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2; 2016

ISSN 1916-9639 E-ISSN 1916-9647

Published by Canadian Center of Science and Education

Blood and Their Correlation with Blood Parameters

Mohamed A. Elblbesy1,2 & Abdelrahman T. Hereba1

1

Department of Medical Biophysics, Medical Research Institute, Alexandria University, Egypt

2

Department of Medical Laboratory Technology, Faculty of Applied Medical Science, University of Tabuk, Saudi

Arabia

Correspondence: Mohamed A. Elblbesy, Department of Medical Biophysics, Medical Research Institute,

Alexandria University, Egypt. Email:mimizizo@yahoo.com, melblbesy@ut.edu.sa

Received: January 4, 2016 Accepted: January 15, 2016 Online Published: February 15, 2016

doi:10.5539/apr.v8n2p1 URL: http://dx.doi.org/10.5539/apr.v8n2p1

Abstract

This study introduces a quantitative analysis of the coefficients of the power law model, which is used to

describe the non-Newtonian behavior of blood. Twenty blood samples from healthy donors were used to

measure the whole blood viscosity under different values of the shear rates, which are between 2.25 and 450.0 s-1.

The shear rate viscosity curves were used to calculate n (flow index) and m (the consistency of the fluid)

according to the power law model. Strong correlations (R2 > 0.5) between m and the hematocrit (HCT %),

hemoglobin (Hb), erythrocytes count (RBC), mean corpuscle volume (MCV), and mean corpuscle hemoglobin

concentration (MCHC) were obtained. Strong correlations (R2 > 0.5) between n and the RBC, MCV, and MCHC

were achieved. The relation obtained between the power law coefficients and the blood parameters in the present

investigation provides new parameters that can be used to evaluate the flow state of blood besides blood

viscosity. In addition, these parameters may be used to examine blood under pathological conditions,

representing a new tool for the diagnosis of blood abnormal conditions.

Keywords: Blood, Viscosity, Power law, HCT, Hb, RBC, MCV, MCHC

1. Introduction

Blood is a heterogeneous multi-phase mixture of solid corpuscles (red blood cells, white blood cells, and

platelets) suspended in liquid plasma, which is an aqueous solution of proteins, organic molecules, and minerals.

The rheological characteristics of blood are determined by the properties of these components and their

interaction with each other as well as with the surrounding structures. Blood rheology is also affected by external

physical conditions, such as temperature; however, in living organisms, in general, and in large mammals, in

particular, these conditions are regulated, and hence, they are subject to minor variations that cannot significantly

affect the general properties (Bodnar, 2011). Other physical properties, such as the mass density, may also play a

role in determining the overall blood rheological conduct. The rheological properties of blood and blood vessels

are affected by the intake of fluids, nutrients, and medications, although in most cases, these effects are not

substantial, except possibly over short periods of time, and normally do not have lasting consequence

(Breithaupt-Grogler et al., 1997; Vlastos et al., 2003; Marcinkowska Gapinska et al., 2007; Tripette et al., 2010).

Plasma behaves as a Newtonian fluid, whereas whole blood has non-Newtonian properties. In large vessels,

where shear the rates are high, it is reasonable to assume that blood has a constant viscosity and a Newtonian

behavior. However, in smaller vessels, or in some disease conditions, the presence of cell induces a low shear

rate and whole blood exhibits remarkable non-Newtonian characteristics, such as shear-thinning viscosity,

thixotropy, viscoelasticity and possibly a yield stress. This is largely due to the behavior of RBCs, namely, their

ability to aggregate into microstructures (rouleau) at low shear rates, their deformability into an infinite variety

of shapes without changing volume and their tendency to align with the flow field at high shear rates (Chien et

al., 1970; Schmid-Schenbein & Wells, 1969). An understanding of the coupling between the blood composition

and the physical properties of blood is essential to develop suitable constitutive models to describe blood

behavior (see the recent reviews) (Robertson et al., 2009; Robertson et al., 2008).

