Cell Culture Solutions
Updated 6/15/07 JEB
Borate Buffer (4)*
4.76 g Boric Acid (Cat # B-1934)
2.54 g Borax (Cat # B-0127)
1000 mL of ddH2O
pH to 8.4 with NaOH
Sterile Filter
Plating Media (4)
40 mL F12 (Cat # N8641)
40 mL FBS (Cat # sh30070.03)
420 mL Dulbecco Modified Eagle
Medium (DMEM) W/O L-Glutamine
(Cat # 11960)
1 mL of PS (Cat # 17-602E)
200 uL of 200 mM L-Glutamine
(Cat # G7513)
D2C (4)
10 mL FBS (Cat # sh30070.03)
1 mL PS (Cat # 17-602E)
12.5 mL of 1M Hepes (Cat # H0887)
471.5 mL Dulbecco Modified Eagle
Medium (DMEM) W/O L-Glutamine
(Cat # 11960)
6.25 mL of 200 mM L-Glutamine (Cat
# G7513)
Neurobasal (4)
1 bottle (500 mL) Neurobasal Media
(Cat # 21103)
1 bottle (10 mL) 50X B27(Cat #
17504-044)
P/S (Cat # 17-602E)
Stock Ara-C (500X) (-20)*
100 mg Ara-C (Cat # C-6645)
6 mL sterile H2O
Sterile Filter
Aliquot 0.5 mL
*=good for months, =good for ~1 week, = make fresh
Note: We are no longer heat inactivating our Fetal Bovine Serum
BB 9/15/05
Working Ara-C (4)*
20L Stock
10 mL sterile T.C. water
10% Formaldehyde (RT or 4)*
5 mL of 37% Stock Solution
45 mL of PBS
Glucose Free Balanced Salt
Solution*
5.26 g NaCl (Cat # S9625-5KG)
0.125 g KCl (Cat # P-9541)
66.6 mg CaCl2 (Cat # C-4901)
1.43 g Hepes (Cat # H-4034)
600 mL ddH2O
Sterile Filter
pH 7.3
PLO
1 mg PLO : 2 mL Borate Buffer (Cat #
P3655)
Sterile filter
Make up fresh each time it is used
HBSS (4)*
8.4 g NaCl (Cat # S9625-5KG)
224 mg KCl (Cat # P-9541)
2.38 g Hepes (Cat # H-4034)
1.0 g Glucose (Cat # G-5250)
1000 mL Milli Q
pH to 7.3 with NaOH
Sterile Filter
Roccal II (10%) (RT)*
10% Benzalkonium Chloride (Cat #
234427 Sigma)
1.25% Ethyl Alcohol
Dissolve in alcohol with stirring
 Cell Culture Solutions BB 9/15/05
Updated 6/15/07 JEB
Add 88.75% Milli Q water

MEM/BSA/Hepes
487.5 mL of clear MEM (Cat # 51200)
12.5 mL of 1 M Hepes (Cat # H0887)
50 mg of BSA (Cat # A7888)
Sterile Filter

*=good for months, =good for ~1 week, = make fresh

Note: We are no longer heat inactivating our Fetal Bovine Serum