Beruflich Dokumente
Kultur Dokumente
discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/304354526
CITATIONS READS
0 230
4 authors, including:
S.I.R. Okoduwa
Nigerian Institute of Leather Science and Technology
18 PUBLICATIONS 15 CITATIONS
SEE PROFILE
Some of the authors of this publication are also working on these related projects:
Appropriate Insulin Level for selecting animal model of Type 2 Diabetes View project
All content following this page was uploaded by S.I.R. Okoduwa on 26 June 2016.
The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document
and are linked to publications on ResearchGate, letting you access and read them immediately.
A Publication Series By
ABSTRACT
Saccharin, one of the sweeteners in the world, is still regarded as a carcinogen and diabetic inducer in some
parts of the world. Concern peaked in 1977, after publication of a study indicating an increased rate of bladder
cancer in rats fed large doses of saccharin. In 1977, Canada banned saccharin while US-FDA also proposed a
ban. In due course, US congress required all saccharin-containing foods to display a warning label indicating
that saccharin may be carcinogenic. This resulted in the carcinogenicity, genotoxicity, hepatotoxicity and
teratological studies of saccharin in animals including humans by the most highly reputable global health and
credible science organizations worldwide. None of these studies ever showed a clear causal relationship between
saccharin consumption and health risks in humans at normal dosage. Ultimately, the influential 1977 study was
later criticized for the high dosages of saccharin that were given to test subject rats. Consequently, the US-FDA
formally withdraws its 1977 proposal to ban the use of saccharin and the National Toxicity Program announced
the delisting of saccharin as a carcinogen. Therefore, use of saccharin can bring about a healthy lifestyle free of
calorie accumulation and the risk of obesity with its associated cardiovascular complications.
Since it is not metabolized in the body for energy, saccharin of nectar, do not treat it as a pleasing substance. [2]. The
is classified as a non-caloric sweetener. By the 1960s it was figure 2 below shows the sweetness receptor with saccharin.
used on a massive scale in the "diet" soft drink industry [2].
Consequent upon sugar shortage during the World War I,
saccharin became widespread and commercialized. Since
saccharin is a calorie-free sweetener, its popularity further
increased during the 1960s and 1970s among dieters [2]. In
the United States, saccharin is often found in restaurants in
pink packets; the most popular brand is "Sweet 'N Low".
Saccharin is used to sweeten products such as drinks,
candies, medicines, and toothpaste, canned fruit, jams, salad
dressing, chewing gum, table top sweeteners, baked goods,
jams, and dessert toppings etc [2]
studies of saccharin used a two-generation model, as at the a 37.9% incidence of lens anomalies versus 12.4% incidence
time of Arnolds publication. These studies categorically for the control animals [25].
demonstrated that when rats were exposed to diets Genotoxicity Study: Several in-vitro and in vivo studies
containing 5% or 7.5% saccharin from the time of have shown clastogenicity, specifically at high
conception to death, an increased frequency of urinary concentrations in in-vitro studies [26, 27]. In several in-vitro
bladder cancers was found, mostly in males. There was an studies for induction of chromosomal aberrations in Chinese
observation of the fact that saccharin is not metabolized, it is hamster cells and in human lymphocytes, sodium saccharin
nucleophilic and does not bind to DNA. But, it does suppress was found weakly positive [27, 28]. By and large weak
humoral antibody production in rats. At dosages of 5% or responses were observed in some in-vitro assays at the
greater, saccharin does not act as a typical chemical chromosomal level. However, these were only seen in high
carcinogen, based on the theory that all carcinogens are concentrations and it is possible that they are attributable to
strong electrophilic agents [4]. The finding from the study ionic imbalances which are known to cause non-specific
above lead to the prohibition of saccharin in Canada and a effects. There are also conflicting reports from in vitro
proposed ban in the United States [6, 19]. studies, but some cases the materials use was found or
known to contain impurities or contaminants from the
In 1991, the U.S. withdrawn her proposed ban, but foods manufacture of saccharin [29].
containing saccharin were required to carry a warning label
Epidemiological Study: Series of evidence comes from the
[20, 19]. To indicate that saccharin is a potential cancer
now numerous epidemiological studies on saccharin which
causing agent, a warning label was placed on all products
have included studies of groups consuming relatively high
containing saccharin. Current research showing the safety of
this product led to this decision being overturned in 2000 levels of saccharin. In a reviews by Chappel, [30]; Elock and
[21]. Though, a ban on saccharin still exists in Canada, Morgan, [31], it was indicated that there is no detectable
association between artificial sweetener consumption most in
having considering the fact that series of toxicological
particular saccharin and bladder cancer in humans. Various
evidence and the lack of a consistent association in
epidemiological studies indicates no increase in the
epidemiological studies the Health Canada suggests that
occurrence of bladder tumours in human from the ingestion
carcinogenic effects of saccharin noted in rats are not
of saccharin, including in individuals with the highest
relevant to humans. Hence, they are considering re-listing
consumption rate of artificial sweetened beverages and those
saccharin as a food additive in the Canadian Food and Drug
using saccharin as a table-top sweetener.