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www.ccsenet.org/apr Applied Physics Research Vol. 8, No. 2; 2016

Many models have been developed to describe blood viscosity. One of the most widely used forms of the general

non-Newtonian constitutive relation is a power-law model, which can be described as (Bird et al., 1987):

= (1)

Where is shear stress, is shear rate and m, n are power-law model constants. The constant m is a measure

of the consistency of the fluid: the higher the value of m, the more viscous the fluid is. n is a measure of the

degree of non-Newtonian behavior: the greater the departure from the unity, the more pronounced the

non-Newtonian properties of the fluid are. Viscosity for the power-law fluid can be expressed as (Bird R B. et al.,

1987):

= (2)

where is the non-Newtonian apparent viscosity. If n < 1, then a shear thinning fluid is obtained; such a fluid is

characterized by a progressively decreasing apparent viscosity with an increasing shear rate (Bird et al., 1987).

The present work aims to compute n and m for whole blood and test their relationship with the physiological

properties of blood. In addition, this work explores whether these power-law model constants reflect the state of

the internal structure and the interaction between blood contents.

2. Materials and Method

2.1 Sample Collection

Twenty blood samples were collected from donors of the same age and gender. All blood samples were collected

after overnight fasting in a quiet environment at normal ambient temperature. Blood was withdrawn after a 10

minute resting period and in a seated position. Blood was collected from the antecubital vein 90 s after application

of a tourniquet and without removing it to avoid the effect of pressure on the blood samples (Baskurt et al., 2009).

EDTA (1.5 mg/ml) was used as an anticoagulant. Two milliliters from each sample was sent to the clinical

laboratory to measure the hematocrit (HCT %), hemoglobin (Hb), erythrocytes count (RBC), mean corpuscle

volume (MCV), and mean corpuscle hemoglobin concentration (MCHC) using a hematology auto analyzer.

2.2 Blood Viscosity Measurement

Viscosity measurements were performed at 37 oC using a rotational viscometer fitted with a small sample cup

and a temperature controller (Brookfield Digital LVTD viscometer). The viscometer was calibrated according to

the manufacturers explicit, step-by-step instructions. Proper calibration and operation were verified through the

measurement of the viscosity of the standard solution for both Newtonian and non-Newtonian fluids.

The viscosities were measured at the different shear rates specified on a Brookfield viscometer: 2.25, 4.50, 11.25,

22.5, 45.0, 90.0, 225.0 and 450.0 sec-1. A volume of 0.5 ml of a whole blood sample was added to the viscometer.

For each test, the measurement was repeated ten times, and then, the average of the readings was taken. Each test

was performed on a new sample. The cone, cup, and spindle assembly were rinsed and carefully dried between

each trial.

2.3 Power Law Models Coefficient Calculations

The relationship between log and log was a straight line according to the power law model. log was the

y-axis and log was the x-axis. n and m were calculated graphically. n was calculated from the slope of the line

( = 1). m was calculated from the intercept of the line with the y-axis (log =

). Microsoft Excel 2010 was used to calculate both n and m. Linear regression was used after the

relationship between log and log was plotted. The equation of the linear trend line was used to compute n

and m, as mentioned above.

2.4 Statistical Analysis

The results are presented as the mean SD unless otherwise noted. Correlations were made using Pearsons

coefficient. Pearson's R2 can range from -1 to 1. A R2of -1 indicates a perfect negative linear relationship between

variables, a R2 of 0 indicates no linear relationship between variables, and a R2 of 1 indicates a perfect positive

linear relationship between variables. The data within each group were analyzed using Microsoft Excel 2010.

3. Results

The viscosity data revealed the non-Newtonian behavior of normal human blood over the given range of shear

rates (Figure 1). Shear thinning of whole blood was viewed through the relationship between and (Figure

1). A sharp decrease in was observed between 2.25 and 450.0 sec-1. The measured values of were 12.5

mPa.s at a low shear rate (2.25 sec-1), 4.89 mPa.s at 45 sec-1, and 2.38 mPa.s at a high shear rate (450.0 sec-1).

