Department of Histology and Embryology

Faculty of Medicine, PU, Olomouc, CZ

Histology practical No. 4

Topics: 1- STRUCTURAL AND FUNCTIONAL CHARACTERISTICS
OF GLANDULAR EPITHELIA AND THEIR CLASSIFICATION
(pre-lab rev.ppt).
2- RECOGNITION OF SEROUS AND MUCOUS GLADS IN STAINED
HISTOLOGY AND PC-MONITORED SLIDES OR PRINTED IMAGES.
3- REVISION OF THE ULTRASTRUCTURE OF SPECIALIZED PROTEIN-
SYNTHESIZIG AND MUCUS-SECRETING EPITHELIAL CELLS.
(Recommended textbook and Atlas with CD-ROM and websites)
4- COMPARE THE STRUCTURE OF EXOCRINE AND ENDOCRINE GLANDS.

Contents:

A. EXOCRINE GLANDS
1. Parotid gland (glandula parotis, sl. no. 19) sect., H&E stain.
This is a large
purely serous-sec
reting gland that
contains many
serous cells arran-
ged in multiple
compound acini
(alveoli). Study the serous cells at high magnification and
note the shape and location of their nuclei. Draw a simple
alveolus containing a few serous cells. They are
practically everywhere in this section. What kind of
ultrastructures are located under the nucleus of these cells? Among the alveoli, there are many
excretory ducts lined with different epithelial cells. Connective tissue is stained pale pink, and
adipose cells are large and empty-looking.

2. Duodenum (duodenum, sl. no. 26) sect., H&E stain.

This slide shows part
of a transverse
section of the initial
portion of small
intestine (duodenum)
that is composed of
several layers of
different tissues. The
surface epithelium is
lined with a simple
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 columnar epithelium (locate it first). Then go to the glandular tissue under the epithelium
(arrow) and locate the multiple, compound mucous tubuli of the purely mucus-secreting
gland there (Brunners gland). Draw a simple tubulus containing a few mucous cells. Note the
shape and position of the nuclei. The next layers in this slide contain smooth muscle mostly.

3. Submandibular gland (glandula submand., sl.n. 20) section., H&E stain.

This mixed-secretion
producing (seromucous
secretion with pre-
valence of the serous
component) gland is
located in the tissue
under the mandible.
Practically everywhere
you can locate both, the serous alveoli (these prevail,
arrow) and some mucous tubules (arrowhead) containing
pale-stained cells. Locate some serous demilunes at the terminal portions of mucus tubules.
Note the arrangement of the glandular tissue into lobules and the presence of several ducts
there.

4. Sublinqual gland (glandula sublinqualis, sl.n. 21) sect., H&E stain.

This mixed
secretion-produ-
cing gland (sero
mucous secreti
on with preva
lence of the
mucous
component) is
located in the
tissue under the
tongue. Find the lobule where the mucous tubules prevail (arrow) and study the mucous cells
and serous demilunes. Compare these with the other lobules where the serous cells are in
majority.

5. Trachea (trachea, sl.n. 34) t.s., H&E stain.

In transverse section
of the trachea, find
the inner surface of
this tube and locate
the pseudostratified columnar epithelium with kinocilia.
Note, that the kinocilia are absent on the surface of pale
or empty-looking cells, called goblet cells. At high power
lens, locate one of these cells and study its shape and
location of its nucleus. This is an example of the
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 intraepithelial single-cell gland. What is the quality of its product? Internal layers of the
trachea in this slide also contain some other glands. Evaluate the quality of their product.

B. ENDOCRINE GLANDS
6. Suprarenal gland (Nadledvinka, sl.n. 15) section., H&E stain.
This is a
section of the
suprarenal
gland that is the
example of the
endocrine gland
with fascicular
arrangement of
secretory
epithelial cells.
Locate the cords of cells in the superficial layers of this section (arrow). Note the absence of
ducts. What is the way the secretory cells release and distribute their product to their body
targets? How we call these products?

7. Thyroid gland (glandula thyroidea, sl. n. 13) sect., H&E stain.

Thyroid gland
is an endocrine
gland with
follicular
arrangement of
secretory
epithelial cells.
The follicles
are lined
mostly with
simple cuboidal
endocrine cells that surround the central area filled with an acidophilic material called colloid
(arrow). Study the shapes of epithelial cells and note the absence of ducts. Within the
connective tissue septa, between lobules of the secretory parenchyma, there are several blood
vessels and some adipose tissue cells.

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8. Identify as many structures in this picture as you can.

11. In your selfstudy, revise the ultrastructure of specialized protein synthesizig

and mucus secreting epithelial cells.

(Use the recommended textbook and atlas with CD-ROM)

Websites:
http://www.kumc.edu/instruction/medicine/anatomy/histoweb/glands/glands.htm
http://www.anatomyatlases.org/MicroscopicAnatomy/Section02/Plate0226.shtml
http://www.meddean.luc.edu/lumen/MedEd/Histo/frames/h_frame1.html
http://www.path.uiowa.edu/cgi-bin-pub/vs/vl_browse.cgi?cat=h_epithelium
http://www.lab.anhb.uwa.edu.au/mb140/default.htm

4-EpithGland Practical.doc dk-3/2009