Regulations for use as a sweetener in the proposed food
categories [22, 19].
Beneficial Usage of Saccharin: All over the world, Food
Hepatotoxicity Study: In 1992, Kumar, et al. reported that and beverages industries have over the century found the use
saccharin posed no threat to liver function [23]. It was also of saccharin imperative due to its absence of carbohydrate
reported that in a patients who had elevated serum and no calorie value. For example, in Europe, the use of
concentrations of liver enzymes after the oral administration saccharin became more considerable after the two world
of three different drugs, of which saccharin was the only wars. Several generations of Americans has made the use
common constituents, re-exposure to pure saccharin saccharin an integral part of their daily lifestyle in the United
supported its role in the pathogenesis of liver damage in the State. Most in particular, the diabetic individuals whose diets
patients. The pathogenesis of saccharin hepatotoxicity in require a restriction of caloric or carbohydrate intake. A
these patients is unclear. Symptoms suggestive of good number of health practitioners support the use of a non-
hypersensitivity were absent. Saccharin is not metabolized in caloric sweetener like saccharin in weight reduction and for
vivo, being in an almost unmodified form in the urine, and it people with diabetes [21].
does not accumulate in the liver. The small amount of
saccharin (never exceeding 16 mg daily) taken by patients According to the Calorie Control Council Research (CCC),
underscores the idiosyncratic nature of the reaction [23, 24]. Health professionals believe saccharin is especially
beneficial to persons with diabetes and the obese, and helps
Teratology Study: Till date, the teratogenic study of reduce dental cavities. According to opinion research, people
saccharin with mice has always been negative. In an use saccharin to stay in better overall health, control weight
experiment conducted on feeding pregnant female rats with or maintain an attractive physical appearance. In another
diets containing 0.3% saccharin throughout the gestation report of the CCC, No low-calorie sweetener is perfect for
period shown that the pups from saccharin treated dams had all uses. But, a range of sweeteners enables the development
of a much wider range of new, good-tasting, low-calorie
products to meet consumer demand. Also, an array of low- "Epidemiological studies have also not established any
calorie sweeteners provides products with increased stability, evidence that bladder cancer in man is associated with
improved taste, lower production costs and more choices for saccharin intake [33, 34].
the consumer [21].
CONCLUSION
Saccharin is important for a wide range of low-calorie and The Joint Expert Committee on Food Additives (JECFA) of
sugar-free food and beverage applications. It is used in such the World Health Organization and the Scientific Committee
products like soft drinks, tabletop sweeteners, baked goods, for Food of the European Union has reviewed and certified
jams, chewing gum, canned fruit, candy, dessert toppings the safety of saccharin. As of today, saccharin is approved in
and salad dressings. It is also used in cosmetic products, more than 100 countries around the world. It can therefore
vitamins and pharmaceuticals. One of the most popular uses be recommended as one of the very best choice for diabetic
of saccharin is in the production of the product called Sweet patients and those dieting. Therefore, use of saccharin can
'N Low, a tabletop sweetener in the United State [21,30]. bring about a healthy lifestyle free of calorie accumulation
and the risk of obesity with its associated cardiovascular
GLOBAL REPORTS ON THE SAFETY OF complications.
SACCHARIN
Over the years, series of researchers, corporate bodies and REFERENCES
individuals have worked on saccharin. The most highly
reputable global health and credible science organizations 1. Ahmed Z, Banu H, Akhter F, Faruquzzaman KM and
have these comments to say with respect to the evaluation Haque S. Concept of sugar-a review. Online Journal of
and confirmation on the safety of saccharin as it relate to Biological Science 2001, 1(9): 883-894
human subjects.
