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Macroglobulinemia
Pernicious anemia
Multiple myeloma
Amyloidosis
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Urine chemical reagent strips readers can utilize any manufacturers dipsticks.
True
False
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This is not true. Each manufacturer will specify which urine chemical reagent strips
are acceptable for use on their instrumentation.
Urethra
Glomerulus
Loop of Henle
Proximal convoluted tubule
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The nephron, the basic functional unit of the kidney, consists of a glomerulus, the
proximal convoluted tubule, the loop of Henle, the distal convoluted tubule, and the
excretory duct.
Match the following reagent strip tests to the disease or disorder that would most
likely cause a positive test result.
Your answers are on the left. The correct answers are on the right and highlighted.
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bilirubin- Hepatitis/cirrhosis
WBC cast
RBC cast
Waxy cast
Granular cast
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RBC casts may appear as brown to almost colorless. Red cells can clearly be seen
within the cast. This type of cast is diagnostic of glomerular disease or intrarenal
bleeding.
red cell
white cell
waxy
fatty
granular
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White cell casts are closely associated with acute pyelonephritis since white blood
cells are drawn to the infection and inflammation of the kidney (definition of
pyelonephritis) and are encased into casts which slough off into the urine. Red cell
casts are indicative of glomerular damage and can be seen in various conditions, but
most often nephritic syndromes. Fatty casts are most commonly seen in nephrotic
syndromes. Granular casts, composed of broken down cells or immunoglobulins, are
most commonly associated with chronic renal disease.
Which of the following cells when found upon microscopic examination of the urine
would be most indicative of kidney disease:
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Tubular epithelial cells are frequently seen in patients with acute renal tubular
necrosis. They are most easily recognized when they form casts.
Cholesterol crystals
Uric acid crystals
Amorphous urate crystals
Cystine crystals
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viral meningitis
allergic reaction
cerebral hemorrhage
metastasis
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The nucleus in this cell is abnormal and appears to have divided. This finding
correlates with the presence of metastasis in the CSF.
Waxy cast
Coarse granular cast
Renal tubular epithelial cast
Fine granular cast
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Renal tubular epithelial casts are caused by the sloughing of necrotic renal tubular
cells. They can be due to allograft rejection, acute tubular necrosis, and certain drugs.
Waxy casts
Coarse granular casts
Renal tubular epithelial casts
Fine granular casts
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Waxy casts appear smooth and homogeneous, and highly refractile. They are present
in patients with severe renal failure, and can be due to allograft rejection.
Unconjugated bilirubin is conjugated in the liver with which of the following substances to
become water soluble?
Glycogen
Glucose oxidase
Glucuronic acid
Glycine
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Which of the following is true about Bence-Jones protein when performing the heat
precipitation test?
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Match urine color with substance that might have been responsible:
Your answers are on the left. The correct answers are on the right and highlighted.
Phosphates White
Bilirubin Yellow
Pseudomonas Blue to green
Porphobilinogen Red to brown
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Green:
1. Food colorings
2. Increased carotene in the diet
3. Pseudomonas aeruginosa infection
Neutrophils
Eosinophils
Basophils
Lymphocytes
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The presence of lymphocytes will not produce a positive leukocyte esterase test since
lymphocytes do not contain leukocyte esterase.
Which of the following statements are true regarding the storage and handling of
urinalysis chemical reagent strips? (Choose ALL correct answers)
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Out of the options listed, the correct answers are to store reagent strips at the
temperature recommended by the manufacturer of the strips and to remove only
enough strips for immediate use. It is never recommended to touch any of the reagent
pad areas or to leave the reagent strip bottle uncovered, as both can lead to inaccurate
results and faulty reagent strip analysis.
Cholesterol crystals
Uric acid crystals
Amorphous urate crystals
Cystine crystals
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Uric acid crystals are variably shaped: they occur as flat diamond (four-sized),
rhombic, lemon-shaped, or rarely hexagonal shapes with a yellowish or yellowish
brown coloration. They are present in acid urine. They are polarizable and show
various colors when polarized. They may be present in normal urine.
The patient is a female and the urinalysis is completed within two hours of collection.
True or false? The results are abnormal but all results correlate.
True
False
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Which of the following substances can cause a false positive result for blood on the
urine chemical reagent strip?
