Alexandre Monteiro

1. Recupere e alinhe as seqncias do citocromo b de cavalo, baleia e canguru.

(a) Compare o grau de similaridade para cada par de seqncias com o resultado da
comparao das seqncias da ribonuclease pancretica destas espcies no Estudo de
Caso 1.2. As concluses tomadas com base na anlise das seqncias do citocromo b
so consistentes com as concluses tomadas com base na anlise da ribonuclease
pancretica?

>sp|P00674|RNAS1_HORSE Ribonuclease pancreatic OS=Equus caballus

GN=RNASE1 PE=1 SV=1
KESPAMKFERQHMDSGSTSSSNPTYCNQMMKRRNMTQGWCKPVNTFVHEPLADVQAICLQ
KNITCKNGQSNCYQSSSSMHITDCRLTSGSKYPNCAYQTSQKERHIIVACEGNPYVPVHF
DASVEVST

>sp|P00673|RNAS1_BALAC Ribonuclease pancreatic OS=Balaenoptera

acutorostrata GN=RNASE1 PE=1 SV=1
RESPAMKFQRQHMDSGNSPGNNPNYCNQMMMRRKMTQGRCKPVNTFVHESLEDVKAVCSQ
KNVLCKNGRTNCYESNSTMHITDCRQTGSSKYPNCAYKTSQKEKHIIVACEGNPYVPVHF
DNSV

>sp|P00686|RNAS1_MACRU Ribonuclease pancreatic OS=Macropus rufus

GN=RNASE1 PE=1 SV=1
ETPAEKFQRQHMDTEHSTASSSNYCNLMMKARDMTSGRCKPLNTFIHEPKSVVDAVCHQE
NVTCKNGRTNCYKSNSRLSITNCRQTGASKYPNCQYETSNLNKQIIVACEGQYVPVHFDA
YV

CLUSTAL 2.1 Multiple Sequence Alignments

Sequence type explicitly set to Protein

Sequence format is Pearson
Sequence 1: RNAS1_HORSE 128 aa
Sequence 2: RNAS1_BALAC 124 aa
Sequence 3: RNAS1_MACRU 122 aa
Start of Pairwise alignments
Aligning...

Sequences (1:2) Aligned. Score: 76

Sequences (1:3) Aligned. Score: 61
Sequences (2:3) Aligned. Score: 67
Guide tree file created: [clustalw2-I20111202-201836-0429-18491404-
pg.dnd]

There are 2 groups

Start of Multiple Alignment

Aligning...
Group 1: Sequences: 2 Score:2496
Group 2: Sequences: 3 Score:2251
Alignment Score 1672

CLUSTAL-Alignment file created [clustalw2-I20111202-201836-0429-

18491404-pg.aln]
>gi|328909490|gb|HQ890258.1| Equus caballus clone Steinbach10_f03
succinate dehydrogenase cytochrome b560 subunit mitochondrial-like
protein (SDHC) mRNA, partial cds
GCCAAAGAAGAGATGGAAAGATTCTGGAATAAGAACATTGGTTCAAACCGTCCTATGTCTCCCCATATCA
CTATCTACAGTTGGTCTCTTCCTATGGTGATGTCCATTTGCCACCGTGGAACTGGTATTGCCTTGAGTGC
AGGGGTCTCTCTTTTTGGCTTGTCCGCCCTACTGGTCCCTGGGAACTTTGAGTCTCATTTGGAACTTGTA
AAGTCCCTGTGTCTGGGGCCAGCACTGATCCACACAGCCAAGTTTGCACTTGTCTTCCCTCTCATGTATC
ACACCTGGAATGGGATCCGACACTTGATATGGGACCTAGGAAAAGGTCTGAAGATTCCCCAGCTATACCA
GTCTGGAGTGGTTGTCTTGGTTCTTACTGTGTTGTCCTCTGTAGGGCTGGCAGCCATGTGAAGAGCTGAA
GTTTCCAACATCATCTTCCTACAAATGATTATATTGACCTGTCTTCCTGTTTGTCACTCTTATCTCCAGC
CAGGACAAAGTTCTCCTGATTTGTTTAGTCCCTTTTGTGCTTGCACATCCCCTTGAGCTCAGCAATAGTG
TACCATATAGAAACATAGTAGTGGAAAAGGGTCCACTTTTCCCCTTGTTTCTAAAGATGGAATGACTGCA
AAACTCCCTTTTCCTGTCCCCAGCTTGCAGCCTACTCTGGGCCTAGGACCCTTATTCTGTCTCCATATTG
GGCCTTGATTTGTGCTGAGGGTCAGCTTTTGGCTCCTTCTTCCTGAGACAGTGGAAACAATGCCAGCTCT
GTGGCCTCTGCCATGGGGACTGGGAGTGGGGCTTCGGGTGCCACTGCCTGTGGGTTGCCGGTTTAAAGGA
CAGTTCCGTTCATTGGTCAGAGCCCAGGGTCTTTAGCACCCACGCAGTATGACTGAAGAGAGGGTGGGGT
TGGGAGGGGAATTACCCTGTCCCAGCTAGAGGGAGATAAAGAGAGTGAGTTGGCTCTTGCAGCAGTGCTT
TGGGA