2. Ophardt CE. Saccharin - the oldest Sweetener Sweet 'N
Low, Sugar Twin. V.Chmbook 2003, 549
The National Cancer Institute in its "Cancer Facts"
documents reviewed in 2009 stated that epidemiological 3. Anderson J and Young L. Sugar and sweeteners. Health,
studies do not provide clear evidence of saccharins link to Food and Nutrition series. 2008, 9: 301
human cancer.
4. National Cancer Institute, NCI. Artificial sweeteners
The World Cancer Research Fund Stated in the American and Cancer. Fact Sheet. Rev. 2009, 3:19
Institute for Cancer Research 2007 reported on page 143 that
5. Remsen I and Fahlberg C. "ber die Oxydation des
the evidence from epidemiological studies does not suggest
Orthotoluolsulfamids". Chemische Berichte. 1879, 12:
that artificial sweeteners have a detectable effect on the risk 469473.
of any cancer [32].
6. Arnold DL. Toxicology of saccharin. Fundamental and
The American Dietetic Association "Use of Nutritive and Applied Toxicology, 1984, 4(5): 674-685.
Non-nutritive Sweeteners" position statement, on July 1993.
States that "Evidence gathered from the numerous animal 7. Whitehouse CR, Boullata J and McCauley LA. The
Potential Toxicity of Artificial Sweeteners. AAOHN
and human studies of saccharin does not suggest that there is
Journal, 2008, 56(6): 251-259.
any significant risk to the human population from the normal
use of saccharin. 8. Priebem PM and Kauffman GB. Making governmental
policy under conditions of scientific uncertainty: A
Members of the British Medical Association Advised in century of controversy about saccharin in congress and
the British Medical Journal, that "The major benefits of the laboratory". Minerva, 1980, 18 (4): 556574
saccharin are; an improved quality of life, low cost, and
9. Ager DJ, Pantaleone DP, Henderson SA, Katritzky AR.,
stability at warm temperatures. A small risk for bladder Prakash I and Walters DE. Commercial, Synthetic
cancer continues to be found in male rats exposed to high Nonnutritive Sweeteners. Angewandte Chemie
doses of saccharin. However, epidemiologic studies show no International Edition 1998, 37 (13-24): 18021817
evidence of a carcinogenic effect in man [33].
10. Bungard, G. Die SusStoff Der deut Apotheker, 1967,
The Health Protection Branch of the Health and Welfare 15:150
Canada, on December 5, 1991 declared that
17. Weihrauch MR and Diehl V. Artificial sweetener-do 30. Chapel CL. A review and biological risk assessment of
they bear a carcinogenic risk?. Annals of Oncology., sodium saccharin. Regulatory Toxicology and
2004, 15 (10): 1460-1465 Pharmacology., 1992, 15: 253-270
18. Henkel, J. Sugar Substitutes: Americans Opt for 31. Elcock M and Morgan RW. Update on artificial
Sweetness and Lite, FDA Consumer, Revised, sweeteners and bladder cancer. Regulatory Toxicology
2006, 6:3 and Pharmacology., 1993, 17: 35-43
19. Misner S, Curtis C and Whitmer E. Sugar substitute-Are 32. Commission of the European Communities (CEC).
they safe? Arizona Cooperative Extension, 2008, Report of the Scientific communities for food on
AZ:1229 saccharin. Reports of the scientific committee for food,
Luxembourg, 4th series. 1977, Pp 7-23
20. ISA, International Sweeteners Association ISA. Fact
sheet on low calorie sweeteners. 2008, Retrieved from 33. Noah L and Merrill RA. Starting From Scratch?:
http://www.isabru.org/EN/about_sweeteners_factsheet.a Reinventing the Food Additive Approval Process,
sp 78 B.U. L. Rev.1998, 329, 336-401.
21. CCC. Calorie Control Council. Low calorie sweeteners. 34. International Food Information Council Foundation,
Saccharin, 2007. Retrieved from http:// IFICF. Facts about low-calorie sweeteners. Food
www.caloriecontrol.org/saccharin.html Ingredients. online: www.foodinsight.org, 2009.
This article is a postgraduate research seminar presented by the first author to the Department of Biochemistry, Ahmadu Bello University, Zaria-
Nigeria, in partial fulfillment for the assessment of Nutritional and Environmental Toxicology in 2010. Infohealth Awareness Article is a Copyright of
InfoHealth Awareness Group, a Non-Profit Organisation aimed at eradicating preventable diseases and hereditary disorders in Nigeria in order to have
a Society of people living a healthier life. 2013 Infohealth Awareness Article. Alrights Reserved, Published By SIRONigeria Global Limited.