Ascorbic acid
Bleach in the collecting bottle
High levels of nitrite
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The presence of bleach in the urine collection container can lead to a false positive
blood result on the chemical reagent strip. Bleach can trigger the color change
normally associated with the presence of red blood cells or hemoglobin. It is
important to follow up with a microscopic analysis for confirmation of a true positive
result.
Question Difficulty: Level 7
A test for microalbuminuria is used most often to monitor patients with which of the
following diseases or conditions?
Diabetes
Bacterial infection
Multiple myeloma
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Tests for microalbuminuria are used most often to monitor patients with type 1 or type
2 diabetes.
Cells seen in the microscopic examination of the urine of a 30 year old female which
are smaller than RBCs but much the same shape are probably:
yeast cells
small lymphocytes
squamous epithelial cells
trichomonas vaginalis
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Yeast cells can appear very similar to red blood cells in the urine at times. Yeast cells
are a bit smaller than red blood cells and can have a "budding" appearance. This
morphology can aid in the differentiation between red cells and yeast.
Cholesterol crystals
Uric acid crystals
Amorphous urate crystals
Cystine crystals
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Cystine crystals are characteristically seen as colorless hexagonal plates in acid urine.
They may be confused with hexagonal uric acid crystals. They can be differentiated
from uric acid by their solubility in dilute hydrochloric acid versus crystalline uric
acid, which is not soluble in dilute hydrochloric acid. The cyanide-nitroprusside test
can be used to confirm the presence of cystine in urine. Cystine crystals are not
present in normal urine.
All the following statements about the urine specimen are true EXCEPT:
The urine specimen should be tested within two hours of collection if the
specimen is stored at room temperature.
The urine specimen should be at room temperature prior to testing.
The urine specimen should always be centrifuged before performing the
chemical reagent strip testing.
The urine specimen must be well-mixed before performing the chemical reagent
strip testing.
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The intended answer is, "The urine specimen should always be centrifuged before
performing the dipstick testing." This is not the case. Urine specimens are not to be
centrifuged prior to chemical reagent strip analysis.
Triple phosphate
Ammonium biurate
Calcium oxalate
Leucine
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Crystalline uric acid and calcium oxalates can be found in normal acid urine.
Ammonium biurate and triple phosphate can be found in normal alkaline urine.
Leucine crystals are abnormal, and can be found in maple syrup urine disease.
The image is a stained smear of cerebrospinal fluid. What types of cells are present in
this field?
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These are both normal cells that are not malignant. Macrophages can appear in the
CSF as well as arachnoid mesothelial cells. There are no neutrophils present in this
image.
Bacterial contamination
Urine that has remained at room temperature for an extended period of time
Alkaline medication
Ascorbic acid
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Bacterial contamination, urine that has remained at room temperature for an extended
period of time, and the presence of alkaline medication are all factors which can cause
false positives on the protein pad of a urine reagent strip.
Phase contrast
Darkfield microscopy
Brightfield microscopy
Polarized light
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A polarizing microscope with a first order red compensator is used to identify crystals
in synovial fluid: Needle shaped monosodium urate crystals are strongly negatively
birefringent, while rhomboid calcium pyrophosphate crystals are weakly positively
birefringent.
Amyloidosis
Nephrotic syndrome
Multiple myeloma
Macroglobulinemia
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Bence-Jones proteins are monoclonal light chains excreted in the urine, seen with
multiple myeloma, and other neoplasms of lymphoid cells. Small amounts can be
missed by urine dipsticks, which are more sensitive to albumin than globulins. They
are best detected by urine protein electrophoresis, immunoelectrophoresis, or
immunofixation. Large amounts cause renal tubular damage, resulting in myeloma
kidney. Bence-Jones proteins have unusual heat solubility properties, in that they
precipitate between 50- 60 degrees C and redisolve at 90 -100 degrees C. Historical
detection methods often utilized this unusual property.
Which of the following statements are TRUE for specific gravity measured by the
urine chemical reagent strip method? (Select ALL that apply)
The urine chemical reagent strip method is based on the relationship between
ionic concentration and specific gravity.
The urine chemical reagent strip method is based on the fact that light is
refracted in proportion to the amount of total solids dissolved in the urine.
