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Gram negative
Mesophiles
Facultative anaerobs
consist of 5 type
Tribe I : Escherichieae
Tribe II : Klebsiellea
Tribe III : Proteae
Tribe IV : Yersiniae
Tribe V : Erwineae
1.Salmonella genus:it's characteristic. Latin name of enteric fever pathogens. Their morphology.
Culture properties, factors of pathogenicity.
Classification of Salmonella genus
Salmonella genus classified into 2 species
1) S.enterica - has been divided into 6 subspecies.(serotypes)
- cause infection in men and animals.
2) S.bongori - has been subdivided into6 subspecies. (serotypes)
Cause infection in animals only.
Latin name :: Salmonella enterica
Morphology
Salmonella morphologically short, gram negative rods, non-sporing, non-capsulated but flagellated.
These microorganism are mesophiles (37 temperature growth), facultative anaerobs, can grow in
simple media.
Cultural properties
Salmonella can grow in MPA / MPB
Selective media to cultivate Salmonella are:
Bile broth (liquid medium)
MacConkey media
Salmonella-Shigella agar (SSA)
Eosin Methylene Blue (EMB)
Bismuth-containing agar
Rapaports medium (this medium consist of 10%bile broth, 1%glucose, indicator)
Factor of virulence
Salmonella possess following antigens: O, H, Vi antigens.
2.Describe microbiological diagnosis of bacillary dysentery (day by day)
V.6
Family : Enterobacteriaceae
Genus : Shigella
Species: S. dysenteriae
S. flexneri
S. boydii
S. Sonnei
Shiga-like toxin is released by Shigella species which have not shiga toxin( all except S. dysenteriae
Type 1). It causes cytotoxic action in cultured cells but action is not as powerful as Shiga toxins.
Endotoxin causes common intoxication and stimulates motility of the intestine.
Campylobacter are slender, spirally curved, Gram (-) rods. They have typically coma shaped but
may occur as letter S or multi spiral chains. In old cultures, Campylobacters are pleomorphic in
shape.
They are non-sporing, motile, and non-capsulated.
Growth occurs under micoaerophilic conditions. Only 5% of O2 is enough for optimal growth.
Campylobacter do not grow in simple media. They grow only in complicated media with blood,
amino acids, salts, gemine and protein hydrolasate. Optimum pH=7.0.
For example: campi BAP medium.
They cant destroy carbohydrates, oxidase and catalase tests are positive, H2S is formed.
2.Salmonella food poisoning:specimen, medical diagnosis, serological investigation, nutrient media,
classification of pathogen.
Factor of virulence
Liable toxin (LT)
Stable toxin (ST)
Shigella toxin (SLT)
Endotoxin
Clinical manifestation
1) Abdominal pain
2) Diarrhea
3) Vomiting
4) Fever
Prophylaxis
There is no specific prevention, no vaccine. Only by following hygiene procedures and proper
cooking of food.
Treatment
Evacuation of all food from GIT
Drink KMnO4 (in order to absorb toxin, neutralize toxin and avoid vomiting)
V.10
1.Antigenic structure of Salmonella. Localisation og antigens in bacterial cell
Antigenic structure of Salmonella
Salmonella possess following antigens: O, H, Vi antigens.
Antigen
Serological group Name H
O
Phase 1 Phase 2
A S.paratyphi A 1, 2, 12 a -
S.derbi 1, 4, 5, 12 f, g 1, 2
S.haifa 1, 4, (5), 12 Z10 1, 2
B
S.paratyphi B 1, 4, 5, 12 b 1, 2
S.typhimurium 1, 4, 5, 12 i 1, 2
S.infants 6, 7 R 1, 5
C1 S.choleraesuis 6, 7 c 1, 5
S.virchov 6, 7 R 1, 5
C2 S.newport 6, 8 eh 1, 2
S.dublin 1, 9, 12(vi) g, p -
S.enteritidis 1, 9, 12 g, m -
D
S.panama 1, 9, 12 e, v 1, 5
S.typhi 9, 12(vi) d -
E1 S.anatum 3, 10 ch 1, 6
2.Complete cholera diagnosis (step by step)