>gi|294484462|gb|HM034299.1| Balaenoptera acutorostrata isolate

K09.011 cytochrome b (cytb) gene, partial cds; mitochondrial
CACACCCACTAATAAAAATTATCAACGACGCATTCGTCGATCTACCCACCCCATCAAACATCTCCTCATG
ATGAAACTTTGGCTCTCTACTCGGCCTCTGCTTAATTGTACAAATCCTAACAGGTCTATTCCTAGCAATA
CACTACACACCAGACACAACAACCGCCTTCTCATCAGTTACACACATTTGCCGAGACGTAAACTACGGTT
GAATCATCCGATATCTACATGCAAATGGAGCCTCTATATTCTTCATCTGCCTTTACGCCCACATAGGGCG
AGGCCTATACTATGGCTCCCACGCCTTCCGAGAAACATGGAATATCGGAGTCATCCTACTATTCACAGTT
ATAGCTACCGCATTCGTAGGCTACGTCCTACCC

>gi|306441059|gb|HQ122581.1| Macropus rufus cytochrome b (cytb) gene,

partial cds; mitochondrial
CTCATATCTCTACAAAGAAACATGAAATATCGGAGTAATCCTCCTACTAACCGTCATAGCAACAGCTTTC
GTAGGCTATGTCCTACCGTGAGGACAAATATCATTCTGAGGGGCTACCGTAATTACCAATCTCCTATCCG
CTATCCCCTACATCGGTACAACACTAGTAGAATGAATCTGAGGTGGATTCTCCGTAGACAAAGCCACCCT
TACACGCTTTTTTGCCTTCCACTTTATCTTACCATTTATTATTACAGCCTTAGTACTAGTCCACCTCCTA
TTCCTACACGAAACAGGCTCCAACAACCCATCAGGAATCAACCCCGACTCAGACAAAATCCCATTCCACC
CATATTATACAATCAAAGATGCACTTGGCTTCATACTTATACTCCTCATCCTACTCACATTAGCACTATT
CTCACCCGACATACTAGGAGACCCAGATAACTTCTCCCCCGCCAACCCACTGAACACTCCATCGCATATT
AAACCAGAATGATATTTCCTATTTGCA

CLUSTAL 2.1 Multiple Sequence Alignments

Sequence type explicitly set to Protein

Sequence format is Pearson
Sequence 1: Equus_caballus 985 aa
Sequence 2: Balaenoptera_acutorostrata 383 aa
Sequence 3: Macropus_rufus 517 aa
Start of Pairwise alignments
Aligning...

Sequences (1:2) Aligned. Score: 48

Sequences (1:3) Aligned. Score: 47
Sequences (2:3) Aligned. Score: 45
Guide tree file created: [clustalw2-I20111202-201648-0148-94442549-
pg.dnd]

There are 2 groups

Start of Multiple Alignment

Aligning...
Group 1: Sequences: 2 Score:6294
Group 2: Sequences: 3 Score:6118
Alignment Score 3553

CLUSTAL-Alignment file created [clustalw2-I20111202-201648-0148-

94442549-pg.aln]

Em ambos os alinhamentos as similaridades foram semelhantes entre si, apesar de

divergirem quanto protena.

(b) Compare a similaridade relativa destas seqncias com os resultados da comparao

das seqncias da ribonuclease pancretica destas espcies no Estudo de Caso 1.2. As
concluses tomadas com base na anlise das seqncias do citocromo b so consistentes
com as concluses tomadas com base na anlise da ribonuclease pancretica?

Relativamente as sequencias apresentam um mesmo grau de similaridades entre si,

porm se compararmos os scores das duas protenas podemos observar uma diferena
considervel.

2. Observamos que as seqncias de aminocidos do citocromo b de elefantes e de

mamute so bastante similares. Uma hiptese para explicar esta observao que o
citocromo b funcional requer tantos resduos conservados que os citocromos b de todos
os animais so similares uns aos outros, assim como as protenas dos elefantes e
mamute. Teste esta hiptese obtendo as seqncias do citocromo b de outras espcies
pouco relacionadas, ou distantes, so to similares seqncia dos elefantes quanto o
so as seqncias de elefantes e mamute.