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The logarithmic relationship between and was represented as a straight line that intercepted the y-axis

(Figure 2). This relation was used to compute the values of m and n. n was in the range of 0.71630.00145 to

0.66720.00178, and m was in the range of 16.240.0064 mPa.sn to 16.1010.003 mPa.sn

n was found to be strongly correlated with RBC and MCV (R2>0.5). A positive correlation was indicated for the

relationship between n and RBC. By contrast, negative correlations were indicated for the relation between n and

HTC and MCV (Figure 3). A moderate positive correlation was found between n and MCHC (R2=0.5). A positive

weak correlation was found between n and Hb (R2<0.5). By contrast, a negative weak correlation was found

between n and HTC (Figure 3).

m was strongly correlated to RBC, Hb, HCT, MCV, and MCHC (R2>0.5). The relationships between n and the

RBC, Hb, and MCHC were inversely proportional, whereas n and HCT and MCV were directly proportional.

Figure 1. Whole blood viscosity as a function of the shear rate shows non-Newtonian behavior. Whole blood

shear thinning over the range of shear rates from 2.25 to 450 sec-1

Figure 2. The logarithmic relationship between viscosity and the shear rate shows a high degree of linearity. The

intercept with the y-axis is used to calculate m, and the slope of the line is used to calculate n

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(A) (B)

0.73 0.73

0.72 0.72

0.71 0.71

0.7 0.7

n

n

0.69 0.69

0.68 0.68

0.67 0.67

0.66 0.66

4 4.2 4.4 4.6 4.8 12 13 14 15 16 17

RBC Hb (g/dL)

(C) (D)

0.73

0.73

0.72 0.72

0.71 0.71

0.7 0.7

n

n

0.69 0.69

0.68 0.68

0.67 0.67

0.66 0.66

39 40 41 42 43 44 80 85 90 95 100 105

(E)

0.73

0.72

0.71

0.7

n

0.69

0.68

0.67

0.66

30 32 34 36 38 40 42

MCHC (g/dL)

Figure 3. n correlates to some blood parameters. (A) The equation for the relationship between n and the RBC

count is n = 0.0834 RBC + 0.3218 and R = 0.61. (B) The equation for the relationship between n and

Hb is n = 0.0104 Hb + 0.5468 and R = 0.42. (C) The equation for the relationship between n and HTC

is n = 0.0114 HTC + 1.1617 and R = 0.32. (D) The equation for the relationship between n and MCV

is n = 0.0026 MCV + 0.9362 and R = 0.6. (E) The equation for the relationship between n and MCHC is

n = 0.0039 MCHC + 0.5579 and R = 0.5

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(A) (B)

16.26 16.26

16.24 16.24

16.22 16.22

16.2 16.2

m (mPa.sn)

m (mPa.sn)

16.18 16.18

16.16 16.16

16.14 16.14

16.12 16.12

16.1 16.1

16.08 16.08

4 4.2 4.4 4.6 4.8 12 13 14 15 16 17

RBC * 106 Hb (g/dL)

(C) (D)

16.26 16.26

16.24 16.24

16.22 16.22

16.2 16.2

m (mPa.sn)

m (mPa.sn)

16.18 16.18

16.16 16.16

16.14 16.14

16.12 16.12

16.1 16.1

16.08 16.08

39 40 41 42 43 44 80 85 90 95 100 105

HCT % MCV (fL)

16.26

16.24 (E)

16.22

16.2

m (mPa.sn)

16.18

16.16

16.14

16.12

16.1

16.08

30 35 40 45

MCHC (g/dL)

Figure 4. Correlation of m to some blood parameters. (A) The equation for the relationship between m and the

RBC count is m = 0.2006 RBC + 17.058 and R = 0.63. (B) The equation for the relationship

between m and Hb is m = 0.0302 Hb + 16.592 and R = 0.64. (C) The equation for the relationship

between m and HTC is m = 0.0359 HTC + 14.689 and R = 0.6. (D) The equation for the relationship

between m and MCV is m = 0.0069 MCV + 15.522 and R = 0.71. (E) The equation for the relationship

between m and MCHC is m = 0.0116 MCHC + 16.565 and R = 0.75

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4. Discussion

Blood has previously been indicated to be a non-Newtonian fluid and exhibits a complex rheological behavior,

such as shear-thinning viscosity and thixotropy, primarily due to the presence of and interaction between cellular

elements, mainly red blood cells (RBCs), which are the most abundant component and whose mechanical

properties are inherent to the microstructure characteristic of blood (Long et al., 2005; Gijsen et al., 1999;

