Chemistry 315: Experiment 9 Mass Spectrometry of Biomolecules

Student Name:

_____ / 2 Title Page

-Proper template format (1 pt)
-Abstract and keywords (1 pt)

_____ / 8 Introduction
-Background on MS in general and ESI-Ion Trap (3 pts)
-Describe MS/MS (2 pt)
-Bradykinin background (2 pt)
-Clear, concise, 3rd person (1 pt)

_____ / 5 Experimental
-Concise explanation of lab procedure (3.5 pts)
-Identify instrument make and model (1 pt)
-Cite lab manual (0.5 pt)

_____ / 27 Data and Results and Discussion*

-Answer questions 1-4 (2 pts per letter)
1a) Calculate MH+, (MH+1)+, (MH+2)+
1b) Compare above with spectra
2a) Describe fragmentation
2b) Discussion of fragments seen in spectra
2c) Table of fragments and assignments
3a) Identify unknown and explain approach
3b) Table of unknown peaks with abundance, note if resolved
3c) Table of unknowns theoretical m/z peaks, m, and % error
3d) Calculate unknown molecular mass, m, and % error
4a) Compare theoretical and observed bradykinin charge states. Calculate m/z, % error
4b) Calculate and discuss full scan vs. zoom scan resolution
4c) Identify and discuss peaks with S/N > 3
-Answer Chemical Engineering Question if applicable (1 pt)
-Original spectra and lab notebook copies included in supplementary (2 pts)

_____ / 3 References
-Proper Format
-Minimum 3 total (lab manual, required reading, post lab questions)

_____ / 5 Format, grammar, punctuation etc.

_____ / 0 Deductions (in lab)

_____ / 50 TOTAL

*The Data and Results section should be a separate from Discussion section in your report. They are only
combined above to simplify grading. You need to decide whether a question should be in Data or Discussion.
One way to determine where to answer a question: does it ask for a figure (Data and Results) or does it ask
for an interpretation (Discussion).