>gi|33326105|gb|AF519689.1| Betta splendens isolate B194 cytochrome b

(cytb) gene, complete cds; and tRNA-Thr gene, partial sequence;
mitochondrial
ATGACCCACCAACGTAAAAATCACCCACTACTGAAAATCGCCAACAACGCACTAATTGACCTCCCCGCCC
CAGCCAGCATTTCCGCCTGATGAAATTTTGGGTCATTACTAGGACTATGTCTTATTATTCAGATCTTAAC
AGGCCTTTTCCTTGCTATACACTATACCTCTGATATCACTATAGCCTTTTCATCAATTACCCACATCTGC
CGTGATGTAAACTATGGCTGATTCATTCGAAACATACATGCCAATGGGGCATCTTTCTTTTTTATCTGTA
TCTATTTACATATTGGTCGAGGATTATATTATGGTTCATACACAAATAAAGAAGTATGAAACGTAGGTGT
TTTACTACTCCTACTCACCATGATAACAGCCTTCGTAGGATATGTTTTGCCTTGAGGACAAATATCTTTT
TGAGGTGCTACTGTTATTACCAACTTACTATCTGCCATCCCCTACGTAGGGGACACCCTCGTCCAATGAA
TTTGAGGGGGATTTTCAGTTAACAACGCCACCCTAACACGATTCTTCGCCTTTCATCTTTTATTCCCCTT
TATTATCGCAGCTATAACAATAATCCACTTACTTTTTTTACATGAAACAGGTTCAAATAACCCAATAGGT
TTAAACTCTAACGTAGATAAAGTCACCTTTCACCCCTACTTCTCATATAAAGATTTGTTAGGATTTACAA
TTCTATTAATTACACTACTCTCCTTGTCACTATTTTCACCAAATTTACTAGGTGACCCAGACGATTTTAC
CCCTGCCAACCCCTTAGTTACCCCACCACACATTAAACCTGAATGATATTTCTTATTCGCTTATGCCATT
CTTCGCTCAATCCCTAATAAACTAGGAGGAGTTCTAGCCCTGTTAGCCTCAATCTTAGTCCTTATATTAG
TACCAATTTTACACACATCTAAACAACGAAGCCTAACTTTCCGTCCCCTTTCACAATTTTTATTTTGAAT
TCTAATTGCAGATGTAATAATTCTCACTTGAATTGGAGGAATGCCAGTAGAAAACCCCTACATCATTATT
GGACAAATAGCATCTTTCCTTTACTTTTCTCTATTTCTAGTACTTATACCTATAGCAGGGCTTCTAGAAG
ATGAAATTGTTTTCAAGCACATTACCTGCCCTAGTAGCTCAGCGCC

>gi|3172442|gb|AF064587.1| Canis familiaris breed shepherd cytochrome

b gene, mitochondrial gene encoding mitochondrial protein, partial cds
CCGTCTAACATCTCTGCTTGATGGAACTTCGGATCCTTACTAGGAGTATGCTTGATTCTACAGATTCTAA
CAGGTTTATTCTTAGCTATGCACTATACATCGGACACAGCCACAGCTTTTTCATCAGTCACCCACATCTG
CCGAGACGTTAACTACGGCTGAATTATCCGCTATATGCACGCAAATGGCGCTTCCATATTCTTTATCTGC
CTATTCCTACATGTAGGACGAGGCCTATATTACGGATCCTATGTATTCATAGAAACATGAAACATTGGAA
TTGTACTATTATTCGCAACCATAGCCACAGCATTCATGGGCTATGTACTACCATGAGGACAAATATCATT
TTGAGGAGCAACTGTAATCACTAATCTTCT