Thurston, 1972; Sousa et al., 2013). The non-Newtonian behavior of blood is mainly explained by three

phenomena: the tendency of erythrocytes to form three-dimensional microstructures (rouleau) at low shear rates,

their deformability (or breakup), and their tendency to align with the flow field at high shear rates. Chien et al.

indicated that when blood is at rest or at low shear rates (below 1 s-1), it seems to have a high apparent viscosity,

whereas at high shear rates, there is a reduction in the blood's viscosity (Chien et al., 1967). The blood viscosity

decreased with an increasing shear rate. For sufficiently high shear rates, the viscosity reached a plateau. Data

(minimum of 132 data points) were fitted to a curve that decayed as a monotonic exponential in response to an

increasing shear rate (David et al., 2000). The mean blood viscosity obtained by Robert S et al. at shear rates of

100, 50, and 1 s-1 were 3.26 0.43,4.37 0.60, and 5.46 0.84 mPa.s, respectively (Robert et al., 1996). The

blood viscosity was measured using a Coulter-Harkners viscometer at high shear rates (over 300 s -1). The

normal controls correspond to blood viscosity values of 3.53 mPa.s and a corrected blood viscosity of 3.46 mPa.s

(Lowe et al., 1993). Other studies reported that at low shear rates or shear stresses, the apparent viscosity was

high, whereas the apparent viscosity decreased with increasing shear and approached a minimum value under

high shear forces. At high shear rates above 100 to 200 sec-1, the viscosity of normal blood measured at 37C is

approximately 4 to 5 mPa.s and is relatively insensitive to further increases of shear. However, the viscosity

becomes increasingly sensitive to shear rates below 100 s-1 and increases exponentially as the shear rate is

decreased. Nominal values for the viscosity of normal blood are approximately 10 mPa.s at 10 s-1, 20 mPa.s at 1

s-1, and 100 mPa.s at 0.1 s-1. Thus, at lower shear rates, the blood viscosity becomes extremely sensitive to the

decrement in shear forces. At stasis, normal blood has a yield stress in the range of 2 to 4 mPa (Merrill EW, 1969;

Rampling, 1988; Chien., 1975; Rand et al., 1964). Our results (Figure 1.), obtained using a rotational viscometer,

are in good accord with the results obtained in the literature for both the non-Newtonian behavior of whole blood

and for the values of the apparent blood viscosity in the given range of shear rates. The difference in the apparent

viscosity at a specific shear rate, especially under low shear rates, from previous studies is due to the difference

in the hematocrit percentages used in our study.

Experiments with the shear-rheometer in a Couette geometry (Behbahani et al., 2009) were used to

accommodate the Parabolic model to obtain experimental data for the shear rate and shear stress. Six rheological

measurements of human blood with 47 % hematocrit were analyzed, and the results for the Parabolic model are

compared with the widely used Power law model. For the latter parameters, K (consistency index) and n (Flow

index) were determined using the least-squares method (Marn & Ternik, 2003). The values of K were in the

range of 1.568E-2 Pa.s to 1.8567E-2 Pa.s, and the values of n were between 7.4815E-1 and 7.18E-1. Bernasconi

et al. and Kar et al. have shown that the power law regression method is suitable for blood viscosity

quantification. Bernasconi et al. conducted their series of experiments on exclusively normal blood samples; Kar

et al. and later Hussain et al., from the same research group, conclusively proved that not only normal blood but

also other pathological blood types follow a power law model (Mohammad et al., 1990). The results obtained are

extremely useful for understanding the rheological behavior of blood. A few studies determined the correlation

of the power-law coefficients with the hematological and biochemical parameters in the form of the constitutive

equation. Easthope and Brooks used a constitutive function that was first employed by Walburn and Schneck to

describe the flow properties of whole blood that relate the shear stress measured in a viscometer to the shear rate

and hematocrit of the sample (Walburn & Schneck, 1976; Easthope & Brooks, 1976). Hussain et al. reported that

because n of the power law model is the non-Newtonian behavior index and k is the flow consistency index of

blood, they are naturally dependent on the constituents of blood, such as hematocrit, fibrinogen, cholesterol, and

so on. It is possible to have such a relationship between the power law coefficients and the above-mentioned

parameters in the form of a mathematical equation by using non-linear regression analysis (Mohammad et al.,

