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2. Add one DPD Chlorine Powder Pillow to the other cell. Place the
cap on the cell and shake it gently for 20 seconds or until all the
powder has dissolved. Wipe the cell clean of fingerprints and
dust Wait 3 minutes for the powder to react. This will be called
the prepared sample.
3. Remove the cap from the instrument. Place the blank in the cell
holder with the diamond mark facing you. Tightly cover the cell
with the instrument cap.
4. Press the ZERO button. The instrument will turn on and the
display will show---then 0.00.
5. Remove the blank from the instrument and place the prepared
sample into the cell holder with the diamond mark facing you.
It is important to exercise special caution when handling the chemicals used in the
various methods for chlorine residual determination because several of them are highly
toxic. The phenylarsine oxide (PAO) and standard arsenite solution used in the
iodometric and amperometric methods are poisonous. Clean up spills quickly and
carefully with disposable rags or towels and be sure to wash hands thoroughly before
eating or smoking. The DPD oxide indicator used in the DPD titrimetric and colorimetric
methods is also very poisonous and the same precautions must be exercised in its use.
As with all laboratory testing, handle glassware carefully. Lab glassware can break
easily and leave very sharp pieces of glass. If breakage does occur, use a good broom
and dust pan. Do not pick up by hand or use a towel or sponge that will be used again.
Keep rubber gloves and safety glasses on hand and use them. Always use a pipetting
bulb. Never pipette anything by mouth.
SAMPLING
Due to the manner in which calculations are set up for the iodometric back -titration
methods using iodine titrant, the test procedures used for these methods can measure
the Cl2 residual only between 0.5 and 4.0 mg/L, in 0.5 mg/L increments, unless
3
2. Pour 200 mL of sample or sample dilution into the container for use with the
apparatus.
5. Add 4 mL pH 4.0 acetate buffer solution (or enough to lower the pH to between 3.5
and 4.2).
6. Titrate, while stirring, with 0.0282 N iodine solution. The needle on the meter will
remain almost stationary until the end-point is approached. As the end-point is
approached, each addition of iodine causes a temporary movement of the needle
(in a clockwise or upscale direction) with a return to its original position. The
end-point is reached when a small addition of iodine titrant gives a definite
movement of the needle, and the needle does not return quickly to its original
position.
8. Check the normality of the iodine solution at least daily by completing steps 1 -7
using 200 mL of distilled water.
The presence of nitrogen trichloride or chlorine dioxide will interfere because they titrate
partially as free chlorine. Organic chloramines have the same effect. Free halogens
other than chlorine (bromine, iodine and fluorine) interfere by titrating as free chlorine.
Copper and silver cause interference by poisoning the electrode. Very low
temperatures can slow the reaction time, but do not affect the precision of the test. The
violent stirring of some commercial titrators can cause loss of chlorine through
volatilization.
DESCRIPTION OF TEST
When PAO is added to a sample buffered to pH 4.0, the excess iodine in the sample is
discharged. When the end-point is reached, a change will occur in the electric current
4
See Appendix B at the end of this chapter for the procedure for the preparation of the
reagents used in this method.
EQUIPMENT
3. 5 mL measuring pipettes
LABORATORY PROCEDURE
2. Pour 200 mL of sample or sample dilution into the container to be used with the
apparatus.
3. Add 1 mL of 5% potassium iodide (KI) solution to the container and mix thoroughly.
4. Add 1 mL pH 4.0 acetate buffer solution to the container and mix thoroughly.
5. Titrate, while stirring, with a 0.00564 N phenylarsine oxide (PAO) solution. The
instruments meter indicator will deflect counter clockwise or downscale with each
increment of titrant added to the container. As the end-point is approached, the
indicator movement will slow. Titrant increments should also be decreased.
Successive burette readings should be made before each increment addition when
the meter indicates the end-point is approaching.
6. Subtract the last small increment that produces no response in the meter indicator
and record the volume of PAO titrant used.
5
The presence of nitrogen trichloride or chlorine dioxide will interfere because they titrate
partially as free chlorine. Organic chloramines have the same effect. Free halogens
other than chlorine (bromine, iodine and fluorine) interfere by titrating as free chlorine.
Copper and silver cause interference by poisoning the electrode. Very low
temperatures can slow the reaction time, but do not affect the precision of the test. The
violent stirring of some commercial titrators can cause loss of chlorine through
volatilization.
