilized for animal feed and dietary fiber for human foods
(Edwards, 2010). Milling behavior is predicted on the basis (Orth and Shellenberger 1988). Wheat varieties possessing
of many factors (Bechtel et al., 2009). Small improvements distinctive bolting and sifting characteristics with typical
in flour yield are possible by manipulation of these factors. flour recovery and ash content exhibit good milling quality
Hardness and softness are the milling characteristics related (Maghirang et al., 2006). Milling score elucidates yield and
to breakage pattern of endosperm (Hrukov and vec, ash content of flour and integrates it with break flour yield
2009). The wheat hardness is strongly associated with the (Morris et al., 2004). Hard red spring (HRS) wheat showed
semolina and flour yields (Hrukov et al. 2008). However, more total flour and milling score than hard red winter
Vignaux et al. (2004) reported hardness as possible cause (HRW) wheat. Moreover, HRS exhibited less break flour
for reduction in flour yield. Soft-textured wheat genotypes than HRW wheat (Maghirang et al., 2006).
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Iqbal, Pasha, Abrar, Arif & Masih

Hardness Index measured from Single Kernel determined from combined yield of both break and
Characterization System (SKCS) is an arbitrary scale. It reductions flours divided by total weight of all products,
shows a histogram of the distribution of the hardness index times 100 (Morris et al., 2004). All tests were conducted on
values for 300 grains (Edwards, 2010). SKCS 4100 system straight-grade flour. Milling score was calculated by method
provides rapid measurements of wheat physical properties developed by Martin et al. (2007).
important to miller for direct prediction of processing [{100 - [0.50 (16 - percent temper level) + (80 - flour
quality (Osborne and Anderssen, 2003). SKCS is the most yield) + 50 (Percent flour ash - 0.30)]} 1.117] - 3.675
suitable indicator of milling quality for small sample Statistical analysis: The data obtained for each parameter
quantities (Edwards, 2010). Value of hardness index in were subjected to statistical analysis to work out effect of
SKCS is outcome of deformation curve resulting from force wheat varieties on SKCS parameters, milling fractions yield
applied for grinding of individual kernels. Wheat grain and chemical composition by using completely randomized
moisture, kernel size and kernel weight are main factors design and mean values were compared according to
affecting crushing force (Martin et al., 1993). Kernel Duncans Multiple Range test (Steel et al., 1997).
hardness in wheat can be quantified by either NIR, SKCS
hardness index and break flour yield (Morris et al., 1999). RESULTS AND DISCUSSION
Characteristics of wheat kernel are decisive factor in both
the milling quality and final end-use of food wheat SKCS characteristics: Wheat variety AS 2002 with lower
(Hrukov and vec, 2009). Test weight measures bulk hardness index (75.51) represented higher break flour yield
density of grain, envisages the grain size and used as rough than Inqulab 91 having greater score for hardness index
indicator of flour yield potential of wheat varieties (85.65). SKCS hardness index showed insignificant
(Edwards, 2010; Khan et al., 2009). Higher sizing yield is relationship with break flour yield and explained only 27.90
favorable for a high yield of low ash flour (Li and Posner, percent variation in break flour yield. In regression analysis
1989). The 1000 kernel weight is a useful tool for the SKCS hardness could not provide valuable prediction of
assessment of the potential milling yield. The kernel size break flour yield, however Hogg et al., (2005) revealed that