The urine chemical reagent strip method is not affected by the presence of x-ray
contrast media in the urine specimen.
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There are two correct statements listed in the answer choices which accurately
describe specific gravity measurements by the reagent strip method. These statements
are:
The urine chemical reagent strip method is based on the relationship between ionic
concentration and specific gravity.
AND
The urine chemical reagent strip method is not affected by the presence of x-ray
contrast media in the urine specimen.
Which of the following tests included on a urine reagent strip would never be reported
out as negative?
Urobilinogen
Bilirubin
Leukocyte esterase
Blood
Nitrite
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True or false? The element that is indicated by the arrow is a red blood cell cast.
True
False
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This is a red blood cell cast. Red blood cells (RBCs) can be identified within the
matrix of the cast. The identification of the cast as a red blood cell cast is supported
by the appearance of RBCs outside of the cast.
The presence of only slightly visible hemolysis will significantly increase the serum
level of which of the following electrolytes?
Sodium
Potassium
Chloride
Bicarbonate
CO2
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Potassium, since it is a major intracellular cation, is spilled into the blood when red
blood cells are ruptured and broken, such as during hemolysis.
Sperm count
Morphology examination
Viscosity assessment
Glucose concentration
Motility
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What term is used to describe the color in these tubes of cerebrospinal fluid (CSF)?
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The term that is used to describe the color in these tubes of CSF is "xanthochromia."
Xanthochromia is an abnormal color, usually yellow, orange, or pink, in the
supernatant of the CSF sample. It may indicate that a subarachnoid hemorrhage
(SAH) has occurred.
Jaundice and icterus both describe a yellowing of the skin, mucous membranes, and
eyes. Blood plasma/serum that is deep yellow is also described as icteric.
Phase contrast
Darkfield microscopy
Brightfield microscopy
Polarized light
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A polarizing microscope with a first order red compensator is used to identify crystals
in synovial fluid: Needle shaped monosodium urate crystals are strongly negatively
birefringent, while rhomboid calcium pyrophosphate crystals are weakly positively
birefringent.
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Since the recommended time limit between collection and analysis is one hour, at
three hours, the technologist should report the specimen as compromised on the final
report.
What is the lower reference limit for semen volume according to the World Health
Organization, WHO Laboratory Manual for the Examination and Processing of
Human Semen, 5th edition?
0.5 mL
1.5 mL
3.5 mL
4.5 mL
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A plasma cortisol of 22 g/dL at 8:30 a.m. and 10 g/dL at 4:00 p.m. would be:
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The amount of cortisol present in the blood undergoes diurnal variation, with the
highest levels present in the early morning, and the lowest levels present around
midnight, or 35 hours after the onset of sleep. Addison's disease will show lower
levels of cortisol and other steroid hormones without fluctuation in the day/night.
Cushing's syndrome is associated with high levels of cortisol throughout the day and
night. Pheochromocytoma is associated with an increased release of epinephrine and
norepinephrine.
What is generally accepted as the lower threshold value for semen pH from fertile
males?
5.5
6.5
7.2
8.0
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The lower threshold value of semen pH from fertile males is generally accepted to be
7.2.
All of the statements below about synovial fluid are true EXCEPT:
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Synovial fluid is actually quite viscous and does not have the same consistency as
plasma. The reason for its thicker consistency is to provide cushion and reduce
friction between the articular cartilage of synovial joints during movement.
1:2
1:20
1:200
1:400
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Prostate
Pituitary gland
Seminal vesicles
Bulbourethral gland
Urethra
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The structures involved in the production of semen include the prostate, the seminal
vesicles, and the bulbourethral gland, along with the testes and epididymis.
Semen is produced as a combination of secretions from the different regions of the
male reproductive tract. Each fraction differs in chemical composition and function.
Spermatozoa are produced in the testes. They mature in the epididymis. The
testes also produce testosterone and inhibin.
Fluid from the seminal vesicles accounts for approximately 70% of semen
volume. The seminal vesicles are the source of fructose in semen. Fructose is
used by the spermatozoa as an energy source.
The prostate gland supplies about 20% of the volume of semen. Its fluids
include acid phosphatase and proteolytic enzymes that lead to coagulation and
subsequent liquefaction of semen. The prostate also contains most of the IgA
found in semen.
