Agilent1100PLCusingChemstationSoftware

1.TurnonallthemodulesoftheAgilent1100.Alwaysturnmodulesonbeforeopeningsoftware.

2.TurnonComputer.Nopasswordisneeded

3.GounderStart,AllPrograms,AgilentChemstation,Instrument1online.ThefollowingMethod
andRunControlwindowshouldappear.

4.TurnonPumpbyclickingonGreysquareboxunderinstrumenticon.

Clickonsquarebox

5.AnewboxwillappearaskingTurnonallpumps.ClickYes.

6.TurnonColumnThermostatbyclickingoninstrumentcontrolwhichisgreysquarearrow.

Clickondownwards

arrow.

7.AnewwindowwillappearaskingtoTurnontheThermostat.ClickYes.

8.Ifyoualreadyhaveamethod,UnderMethod,chooseLoadMethodtochooseyourmethod.

9.ChooseyourmethodunderChem32/1/Methods.

9.Ifyouneedtocreateamethod,UnderMethod,chooseEditEntireMethodtocreateanew
method.

7.EditMethodwindowappearswhereyoucancheckoffpartsofmethodtobechanged.HitOK.

8.FillinMethodInformationandhitOK.

9.SetupPumpParametersWindow.FillinFlowrate,Solvents,CreateGradientorrunisocratic.
Youhaveuptofourdifferentsolventstouse.

a.UseInsertorAppendlinetocreategradient.HitOK.

b.UnderDisplay,youcanchangefromTimetabletoSolventstoseeyourgradient.

10.SetupInjectionParameters.Thereisa100lsampleloop.Youcaninjectfrom0.1to100l.
Thereisaneedlewashvialinholder91.Besurethereisorganicinthevial.HitOK.

11.DADSignals:Youcanchooseupto5differentwavelengths.HitOK.BWisforbandwidth.

12.ColumnThermostatparameters.Canprogramfromambientto80CorchooseNotControlled.
HitOK.

13.SignalDetailsWindow:AddtoMethodallyourdifferentwavelengths.HitOK.

14.EditIntegrationEvents.HitOK.

16.SetSpecificationforReport.HitOK.

17.TheInstrumentDataCurvescanbeoverlaidwithyourspectrum,likepercentcomposition.Check
offwhatyouwanttoseeinyourspectrum.HitOK.

18.RunTimeChecklist.HitOK.

19.UnderMethod,ChooseSaveMethodAs.BesuretosaveunderMethods[c:/chem32/1/methods]

20.UnderView,chooseOnlineSignals1.ClickonChangetoaddorremoveOnlinesignals.

21.UnderSequence,ChooseSequenceTable.

a. UnderVial,enterthelocationofthevial.(Youdontneedtotypevial)
b. UnderSample,givenameandcanadddescriptionofsampleinSampleinfojustabove.
c. UnderMethodName,choosenameofmethod.
d. UnderInjection/locationplacea1for1injection.Ifyouwantmultipleinjectionsofsame
sample,youcanadd2,3,4,etc.
e. UnderSampletype,youcanchooseSample,calibrationorcontrol.
f. UseInsertorAppendLinetoaddmoreinjections.

22.UnderSequence,chooseSaveSequenceTemplateas.Saveasyournameandthedate

23.Opensequencetableagain.HitRunSequencewhenready.

RunSequence

24.YoucanalwayspresstoSTOPbuttontostoptherunatanytime.

25.Areportwillautomaticallybegenerated.HitPrintandsavereportaspdf.

26.ShutdownChemstation,thenallthemodules.