contributes directly towards the improvement of grain as SKCS kernel hardness might be a larger factor for
well as milling yield. The wheat varieties with more grain predicting break flour. Ma et al. (2009) recorded hardness
weight showed better milling yield (Khan et al., 2009). index 67.9 to 75.5 in seven near isogenic lines of wheat
Flour milling alters the microstructure of the intact while 6.4 to 73.7 in Hi-line Hard red spring wheat and
endosperm tissue (Souza et al., 2011). Wheat producing transgenic isolines (Martin et al., 2008). Based on SKCS
flour with low ash at high extraction rates possess good hardness classification, both wheat varieties in current
milling quality (Posner and Hibbs 1997). Ash content studies are of medium hard type. Hard wheats have SKCS
indicates milling performance by indirectly revealing the hardness scores greater than 50 while soft wheats have
amount of bran contamination. Ash can affect color, scores less than 50 (Edwards, 2010). Wheat variety AS-
imparting a darker color to finished products (Keran et al., 2002 having less mass to diameter3 ratio (1.54mg/mm3)
2009). Current study was planned to investigate milling exhibited significant higher break flour yield than Inqulab
performance of wheat varieties and its relation with physical 91 (1.61 mg/mm3). Mean for kernel density in current
characteristics of grain and chemical composition of flour. studies is lower than Souza et al., (2011) who reported
SKCS mass/dia3 vary from 1.88 to 2.39mg/mm3. Ratio of
MATERIALS AND METHODS mass to diameter3 (kernel density) alone elucidated over
50% variation in break flour yield and its value in SKCS
Wheat Milling: Two commercially available wheat varieties forecasts break flour yield (Campbell et al 2007).
i.e. Inqulab-91 and AS-2002 were used as medium of Mean value for kernel weight was significantly higher in
experiment. Perten SKCS 4100 (Perten Instruments, wheat variety AS-2002 (32.79mg/kernel) as compared to
Springfield, IL) was used to measure single kernel grain Inqulab 91 (30.04mg/kernel). SKCS kernel weight ranged
hardness, moisture content and diameter according to from 33.6 to 35.4mg/kernel in Hi-line Hard red spring wheat
Approved Method 55-31 (AACC, 2002) on 300-kernels as and transgenic isolines (Martin et al., 2008) and 34.9-
described by Ma et al,. (2009). Clean wheat grains were 35.3mm (Martin et al., 2007). Souza et al. (2011) recoded
tempered at 15.50% moisture content and kept in plastic mean for SKCS kernel weight in range of 29.1mm to
containers at room temperature for 24 hours. Water quantity 37.4mm in different wheat varieties. Diameter of single
was calculated by AACC (2000) method No. 26-95. Wheat kernel measured from SKCS was considerably higher for
was milled through Brabender Quadrumate Senior Mill AS-2002 (2.77mm) than Inqulab 91 (2.65mm). Martin et al.
(C.W. Brabender Instruments, Inc.) following AACC (2000) (2007) reported SKCS kernel diameter with lower mean
method No. 26-95 to obtain different milling fractions i.e. (2.41-2.45mm) than current studies which might be due to
break, reduction flour, bran and shorts. Percent yield of different genetic make up of wheat varieties. Wheat variety
break, reduction flour, bran and shorts were calculated AS 2002 exhibited significant higher seed length (6.47mm)
based on weight recovered of each product divided by total than Inqulab 91 (5.93mm). Mean values for seed length in
material recovered according to AACC (2000) method No. present investigation are in accordance with findings (5.54-
26-21A. Percent recovery of straight grade flour was 6.29mm) of Souza et al., (2011). Seed length, kernel