DESCRIPTION OF TEST
The DPD titration method also uses a color change to determine the chlorine residual.
In the presence of chlorine, the DPD indicator solution has a red color. The more
chlorine that is present, the darker the red color will be. Ferrous ammonium sulfate
(FAS) is used as the titrant in this method. When the end-point has been reached, the
red color will disappear. The amount of chlorine present is equal to the amount of FAS
titrated.
The buffer and indicator are commercially available as a combined reagent in stable
powder form.
EQUIPMENT
4. Add 100 mL of sample, mix well, and let stand for 2 minutes to allow color to fully
develop.
5. Titrate with standard ferrous ammonium sulfate titrant (FAS) until the red color first
completely disappears.
7. Check the normality of the FAS titrant at least weekly against standard potassium
dichromate.
The interferences which are likely to be encountered in water are oxidized manganese
and high levels of mono-chloramine. To correct for this, place 5 mL of phosphate buffer
solution and 0.5 mL sodium arsenite solution in the titration flask. Add 100 mL of
sample and mix. Add about 1 g KI crystals and 5 mL DPD indicator, mix and titrate with
FAS solution until red color is discharged. Use this blank reading in subsequent
calculations. Copper up to 10 mg/L is controlled by EDTA in reagents. Free halogens
may react with the DPD indicator and appear as free available chlorine. As in most
tests which depend on color changes, sample color, turbidity, and high concentrations of
organic matter may cause some interference.
DPD COLORIMETRIC
DESCRIPTION OF TEST
15 mL test tubes
1 cm sample cuvettes
10 mL measuring pipette
1 mL pipettes, graduated to 0.1 mL
250 mL Erlenmeyer flask
LABORATORY PROCEDURE FOR DPD COLORIMETRIC METHOD
5. Fill photometer or colorimeter cell from flask and read each standard at 515 nm
wavelength.
7. Pipette 0.5 mL phosphate buffer solution into a test tube of appropriate volume.
NOTE: Test tubes should have a capacity of at least 15 mL and either have a screw -on
cap or be capable of accepting a rubber stopper which does not cause sample to
overflow when put in place.
10. Add 10 mL sample, stopper or cap, mix well, and let stand for two minutes to allow
color to develop.
11. Pour into photometer tube (also called a cuvette) and take reading of photometer in
%T.
8
The mg/L Cl2 is read directly from the standard curve prepared by using potassium
permanganate (KMnO4) as the standard.
NOTE: Use appropriate sample volume and glassware to ensure that the expected
sample concentration falls within the range of the direct reading colorimeter
concentration readout.
4. Place sample cell in sample compartment, depress actuator button and read mg/L
concentration from the appropriate meter scale.
5. If sample concentration is within the mg/L range of the colorimeter, record reading.
6. If sample concentration exceeds mg/L range of the colorimeter, repeat steps 1-5, as
necessary, using a diluted sample.
The mg/L Cl2 is read directly from the meter dial of the direct reading colorimeter.
The interferences which are likely to be encountered in water are oxidized manganese
and high levels of mono-chloramine. To correct for this, place 5 mL of phosphate buffer
solution and 0.5 mL sodium arsenite solution in the titration flask. Add 100 mL of
sample and mix. Add about 1 g KI crystals and 5 mL DPD indicator and mix. Measure
the apparent chlorine as manganese and use this blank reading in subsequent
calculations. Copper up to 10 mg/L is controlled by EDTA in reagents. Free halogens
may react with the DPD indicator and appear as free available chlorine. As in most
tests which depend on color changes, sample color, turbidity and high concentrations of
organic matter may cause some interferences. Compensate for color or turbidity in the
colorimetric procedure by using an untreated sample blank.
9
DESCRIPTION OF TEST
The Orion specific ion electrode method uses an electrical measurement similar to pH
meter test procedure to determine the chlorine residual. Samples are prepared for
measurement by adding an iodide reagent and an acid reagent. Chlorine in the sample
reacts with iodide under acid conditions to form iodine. The iodine is, in turn, measured
by the electrode and indicated on a pH or specific meter as mv or concentration.
EQUIPMENT
LABORATORY PROCEDURE
b. Add 1 mL chlorine water to beaker and stir gently for 2 minutes to allow
complete reaction.
e. Add 10 mL chlorine water to the beaker. Stir gently for 2 minutes to allow
complete reaction, then STOP STIRRING.
f. The meter reading should be approximately 29 mv. If the reading is less than
26 mv, the electrode instruction manual should be reviewed.