>gi|57235285|gb|AY704849.1| Crotalus molossus nigrescens isolate CMN4

cytochrome b (cytb) gene, partial cds; mitochondrial
ATAATCCAAATCATCACCGGCTTCTTCTTGGCAATTCACTACACAGCCAACATTAATATAGCCTTTTCCT
CCATTGTACACATCTCCCGGGACGTCCCCTACGGCTGAATTATACAAAACACACACGCCATCGGCGCATC
TCTATTTTTCATTTGCATCTACATCCACATCGCACGAGGCATCTACTACGGCTCCTACCTAAATAAAGAA
GTTTGACTATCAGGCACCACCCTACTAATTATCCTAATAGCCACCGCCTTCTTTGGTTACGTACTACCAT
GAGGTCAAATATCATTCTGAGCAGCAACAGTAATCACTAATCTATTAACTGCCATCCCGTACCTAGGAAC
AACACTCACCACATGACTTTGAGGAGGCTTCGCAATCAACGACCCAACATTAACTCGATTCTTCGCCCTT
CACTTTATCCTTCCATTCGCTATTATCTCCATTTCCTCACTCCACATCCTTCTCCTACACAACGAAGGCT
CCAACAACCCACTAGGTACAAATTCGGACATCGATAAAATCCCATTTCACCCCTACCACTCATACAAAGA
CACTCTTATACTTACAACAATAATCACCCTACTGTTTATTACCCTATCTCTTTACCCCGATATACTCAAC
GACCCAGAAAACTTCTCAAAAGCTAACCCCTTGGTCACACCACAA

CLUSTAL 2.1 Multiple Sequence Alignments

Sequence type explicitly set to Protein

Sequence format is Pearson
Sequence 1: Betta_splendens 1166 aa
Sequence 2: Canis_familiaris 380 aa
Sequence 3: Crotalus_molossus 675 aa
Start of Pairwise alignments
Aligning...

Sequences (1:2) Aligned. Score: 70

Sequences (1:3) Aligned. Score: 66
Sequences (2:3) Aligned. Score: 57
Guide tree file created: [clustalw2-I20111202-203744-0578-32565922-
pg.dnd]

There are 2 groups

Start of Multiple Alignment

Aligning...
Group 1: Sequences: 2 Score:7063
Group 2: Sequences: 3 Score:8259
Alignment Score 6010

CLUSTAL-Alignment file created [clustalw2-I20111202-203744-0578-

32565922-pg.aln]

Apesar de distantes filogeneticamente, as sequencias apresentam uma relativa

similaridsade, por se tratar de uma estrutura comum a todos os animais analisados.
3. Recupere e alinhe as seqncias do citocromo c de humanos, da cobra cascavel e do
lagarto-monitor. Qual par de seqncias parece estar mais proximamente relacionado?
Isso o surpreende? Por qu? Ou por que no?

