CHAPTER 3

METHODOLOGY

Research design

Approximately 50 freshwater fish samples (tilapia, gourami, mudfish, African catfish,

and pupuyo) were collected from fish stalls in Midsayap public market for the detection of
endoparasites and ectoparasites. Sampling will done depending on the availability of fish until
the total number of fish needed was met. The samples were placed in a styrofoam and plastic
bags with water

Locale of the study

This study will be conducted at Midsayap,Cotabato public market fish stalls

Research Instrument

Dissecting pans, magnifying glass, tissue forceps and a microscope will be needed to
examine the parasites.

Data gathering

The experimental fish was placed in dissecting pans. The scales were scraped and
examined with the use of magnifying glass, for the presence of parasites. The gills, intestines,
and other internal organs were also examined for the presence of parasites. A tissue forceps was
used to expose the underlying tissue rested. A macroscopic examination was made to detect the
presence of parasites.