1990; Hussain et al., 1994; Hussain et al., 1995). Panagiotis used m = 14.67 mPa.s and n = 0.7755 for his

simulation of blood flow; this simulation represented a typical behavior of blood flow according to the power

law model (Panagiotis Neofytou, 2004). For healthy controls, Hussain et al. reported that the experimental value

of n was 0.708 and the calculated value of n was 0.713, with 0.25 as the standard deviation of the difference

between the experimental and calculated values. Alternatively, the value of normalized K for healthy controls

(divided by 17.0 mPa.sn) was 0.980 and 0.995 for the experimental and calculated values, respectively, with

0.106 as the standard deviation of the difference between the experimental and calculated values (Mohammad et

al., 1990). The calculated n and K from the research of Hussain et al. were calculated as functions of hematocrit,

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fibrinogen, and cholesterol; their results proved that n and K are correlated strongly with the selected blood

parameters (Mohammad et al., 1990). Blood viscosity can be affected by various factors (e.g., hemospherine,

glucose, and proteins), the most important of which is hematocrit, which denotes the percentage of blood volume

that is occupied by red blood cells (Beatriz et al., 2006). Whole blood viscosity correlated with HCT (r=0.63, p <

0.001 at low shear and r =0.84, p < 0.001 at high shear) (Craig et al., 2006). Karsheva et al. proved the

dependence of the power law coefficients on both RBC and HCT (M. Karsheva et al., 2009). They obtained the

value of n<1 and m between 0.9 to 1.3 mPa.sn. A key feature of our study is to examine the correlations between

m and n with the blood parameters that are mainly related to erythrocytes (such as HCT %, Hb, RBC, MCV, and

MCHC) because erythrocytes play a major role in the non-Newtonian behavior of blood. The values of n and m

obtained from our study are in a good agreement with previous studies. The correlations between n and both

RBC and MCV were strong (Figure 3), in accordance with the dramatic dependence of the number and size of

erythrocytes on the flow behavior of the whole blood. The correlations between n and other blood indices were

moderate to weak (Figure 3) because the other blood parameters have stronger effects on the physiological and

biochemical properties of whole blood than the macro-rheological behavior of whole blood.

RBC and their related indices are well known to affect viscosity (Chao-Hung, 2004). Chao-Hung found strong

positive correlations between whole blood viscosity and the RBC count, Hb, and HCT. David M. et al. found

that the blood viscosity increased with increasing hematocrit (David et al., 2000). M, which depends on the

physiological parameters of blood, was strongly correlated to the blood indices considered in this study (Figure

4). HCT and Hg increase blood viscosity under different values of shears rates. This linear logarithmic

relationship between viscosity and the shear rates corresponds to lines with higher slopes and increasing

intercepts of the line, with the y-axis representing the shear rates. This leads to an increase of the value of blood

indices with increasing m. From the dependence of m on the internal structure of the liquid under investigation,

our results showed strong positive correlations with all of the blood indices.

5. Conclusion

From this study, we conclude that blood behaves as a non-Newtonian fluid, exhibiting a shear thinning behavior.

Many models could be used to describe the non-Newtonian behavior of blood, the easiest of which is the power

law model. Much information could be extracted from the power law model using an easy computational method.

This information is related to the physiological parameters of blood; as a result, the extraction of information

from the power law model is a powerful tool to evaluate blood under normal and abnormal conditions. The

power law coefficients, n and m, used in this study and in previous studies showed promise as a method to

describe the state of blood under investigation based on the correlations between the coefficients and blood

parameters (such as HCT, Hb, RBC, MCV in this study and fibrinogen and cholesterol in other study). Further

research should be performed to study the correlation between the power law coefficients and the blood

physiological and chemical parameters under both normal and pathogenic conditions. In addition, research

should be performed to evaluate the values of the power law model coefficients for blood at low shear rates.

References

Baskurt, O., Boynard, M., Cokelet, G., Connes, P., Cooke, B. M., Forconi, S., ... & Nash, G. (2009). New

guidelines for hemorheological laboratory techniques. Clinical hemorheology and microcirculation, 42(2),

75-97.