4. Using 4 cycle semilogarithmic graph paper, construct a calibration curve with 1 ppm
at the center of the logarithmic scale and 000.0 mv at the center of the linear axis.
(The linear range should be from -60 to +60 mv.) Plot one point at 000.0 mv and 1
ppm on the graph and a second point at 29 mv and 10 ppm. Draw a straight line
through these points extending to at least 0.1 ppm. Use this graph for all chlorine
residual measurements.
5. Place the electrode in the 1 ppm standard so that the reference element is
submerged. DO NOT STIR.
6. Set meter function switch to mv setting and adjust calibration to read 000.0 mv.
10. Mix contents of beaker thoroughly, then stop stirring and let stand for 2 minutes to
allow for complete reaction.
11. Place the electrode in the prepared sample so that the reference element is
submerged.
Strong oxidizing agents, including iodate, bromine, cupric ion and oxidized manganese,
that can convert iodide to iodine, interfere with the method. Silver and mercuric ions
must be below approximately 10 mg/L. Chromate ion, an interference in the
amperometric methods, does not interfere with this procedure. Color and turbidity do
not interfere with the method.
11
DESCRIPTION OF TEST
The Hach Companys Model CN-66 Total Chlorine Residual Test Kit also uses the DPD
reagent to determine total chlorine residual values. The DPD reagent is packaged in
premeasured amounts stored in disposable powder pillows. The DPD reagent is added
to a portion of sample. If chlorine is present, a pinkish-red color will develop. The color
produced is visually compared to standard colors representing various concentrations of
total chlorine residual. The total chlorine residual is read from this comparison.
1. Fill both sample vials to the 5 mL graduation with fresh sample to be tested.
2. Add the contents of one total chlorine residual DPD powder pillow to one of the
vials.
5. Place the appropriate DPD chlorine color wheel in the color comparator.
6. Place the treated sample vial in the right hand (nearest to center) hole of the
comparator.
7. Place the untreated sample vial in the left hand (nearest to edge) hole of the
comparator.
8. Point the comparator towards a diffused outside light or a steady indoor fluorescent
light source.
9. Rotate the color wheel until there is a match of color for the two vials.
12
10. Read the total chlorine residual mg/L of the sample from the comparator and
record.
The mg/L Cl2 is read directly from the color wheel comparator indicator.
CN-66 METHOD
The same interferences apply to this test which are noted for other DPD procedures,
however, there are no provisions for compensating for most of these interferences. As
in any procedure using visual color comparison, the individual perceptions of the
analysts as well as the condition of the glassware, the color wheel, and the light source,
can all affect the results of the test. Limit the amount of error from these factors by
thoroughly cleaning the vials after each use, maintaining the comparator and color
wheel, and using a stable and constant light source.
D. Standardization
2. Titrate with PAO solution, using starch solution as an indicator, until blue
disappears.
1. Iodate Method
2. Dichromate Method
D. Standardization
B. Add the correct amount of the exactly standardized 0.1 N iodine solution to
yield a 0.0282 N solution.
D. Store iodine solutions in amber bottles or in the dark, and protect from
exposure to direct sunlight. Do not use rubber stoppers; keep iodine from all
contact with rubber.
A. Dissolve 201.2 mg primary standard grade potassium iodate (KIO3), dried for 1
hour at 103C, or 183.3 mg primary standard grade anhydrous potassium
bi-iodate (KH(IO3)2V) in distilled water.
C. Store in glass bottles in the dark and protect from exposure to direct sunlight.
A. Dissolve 50 g KI in freshly boiled and cooled distilled water and dilute to 1 liter.
15
NOTE: Use forceps or tongsdo not handle weighing bottle with fingers.
B. In weighing bottle, weigh out approximately 4.95 g arsenic trioxide (As 2O3).
H. Dilute to 250 mL and saturate the solution with carbon dioxide (CO 2) by
bubbling CO2 gas through the solution for a few minutes.
NOTE: This converts the sodium hydroxide (NaOH) to sodium bicarbonate (NaHCO 3).
C. Pour into 1 liter boiling distilled water, stir and let settle overnight.
NOTE: This chemical is also known as (ethylenediamine) tetraacetic acid sodium salt.
D. Dilute to 1 liter and store in a brown glass-stoppered bottle and discard when
discolored.
