Name: Satish Paul

Student ID: 0332610

Practical Session: 01

Principle of Life Sciences

Practical 4
Analysis of DNA Fragments using Agarose Gel Electrophoresis

1)

Figure 1: Image of Agarose Gel Electrophoresis set with DNA Ladder, Sample A and Sample B
stained with GelRed taken under a UV-Transilluminator.
(2)

Band Base Pairs Migrated Distance Log (Base Pairs)

(cm)
1 10000 2.1 4
2 7000 2.3 3.845
3 6200 2.5 3.792
4 5200 2.7 3.716
5 4000 3 3.602
6 3000 3.2 3.477
7 2500 3.5 3.398
8 2000 4.1 3.301
9 1500 4.9 3.176

Table 1: Table of the migrated distance (cm), base pairs and log (base pairs) of the 7 bands of of
the DNA Ladder

Band Migrated Distance (cm) Log (Base Pairs) Base Pairs

1 1.9 3.9517 8947
2 2 3.9226 8368
3 2.2 3.8644 7318
4 2.6 3.7481 5599
5 3.9 3.3699 2344
6 4.1 3.3117 2050

Table 2: Table of the migrated distance (cm), base pairs and log (base pairs) of the 7 bands of
Sample B
(3)

4.5

3.5

3
Log( Base Pairs)

2.5

2 y = -0.2909x + 4.5044
R = 0.9341
1.5

0.5

0
0 1 2 3 4 5 6
Migrated Distance (cm)

3.9

3.8
Log( Base Pairs)

3.7

3.6

3.5
y = -0.2909x + 4.5044
R = 1
3.4

3.3

3.2
0 0.5 1 1.5 2 2.5 3 3.5 4 4.5
MIgrated Distance (cm)
(4)
Using the trend line equation, y = -0.2909x + 4.5044, from the scatter chart of the Migrated
Distance (cm) against log ( Base Pairs) of the DNA Ladder, the Value of y or log (Base Pairs)
can be obtained. The unknown x is the independent variable, Migrated Distance (cm).

Band 1 : y = -0.2909 (1.9) + 4.5044

y = 3.9517

log (Base Pairs) = 3.9517

Base pairs = Log^-1 (3.9517) = 8947 Base Pairs

Band 2 : y = -0.2909 (2) + 4.5044

y = 3.9226

log (Base Pairs) = 3.9226

Base pairs = Log^-1 (3.9226) = 8368 Base Pairs

Band 3 : y = -0.2909 (2.2) + 4.5044

y = 3.8644

log (Base Pairs) = 3.8644

Base pairs = Log^-1 (3.8644) = 7318 Base Pairs

Band 4 : y = -0.2909 (2.6) + 4.5044

y = 3.7481

log (Base Pairs) = 3.7481

 Base pairs = Log^-1 (3.7481) = 5599 Base Pairs
Band 5 : y = -0.2909 (3.9) + 4.5044

y = 3.3699

log (Base Pairs) = 3.3699

Base pairs = Log^-1 (3.3699) = 2344 Base Pairs

Band 6 : y = -0.2909 (4.1) + 4.5044

y = 3.3117

log (Base Pairs) = 3.3117

Base pairs = Log^-1 (3.3117) = 2050 Base Pairs