Behbahani, M., Behr, M., Hormes, M., Steinseifer, U., Arora, D., Coronado, O., & Pasquali, M. (2009).

Computational Fluid Dynamics of Blood Flow. European Journal of applied mathematics, 20, 363-397.

http://dx.doi.org/10.1017/S0956792509007839

Bird, R. B., Curtiss, C. F., Armstrong, R. C., & Hassager, O. (1987). Dynamics of polymeric liquids. In Kinetic

theory (Volume 2, p. 437). New York: Wiley-lnterscience.

Bodnr, T., Sequeira, A., & Prosi, M. (2011). On the shear-thinning and viscoelastic effects of blood flow under

various flow rates. Applied Mathematics and Computation, 217(11), 5055-5067. http://dx.doi.org/10.

1016/j.amc.2010.07.054

Breithaupt-Grgler, K., Ling, M., Boudoulas, H., & Belz, G. G. (1997). Protective effect of chronic garlic intake

on elastic properties of aorta in the elderly. Circulation, 96(8), 2649-2655. http://dx.doi.org/10.

1161/01.CIR.96.8.2649

Broberg, C. S., Bax, B. E., Okonko, D. O., Rampling, M. W., Bayne, S., Harries, C., ... & Gibbs, J. S. R. (2006).

Blood viscosity and its relationship to iron deficiency, symptoms, and exercise capacity in adults with

cyanotic congenital heart disease. Journal of the American College of Cardiology, 48(2), 356-365.

7

www.ccsenet.org/apr Applied Physics Research Vol. 8, No. 2; 2016

Chien, S. (1975). Biophysical behavior of red cells in suspensions. The red blood cell, 2(4), 1031-1133.

http://dx.doi.org/10.1016/B978-0-12-677202-9.50019-8

Chien, S., Usami, S., Dellenback, R. J., & Gregersen, M. I. (1970). Shear-dependent deformation of erythrocytes

in rheology of human blood. American Journal of Physiology--Legacy Content, 219(1), 136-142.

Chien, S., Usami, S., Dellenback, R. J., Gregersen, M. I., Nanninga, L. B., & Guest, M. M. (1967). Blood

viscosity: influence of erythrocyte aggregation. Science, 157(3790), 829-831. http://dx.doi.org/10.1126/

science.157.3790.829

Easthope, P. L., & Brooks, D. E. (1979). A comparison of rheological constitutive functions for whole human

blood. Biorheology, 17(3), 235-247.

Eckmann, D. M., Bowers, S., Stecker, M., & Cheung, A. T. (2000). Hematocrit, volume expander, temperature,

and shear rate effects on blood viscosity. Anesthesia & Analgesia, 91(3), 539-545. http://dx.doi.org/10.

1097/00000539-200009000-00007

Gijsen, F. J. H., Allanic, E., Van de Vosse, F. N., & Janssen, J. D. (1999). The influence of the non-Newtonian

properties of blood on the flow in large arteries: unsteady flow in a 90 curved tube. Journal of biomechanics,

32(6), 601-608. http://dx.doi.org/10.1016/S0021-9290(99)00015-9

Ho, C. H. (2004). White blood cell and platelet counts could affect whole blood viscosity. Journal-Chinese

Medical Association, 67(8), 394-397.

Hussain, M. A., Kar, S., & Puniyani, R. R. (1999). Relationship between power law coefficients and major blood

constituents affecting the whole blood viscosity. Journal of Biosciences, 24(3), 329-337.

Hussain, M. A., Puniyani, R. R., & Kar, S. (1994). Quantification of blood viscosity using power law model in

cerebrovascular accidents and high risk controls. Clin. Hemorheo L., 14, 685-696.

Hussain, M. A., Puniyani, R. R., & Kar, S. (1995). Blood viscosity autoregulation factor in cerebrovascular

accidents and high risk cases-> a new approach. Clinical hemorheology, 1(15), 61-71.

Karsheva, M., Dinkova, P., Pentchev, I., & Ivanova, T. (2009). Blood rheology-a key for blood circulation in

human body. Journal of the University of Chemical Technology and Metallurgy, 44(1), 50-54.