The bulbourethral gland produces mucoproteins that make up about 5% of the
volume of semen.
The pituitary gland is not directly involved in the production of semen; instead
hormones are released which stimulate the production of sperm. The urethra is not
involved in the production of semen.
Question Difficulty: Level 7
What is the lower reference limit for progressive motility of spermatozoa when
performing a semen analysis?
12%
22%
32%
40%
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The lower reference limit for progressive motility is 32%. The lower reference limit
for total motility (progressive + non-progressive) is 40 %.
A CSF glucose value is 62 mg/dL. What would you estimate the serum glucose?
65 mg/dL
93 mg/dL
120 mg/dL
180 mg/dL
41 mg/dL
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CSF glucose is roughly estimated to be about 2/3 of the serum glucose level in mg/dL.
Question Difficulty: Level 6
3.80
67.5
13.5
300
540
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*Note in this question both sides were counted without separate values given for the
average to be obtained, therefore substitute the total cell count in the numerator with
the total squares counted in the denominator
30 minutes
2 hours
4 hours
8 hours
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According to the 5th edition of the WHO Laboratory Manual for the Examination and
Processing of Human Semen, the analysis should begin at 30 minutes (if liquifaction
is complete), but no longer than one hour after ejaculation. Occasionally a specimen
does not liquify. If this occurs, mechanical mixing or enzyme treatment may be
necessary in order for the sperm count, motility analysis and other microscopic
aspects of semen analysis to be performed.
Match the following terms with the statement that best describes each:
Your answers are on the left. The correct answers are on the right and highlighted.
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Normal ratio of serum to CSF Protein is about 200 to 1, or less than 1%. Elevation of
CSF total protein is a nonspecific finding, but usually indicates some type of disease
involving the brain or meninges.
For BEST results, a semen sample should remain at which of the following
temperatures following collection?
4 C
Room temperature
37 C
56 C
-40 C
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True
False
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This statement is false. According to WHO 5th edition, the lower reference limit for
sperm concentration is 15 x 106 spermatozoa per ml.
On a Cytospin preparation from a pleural fluid, 50% of the cells have the following
characteristics:
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Mesothelial cells have a few key morphological features that aid in identification;
notablly the "fried egg" appearance.
Charcot-Leyden crystals in stool may be associated with an immune response and are
thought to be the breakdown products of:
neutrophils
eosinophils
monocytes
lymphocytes
basophils
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======
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True
False
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A 62-year-old man drank an unknown liquid in a suicide attempt, and his urine
contained crystals similar in shape to those shown in this image. This man MOST
likely ingested:
Methanol
Isopropanol
Ethylene glycol
Ethanol
Rubbing alcohol
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The crystals that are seen in the urine are monohydrate calcium oxalate crystals.
Oxalic acid, a byproduct of ethylene glycol metabolism, combines with calcium in the
body to form monohydrate calcium oxalate crystals, as shown in the accompanying
image.
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True Statements:
Urine should be well mixed prior to dipping the reagent strip. Prolonged immersion
may wash out test reagents.
False Statements:
Urine should be centrifuged prior to dipping the reagent strip. When visually reading
the reagent strip, all results can be read immediately after dipping the strip in the urine
specimen.
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If you notice a potentially hazardous situation in the laboratory that is out of your
control to fix, notify your supervisor or laboratory administration. It is important to
have the situation addressed and corrected if necessary. Warning other employees to
be careful may prevent injuries temporarily, but will not fix the hazardous situation.
If administration does not respond to your concerns, you may need to contact the
laboratory's accrediting agency, but this should not be your first step in addressing the
potentially hazardous situation.
Which of the following statements are true for the reagent strip procedure? (Choose
ALL correct answers)
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Prolonged immersion of the strip in the specimen may wash out reagents and affect
test results. Some tests on the reagent strip require specified reading times. If you are
visually reading the results, it is important to adhere to the timing recommended by
the reagent strip manufacturer.
The edge of the strip should be run along the lip of the specimen container or touched
to absorbent toweling, but should not be blotted with a paper towel.
Expiration dates indicate the ensured reactivity of the reagent strips. Even though
controls may be accurate, it does not guarantee that patient results will be accurate if
the reagents are beyond the assigned expiration date.
Budding yeast
Cylindroids
Trichomonas
Spermatozoa
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