>gi|188536035|ref|NM_024843.3| Homo sapiens cytochrome b reductase 1

(CYBRD1), transcript variant 1, mRNA
GAGTTGGGGCCAGCTCCCCACCCCCAAGAGGCCCCACATTCCGGGCCAGCAGCCCAGAAAGTCCCTCCCC
GCAGGCGGAGACAGCCCCAAGAAGTCGACGCCCCGGTCCCGCCGCCCGGCCACTACCCAGAGGGCTGCCG
CCGCCTCTCCAAGTTCTTGTGGCCCCCGCGGTGCGGAGTATGGGGCGCTGATGGCCATGGAGGGCTACTG
GCGCTTCCTGGCGCTGCTGGGGTCGGCACTGCTCGTCGGCTTCCTGTCGGTGATCTTCGCCCTCGTCTGG
GTCCTCCACTACCGAGAGGGGCTTGGCTGGGATGGGAGCGCACTAGAGTTTAACTGGCACCCAGTGCTCA
TGGTCACCGGCTTCGTCTTCATCCAGGGCATCGCCATCATCGTCTACAGACTGCCGTGGACCTGGAAATG
CAGCAAGCTCCTGATGAAATCCATCCATGCAGGGTTAAATGCAGTTGCTGCCATTCTTGCAATTATCTCT
GTGGTGGCCGTGTTTGAGAACCACAATGTTAACAATATAGCCAATATGTACAGTCTGCACAGCTGGGTTG
GACTGATAGCTGTCATATGCTATTTGTTACAGCTTCTTTCAGGTTTTTCAGTCTTTCTGCTTCCATGGGC
TCCGCTTTCTCTCCGAGCATTTCTCATGCCCATACATGTTTATTCTGGAATTGTCATCTTTGGAACAGTG
ATTGCAACAGCACTTATGGGATTGACAGAGAAACTGATTTTTTCCCTGAGAGATCCTGCATACAGTACAT
TCCCGCCAGAAGGTGTTTTCGTAAATACGCTTGGCCTTCTGATCCTGGTGTTCGGGGCCCTCATTTTTTG
GATAGTCACCAGACCGCAATGGAAACGTCCTAAGGAGCCAAATTCTACCATTCTTCATCCAAATGGAGGC
ACTGAACAGGGAGCAAGAGGTTCCATGCCAGCCTACTCTGGCAACAACATGGACAAATCAGATTCAGAGT
TAAACAGTGAAGTAGCAGCAAGGAAAAGAAACTTAGCTCTGGATGAGGCTGGGCAGAGATCTACCATGTA
AAATGTTGTAGAGATAGAGCCATATAACGTCACGTTTCAAAACTAGCTCTACAGTTTTGCTTCTCCTATT
AGCCATATGATAATTGGGCTATGTAGTATCAATATTTACTTTAATCACAAAGGATGGTTTCTTGAAATAA
TTTGTATTGATTGAGGCCTATGAACTGACCTGAATTGGAAAGGATGTGATTAATATAAATAATAGCAGAT
ATAAATTGTGGTTATGTTACCTTTATCTTGTTGAGGACCACAACATTAGCACGGTGCCTTGTGCAGAATA
GATACTCAATATGTGAATATGTGTCTACTAGTAGTTAATTGGATAAACTGGCAGCATCCCTGGCCTGTTG
TCATGCAGTCATTTCCTGTTAATTCTGGGAGACAATGATTTCACAACTAGCGGGAAGCAGTCCTAAAAGT
TTAAAATCCGATAAGGAATATCTGGGACAGGGTTTAGATCATGACTCTACACAGATACCATGATGAGAGT
ATATTAAAGAAATTTAGGAAAGCACCTGGTTCCTTTCTCCCCATGCCTGCCTTCTGCTCCCTCCCCAGCT
GGTTTGGGCTCAAATTGTCCCTGGAGACTAGGGTTTATGTTAGGGTATTGATAGATTAGAGCAGGTGGTT
GAAGAGATCTTCTCTGGTCAGACTTGGAAGAATTTCCAAAACTGAAGTTAGCCCCAAGACTTCCCTAGGG
TTGATGTACTTTATGATCCAGATGCTAAACTTCTTAGAATGAAAATATGCTTCAACACTTAAGTAGCATA
CACTGCCCTACAAACCTCAGAGAGCACTTTTCCCCAAGTTCTTGTTTTTATTTTTGAAAGTACTCACACA
GCACTTACTATGCTCCAAACACTCCTCTAAGCACTTTACACATATTAGCTCATTCAGTCCCCAGACAGAC
GGGATGAAGTAGGTATTGTTACTGTTCCCATTTTACAGGTGAGAGATTTGAAGCCTGGGGAGGCTAGTAA
CTCACCCCAAGGTCACACGGCTCATACATGGTGGGACTGAGACTCAGATGCAGGCAGTCTGGCACCTCAG
TCTGGATTCTAACCATTTCACTAAGCTATTTTTGTCTTGTACTACTTTGACCCACCCCTGAATAAACCTC
AATTGCTGGAGTGGGGTGTAGTTATTAAAGGGATGCTTTTTACCTTTTGCTGTTTGCTGTGGCAGATTCC
CCAGATAACCAAGGAAAAGGGGCCACCCATACCTGGAAATAGGCCATAGGGCCCCTACTACTGCCAACAA
GCCATGGCCTACCTTGACACTTGTTTGATCTTAAAATTGTGTCTTGGTAACAAAAGATTTGGACAGGCAT
ATCTGTAGCTTTCAAGTTAATTAATTGCAATATTTTTTTCTTCAGGATTTTAGCTGCTGAACAACTTTCA
GTTTGGAGCTAAAAGAGACCTGTCTCATGGTCTGCCCTTCCCTGGGGCAATAGCTAGGGTCTTTCCTGAT
TTTTATGGAATTTTAGGGGATATTTTGAGCTTTGGGTTCTCAGTAGTGAATTGAGACTTGGAGGTGACTT
TTCATGTTTGGAGTATCATCTCTGTCTGGGATCTGGGCTGACAAATTAAAACCTAGAGTAGTGCTTATGC
TGAAATGATACTTTTCATTTTTTGGTTGATTTTTTTGCCTTCCCTTCAATTTTAAACTGAAGCATTTTAA
TATGGGTAGAAACTCTACACCAAATACACTAAACATTTTGGTGCTTAGTGGATTTCTTTTTAGGTAACTG
GTACTTACTTCCAAAGACTGAATACAAGCCACACTCCATCATATCCCTTAAACTTCATGAAAAACCATTC
AAGATCCCCTTGCTGCAACACTGTTCTCTTCTTCTCTACTAAATTCTATTTCCAAAATTGGTAATAGAGC
CAGAAGGATCCCCAGTACCCAGCCCTCTGCCTGGCACAAAGTGGTAGCACAATTAAATTCAGTATGGGTG
GAGCATGGTACAGTCTTGGTGCCATAGAAGGAGTAGTTGCATAGTCACACATCATTTGATAAGTTGGATG
TTCCATTACATAGAGGAACACAAAATTCCAGGGTTTTTGGAGGAAGGGATTAGATAGTGACTAAGCCGCC
AGAATTGAGGTGGCCATTCCTTTTTGTATAGGCTAAGAAACAGGTTATCAGTGAAAAGTTAATTATGGCT
TTGGCACTAGAATAGCACTGTTGCAAAGTATTTAAGCACCCCCCATCTCAGCCCTTTATTTTATCTTTCA
TGTGGGCTAATGTGAGGATAATCTTACAGATATTATAGGAATTTCTTTTCTATCTTTATGAAAACAACGT
ATATAAAATATATCTAGAAAACCTTTGTTTGAGACTCTTATTTAATGGGCTTTTGATTCTAATGATAATT
GTACCTTTATCTTTCAAAAGCTGATATTTCCTACCTAAGCATCTCCCGAGAAAAATATCTCATTAAAAAG
CCCATAAATAATAGGGGAGAAGAAAGCCTTAGGTATCAATTCCAAAACAGTGATTGAAATTTCCCAAAAT
AATTATGGCTTCTGTCATCTCCAGAGATAATCTGGCTTGGTTTACCCCATAATCTAATTTCAGAAAAGAA
AGCTTTATTTTAACACTCATCTGAATCAACATTAAAGCCTTTTCTCTCAAAGCGTTTATTGAGAAACTCA
AATGAATATACTTTTTGAATTACTGTCATCAAAAGTGTACGGCTTCCTGTGCTGCTTGTGTCAAATGGAA
CCTGCCCTCTAAAGCACTTTCTTTCCTTTACTTGCGTGGTTTCATGTAAGCTGTGCTGTTTAGAAACAAC
ATCTCAGACTTTACAAAGAAATGACAAAGAAGGCAATTGCACTTTTTAAGGGATATCGACAAGCAGTTTC
TGTTTTCTAAAGGACAAAATACAGAGTGTGTGTCATTTTTAATTAGATTCTTTCCCCTGCTGAGTTGGAA
ATTCCAGTGCAGCACTGATTGACCACAGTTGCCAATCTAAAAGCACAAAGACAGAAGTAAAGCTTTATGC
TAATTTTATTTCAATATGATAGAAAATTTATCTTGGTATGTCCTTTTTTAGATAACTCCAGCAGGAAACT
GTAACTGCTATGTCTTTAGGAAAATGTAGAAGAAAGAACATTATTGTTCTTTAATTCCTACAAGGTACTC
GAAAACCTTAAGTGAAAAAGATTTCTATCTTTTTATCTTAGCGCATTTATGGAAAAAATATTAACTATCC
TGAATATTTTATAATTTTGTAGGAAAAATATGCATCTATTTTTTCTTGACTTCTTTTATATAGTAATAAA
AGTTATTTTGGAAGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