C. Dilute to 1 liter.
If a direct concentration readout colorimeter is used, the DPD and buffer reagents
should be prepared or ordered in accordance with the instrument manufacturers
instructions. If the Hach DR100 colorimeter is used, the prepared DPD powder
pillows used with the Hach direct reading colorimeters may be purchased from the
Hach Company at the following address:
Hach Company
P.O. Box 389
Loveland, Colorado 80539
II. Prepare the chlorine water (approximately 100 ppm) by diluting 1 mL hypochlorite
solution (household chlorine bleach) to 500 mL with distilled water.
The amount of residual chlorine left in the chlorinated water after the required contact
period, can be experimentally determined by using any of the following tests:
Orthotolidine test
In this test, 10 ml of chlorinated sample of water is taken after the required contact
period, in a glass tube. To this 0.1 ml of orthotolidine solution is added. The color formed
is observed after 5 minutes. The formation of yellow color normally indicates the
presence of chlorine (either combined or free) in the water. The more yellow the color,
the greater, is the chlorine residual. The amount of residual chlorine can be ascertained
by comparing the colour developed in the glass tube with the standard colors already
kept in the laboratory. This test, is therefore, very simple and does not require much
technique or time. Under normal conditions, a lemon yellow color is satisfactory for
public water supply. The orthotolidine test will normally give the total residual chlorine
present in water. However, it may be adjusted so as to give separately the quantities of
19
free residual as well as combined residual of chlorine. The free residual chlorine forms
the yellow color during the first 5 seconds of the addition of orthotolidine, while the
combined residual chlorine goes on forming the colour for about 5 minutes. Hence, the
colour after 5 seconds will give the quantity of free residual chlorine, and the colour after
5 minutes will give the free and combined chlorine. The difference in value between the
two values is the combined chlorine. The orthotolidine test, however, is not accurate,
because the impurities such as iron, manganese, nitrate etc., are likely to cause a false
yellow colour, and indicating wrong and increased chlorine residual.
To overcome this problem Orthotolidine Arsenite (OT A) test is carried out. Sodium
arsenite is added to the chlorinated sample of water. This will dechlorine the sample and
orthodolidine is then added. The colour formed (Xl) now is only the intensity of colour
caused by interfering agents like nitrates, iron, manganese etc. Now another sample is
taken in another test tube and orthotolidine solution is added first, and just after 5
seconds, sodium arsenite is added. The sodium arsenite will arrest the colour to be
formed by the combined chlorine. Hence, the colour observed at the end of the
experiment (X2) will be due to the free residual plus due to the interfering colour causing
compounds of iron, manganese etc. Then, a third test is conducted on a third sample of
water. In this, only the orthotoldine solution is added to the given sample of water and
the colour is noted after 5 minutes. The noted colour (X3) will evidently be due to the
free and combined chloeine plus due to interfering colour causing copounds.
Now the different chlorine residuals can be easily determined as follows:
Free residual chlorine = (X2) (X1)
Combine residual chlorine = (X3) (X2)
In the orthotolidine test as well as in the OT A test, the temperature of water is to be
controlled to room temperature.
This test is widely used nowadays. The testing is carried out in a colour comparator, so
as to easily compare the developed colour with the standard colours. The comparator is
provided with a special dulling screen with a disc which must be fitted to the right hand
viewing window of comparator. The comparator discs will be available in two different
chlorine concentration ranges;
0.1 to 1.0 mg/L of chlorine and 0.2 to 4.0 mg/L of chlorine.
In order to carry out the test, 10 mL of water sample is taken in a tube, and it is placed
in the left compartment of the comparator. A reagent tablet (DPD chlorine tablets) is
placed in another tube to which 1 cm depth of water is added and the tablet is allowed
to dissolve. More water is now added to this until the tube contains 10 mL. Now the
comparator disc is rotated till the colours match. The residual chlorine amount can be
directly read from the window in the lower right corner of the instrument.
Chloroscope test
This is similar to DPD test with comparator instrument, called chloroscope. Orthotolidine
is added to the sample of water to produce colour and that colour is matched with
standard colours by rotating the disc through the viewing window.
20
Collect samples in amber, glass bottles treated with bleach to remove chlorine demand.
Treat bottles by filling with DI water, adding a few mL of household bleach, allow to
soak about 30 minutes and then rinse thoroughly with tap water followed by DI water.