137
Varieties in relation to milling quality

diameter and kernel weight illustrated inverse relationship than Souza et al. (2011) who recorded break flour yield
with break flour yield. The results of current studies are from 20.3 to 25.5g/100g in soft red winter and soft white
contradictory to those reported by Souza et al., (2011). This winter wheats. Hard NILs yield more flour at break stage
might me due to different wheat varieties,tempering and than reduction stage (Greffeuille et al., 2006). The
milling conditions and different mill type as quadrumate difference might be due difference in wheat varieties and
senior is used in current in opposition to Malto Miag in their type of mill or tempering conditions. Mean score for
studies. Inverse relationship between flour protein contents reduction flour in current studies is higher than Ma et al.
and kernel weights or sizes (Park and Chang, 2007). (2009) (61.0 to 63.2%) and Randhawa et al. (2002) (40.88-
54.21%). This migh be due to different wheat varieties,
Milling fractions yield: Wheat varieties showed significant tempering moisture content and mill type and settings.
impact on flour yield. Break flour yield was significantly Means for SGF in current investigation are in line with
higher for AS 02 (139.50g/kg) as compared to Inqulab 91 Randhawa et al. (2002) SGF in range of 52.79 to 70.00%
(107.50g/kg) while it was reverse in case of reduction flour and Morris et al., (2004) 67.4 to 70.00% while lower than
(Fig. 1) i.e. higher in Inqulab 91 (551g/kg) than AS 02 Souza et al., (2011) who recorded straight grade flour yield
(541.50g/kg). Straight grade flour yield for was higher for from 71.6 to 76.5g/100g in soft red winter and soft white
AS 02 (681g/kg) as compared to Inqulab 91 (658.50g/Kg) winter wheats. The difference is due to different type of mill
which might be due to difference in tempering requirements used. Maghirang et al. (2006) recorded significantly higher
for hard and soft wheats. Martin et al., (2007) reported flour yield for HRS wheat (67.0%) than to HRW wheat
higher break (430g/Kg) and total flour yield (674g/Kg) in (65.9%). Higher flour yields may result from kernels with
soft wheat as compared to hard wheat varieties with higher endosperm weight (Edwards, 2010). Larger wheat
387g/Kg break flour and 661g/Kg total flour yield. Mean kernels produce higher break releases (Li and Posner, 1987).
values for break in our studies are similar to Anjum et al. Soft wheat gives almost the same percentage of break and
(1993) (65.7 to 70.00%), Ma et al. (2009) (12.4 to 15.5%) reduction flour whereas break flour forms only quarter of
and Randhawa et al. (2002) (11.41 to 19.72%) while lower the reduction flours yield in hard wheat (Edwards, 2010).

Table 1. Milling yield of different flour fractions of wheat varieties


Wheat Varieties Break Flour (%) Reduction Flour (%) SGF (%) Bran (%) Shorts (%) Milling score
Inqulab-91 10.750.80b 55.100.42a 65.850.57b 33.491.18a 5.620.24a 75.191.24b
AS 2002 13.950.80a 54.150.42a 68.100.57a 28.451.18b 4.640.24b 83.281.24a
LSD (0.05) 2.27 2.27 1.70 2.27 0.57 2.27
SD 1.97 1.04 1.40 2.90 0.58 3.04

Table 2. SKCS quality parameters of wheat varieties


Wheat Moisture Kernel SKCS Kernel SKCS Seed Kernel density
varieties content (%) hardness index weight (mg/kernel) Diameter (mm) length (mm) mass/dia3 (mg/mm3)
Inqulab 91 11.310.40a 85.652.52a 30.040.63b 2.650.03b 5.930.13b 1.610.02a
AS-2002 11.980.40a 75.512.52b 32.790.63a 2.770.03a 6.470.13a 1.540.02b
LSD (0.05) 2.27 6.8 0.9 0.11 0.34 0.06
SD 0.27 13.49 7.51 0.3 0.32 0.04

Table 3. Chemical characteristics of mill streams of wheat variety AS 2002


Moisture content Ash content Fat content Protein content Fiber content NFE content
Milling Fractions
(%) (%) (%) (%) (%) (%)
Break Flour 10.900.20ab 0.840.06b 2.960.19ab 10.570.32c 0.120.77e 74.621.21ab
Red. Flour 10.300.20bc 0.570.06c 2.690.19bc 9.540.32d 0.150.77d 76.781.21a
SGF 9.520.20c 0.500.06c 1.950.19c 11.460.32b 0.140.77c 76.411.21a
Bran 11.350.20a 1.050.06a 2.650.19bc 12.540.32a 7.530.77a 64.891.21c
Shorts 11.000.20ab 0.600.06c 3.750.19a 9.570.32d 2.840.77b 72.261.21b
LSD (0.05) 0.91 0.18 0.91 0.64 0.02 2.66
SD 0.79 0.23 0.73 1.23 2.98 4.67