>gi|57235285|gb|AY704849.1| Crotalus molossus nigrescens isolate CMN4

cytochrome b (cytb) gene, partial cds; mitochondrial
ATAATCCAAATCATCACCGGCTTCTTCTTGGCAATTCACTACACAGCCAACATTAATATAGCCTTTTCCT
CCATTGTACACATCTCCCGGGACGTCCCCTACGGCTGAATTATACAAAACACACACGCCATCGGCGCATC
TCTATTTTTCATTTGCATCTACATCCACATCGCACGAGGCATCTACTACGGCTCCTACCTAAATAAAGAA
GTTTGACTATCAGGCACCACCCTACTAATTATCCTAATAGCCACCGCCTTCTTTGGTTACGTACTACCAT
GAGGTCAAATATCATTCTGAGCAGCAACAGTAATCACTAATCTATTAACTGCCATCCCGTACCTAGGAAC
AACACTCACCACATGACTTTGAGGAGGCTTCGCAATCAACGACCCAACATTAACTCGATTCTTCGCCCTT
CACTTTATCCTTCCATTCGCTATTATCTCCATTTCCTCACTCCACATCCTTCTCCTACACAACGAAGGCT
CCAACAACCCACTAGGTACAAATTCGGACATCGATAAAATCCCATTTCACCCCTACCACTCATACAAAGA
CACTCTTATACTTACAACAATAATCACCCTACTGTTTATTACCCTATCTCTTTACCCCGATATACTCAAC
GACCCAGAAAACTTCTCAAAAGCTAACCCCTTGGTCACACCACAA