Minimize the time between sampling and analysis (preferably = < 15 mins.). Warm
samples to room temperature before testing with the ISE (Ion Selective Electrode)
method. Fill sample completely to minimize contact with the air until samples are tested.
There are four main methods to test free and total chlorine residual in drinking water in
the field in developing countries: 1) Pool test kits; 2) Color-change test tubes; 3) Color-
wheel test kits; and, 4) Digital colorimeters. All four methods depend on a color change
21
to identify the presence of chlorine, and a measurement of the intensity of that color to
determine how much chlorine is present.
The first option for chlorine residual testing uses the liquid chemical OTO (othotolidine)
that, when added to water with total chlorine in it, causes a color change to yellow. To
complete the test, users simply fill a tube with water, add 1-5 drops of the solution, and
look for the color change to yellow. These kits are sold in many stores as a way to test
the concentration of total chlorine in swimming pool water. This method does not
measure free chlorine.
Benefits of the pool test kits are: Low cost Very easy to use easily purchasable
Drawbacks of the pool test kits are: Degradation of the OTO solution can cause
inaccurate readings over time generally not reliable quantitative results Lack of
calibration and standardization Measurement of total chlorine, not free chlorine
The Lamotte Company developed a rapid presence/absence test kit for free chlorine
residual in response to the 2004 tsunami in Indonesia. The test tube color comparator
uses DPD (N, N diethyl-p-phenylene diamine) tablets that causes a color change to pink
in the presence of chlorine. To use the kit, users add 5 mL of water to the test tube, add
one rapidly dissolving DPD-1R tablet, and compare the results to a color chart. This kit
combines the simplicity of the pool test kits, with the benefit of testing for free chlorine
residual instead of total chlorine residual. To use this kit to measure total chlorine, a
DPD-4R tablet can be added instead of the DPD-1R tablet to the water sample, or a
DPD-3R tablet can be added to the sample water after the DPD-1R tablet has been
added and the free chlorine residual has been read. Although the test kit is not
available as a package from Lamotte, the individual components (test tube, DPD
tablets, and color chart) can be ordered individually. Test kit instructions and part
numbers can be found at: http://www.lamotte.com/pages/common/pdf/instruct/3529.pdf.
Benefits of the test tube kit are: Low cost Very easy to use Measurement of free
chlorine
Drawbacks of the test tube kit are: generally not reliable quantitative results Lack of
calibration and stand
22
Color wheel test kits also use DPD tablets or powder that, when added to water with
free or total chlorine present in it, cause a color change to pink. The color wheel test
kits are more accurate than the pool test kits / test tube DPD color comparators and
simpler and less expensive than digital meters. This is because users measure the
intensity of the color change, as compared with a sample of water to which no DPD has
been added, using a color wheel to visually match the color change to a numerical
reading. The test kit can be used to measure either free chlorine or total chlorine, or
both, generally within a range of 0-3.5 mg/L, equivalent to 0-3.5 ppm (parts per million).
Please note that DPD tablets and powders are company specific, and that using one
companys test kit with another companys DPD tablets is not recommended. Also note
that although all DPD-1 tablets measure free chlorine, there is variation between
companies as to whether the total chlorine tablet is added to the same sample water the
free chlorine is tested in or whether it is added to fresh sample water.
Benefits of the color-wheel test kits are:
Accurate quantitative readings if used correctly Low cost
Drawbacks of the color-wheel test kits are:
Potential for user error Lack of calibration and standardization
4. Digital Colorimeters
Digital colorimeters are the most accurate way to measure free chlorine and/or total
chlorine residual in the field in developing countries. To use the colorimeters: 1) a
23
DPD-1 (free chlorine) or DPD-3 (total chlorine) tablet or powder is added to a vial of
sample water that causes a color change to pink; and, 2) the vial is inserted into a meter
that reads the intensity of the color change by emitting a wavelength of light and
automatically determining and displaying the color intensity (the free or total chlorine
residual) digitally. The range of the meters is generally 0-4 mg/L, equivalent to 0-4 ppm
(parts per million). Please note that DPD tablets and powders are company specific,
and that using one companys test kit with another companys DPD tablets is not
recommended.
Benefits of the digital colorimeters are: highly accurate readings Fast results EPA
approved
Drawbacks of the digital colorimeters are: Expense Necessity of calibration with
standards Necessity for skilled operator. The starch-iodide titration method, one of the
oldest methods for determining chlorine, is very non-specific for oxidants and generally
is used for total chlorine testing at levels above 1 mg/L CI2.