Long, J. A., ndar, A., Manning, K. B., & Deutsch, S. (2005). Viscoelasticity of pediatric blood and its

implications for the testing of a pulsatile pediatric blood pump. ASAIO journal, 51(5), 563-566.

http://dx.doi.org/10.1097/01.mat.0000180353.12963.f2

Lowe, G. D., Fowkes, F. G., Dawes, J., Donnan, P. T., Lennie, S. E., & Housley, E. (1993). Blood viscosity,

fibrinogen, and activation of coagulation and leukocytes in peripheral arterial disease and the normal

population in the Edinburgh Artery Study. Circulation, 87(6), 1915-1920. http://dx.doi.org/10.1161/

01.CIR.87.6.1915

Marcinkowska-Gapiska, A., Gapinski, J., Elikowski, W., Jaroszyk, F., & Kubisz, L. (2007). Comparison of

three rheological models of shear flow behavior studied on blood samples from post-infarction patients.

Medical & biological engineering & computing, 45(9), 837-844. http://dx.doi.org/10.1007/s11517

-007-0236-4

Marn, J., & Ternik, P. (2003). Use of Quadratic model for modelling of fly ash-water mixture. Applied Rheology,

13(6), 286-296.

Merrill, E. W. (1969). Rheology of blood. Physiol. Rev, 49(4), 863-888.

Neofytou, P. (2003). Comparison of blood rheological models for physiological flow simulation. Biorheology,

41(6), 693-714.

Rampling, M. W. (1988). Red cell aggregation and yield stress. Clinical blood rheology, 1, 11-44.

Rand, P. W., Lacombe, E., Hunt, H. E., & Austin, W. H. (1964). Viscosity of normal human blood under

normothermic and hypothermic conditions. Journal of Applied Physiology, 19(1), 117-122.

Robertson, A. M., Sequeira, A., & Kameneva, M. V. (2008). Hemorheology. In Hemodynamical Flows

Modeling, Analysis and Simulation, Birkhuser Basel (pp. 63-120).

Robertson, A. M., Sequeira, A., & Owens, R. G. (2009). Rheological models for blood. In Cardiovascular

mathematics (pp. 211-241). Springer Milan. http://dx.doi.org/10.1007/978-88-470-1152-6_6

8

www.ccsenet.org/apr Applied Physics Research Vol. 8, No. 2; 2016

Rosenson, R. S., McCormick, A., & Uretz, E. F. (1996). Distribution of blood viscosity values and biochemical

correlates in healthy adults. Clinical Chemistry, 42(8), 1189-1195. http://dx.doi.org/10.1016/0021

-9150(94)94059-2

Salazar-Vazquez, B. Y., Intaglietta, M., Rodrguez-Morn, M., & Guerrero-Romero, F. (2006). Blood pressure

and hematocrit in diabetes and the role of endothelial responses in the variability of blood viscosity.

Diabetes Care, 29(7), 1523-1528. http://dx.doi.org/10.2337/dc06-0323

Schmid-Schnbein, H., & Wells, R. (1969). Fluid drop-like transition of erythrocytes under shear. Science,

165(3890), 288-291.

Sousa, P. C., Carneiro, J., Vaz, R., Cerejo, A., Pinho, F. T., Alves, M. A., & Oliveira, M. (2014). Shear viscosity

and nonlinear behaviour of whole blood under large amplitude oscillatory shear. Biorheology, 50(5-6),

269-282.

Thurston, G. B. (1972). Viscoelasticity of human blood. Biophysical journal, 12(9), 1205-1217.

http://dx.doi.org/10.1016/S0006-3495(72)86156-3

Tripette, J., Loko, G., Samb, A., Gogh, B. D., Sewade, E., Seck, D., ... & Brudey, K. (2010). Effects of hydration

and dehydration on blood rheology in sickle cell trait carriers during exercise. American Journal of

Physiology-Heart and Circulatory Physiology, 299(3), H908-H914. http://dx.doi.org/10.1152/ajpheart.

00298.2010

Vlastosa, G. A., Tangneyb, C. C., & Rosensonc, R. S. (2003). Effects of hydration on blood rheology. Clinical

hemorheology and microcirculation, 28, 41-49.

Walburn, F. J., & Schneck, D. J. (1976). A constitutive equation for whole human blood. Biorheology, 13(3),

201-210.

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