>gi|195947075|dbj|AB301968.1| Varanus exanthematicus mitochondrial

genes for cytochrome b oxidase, tRNA-thr, D-loop region
ATTTACCTTTCAATCCCCAATAAATTAGGAGGTGTTATGGCCCTCCTTGCCTCCATCCTCATTCTCCTAC
TCATTCCATTCTTACACCAATCTAAACAACAAACCATAAACTTTCGTCCCCTGTCTCAACTTTTATTCTG
AACAATCATCACTAACATTTTCATCTTAACATGAATTGGAGGACAGCCCGTCGAAGACCCATTCATCCTC
ATTGGACGCATTTCCTCAACCATTTATTTCTTCCTATTCCTCTCCATACCCCCCATCATAACTATAGAGA
ACAAACTACTTAATTGATAGTCCTAGTAGCTTAATAATAAAGCATTGACCTTGTAAGCCAAAGAATGGGA
CCCCCCCTAGGACATTCCTGCTTTCCCCTACCCAGACGTCCCGAATTTTCCCCTTT

A cascavel mais semelhante ao lagarto, pois evolutivamente so mais prximos, como

j era esperado.

4. Envie as seqncias da ribonuclease pancretica de cavalo, baleia-branca e canguru-

vermelho (Estudo de Caso 1.2) para o servidor de alinhamentos mltiplos T-coffee:
http://ch.embnet.org/software/TCoffee.html. O resultado do alinhamento o mesmo do
resultado mostrado no Estudo de Caso 1.2, gerado pelo CLUSTAL-W? Se no for, qual
a diferena?
> CYB_HORSE Cytochrome b OS=Equus caballus GN=MT-CYB PE=3 SV=1
MTNIRKSHPLIKIINHSFIDLPAPSNISSWWNFGSLLGICLILQILTGLFLAMHYTS
DTT
TAFSSVTHICRDVNYGWIIRYLHANGASMFFICLFIHVGRGLYYGSYTFLETWNI
GIILL
FTVMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTTLVEWIWGGFSVDKA
TLTRFFA
FHFILPFIITALVVVHLLFLHETGSNNPSGIPSDMDKIPFHPYYTIKDILGLLLLILL
LL
TLVLFSPDLLGDPDNYTPANPLSTPPHIKPEWYFLFAYAILRSIPNKLGGVLALIL
SILI
LALIPTLHMSKQRSMMFRPLSQCVFWLLVADLLTLTWIGGQPVEHPYVIIGQLA
SILYFS
LILIFMPLASTIENNLLKW

>CYB_BALAC Cytochrome b OS=Balaenoptera acutorostrata GN=MT-CYB PE=3

SV=1
MTNIRKTHPLMKIINDAFIDLPTPSNISSWWNFGSLLGLCLIVQILTGLFLAMHYT
PDTT
TAFSSVTHICRDVNYGWIIRYLHANGASMFFICLYAHMGAGLYYGSHAFRETW
NIGVILL
FTIMATAFVGYVLPWGQMSFWGATVITNLLSAIPYIGTTLVEWIWGGFSVDKA
TLTRFFA
FHFILPFIILALAIVHLIFLHETGSNNPTGIPSDMDKIPFHPYYTIKDILGALLLILTL
L
ALTLFAPDLLGDPDNYTPANPLSTPAHIKPEWYFLFAYAILRSIPNKLGGVLALL
LSILI
LAFIPMLHTSKQRSMMFRPFSQSLFWVLVADLLTLTWIGGQPVEHPYMIVGQL
ASILYFL
LILVLMPVASLIENKLMKW

>CYB_MACRU Cytochrome b OS=Macropus rufus GN=MT-CYB PE=3 SV=1

MTNLRKTHPLIKIVNHSFIDLPAPSNISAWWNFGSLLGACLIIQILTGLFLAMHYT
ADTL
TAFSSVAHICRDVNYGWLIRNLHANGASMFFMCLFLHVGRGIYYGSYLYKETW
NIGVILL
LTVMATAFVGYVLPWGQMSFWGATVITNLLSAIPYIGTTLVEWIWGGFSVDKA
TLTRFFA
FHFILPFIITALVLVHLLFLHETGSNNPSGINPDSDKIPFHPYYTIKDALGFMLMLL
ILL
TLALFSPDMLGDPDNFSPAKPTEHSSHIKPEWYFLFAYAILRSIPNKLGGVLALL
ASILI
LLIIPLLHTSKQRSLMFRPISQTLFWILTANLITLTWIGGQPVEQPYIIIGQVASISY
FL
LIIVLMPLAGLFENYMLEPKW

MSAThe multiple sequence alignment result as produced by T-coffee.