Table 4. Chemical characteristics of mill streams of wheat variety Inqulab 91


Moisture content Ash content Fat content Protein content Fiber content NFE content
Milling Fractions
(%) (%) (%) (%) (%) (%)
Break Flour 11.750.18ab 0.920.05b 3.330.13b 11.130.35c 0.120.75d 72.771.11b
Red. Flour 11.600.18b 0.690.05c 2.850.13c 9.980.35d 0.140.75c 74.751.11a
SGF 10.230.18c 0.600.05c 2.960.13bc 12.450.35b 0.130.75cd 73.641.11ab
Bran 12.100.18a 1.140.05a 2.880.13bc 13.250.35a 7.350.75a 63.291.11d
Shorts 11.650.18ab 0.680.05c 4.000.13a 10.110.35d 2.610.75b 70.961.11c
LSD (0.05) 0.45 0.14 0.45 0.64 0.02 1.70
SD 0.70 0.21 0.50 1.36 2.91 4.30

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Iqbal, Pasha, Abrar, Arif & Masih

Table 5. Correlation coefficients physico-chemical and milling characteristics of wheat varieties


Break NIR Red. SKCS K. SKCS
Parameters PSI SGF TKW TW Ash KD MS SL
Flour hard Flour hard Den. KW
NIR Hard 0.55
PSI 0.53 1.00
Red. Flour -0.06 0.81 0.81
SGF 1.00 0.57 0.56 -0.03
TKW 0.97 0.34 0.33 -0.28 0.97
TW 0.54 1.00 1.00 0.81 0.56 0.34
Ash -0.40 0.55 0.56 0.94 -0.38 -0.60 0.55
KD 0.99 0.65 0.64 0.07 1.00 0.94 0.64 -0.28
SKCS Hard -0.60 0.34 0.35 0.83 -0.58 -0.77 0.34 0.97 -0.50
Ker. Den. -0.54 0.41 0.42 0.87 -0.52 -0.72 0.41 0.99 -0.43 1.00
MS 0.99 0.39 0.38 -0.23 0.98 1.00 0.39 -0.55 0.96 -0.73 -0.68
SL 1.00 0.51 0.49 -0.10 1.00 0.98 0.50 -0.44 0.99 -0.64 -0.58 0.99
SKCS KW 0.97 0.32 0.30 -0.30 0.96 1.00 0.31 -0.62 0.93 -0.78 -0.73 0.99 0.98
Protein -0.08 0.79 0.80 1.00 -0.06 -0.31 0.79 0.95 0.04 0.84 0.88 -0.25 -0.13 -0.33
NIR Hard= NIR hardness; Red. Flour=Reduction flour PSI=Particle Size Index; SGF=Straight grade flour TKW=Thousand kernel weight;
TW=Test Weight; KD=Kernel Diameter; SKCS Hard. =SKCS hardness, K. Den. =Kernel Density; MS=Milling Score; SL=Seed length;
SKCS KW= SKCS kernel weight

