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Description

Designation:FDA209
DepositedName:Staphylococcusaureussubsp.aureusRosenbach
ProductSheet ProductDescription:Usedintestingmedia,sterility,sanitizers,disinfectants,antimicrobialpreservatives,
bacterialresistanceincarpetsandtextiles,andasacontrolstrainforBiosynthproducts.Thisstrainis
Staphylococcusaureus recommendedbyATCCforuseinthetestsdescribedinmilitaryspecificationMILG13734Bwhereonlythe

subsp.aureus(ATCC taxonisspecified.

6538) Propagation

Medium
ATCCMedium18:TrypticaseSoyAgar/Broth
PleasereadthisFIRST
GrowthConditions
StorageTemp. Temperature:37C
Frozen:80Cor Atmosphere:Aerobic
colder
PropagationProcedure

FreezeDried:2C 1. Openvialaccordingtoenclosedinstructions.
to8C 2. Usingasingletubeof#18broth(5to6mL),withdrawapproximately0.5to1.0mLwithaPasteuror
LiveCulture:See 1.0mLpipette.Rehydratetheentirepellet.
Propagation 3. Asepticallytransferthisaliquotbackintothebrothtube.Mixwell.
Section 4. Useseveraldropsofthesuspensiontoinoculatea#18agarslantand/orplate.
5. Incubatethetubesandplateat37Cfor24hours.


BiosafetyLevel Notes
2
ThisstrainisalsoavailableascertifiedreferencematerialATCCCRM6538.
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.

IntendedUse
References
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.
diagnosticuse.
BiosafetyLevel:2
CitationofStrain
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
Ifuseofthiscultureresultsinascientificpublication,it thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
shouldbecitedinthatmanuscriptinthefollowing DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
manner:Staphylococcusaureussubsp.aureus forHealth.
(ATCC6538)
ATCCWarranty

ATCCproductsarewarrantedfor30daysfromthedateofshipment,andthiswarrantyisvalidonlyifthe
productisstoredandhandledaccordingtotheinformationincludedonthisproductinformationsheet.Ifthe
ATCCproductisalivingcellormicroorganism,ATCCliststhemediaformulationthathasbeenfoundtobe
effectiveforthisproduct.Whileother,unspecifiedmediamayalsoproducesatisfactoryresults,achangein
mediaortheabsenceofanadditivefromtheATCCrecommendedmediamayaffectrecovery,growthand/or
functionofthisproduct.Ifanalternativemediumformulationisused,theATCCwarrantyforviabilityisno
longervalid.

Disclaimers

AmericanTypeCultureCollection Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
POBox1549 WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproductsheet,
Manassas,VA20108USA ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureand
www.atcc.org
patentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeen
confirmedtobeaccurate.
800.638.6597or703.365.2700
Fax:703.365.2750 Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.ATCC
Email:Tech@atcc.org isnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.Whilereasonableeffort
ismadetoinsureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfrom
Orcontactyourlocaldistributor
themisidentificationormisrepresentationofsuchmaterials.

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PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2017.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[01/12]

ProductSheet
Staphylococcusaureus
subsp.aureus(ATCC
6538)

PleasereadthisFIRST

StorageTemp.
Frozen:80Cor
colder

FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section


BiosafetyLevel
2

IntendedUse

Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain

Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Staphylococcusaureussubsp.aureus
(ATCC6538)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org

800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

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