Chlorine will liberate free iodine from potassium iodide (Kl) solutions at pH 8 or less.
The liberated iodine is titrated with a standard solution of sodium thiosulphate
(Na2S203) with starch as the indicator.
This method is based on reaction with thiosulfate solution
The end point of the titration is indicated by the disappearance of the blue- colored,
starch-iodide complex.
MATERIALS REQUIRED
APPARATUS REQUIRED
1. Burette & Burette Stand
2. Porcelain Tile
3. Pipettes with elongated tips
4. Pipette Bulb
5. Wash Bottle
6. 250 mL Graduated Cylinder
7. 500 mL Conical Flask (Erlenmeyer flask)
CHEMICALS REQUIRED
1. Acetic Acid, Cone, (glacial)
2. Potassium Iodide, Kl, crystals
3. Sodium thiosulphate
4. Starch indicator
5. Distilled or Deionized Water
24
PROCEDURE CHART
Water
sample
Sodium thiosulphate
until the colour
turns straw yellow
_____
Starch indicator
25
Do not allow samples to freeze. Do not open sample bottle before analysis.
PRECAUTIONS
The following precautions should be observed while performing the experiment:
This experiment is a basic experiment and hence there will not be any major
difficulties in performing the experiment. The entire procedure should be done in quick
time without exposing the solutions to the ambient air.
Do not expose the potassium iodide crystals in the air. If possible do the
experiment in iodine flask instead of conical flask.
Chlorine in water solutions is not stable. As a result, its concentration in samples
decreases rapidly.
Samples to be analyzed for chlorine cannot be stored or preserved. Tests must
be started immediately after sampling. Therefore, samples taken for the chlorine
residual test must be grab samples only and excessive agitation must be avoided.
Exposure to sunlight or other strong light, air, or agitation will further reduce the
quantity of chlorine present in solutions.
PROCEDURE
1) lodometric Method: This method is more precise than colorimetric method where
residual concentration exceeds 1mg/L, but for lower concentration it is not so accurate.
2) Stabilized Neutral Ortho-Toluidine method: This method is useful to determine
free available chlorine and combine chlorine. This method is sensitive to low residual
chlorine concentrations.
PREPARATION OF REAGENTS Sodium Thiosulphate solution (0.01 N)
TESTING OF SAMPLE
Rinse the burette with sodium thiosulphate and then fill the burette with sodium
thiosulphate.
Add about 1 g Potassium Iodide (Kl) measured using the spatula and dissolve it
by thoroughly mixing it with stirring rod.
Add 1 mL of starch solution and continue the titration until the blue color
disappears.
In many cases residual chlorine is very low and starch needs to be added before
starting up the titration.
Note down the burette reading (to know the volume of sodium thiosulphate
added).
Chlorine Demand: The amount of chlorine used by reactions with substances that
oxidize in the water before chlorine residual can be measured. It is the difference
between the amount of chlorine added to wastewater and the amount of chlorine
residual remaining after a given contact time. Chlorine demand may change with
dosage, time, temperature, pH, and the type and amount of pollutants in the water.
Chlorine Dosage: The amount of chlorine which must be added to produce a desired
result (disinfection of the effluent, control of filter flies, ponding and odor).
given contact time (20 minutes at peak flow; 30 minutes at average flow), and under
specific conditions including pH and temperature.
Free Available Chlorine Residual: The residual consisting of hypochlorite ions (OCl-),
hypochlorous acid (HOCl) or a combination of the two. These are the most effective in
killing bacteria.
Total Chlorine Residual: The total amount of chlorine present in a sample. This is the
sum of the free chlorine residual and the combined available chlorine residual.
CALCULATIONS
EXAMPLE:
mL PAO added = 5 mL
mL iodine titrated = 0.5 mL
mL sample = 200
mg/L Cl2 = [5 - (5 x 0.5)] x 200/200 = [(5 - 2.5) x 200]/200
mg/L Cl2 = (2.5 x 200)/200 = 2.5
The amount of iodine used for the titration can be corrected for a non-standard iodine solution
normality by applying a correction factor based on the actual iodine normality.
mg/L Cl2 = [mL PAO added - (5 x mL iodine titrated x CF)] x 200/mL sample
CF = Correction Factor = Actual normality of iodine/0.0282 N
EXAMPLE: