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EXPERIMENT 1

stratified squamous epithelial cells

albumen: acts as adhesive

fixative: absolute alcohol

stain: methylene blue

dehydrate: acetone-alcphol

clearing: xylol

mount: balsam

tissues were taken from the cheek beause it is easy to obtain

Methylene blue- negatively charged molecules, including DNA and RNA

EXPERIMENT 2
if the hematocrit is increased, the angle of the spreader decreased.

If the hematocrit is decreased, the angle of the spreader increased.

chemical components of wright stain

oxidized methylene blue and azure- stains nucleus


eosin y - orange to pink color of cells cytopasm

romanowsky stain are already prepared in methyl alcohol, combinging fization


and staining at the same time

erythrocytes- dark pink

eosinophils- red granules

neutrophils- violet or pink

lymphocytes- blue cytoplasm

monocyte- grey to blue


basophil- blue

platelets- violet

buer water: itensify staining

EXPERIMENT 3
Fixative: Farmers fluid

HCL: dissolve pectic substance

stain: acetocarmine

destain: glacial acetic acid

mount :parafin

distal root tips os used because it is actively dividing

young root tip are square

mature roots are rectangular

EXPERIMENT 4
stain: eosin y

dehydrate- 50,70,80,90 alcohol

clearing: xylol

moount: balsam

naoh and kaoh to dissolve tissues

Experiment 5
stain: silver nitrate

sodium thiosulfate: remove excess agno

dehydrate: absolute alcohol

clear: carbon tetrachloride

mount :balsam

carborundum hone should be wet to prevent friction

partially closed diaphragm because opening it fully will be too bright and the
structures cant be seen

CHEEK CELLS BLOOD ALIUM CEPA PLACOID BONE

Fixation Absolute alcohol Farmers Fluid

Dehydration Acetone-Alcohol Absolute Alcohol

Clearing Xylol Xylol CCl4

Staining Metyhlene Blue Wrights Stain Acetocarmine Eosin Y Silver Nitrate

Mounting Balsam Balsam Paraffin Balsam Balsam