Chart 1.

Test/Medium Organism Color of Color of inoculated medium Interpretation

uinoculated and colony
medium
Mannitol Salt Staphylococcus aureus Magenta/Red Yellow/Off white colonies and S. aureus is a positive mannitol fermenter
Agar (MSA) (mostly for pathogenic) yellowish zones on orange medium given the results of yellow colonies and
yellow zones on the medium.
Transparent colonies and red-
orange medium M. luteus is a negative mannitol
Micrococcus luteus Magenta/Red fermenter given the results of transparent
colonies and its retention of the mediums
redness.

MacConkey Klebsiella oxytoca Magenta Pink and white colonies, red violet K. Oxytoca is a lactose fermenter. Also a
Agar (MCA) medium gram-negative

Pseudomonias aeroginosa Magenta Pink growth, orange medium Pseudomonias is a lactose fermenter.
Also a gram-negative.

Lysine Iron Agar Pseudomonias aeruginosa Violet Purple slant, purple deep Alkaline

Micrococcus luteus Violet Yellow deep, violet slant, white Acid

colony

Simmon citrate Enterococcus faecalis Green Green slant/deep, no growth E. faecalis is a negative
agar
Green slant/deep, no growth
Micrococcus luteus Green M. luteus is a negative
Blue slant/green deep, no growth

Serratia marcescens Green S. marcescens is possibly positive with

the given results of a blue slant, a change
in color of the medium

Triple Sugar Iron Pseudomonias aeruginosa Red White colony, red slant, red deep The organisms can only utilize peptone
Agar (TSIA) aerobically
Serratia marcescens Red White colony, red slant, yellow Organisms are non-lactose fermenters
deep
Micrococcus luteus
Red White colony, red slant, yellow Organisms are non-lactose fermenters
deep
Chart 2.

Medium Organism Name of test Results Interpretation

NBG Escherichia coli Methyl Red (MR) Negative Both have no acid production
because of the yellow
NBG Serratia marcescens Methyl Red (MR) Negative colorization

NBG Escherichia coli Voges-Proskauer (VP) Postive The organisms have

metabolized pyruvate further
NBG Serratia marcescens Voges-Proskauer (VP) Positive to neutral compounds

Chart 3.