Histotechniques

Study online at quizlet.com/_1gfc6r

1. Two methods of preparing Cold knife & Cryostat 24. Zenker's Fluid components Glacial acetic acid
Frozen Section procedure Mercury chloride
2. Cold knife temp -40 to -60 deg C 25. Glacial acetic acid pH 4.6 or less
3. Cryostat opt working temp -18 to -20 deg C 26. Glacial acetic acid preserves Nucleoproteins / nuclear
structures
4. Cryostat procedure microtome Rotary microtome
27. Glacial acetic acid destroys Mitochondria and golgi
5. Water Mounting media for Cryostat
bodies
sections
28. Newcomer's fluid Both nuclear and
6. 20-30% bovine albumin Mounting media for Cryostat
histochemical fixative
sections
29. Best general tissue fixative 10% BNF
7. Von apathy's gum syrup Mounting media for Cryostat
sections 30. 10% Formaldehyde artefacts Paraformaldehyde
Acid formaldehyde
8. O.C.T. Best Mounting media for
hematin
Cryostat sections
31. Paraformaldehyde White crystalline ppts
9. Liquid Nitrogen Most Rapid Freezing agent
32. Parafomaldehyde removal Filtration or
10. Commonly used methods of Liquid nitrogen
10% methanol
freezing Isopentane cooled by liquid
nitrogen 33. Acid formaldehyde hematin Brown/black granules
CO2 gas
34. Acid formaldehyde hematin Kardasewitsch method
Aerosol spray
removal Lillie's method
11. Staining methods for frozen H&E (Progressive) Saturated alcoholic picric
section Thionine acid
Polychrome methylene blue Alcoholic KOH
Alcoholic pinacyanol method
35. 10% Formol Saline CNS tissues
12. Kind of H&E staining for frozen Progressive staining
36. Glutaraldehyde ELECTRON
section
MICROSCOPY
13. Tissue processing Fixation Enzyme histochemistry
F DC IETS SML Dehydration
37. Formol-Calcium Lipids
Clearing
Impregnation 38. Karnovsky's paraformaldehyde- Electron cytochemistry
Embedding glutaraldehyde soln
Trimming 39. Acrolein Electron cytochemistry
Sectioning
Staining 40. Most common metallic fixative Mercuric chloride
Mounting 41. Heidenhain's SuSa Tumor biopsies of the
Labeling skin
14. First and most critical step in Fixation 42. B-5 Fixative Bone Marrow biopsies
histotechnology
43. Chromate artifacts Fine yellow brown
15. Primary aim of Fixation Preserve morphology and deposits
chemical integrity
44. Chromate artifact removal Acid Alcohol
16. Secondary aim of fixation To harden tissue and protect
45. Orth's fluid Ricketssia and other
from trauma
bacteria
17. Correct fixative to tissue ratio 20:1
46. Orth's fluid Early degenerative
18. Usual fixation time 24 hours process
19. Surgical specimen fixation Room temp 47. Lead fixatives Acid
temperature mucopolysaccharides
20. E/M and histo hem fixation 0 to 4 deg C (Wharton's jelly)
temp 48. Bouin's solution EMBRYO
21. Fixation pH 6 to 8 Pituitary biopsy

22. Glacial Acetic Acid effect Causes tissue to SWELL 49. Brasil's Alcoholic Picroformol Excellent for GLYCOGEN

23. Mercury chloride effect Causes tissue to SHRINK 50. Glacial Acetic Acid solidifies 17 deg C
51. Methanol Blood smears 76. Von Ebner's fluid Hydrochloric acid decalcifying
Bone marrow smears agent
52. Ethanol Cytological smears 77. Dehydration aim Remove fixative and water from
(Pap's) tissue
53. Ethanol causes Polarization of glycogen 78. Most common Alcohol
dehydrating agent
54. Most rapid fixative Carnoy's fluid
79. Grades of alcohol are Ascending grades of Alcohol
55. Carnoy's fluid uses Urgent biopsies
used in Dehydration
(Lymph node and glycogen)
Rabies Dx 80. Ethanol Routine dehydration of tissues
Chromosome fixation
81. Best dehydrating agent Ethanol
56. Alcoholic formalin Gendre's fixative
82. Methanol Blood and tissue films
57. Alcoholic formalin preseves Sputum
83. Butyl alcohol Plant and animal microtechniques
58. Newcomer's fluid preserves Nuclear proteins
84. Acetone Fixative
Mucopolysaccharides
Dehydrating agent
59. Osmium Tetroxide fixatives Flemming's
85. Both dehydrating and Dioxane
60. Flemming's solution Nuclear structures clearing agents THF (tetrahydrofuran)
61. Flemming's solution without Cytoplasmic structures 86. Clearing Dealcoholization
acetic acid
87. Clearing agent miscible Miscible with dehydrating fluid
62. Trichloroacetic acid Fixative and impregnating medium
Decalcifying agent
88. Xylene Most common clearing agent
63. Acetone optimum Ice cold Xylol
temperature -5 to 4 deg C
89. Xylene Most rapid clearing agent
64. Acetone uses Water diffusible enzymes Xylol
(phosphate & lipase)
90. Chloroform Clearing tough specimens
Rabies Dx
91. Aniline oil Clearing very delicate tissue spx,
65. Heat Fixation Bacterial smears
embryo, insects
66. Microwave fixation optimum 45 to 55 deg C
92. Paraffin Most common and BEST
temp
infiltrating medium
67. Rabies Dx fixatives Carnoy's fluid
93. Temperature of paraffin 55 to 60 deg C
Acetone
oven
68. Fixatives for Electron Glutaraldehyde
94. Paraffin oven maintained 2 to 5 deg C above MP of
microscopy Platinic chloride
paraffin
Platinic chloride-formalin
(Zamboni's) 95. Ester Wax Soluble in 95% ETOH
Gold chloride and other clearing agents
Osmium tetroxide Impregnation without clearing
10% BNF 96. Carbowax Most common water soluble wax
69. Decalcification fluid:tissue 20:1 97. Carbowax charac No dehydration and clearing
ratio needed
70. Decalcification opt temp RT (18-30 deg C) 98. Carbowax use Enzyme histochemistry
71. Decalcification ideal 24 to 48 hours 99. Bioloid Recommended embedding
duration medium for EYES
72. Most common decalcifying Nitric Acid 100. Celloidin Large hollow cavities
agent
101. Wet celloidin Bones
73. Most rapid decalcifying Phloroglucin-Nitric Acid Teeth
agent Large brain section
74. Best general decalcifying 5% Formic Acid Whole organs
agent 102. Dry celloidin Eyes
75. Small pieces of bones and Formic Acid 103. Gelatin impt when Dehydration is to be avoided
teeth decal agt HCl
104. Gelatin use Histochem
Enzyme studies
Frozen sections
Delicate specimens
105. Plastic / Resin Electron microscopy
106. Temperature of melted paraffin for Embedding 5 to 10 deg C above MP of paraffin
107. Routine Histology sectioninf 4-6 u
108. Frozen section thickness 10-15 u
109. Electron microscopy thickness 0.5 u
110. Most common microtome Rotary Microtome
111. Rocking microtome inventor & year Paldwell Trefall
1881
112. Rotary Microtome inventor & year Minot 1885-1886
113. Sliding Microtome inventor & year Adams
1789
114. Alzheimer's fixative Bodian's
Sevier Munger