Wheat variety Inqulab 91 (5.62%) showed higher mean for bran. AS 2002 Milled fractions revealed higher mean for
shorts than AS 2002 (4.64%). Randhawa et al. (2002) fiber and NFE contents while means for all other chemical
recorded similar yield of shorts (3.01 to 5.89%). Mean value constituents were higher in mill streams of Inqulab 91. The
for bran was significantly higher in Inqulab 91 as compared results for chemical composition of milling fractions of
to AS 2002. The amount of bran from Quadrumate senior wheat varieties in current are indistinguishable to findings
mill ranged from 28.48 to 47.45% (Randhawa et al. 2002). reported by Saeed et al., (2011) and Akhtar et al., (2005).
Mean value for milling score was higher for wheat variety
AS 2002 (83.28) as compared to Inqulab 91 (75.19). Martin Correlation studies: Kernel diameter was significantly
et al., (2007) reported similar results for hard PinA-D1b associated with break flour (r=0.99), SGF (r=1.00), milling
(80.00) and soft PinB-D1b (82.40) wheats. Hard wheats score (r=0.96), seed length (r=0.99), SKCS KW (r=0.93)
exhibit more ash content and less flour yield resulting and protein (r=0.93). SKCS kernel density showed
decrease in milling score than soft wheats. Morris et al. significant positive relationship with reduction flour
(2004) recorded 84.3 to 85.8 milling score in soft wheat (r=0.87), ash content (r=0.99) and SKCS hardness (r=1.00)
varieties which support the findings of current studies. while negative correlation with break flour (r=-0.54), SGF
(r=-0.52) and TKW (r=-0.72) showing that variety with high
Chemical composition of milling fractions: Mean value kernel density has less break flour yield, more reduction
for moisture content ranged from 9.52 to 12.10 in different flour with high ash content. Wheat variety with higher
milling fraction of wheat varieties. Maximum mean for TKW, seed length and kernel weight whereas less density
moisture was recorded in bran (12.10, 11.35) while and hardness yield more flour as evident from direct
minimum in SGF (10.23, 9.52) respectively for Inqulab 91 correlation of milling score with TKW (r=1.00), seed length
and AS-2002. Mean for ash content varied between 1.05 to (r=0.99) and SKCS KW (r=0.99) while inverse relationship
1.14, 0.50 to 0.68, 0.84-0.92, 0.57 to 0.69, 0.50-0.60% with kernel density (r=-0.73) and SKCS hardness (r=-0.68).
respectively in bran, shorts, break, reduction and straight Greater seed length provides assurance for higher flour
grade flours which are well supported by findings of Saeed recovery. Seed length depicted strong positive association
et al. (2011). Fat content of different milled fraction assorted with break flour (r=1.00), SGF (r=1.00) and TKW (r=0.99)
between 3.75-4.00, 2.65 to 2.88, 2.96-3.33, 2.69-2.85, 1.95- while kernel density (r=-0.58) and SKCS hardness (r=-0.64)
2.96% respectively in shorts, bran, break, reduction and was negatively related with seed length.
straight grade flours. Shorts exhibit higher mean for fat Significant correlation of break flour yield with physical
content while lowest value recorded in SGF. Statistical grain characteristics like TKW (r=0.97), kernel diameter
mean values for protein concentration of wheat varieties (r=0.99), milling score (r=0.99), seed length (r=1.00) and
ranged between 12.54-13.25, 9.57-10.11, 10.57-11.13, 9.54- SKCS kernel weight (r=0.92) is indication of higher break
9.98, and 11.45-12.46% respectively for bran, shorts, break, flour yield in wheat variety with soft texture as compared to
reduction and straight grade flours. The range of means for hard wheat. Whereas SKCS hardness (r=-0.60) and kernel
crude fiber content varied between 7.35 to 7.53, 2.61 to density (r=-0.54) were negatively associated with break
2.84, 0.12, 0.14-0.15, 0.13-0.14 respectively in bran, shorts, flour recovery. SKCS kernel weight exhibited direct
break, reduction and SGF flours. NFE content in different relationship with TKW (r=1.00), break flour (r-0.97), kernel
milling fractions differed from 74.75 to 76.78, 73.64 to diameter (r=0.93) and seed length (r=0.98) whereas it was
76.41, 72.77 to 74.62, 70.96 to 72.26 and 63.29 to 64.89% negatively associated with ash content (r=-o.62), SKCS
respectively for reduction, SGF, break flour, shorts and hardness (r=-0.78) and kernel density (r=-0.73). Seed length
139
Varieties in relation to milling quality

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with flour yield (Edwards et al., 2008). SKCS 4100 HI Contributions of glutenin and puroindoline genes to
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CONCLUSIONS
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Edwards, M.A. 2010. Morphological features of wheat grain
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Southern Cross University, Lismore, NSW.
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