T-COFFEE, Version_9.01 (2011-10-13 19:18:48 - Revision 1115 -
Build 7)
Cedric Notredame
SCORE=99
*
BAD AVG GOOD
*
CYB_HORSE : 99
CYB_BALAC : 99
CYB_MACRU : 99
cons : 99

CYB_HORSE 1 MTNIRKSHPLIKIINHSFIDLPAPSNISSWWNFGSLLGI 39
CYB_BALAC 1 MTNIRKTHPLMKIINDAFIDLPTPSNISSWWNFGSLLGL 39
CYB_MACRU 1 MTNLRKTHPLIKIVNHSFIDLPAPSNISAWWNFGSLLGA 39

cons 1 ***:**:***:**:*.:*****:*****:********* 39

CYB_HORSE 40 CLILQILTGLFLAMHYTSDTTTAFSSVTHICRDVNYGWI 78
CYB_BALAC 40 CLIVQILTGLFLAMHYTPDTTTAFSSVTHICRDVNYGWI 78
CYB_MACRU 40 CLIIQILTGLFLAMHYTADTLTAFSSVAHICRDVNYGWL 78

cons 40 ***:*************.** ******:**********: 78

CYB_HORSE 79 IRYLHANGASMFFICLFIHVGRGLYYGSYTFLETWNIGI 117

CYB_BALAC 79 IRYLHANGASMFFICLYAHMGAGLYYGSHAFRETWNIGV 117
CYB_MACRU 79 IRNLHANGASMFFMCLFLHVGRGIYYGSYLYKETWNIGV 117

cons 79 ** **********:**: *:* *:****: : ******: 117

CYB_HORSE 118 ILLFTVMATAFMGYVLPWGQMSFWGATVITNLLSAIPYI 156

CYB_BALAC 118 ILLFTIMATAFVGYVLPWGQMSFWGATVITNLLSAIPYI 156
CYB_MACRU 118 ILLLTVMATAFVGYVLPWGQMSFWGATVITNLLSAIPYI 156

cons 118 ***:*:*****:*************************** 156

CYB_HORSE 157 GTTLVEWIWGGFSVDKATLTRFFAFHFILPFIITALVVV 195

CYB_BALAC 157 GTTLVEWIWGGFSVDKATLTRFFAFHFILPFIILALAIV 195
CYB_MACRU 157 GTTLVEWIWGGFSVDKATLTRFFAFHFILPFIITALVLV 195

cons 157 ********************************* **.:* 195

CYB_HORSE 196 HLLFLHETGSNNPSGIPSDMDKIPFHPYYTIKDILGLLL 234

CYB_BALAC 196 HLIFLHETGSNNPTGIPSDMDKIPFHPYYTIKDILGALL 234
CYB_MACRU 196 HLLFLHETGSNNPSGINPDSDKIPFHPYYTIKDALGFML 234
cons 196 **:**********:** .* ************* ** :* 234

CYB_HORSE 235 LILLLLTLVLFSPDLLGDPDNYTPANPLSTPPHIKPEWY 273

CYB_BALAC 235 LILTLLALTLFAPDLLGDPDNYTPANPLSTPAHIKPEWY 273
CYB_MACRU 235 MLLILLTLALFSPDMLGDPDNFSPAKPTEHSSHIKPEWY 273

cons 235 ::* **:*.**:**:******::**:* . ..******* 273

CYB_HORSE 274 FLFAYAILRSIPNKLGGVLALILSILILALIPTLHMSKQ 312

CYB_BALAC 274 FLFAYAILRSIPNKLGGVLALLLSILILAFIPMLHTSKQ 312
CYB_MACRU 274 FLFAYAILRSIPNKLGGVLALLASILILLIIPLLHTSKQ 312

cons 274 *********************: ***** :** ** *** 312

CYB_HORSE 313 RSMMFRPLSQCVFWLLVADLLTLTWIGGQPVEHPYVIIG 351

CYB_BALAC 313 RSMMFRPFSQSLFWVLVADLLTLTWIGGQPVEHPYMIVG 351
CYB_MACRU 313 RSLMFRPISQTLFWILTANLITLTWIGGQPVEQPYIIIG 351

cons 313 **:****:** :**:*.*:*:***********:**:*:* 351

CYB_HORSE 352 QLASILYFSLILIFMPLASTIENNLLKW 379

CYB_BALAC 352 QLASILYFLLILVLMPVASLIENKLMKW 379
CYB_MACRU 352 QVASISYFL------------------- 360

cons 352 *:*** ** 379

No so semelhantes, pois utilizam algoritmos diferentes.